29 research outputs found
Respuesta ecofisiológica y capacidad de fotoaclimatación de las praderas de Caulerpa prolifera (Forsskal) J.V. Lamouroux y Cymodocea nodosa (Ucria) Ascherson en el Mar Menor (Murcia, España)
Tras el ensanchamiento en 1972 del principal canal de comunicación con el Mediterráneo, la macroalga Caulerpa prolifera colonizó el Mar Menor. Durante más de tres décadas, su expansión gradual ha reducido las praderas de Cymodocea nodosa, existentes en la laguna, a manchas dispersas en zonas arenosas someras. El objetivo principal es describir la respuesta ecofisiológica y la capacidad de aclimatación de ambas especies con el fin de determinar la influencia de estos mecanismos en la distribución actual de las mismas.
La fluorescencia de la clorofila a asociada al fotosistema II se empleó para determinar los parámetros fotosintéticos. Se midieron diversos mecanismos fotoprotectores y antioxidantes (concentración de fenoles, actividad antioxidante mediante el método de DPPH), concentración de clorofilas y carotenoides, así como el contenido interno de C y N, para caracterizar la respuesta fisiológica de ambas especies. Asimismo, se llevaron a cabo experimentos de exposición a altas irradiancias y recuperación en oscuridad para valorar su capacidad de aclimatación.
Se encontraron valores bajos de los parámetros fotosintéticos (tasa de transporte electrónico máximo, eficiencia fotosintética) en C. prolifera. Sin embargo, se observó una mayor capacidad fotosintética y la ausencia de fotoinhibición en C. nodosa, además de una alta concentración de luteína y un alto grado de de-epoxidación correlacionado con un mayor amortiguamiento no fotoquímico.
Los resultados muestran que C. prolifera se comporta como una especie de sombra con una baja capacidad fotoprotectora, siendo la luz uno de los principales factores que determinan su distribución en la laguna. Sin embargo, C. nodosa muestra estar altamente fotoaclimatada a altas irradiancias y su distribución no está directamente relacionada con el ambiente lumínico. Así la regresión de las praderas de Cymodocea, que se observó antes del deterioro de la calidad del agua de la laguna, podría estar relacionada con otros factores ambientales (exceso de materia orgánica, carbonatos en sedimentos, hipoxia…) o con la competencia con C. prolifera. Es necesario realizar experimentos manipulativos para profundizar en el conocimiento de la actual distribución de ambas especies.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
Patrimonio natural antropizado del area minera de Río Tinto (España)
España Ministerio de Educación y Ciencia CGL2008-06270-C y CTM2005-05832 .España Junta de Andalucía P09-RNM-516
Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models
Simple Summary: Mammalian SWI/SNF complexes regulate gene expression by reorganizing the
way DNA is packaged into chromatin. SWI/SNF subunits are recurrently altered in tumors at multiple
levels, including DNA mutations as well as alteration of the levels of RNA and protein. Cancer cell
lines are often used to study SWI/SNF function, but their patterns of SWI/SNF alterations can be
complex. Here, we present a comprehensive characterization of DNA mutations and RNA and protein
expression of SWI/SNF members in 38 lung adenocarcinoma (LUAD) cell lines. We show that over
85% of our cell lines harbored at least one alteration in one SWI/SNF subunit. In addition, over 75% of
our cell lines lacked expression of at least one SWI/SNF subunit at the protein level. Our catalog will
help researchers choose an appropriate cell line model to study SWI/SNF function in LUAD.
