3,025 research outputs found
Synthesis of CdTe quantum dot-conjugated CC49 and their application for in vitro imaging of gastric adenocarcinoma cells
The purpose of this experiment was to investigate the visible imaging of gastric adenocarcinoma cells in vitro by targeting tumor-associated glycoprotein 72 (TAG-72) with near-infrared quantum dots (QDs). QDs with an emission wavelength of about 550 to 780 nm were conjugated to CC49 monoclonal antibodies against TAG-72, resulting in a probe named as CC49-QDs. A gastric adenocarcinoma cell line (MGC80-3) expressing high levels of TAG-72 was cultured for fluorescence imaging, and a gastric epithelial cell line (GES-1) was used for the negative control group. Transmission electron microscopy indicated that the average diameter of CC49-QDs was 0.2 nm higher compared with that of the primary QDs. Also, fluorescence spectrum analysis indicated that the CC49-QDs did not have different optical properties compared to the primary QDs. Immunohistochemical examination and in vitro fluorescence imaging of the tumors showed that the CC49-QDs probe could bind TAG-72 expressed on MGC80-3 cells
Knowing What, How and Why: A Near Complete Solution for Aspect-based Sentiment Analysis
Target-based sentiment analysis or aspect-based sentiment analysis (ABSA)
refers to addressing various sentiment analysis tasks at a fine-grained level,
which includes but is not limited to aspect extraction, aspect sentiment
classification, and opinion extraction. There exist many solvers of the above
individual subtasks or a combination of two subtasks, and they can work
together to tell a complete story, i.e. the discussed aspect, the sentiment on
it, and the cause of the sentiment. However, no previous ABSA research tried to
provide a complete solution in one shot. In this paper, we introduce a new
subtask under ABSA, named aspect sentiment triplet extraction (ASTE).
Particularly, a solver of this task needs to extract triplets (What, How, Why)
from the inputs, which show WHAT the targeted aspects are, HOW their sentiment
polarities are and WHY they have such polarities (i.e. opinion reasons). For
instance, one triplet from "Waiters are very friendly and the pasta is simply
average" could be ('Waiters', positive, 'friendly'). We propose a two-stage
framework to address this task. The first stage predicts what, how and why in a
unified model, and then the second stage pairs up the predicted what (how) and
why from the first stage to output triplets. In the experiments, our framework
has set a benchmark performance in this novel triplet extraction task.
Meanwhile, it outperforms a few strong baselines adapted from state-of-the-art
related methods.Comment: This paper is accepted in AAAI 202
E17110 promotes reverse cholesterol transport with liver X receptor β agonist activity in vitro
AbstractLiver X receptor (LXR) plays an important role in reverse cholesterol transport (RCT), and activation of LXR could reduce atherosclerosis. In the present study we used a cell-based screening method to identify new potential LXRβ agonists. A novel benzofuran-2-carboxylate derivative was identified with LXRβ agonist activity: E17110 showed a significant activation effect on LXRβ with an EC50 value of 0.72μmol/L. E17110 also increased the expression of ATP-binding cassette transporter A1 (ABCA1) and G1 (ABCG1) in RAW264.7 macrophages. Moreover, E17110 significantly reduced cellular lipid accumulation and promoted cholesterol efflux in RAW264.7 macrophages. Interestingly, we found that the key amino acids in the LXRβ ligand-binding domain had distinct interactions with E17110 as compared to TO901317. These results suggest that E17110 was identified as a novel compound with LXRβ agonist activity in vitro via screening, and could be developed as a potential anti-atherosclerotic lead compound
Chinese herbal medicine shenqi detoxification granule inhibitsfibrosis in adenine induced chronic renal failure rats
Background: Progressive fibrosis accompanies all chronic renal disease, connective tissue growth factor (CTGF,) and platelet-derived growth factor-B, (PDGF-B,) play important roles in extra-cellular matrix abnormal accumulation, while endothelin -1 (ET-1) nitric oxide (NO,) are related to endothelial dysfunction, which mediates the progression of renal fibrosis. Shenqi Detoxification Granule (SDG), a traditional Chinese herbal formula, has been used for treatment of chronic renal failure in clinic for many years.Materials and Methods: In order to evaluate the efficacy, and explore the mechanism of SDG to inhibit the progression of renal fibrosis, study was carried out using the adenine-induced Wister rats as the CRF model, and losartan as postive control drug. Levels of serum creatinine [Scr], and blood urea nitrogen (BUN), albumin (ALB), 24hrs, urine protein (24hUP), triacylglycerol (TG), and cholesterol (CHO), together with ET-1, and NO were detected. Pathological changes of renal tissues were observed by HE, staining. In addition, CTGF and PDGF-B expression were analyzed by immuno-histo-chemistry.Results:The results indicated that SDG can effectively reduce Scr, BUN, 24hUP, TG, and CHO levels, increase ALB levels, inhibit renal tissue damage in CRF rats, and the mechanism maybe reduce PDGF-B, CTGF expression and ET-1/NO.Conclusion: Shenqi Detoxification Granule is a beneficial treatment for chronic renal failure.Key words: Chronic Renal Failure; fibrosis; Shenqi Detoxification Granule; CTGF; PDGF-B
Ecosystem-inspired model and artificial intelligence predicts pollutant consumption capacity by coagulation in drinking water treatment
Quantum delayed-choice experiment with a beam splitter in a quantum superposition
A quantum system can behave as a wave or as a particle, depending on the
experimental arrangement. When for example measuring a photon using a
Mach-Zehnder interferometer, the photon acts as a wave if the second
beam-splitter is inserted, but as a particle if this beam-splitter is omitted.
The decision of whether or not to insert this beam-splitter can be made after
the photon has entered the interferometer, as in Wheeler's famous
delayed-choice thought experiment. In recent quantum versions of this
experiment, this decision is controlled by a quantum ancilla, while the beam
splitter is itself still a classical object. Here we propose and realize a
variant of the quantum delayed-choice experiment. We configure a
superconducting quantum circuit as a Ramsey interferometer, where the element
that acts as the first beam-splitter can be put in a quantum superposition of
its active and inactive states, as verified by the negative values of its
Wigner function. We show that this enables the wave and particle aspects of the
system to be observed with a single setup, without involving an ancilla that is
not itself a part of the interferometer. We also study the transition of this
quantum beam-splitter from a quantum to a classical object due to decoherence,
as observed by monitoring the interferometer output.Comment: 9 pages, 7 figures, Accepted by Physical Review Letter
Active beam steering enabled by photonic crystal surface emitting laser
Emitting light towards on-demand directions is important for various
optoelectronic applications, such as optical communication, displaying, and
ranging. However, almost all existing directional emitters are assemblies of
passive optical antennae and external light sources, which are usually bulky,
fragile, and with unendurable loss of light power. Here we theoretically
propose and experimentally demonstrate a new conceptual design of directional
emitter, by using a single surface-emitting laser source itself to achieve
dynamically controlled beam steering. The laser is built on photonic crystals
that operates near the band edges in the continuum. By shrinking laser sizes
into tens-of-wavelength, the optical modes quantize in three-dimensional
momentum space, and each of them directionally radiates towards the far-field.
Further utilizing the luminescence spectrum shifting effect under current
injection, we consecutively select a sequence of modes into lasing action and
show the laser maintaining in single mode operation with linewidths at a
minimum of MHz and emitting power of ten milliwatts, and we
demonstrate fast beam steering across a range of in
a time scale of nanoseconds. Our work proposes a novel method for on-chip
active beam steering, which could pave the way for the development of
automotive, industrial, and robotic applications.Comment: 23 pages, 5 figure
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