Platform Dependent Verification: On Engineering Verification Tools for 21st Century

The paper overviews recent developments in platform-dependent explicit-state LTL model checking.Comment: In Proceedings PDMC 2011, arXiv:1111.006

Cystatin B Involvement in Synapse Physiology of Rodent Brains and Human Cerebral Organoids

Cystatin B (CSTB) is a ubiquitous protein belonging to a superfamily of protease inhibitors. CSTB may play a critical role in brain physiology because its mutations cause progressive myoclonic epilepsy-1A (EPM1A), the most common form of progressive myoclonic epilepsy. However, the molecular mechanisms underlying the role of CSTB in the central nervous system (CNS) are largely unknown. To investigate the possible involvement of CSTB in the synaptic plasticity, we analyzed its expression in synaptosomes as a model system in studying the physiology of the synaptic regions of the CNS. We found that CSTB is not only present in the synaptosomes isolated from rat and mouse brain cortex, but also secreted into the medium in a depolarization-controlled manner. In addition, using biorthogonal noncanonical amino acid tagging (BONCAT) procedure, we demonstrated, for the first time, that CSTB is locally synthesized in the synaptosomes. The synaptic localization of CSTB was confirmed in a human 3D model of cortical development, namely cerebral organoids. Altogether, these results suggest that CSTB may play a role in the brain plasticity and open a new perspective in studying the involvement of CSTB deregulation in neurodegenerative and neuropsychiatric diseases.Cystatin B (CSTB) is a ubiquitous protein belonging to a superfamily of protease inhibitors. CSTB may play a critical role in brain physiology because its mutations cause progressive myoclonic epilepsy-1A (EPM1A), the most common form of progressive myoclonic epilepsy. However, the molecular mechanisms underlying the role of CSTB in the central nervous system (CNS) are largely unknown. To investigate the possible involvement of CSTB in the synaptic plasticity, we analyzed its expression in synaptosomes as a model system in studying the physiology of the synaptic regions of the CNS. We found that CSTB is not only present in the synaptosomes isolated from rat and mouse brain cortex, but also secreted into the medium in a depolarization-controlled manner. In addition, using biorthogonal noncanonical amino acid tagging (BONCAT) procedure, we demonstrated, for the first time, that CSTB is locally synthesized in the synaptosomes. The synaptic localization of CSTB was confirmed in a human 3D model of cortical development, namely cerebral organoids. Altogether, these results suggest that CSTB may play a role in the brain plasticity and open a new perspective in studying the involvement of CSTB deregulation in neurodegenerative and neuropsychiatric diseases

Estudos sobre a nutrição mineral do arroz: VIII. marcha de absorção de macronutrientes na variedade IAC-25

Rice plants, IAC 25 variety, were grown in nutrient solution; samples were taken for analysis at different intervals, namely 21, 50, 79, 91 and 112 days after germination. Main conclusions were the following: (1) both dry matter production and accumulation of macronutrients as a function of time were described by typical sigmoide curves; (2) in the period between the second and third sampling the rate for both processes reached maximum values; (3) dilution effects were observed in the content of all the elements except Ca; (4) Ca also was the only macronutrien which does not seem to be translocated from leaves for grainfiling.Foram estudadas, em condições controladas, a acumulação de matéria seca e a acumulação de macronutrientes pela variedade de arroz IAC 25. Como regra verificou-se que a acumulação de matéria seca e de macronutrientes obedeceu a sigmoides típicas, ambos os processos ocorendo com maior velocidade no período aproximado de 5O-9O dias após a germinação

Hierarchical Adaptive State Space Caching based on Level Sampling

Abstract. In the past, several attempts have been made to deal with the state space explosion problem by equipping a depth-first search (DFS) algorithm with a state cache, or by avoiding collision detection, thereby keeping the state hash table at a fixed size. Most of these attempts are tailored specifically for DFS, and are often not guaranteed to terminate and/or to exhaustively visit all the states. In this paper, we propose a general framework of hierarchical caches which can also be used by breadth-first searches (BFS). Our method, based on an adequate sampling of BFS levels during the traversal, guarantees that the BFS terminates and traverses all transitions of the state space. We define several (static or adaptive) configurations of hierarchical caches and we study experimentally their effectiveness on benchmark examples of state spaces and on several communication protocols, using a generic implementation of the cache framework that we developed within the CADP toolbox.