Abstract: Mammalian SWI/SNF (SWitch/Sucrose Non-Fermentable) complexes are ATP-dependent
chromatin remodelers whose subunits have emerged among the most frequently mutated genes
in cancer. Studying SWI/SNF function in cancer cell line models has unveiled vulnerabilities
in SWI/SNF-mutant tumors that can lead to the discovery of new therapeutic drugs. However,
choosing an appropriate cancer cell line model for SWI/SNF functional studies can be challenging
because SWI/SNF subunits are frequently altered in cancer by various mechanisms, including genetic
alterations and post-transcriptional mechanisms. In this work, we combined genomic, transcriptomic,
and proteomic approaches to study the mutational status and the expression levels of the SWI/SNF subunits in a panel of 38 lung adenocarcinoma (LUAD) cell lines. We found that the SWI/SNF
complex was mutated in more than 76% of our LUAD cell lines and there was a high variability in the
expression of the di erent SWI/SNF subunits. These results underline the importance of the SWI/SNF
complex as a tumor suppressor in LUAD and the di culties in defining altered and unaltered cell
models for the SWI/SNF complex. These findings will assist researchers in choosing the most suitable
cellular models for their studies of SWI/SNF to bring all of its potential to the development of novel
therapeutic applications.Ministry of Economy of Spain
SAF2015-67919-RJunta de Andalucía
CS2016-3
P12-BIO1655
PIGE-0440-2019
Pl-0245-2017
PI-0135-2020University of Granada
PPJIA2019-0
B-CTS-126-UGR18International Association for the Study of Lung Cancer (IASLC)Spanish Association for Cancer Research (LAB-AECC)PhD "La Caixa Foundation"
LCF/BQ/DE15/10360019"Fundacion Benefica Anticancer Santa Candida y San Francisco Javier" predoctoral fellowshipEuropean Commission
837897Spanish Ministry of Education, Culture and Sports FPU fellowship
FPU17/00067
FPU17/01258
FPU18/03709PhD FPI-fellowship
BES-2013-064596Fundación Científica de la Asociación Española Contra el Cáncer
GCB14-2170Fundación Ramon ArecesInstituto de Salud Carlos III-Fondo de Investigación Sanitaria-Fondo Europeo de Desarrollo Regional `Una manera de hacer Europa' (FEDER)
PI19/0009
Mythos. Mito e imagen en la Antigüedad Clásica y sus pervivencias (IV)
Depto. de Prehistoria, Historia Antigua y ArqueologíaFac. de Geografía e HistoriaFALSEsubmitte
Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling
Estatutos del Partido Andalucista
Estatutos del Partido Andalucista aprobados por el XV Congreso del partido, celebrado en mayo de 2009
Survivin inhibition with YM155 ameliorates experimental pulmonary arterial hypertension
Background: Imbalance between cell proliferation and apoptosis underlies the development of pulmonary arterial hypertension (PAH). Current vasodilator treatment of PAH does not target the uncontrolled proliferative process in pulmonary arteries. Proteins involved in the apoptosis pathway may play a role in PAH and their inhibition might represent a potential therapeutic target. Survivin is a member of the apoptosis inhibitor protein family involved in cell proliferation. Objectives: This study aimed to explore the potential role of survivin in the pathogenesis of PAH and the effects of its inhibition. Methods: In SU5416/hypoxia-induced PAH mice we assessed the expression of survivin by immunohistochemistry, western-blot analysis, and RT-PCR; the expression of proliferation-related genes (Bcl2 and Mki67); and the effects of the survivin inhibitor YM155. In explanted lungs from patients with PAH we assessed the expression of survivin, BCL2 and MKI67. Results: SU5416/hypoxia mice showed increased expression of survivin in pulmonary arteries and lung tissue extract, and upregulation of survivin, Bcl2 and Mki67 genes. Treatment with YM155 reduced right ventricle (RV) systolic pressure, RV thickness, pulmonary vascular remodeling, and the expression of survivin, Bcl2, and Mki67 to values similar to those in control animals. Lungs of patients with PAH also showed increased expression of survivin in pulmonary arteries and lung extract, and also that of BCL2 and MKI67 genes, compared with control lungs. Conclusion: We conclude that survivin might be involved in the pathogenesis of PAH and that its inhibition with YM155 might represent a novel therapeutic approach that warrants further evaluation.The study was supported by grants PI14/0782 and PI17/
