Diet in neurogenic bowel management: a viewpoint on spinal cord injury

The aim of this review is to offer dietary advice for individuals with spinal cord injury (SCI) and neurogenic bowel dysfunction. With this in mind, we consider health conditions that are dependent on the level of lesion including skeletal muscle atrophy, autonomic dysreflexia and neurogenic bladder. In addition, SCI is often associated with a sedentary lifestyle, which increases risk for osteoporosis and diseases associated with chronic low-grade inflammation, including cardiovascular and chronic kidney diseases. The Mediterranean diet, along with exercise and dietary supplements, has been suggested as an anti-inflammatory intervention in individuals with SCI. However, individuals with chronic SCI have a daily intake of whole fruit, vegetables and whole grains lower than the recommended dietary allowance for the general population. Some studies have reported an increase in neurogenic bowel dysfunction symptoms after high fiber intake; therefore, this finding could explain the low consumption of plant foods. Low consumption of fibre induces dysbiosis, which is associated with both endotoxemia and inflammation. Dysbiosis can be reduced by exercise and diet in individuals with SCI. Therefore, to summarize our viewpoint, we developed a Mediterranean diet-based diet and exercise pyramid to integrate nutritional recommendations and exercise guidelines. Nutritional guidelines come from previously suggested recommendations for military veterans with disabilities and individuals with SCI, chronic kidney diseases, chronic pain and irritable bowel syndrome. We also considered the recent exercise guidelines and position stands for adults with SCI to improve muscle strength, flexibility and cardiorespiratory fitness and to obtain cardiometabolic benefits. Finally, dietary advice for Paralympic athletes is suggested

Immuno-Assessment of «Pseudomonas syringae» Lipodepsipeptides (Syringomycins and Syringopeptins)

Following a previous work on the immunological detection of syringopeptins (SPs), polyclonal antibodies with a high specificity for syringomycins (SRs) were raised in rabbits and purified. Assayed in a competitive ELISA, the most common forms of SR, i.e. SR-E and SR-G, were recognised with a detection limit of 0.1 mg per well, whereas other structurally related bacterial lipodepsipeptides (LDP), such as SPs, pseudomycins (PSs) and syringotoxins (STs) were not recognised. The immuno-assay (competitive ELISA) method developed in this work is about 100 times more sensitive than the current chromatographic (HPLC) method and requires no previous extraction of the toxin. The production of LDP in culture by strains of three pathovars of Pseudomonas syringae (pv. aptata, pv. lachrymans and pv. syringae) was found to range from 0.026 to 0.055 mg ml-1 for SRs and from 0.02 to 0.06 mg ml-1 for SPs. Both the concentration of LDP in aqueous extracts from zucchini cotyledons infected by P. syringae pv. lachrymans and the severity of symptoms were shown to increase progressively after infection. The immunologically estimated concentration of SRs in the infected cotyledons averaged 0.22 mg g-1 f wt after 12 hours, and 0.39 mg g-1 after 4 days. The corresponding values for SPs were 0.11 and 0.37 mg g-1. In a recovery experiment, solutions of pure toxins (0.22 mg SR-E and 0.14 mg SP25A g-1 f wt) were injected in healthy cotyledons. After 2 days, overestimation due to toxin complexing in planta was of 10% for (SR-E) and 40% for (SP25A). Applying these percentages to the values estimated for infected cotyledons, the net concentrations were as follows: 12 h after inoculation: 0.20 (SRs) and 0.07 (SPs) mg g-1 f wt; four days after inoculation: 0.35 (SRs) and 0.22 (SPs) mg g-1 f wt. The values obtained with aqueous extracts from infected plants are relatively high if compared to the figures of the in vitro experiments. It is assumed that the high reactivity of ELISA to the immune-LDP-related compounds present in the water extracts from infected plants is due to the presence of high molecular weight LDP complexes having a cross-reactivity with antibodies substantially higher than that of free toxins

Protection against Pseudomonas aeruginosa lung infection in mice by recombinant OprF-pulsed dendritic cell immunization

BACKGROUND: The Pseudomonas aeruginosa major constitutive outer membrane porin protein F (OprF) has been shown to be a protective antigen and was previously used to activate an immunological response in a mouse model of lung pneumonia. The purpose of our study was to demonstrate the ability of mouse dendritic cells pulsed with purified or recombinant OprF to protect mice against P. aeruginosa infection and inflammation.Both native (n-OprF), isolated and purified from PAO1 bacterial strain, and recombinant (histidin-conjugated) OprF (His-OprF), obtained by cloning of the oprF gene into the pET28a expression vector, were used to stimulate dendritic cells in vitro before adoptive transfer into prospective recipient mice with P. aeruginosa pulmonary infection. RESULTS: Similar to n-OprF, His-OprF activated dendritic cells in vitro, inducing the costimulatory molecule expression as well as cytokine production. Upon adoptive transfer in vivo, porin-pulsed dendritic cells (DCs) induced Th1-mediated resistance to infection and associated inflammatory pathology caused by either the PAO1 strain or a clinically-isolated mucoid strain. CONCLUSIONS: This study highlights the pivotal contribution of DCs to vaccine-induced protection against P. aeruginosa infection and associated inflammation

Risk factors for endocrine complications in transfusion-dependent thalassemia patients on chelation therapy with deferasirox: a risk assessment study from a multicentre nation-wide cohort

