Estado comparativo de las masas de Pinus uncinata Ram. potencialmente protectoras frente a aludes de una zona de Andorra y Cataluña

A comparative study was carried out among stands of Pinus uncinata Ram. located, respectively, in La Massana (Andorra) and in the Catalonian Pyrenees, and give these sites an important role against snow avalanches. To check if there exist some differential attributes between Andorran and Catalonian sites, a dendrometric database was generated from sampling (Andorra) and from the information provided by the Third Forest National Inventory (Catalonian Pyrenees). As for their tendency towards uneven-aged stand structure, both geographical regions are quite similar. In fact, stand regeneration is not associated to other stocking variables, and stand stocking is not closely linked to the altitudinal gradient (which little affects mortality). However, Catalonian stands have shown less stocking and, in general, they offer worse characteristics for their protective role against avalanches compared with the Andorran stands.Se aborda el estudio comparativo entre las masas de Pinus uncinata Ram., localizadas en la parroquia La Massana de Andorra y en el Pirineo catalán, desde la perspectiva de su potencial papel protector frente a aludes. La base de datos dasométricos se obtuvo, en el primer caso, de un inventario ad hoc, y en el segundo, de la información proporcionada por el Tercer Inventario Forestal Nacional. Los resultados sugieren que las masas de ambos entornos geográficos ofrecen aspectos comunes en cuanto a que muestran tendencia hacia la irregularidad, con una regeneración no ligada a las otras variables de masa y con una espesura poco condicionada por el gradiente altitudinal y que escasamente incide en la mortalidad. Las diferencias advertidas se centran en que en las masas de Cataluña se aprecia menores niveles de espesura, aunque aparecen estructuras capitalizadas en existencias volumétricas ausentes en Andorra, y que de modo general, ofrecen peores características en cuanto a su papel protector frente al desencadenamiento de avalanchas

Clinical significance of the detection of Candida albicans germ tube-specific antibodies in critically ill patients

AbstractThe present study, comprising a prospective multicentre study including 53 non-neutropenic patients from intensive care units (ICU) in six Spanish tertiary-care hospitals, was carried out to determine the clinical significance and influence on mortality of Candida albicans germ tube-specific antibodies (CAGTA). There were 22 patients (41.5%) for whom the CAGTA results were positive, although none of had a blood culture positive for Candida. The intra-ICU mortality rate was significantly lower (p = 0.004) in CAGTA-positive patients (61.2% vs. 22.7%). Multivariate analysis confirmed that a positive CAGTA result was the only protective factor to be independently associated with ICU mortality (β coefficient = −0.3856; 95% confidence interval = −0.648 to −0.123)

Elección de especies en las repoblaciones forestales. Contribuciones del profesor Ruiz de la Torre

Reforestation or afforestation is one of the most important management alternatives in ecological restoration. Selecting appropriate species is the main decision of a reforestation project, because the long-term implications of such decision in the management costs and value of the final community are significant. Both, factors and stages of selection process and theoretical concepts of forest restoration models are analyzed. In this paper, we review some of the contributions that Professor Ruiz de la Torre made to the knowing of silvical and ecological characteristics of the major tree species in Spain, and to the understanding of vegetation dynamics.La repoblación forestal es una de las alternativas técnicas más importantes en la restauración ecológica. La selección correcta de las especies es una de las decisiones más importantes del proyecto, por las implicaciones significativas que a largo plazo tiene en los costes de gestión y en el valor de la comunidad final. Se analizan los factores y las etapas del proceso de selección así como los conceptos teóricos que han servido de base a los modelos de restauración forestal. En este artículo presentamos algunas de las contribuciones que el Profesor Ruiz de la Torre ha realizado en relación al conocimiento de las características ecológicas y selvícolas de las principales especies arbóreas españolas, así como de los mecanismos de sucesión de la vegetación

Efecto del método de corte sobre el comportamiento a fatiga de un acero estructural de alta resistencia S690Q