1515 from the Instituto de Salud Carlos III (ISCiii), co-funded by the European Union (ERDF/ESF, “A way to make Europe“/
”Investing in your future”), Sociedad Española de Neumología y
Cirugía Torácica (SEPAR), Societat Catalana de Pneumologia
(SOCAP) and Fundación contra la Hipertensión Pulmonar
(FCHP). O. Tura-Ceide is the recipient of a Miguel Servet
contract from the ISCiii (CP17/00114). F. Perez-Vizcaino is
funded by Ministerio de Ciencia e innovación (PID2019-
105847rb-i00), Comunidad de Madrid (CARDIOBOOST/
P2022/BMD-7245)
LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma
Long non-coding RNAs (lncRNAs) are a heterogeneous class of non-coding RNAs whose
biological roles are still poorly understood. LncRNAs serve as gene expression regulators, frequently
interacting with epigenetic factors to shape the outcomes of crucial biological processes, and playing
roles in di erent pathologies including cancer. Over the last years, growing scientific evidence
supports the key role of some lncRNAs in tumor development and proposes them as valuable
biomarkers for the clinic. In this study, we aimed to characterize lncRNAs whose expression is
altered in tumor samples from patients with lung adenocarcinoma (LUAD) compared to adjacent
normal tissue samples. On an RT-qPCR survey of 90 cancer-related lncRNAs, we found one lncRNA,
DLG2-AS1, which was consistently downregulated in 70 LUAD patients. To gain insight into its
biological function, DLG2-AS1 was cloned and successfully re-expressed in LUAD cancer cell lines.
We determined that DLG2-AS1 is not a cis-regulatory element of its overlapping gene DLG2, as their
transcription levels were not correlated, nor did DLG2-AS1 restoration modify the expression of
DLG2 protein. Furthermore, after generating a receiver operating curve (ROC) and calculating the
area under curve (AUC), we found that DLG2-AS1 expression showed high sensitivity and specificity
(AUC = 0.726) for the classification of LUAD and normal samples, determining its value as a potential
lung cancer biomarker.Spanish Ministry of Economy and Business
SAF2015-67919-RJunta de Andalucía
CS2016-3
Pl-0245-2017Asociación Española Contra el Cáncer (AECC) Foundation
LabAECC2018International Association for the Study of Lung Cancer (IASLC)'s Young Investigator Award 2017Spanish Ministry of Education, Culture and Sports FPU fellowship
FPU17/01258
FPU17/00067"Fundacion Benefica Anticancer Santa Candida y San Francisco Javier" predoctoral fellowshipSpanish Ministry of Economy and Business FPI fellowship
BES-2013-064596La Caixa Foundation
LCF/BQ/DE15/10360019Marie Sklodowska Curie Actions postdoctoral fellowship (H2020-MSCA-IF-2018)
83789
Rutas biológicas virtuales por Puerto Real
Se presenta memoria final de proyecto educativo que trata de acercar al alumnado del centro, y por extensión de toda la localidad y la comarca, a una realidad en la que ya vienen trabajando en los diez últimos cursos: la educación medioambiental. Se pretende aprovechar la experiencia y el gran trabajo realizado cursos pasados para editarlos en soportes relacionados con las nuevas tecnologías. Se realiza en el CEIP El Trocadero de Puerto Real, Cádiz y se trata de diseñar una serie de itinerarios ecológicos por las zonas verdes y espacios naturales de la zona de Puerto Real. Los objetivos son: respeto por la naturaleza y del entorno más cercano gracias a su conocimiento; aprendizaje de técnicas de observación directa del entorno inmediato; utilización de las TICs para alumnado y profesorado. El proceso consta de varias fases: visita de itinerarios para la elección de los posibles recursos; elaboración de soportes tanto fotográficos como video; realización de reuniones periódicas para trabajar boceto de presentación; visionado de los diferentes soportes que permanecían en el centro; dar cuerpo a la primera ruta. Los resultados han sido: elaboración y digitalización de todas las fichas de plantas propuestas; se han elaborado, limpiado, seleccionado y digitalizado las distintas láminas y dibujos de animales y plantas de la zona; se confeccionan los distintos planos y rutas a desarrollar para luego digitalizarlos; se recopilaron, clasificaron y ordenaron las distintas imágenes obtenidas en salidas y en material específico del centro; todos los temas tratados se han informatizado.Junta de Andalucía. Consejería de EducaciónAndalucíaES