Transfusion-dependent patients typically develop iron-induced cardiomyopathy, liver disease, and endocrine complications. We aimed to estimate the incidence of endocrine disorders in transfusion-dependent thalassemia (TDT) patients during long-term iron-chelation therapy with deferasirox (DFX).We developed a multicentre follow-up study of 426 TDT patients treated with once-daily DFX for a median duration of 8 years, up to 18.5 years. At baseline, 118, 121, and 187 patients had 0, 1, or ≥2 endocrine diseases respectively. 104 additional endocrine diseases were developed during the follow-up. The overall risk of developing a new endocrine complication within 5 years was 9.7% (95%CI=6.3-13.1). Multiple Cox regression analysis identified 3 key predictors: age showed a positive log-linear effect (adjusted HR for 50% increase=1.2, 95%CI=1.1-1.3, P=0.005), the serum concentration of thyrotropin (TSH) showed a positive linear effect (adjusted HR for 1 mIU/L increase=1.3, 95%CI=1.1-1.4, P

Richieste info e statistiche di accesso al sito Web dell’Osservatorio Vesuviano (INGV) in relazione alla sequenza sismica dell’Abruzzo - aprile 2009

Immediatamente dopo l’evento di M 5.8 verificatosi in Abruzzo in prossimità dell’Aquila il 6 aprile 2009 alle ore 03:33 (ora locale) il sito web dell’Osservatorio Vesuviano (INGV) ha registrato un improvviso aumento degli accessi che, con diverse modulazioni, è perdurato per tutto il mese di aprile. Contemporaneamente numerose richieste di informazioni sono pervenute all’indirizzo di posta [email protected]. L’Osservatorio Vesuviano non ha prodotto sul proprio sito documenti specifici sull’evento maggiore né sulla sequenza dell’Aquilano, rimandando con un link alla pagina del portale nazionale. Dall’analisi delle statistiche degli accessi e dalle mail ricevute emerge che l’incremento degli accessi è stato determinato dalla consultazione dei “Segnali Sismici in Tempo Reale” in linea sul sito dal 2000. Dalla lettura delle mail si rileva che l’applicazione riscuote grande consenso presso gli utenti ed è recepita come un segnale di affidabilità e trasparenza dell’operato dell’intero Istituto.Istituto Nazionale di Geofisica e Vulcanologia, sezione di Napoli Osservatorio VesuvianoPublished1.1. TTC - Monitoraggio sismico del territorio nazionale5.10. TTC - Sistema webope

muography with nuclear emulsions stromboli and other projects

The muon radiography is a novel imaging technique to probe the volcanoes interior, using the capability of high energy cosmic ray muons to penetrate large thicknesses of rock. In this way it is possible to derive a 2D density map along the muon trajectory of volcanic edifices and deduce information on the variations in the rock density distribution, like those expected from dense lava conduits, or low density magma supply paths. This method is applicable also to study geological objects as glaciers, faults, oil underground reservoirs, engineering constructions, where a density contrast is present. Nuclear emulsions are well suited to be employed in this context for their excellent angular resolution; they are compact and robust detectors, able to work in harsh environments without need of power supply. On the other side, a long exposure time is required for a reasonable detector surface (~10 m 2 ) in order to collect a sufficient statistics of muons, and a quasi-real time analysis of the emulsion data is rather difficult due to the scanning time needed by the optical microscopes. Such drawback is on the way to be overcome thanks to a recent R&D program on ultra-fast scanning systems. Muon radiography technique, even if limited to the summit part of the volcano edifice, represents an important tool of investigation, at higher spatial resolution, complementary to the conventional geophysics techniques. The first successful result in this field was obtained by a Japanese group that observed in 2007 the conduit structure of Mt. Asama. Since 2010, other interesting volcanoes have been probed with the same method: Stromboli in 2011, Mt. Teide in 2012 and La Palma in 2014. Here we discuss the muon imaging technique reporting the nuclear emulsion detector design exposed at Stromboli and results of the data analysis

Richieste info e statistiche di accesso al sito Web dell’Osservatorio Vesuviano (INGV) in relazione alla sequenza sismica dell’Abruzzo - aprile 2009

Immediatamente dopo l’evento di M 5.8 verificatosi in Abruzzo in prossimità dell’Aquila il 6 aprile 2009 alle ore 03:33 (ora locale) il sito web dell’Osservatorio Vesuviano (INGV) ha registrato un improvviso aumento degli accessi che, con diverse modulazioni, è perdurato per tutto il mese di aprile. Contemporaneamente numerose richieste di informazioni sono pervenute all’indirizzo di posta [email protected]. L’Osservatorio Vesuviano non ha prodotto sul proprio sito documenti specifici sull’evento maggiore né sulla sequenza dell’Aquilano, rimandando con un link alla pagina del portale nazionale. Dall’analisi delle statistiche degli accessi e dalle mail ricevute emerge che l’incremento degli accessi è stato determinato dalla consultazione dei “Segnali Sismici in Tempo Reale” in linea sul sito dal 2000. Dalla lettura delle mail si rileva che l’applicazione riscuote grande consenso presso gli utenti ed è recepita come un segnale di affidabilità e trasparenza dell’operato dell’intero Istituto