Este artículo estudia el efecto de los diferentes métodos de corte térmico en la vida a fatiga de un acero estructural de alta resistencia S690Q. Se han seleccionado tres sistemas de corte térmico mecanizado ampliamente utilizados en la industria (oxicorte, corte por plasma y corte por láser) y dos detalles estructurales: bordes rectos cortados térmicamente y orificios obtenidos mediante corte térmico para su posterior empleo en uniones atornilladas. El programa experimental está constituido por 60 probetas, las cuales se han llevado a rotura por fatiga en una máquina de resonancia. El valor de la relación de tensiones (R) seleccionado es igual a 0.1. Por cada combinación de método de corte y detalle estructural se ha obtenido su correspondiente curva S-N y límite de fatiga. Se ha llevado a cabo un análisis de la rugosidad superficial y dureza de la Zona Afectada Térmicamente con objeto de determinar la influencia de los métodos de corte térmico en la vida en fatiga. Los resultados obtenidos se comparan con los valores de diseño propuestos por las normativas de diseño en fatiga, evaluando la posibilidad de extrapolar sus curvas S-N tanto a bordes rectos como agujeros cortados mediante cualquiera de los tres sistemas de corte térmico.This paper analyses the effect of different cutting methods on the fatigue life of high strength steel S690Q. The research covers three cutting methods (oxy-fuel, plasma and laser) and two specimen geometries: plain specimens with rectangular sections and cut edges, and specimens with machined edges and a cut hole in the middle section. All the specimens were conducted to failure by applying fatigue cycles, the stress ratio (R) being 0.1, and the corresponding SN curves were obtained for each combination of cutting method and specimen geometry. Measurements of roughness and hardness have been performed in order to explain the influence of the cutting method on the fatigue life of this particular steel. Fatigue results have been compared with the predictions provided by current fatigue standards, analysing the possibility of extrapolating their S-N curves, focused on oxy-fuel cuts, to plasma and laser cuts.HIPERCUT: High Performance Cut Edges in Structural Steel Plates for Demanding Aplication

Epidemiological cutoff values for fluconazole, itraconazole, posaconazole, and voriconazole for six Candida species as determined by the colorimetric Sensititre YeastOne method

In the absence of clinical breakpoints (CBP), epidemiological cutoff values (ECVs) are useful to separate wild-type (WT) isolates (without mechanisms of resistance) from non-WT isolates (those that can harbor some resistance mechanisms), which is the goal of susceptibility tests. Sensititre YeastOne (SYO) is a widely used method to determine susceptibility of Candida spp. to antifungal agents. The CLSI CBP have been established, but not for the SYO method. The ECVs for four azoles, obtained using MIC distributions determined by the SYO method, were calculated via five methods (three statistical methods and based on the MIC50 and modal MIC). Respectively, the median ECVs (in mg/liter) of the five methods for fluconazole, itraconazole, posaconazole, and voriconazole (in parentheses: the percentage of isolates inhibited by MICs equal to or less than the ECVs; the number of isolates tested) were as follows: 2 (94.4%; 944), 0.5 (96.7%; 942), 0.25 (97.6%; 673), and 0.06 (96.7%; 849) for Candida albicans; 4 (86.1%; 642), 0.5 (99.4%; 642), 0.12 (93.9%; 392), and 0.06 (86.9%; 559) for C. parapsilosis; 8 (94.9%; 175), 1 (93.7%; 175), 2 (93.6%; 125), and 0.25 (90.4%; 167) for C. tropicalis; 128 (98.6%; 212), 4 (95.8%; 212), 4 (96.0%; 173), and 2 (98.5; 205) for C. glabrata; 256 (100%; 53), 1 (98.1%; 53), 1 (100%; 33), and 1 (97.9%; 48) for C. krusei; 4 (89.2%; 93), 0.5 (100%; 93), 0.25 (100%; 33), and 0.06 (87.7%; 73) for C. orthopsilosis. All methods included =94% of isolates and yielded similar ECVs (within 1 dilution). These ECVs would be suitable for monitoring emergence of isolates with reduced susceptibility by using the SYO method

Early Stepdown From Echinocandin to Fluconazole Treatment in Candidemia: A Post Hoc Analysis of Three Cohort Studies

[Background] There are no clear criteria for antifungal de-escalation after initial empirical treatments. We hypothesized that early de-escalation (ED) (within 5 days) to fluconazole is safe in fluconazole-susceptible candidemia with controlled source of infection.[Methods] This is a multicenter post hoc study that included consecutive patients from 3 prospective candidemia cohorts (2007–2016). The impact of ED and factors associated with mortality were assessed.[Results] Of 1023 candidemia episodes, 235 met inclusion criteria. Of these, 54 (23%) were classified as the ED group and 181 (77%) were classified as the non-ED group. ED was more common in catheter-related candidemia (51.9% vs 31.5%; P = .006) and episodes caused by Candida parapsilosis, yet it was less frequent in patients in the intensive care unit (24.1% vs 39.2%; P = .043), infections caused by Nakaseomyces glabrata (0% vs 9.9%; P = .016), and candidemia from an unknown source (24.1% vs 47%; P = .003). In the ED and non-ED groups, 30-day mortality was 11.1% and 29.8% (P = .006), respectively. Chronic obstructive pulmonary disease (odds ratio [OR], 3.97; 95% confidence interval [CI], 1.48–10.61), Pitt score > 2 (OR, 4.39; 95% CI, 1.94–9.20), unknown source of candidemia (OR, 2.59; 95% CI, 1.14–5.86), candidemia caused by Candida albicans (OR, 3.92; 95% CI, 1.48–10.61), and prior surgery (OR, 0.29; 95% CI, 0.08–0.97) were independent predictors of mortality. Similar results were found when a propensity score for receiving ED was incorporated into the model. ED had no significant impact on mortality (OR, 0.50; 95% CI, 0.16–1.53).[Conclusions] Early de-escalation is a safe strategy in patients with candidemia caused by fluconazole-susceptible strains with controlled source of bloodstream infection and hemodynamic stability. These results are important to apply antifungal stewardship strategies.This research forms part of an activity that has received funding from EIT Health. EIT Health is supported by the European Institute of Innovation and Technology (EIT), a body of the European Union that receives support from the European Union´s Horizon 2020 Research and Innovation Program. This study has been cofunded by the European Regional Development Fund. E. M.-G. (PI18/01061), P. P.-A. (“Rio Hortega” contract CM18/00132), M. F.-R. (“Miguel Servet” contract CP18/00073), and C. G.-V. (FIS PI18/01061) have received research grants from the Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III.Peer reviewe

Clinical factors associated with a Candida albicans Germ Tube Antibody positive test in Intensive Care Unit patients

Background: Poor outcomes of invasive candidiasis (IC) are associated with the difficulty in establishing the microbiological diagnosis at an early stage. New scores and laboratory tests have been developed in order to make an early therapeutic intervention in an attempt to reduce the high mortality associated with invasive fungal infections. Candida albicans IFA IgG has been recently commercialized for germ tube antibody detection (CAGTA). This test provides a rapid and simple diagnosis of IC (84.4% sensitivity and 94.7% specificity). The aim of this study is to identify the patients who could be benefited by the use of CAGTA test in critical care setting. Methods: A prospective, cohort, observational multicentre study was carried out in six medical/surgical Intensive care units (ICU) of tertiary-care Spanish hospitals. Candida albicans Germ Tube Antibody test was performed twice a week if predetermined risk factors were present, and serologically demonstrated candidiasis was considered if the testing serum dilution was >= 1: 160 in at least one sample and no other microbiological evidence of invasive candidiasis was found. Results: Fifty-three critically ill non-neutropenic patients (37.7% post surgery) were included. Twenty-two patients (41.5%) had CAGTA-positive results, none of them with positive blood culture for Candida. Neither corrected colonization index nor antifungal treatment had influence on CAGTA results. This finding could corroborate that the CAGTA may be an important biomarker to distinguish between colonization and infection in these patients. The presence of acute renal failure at the beginning of the study was more frequent in CAGTA-negative patients. Previous surgery was statistically more frequent in CAGTA-positive patients. Conclusions: This study identified previous surgery as the principal clinical factor associated with CAGTA-positive results and emphasises the utility of this promising technique, which was not influenced by high Candida colonization or antifungal treatment. Our results suggest that detection of CAGTA may be important for the diagnosis of invasive candidiasis in surgical patients admitted in ICU.This study has been supported by a Pfizer research gran

Multilocus microsatellite analysis of European and African Candida glabrata isolates

This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.This research was supported in part by the EU Mare Nostrum (EUMN-III Call) program of the European Union, grant agreement number 2011-4050/001-EMA2. Dr Sanae Rharmitt was the recipient of a scholarship (10 months) signed within the EUMN program for PhD students (F.S. 1.04.11.01 UORI) under the supervision of Prof Orazio Romeo.info:eu-repo/semantics/publishedVersio