Pengaruh Technology Acceptance Model Terhadap Pengambilan Keputusan Pembelian Pada Online Shop Grifabell

These days, technology are growing very fast, The use of technology has been widelyused in various sector. For example, in the fashion sector, also using technology asa medium to promote and to market the product. And with the increasing numberof Internet users, are expected to influence people's behavior to make a purchasefrom conventional purchases to e-commerce.To realize a transaction or customerparticipation, it takes trust of each customer. In this study, the population is fromthe respondents who have ever made a purchase of shoes from Grifabell throughsocial media instagram. Data collection techniques used is through a survey using aquestionnaire, and questionnaires collected from a sample of 120 respondents whichhas been distributed through an Google Drive's online questionnaire to Grifabell'scustomers throughout Indonesia. Before the data were analyzed, the authors testedthe validity and reliability of 30 samples using SPSS software.The research method used is multiple regression analysis and simple regression, Ftest, T test and determination coefficient. From these results, it can be concludedthat the indicators of variable technology acceptance model: the perceived qualityof e-shopping and attitude have a positive effect on the variable of trust, andthe indicators from confidence's variables is experience, the quality of work, andintelligence which have a positive effect on the purchase decision variables

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Elevated Activation of Neutrophil Toll-Like Receptors in Patients with Acute Severe Leptospirosis: An Observational Study

Leptospirosis is the leading cause of zoonotic morbidity and mortality globally, yet little is known about the immune mechanisms that may contribute to pathogenesis and severe disease. While neutrophils are a key component of early immune responses to infection, they have been associated with tissue damage and inflammation in some febrile infections. To assess whether neutrophils contribute to the pathogenesis observed in severe leptospirosis, we quantitated levels of neutrophil activation markers in patients with varying disease severities. Hospitalized leptospirosis patients had significantly higher levels of TLR2 and TLR4 on peripheral neutrophils compared to healthy controls, with the highest levels detected in patients with organ dysfunction. We observed no significant differences in other neutrophil baseline activation markers (CD62L and CD11b) or activation capacity (CD62L and CD11b levels following stimulation) regardless of disease severity. Our results provide preliminary evidence supporting the hypothesis that higher initial bacterial loads or inadequate or delayed neutrophil responses, rather than TLR-driven inflammation, may drive severe disease outcomes.National Institute of Allergy and Infectious Diseases at the National Institutes of Health, United States Department of Health & Human Services, National Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [U01AI088752, AI 089992, AG 042489]; American Society for Tropical Medicine and Hygiene Gorgas Memorial Institute Research Award; Fogarty International Center, Global Health Equity Scholars Fellowship [R25 TW009338]; Fundacao Oswaldo Cruz/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-Ciencia Sem Fronteiras Bolsa Jovens Talentos12 month embargo; published online 22 July 2019This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Correction: Cathelicidin Insufficiency in Patients with Fatal Leptospirosis.

[This corrects the article DOI: 10.1371/journal.ppat.1005943.]

Cathelicidin Insufficiency in Patients with Fatal Leptospirosis

<div><p>Leptospirosis causes significant morbidity and mortality worldwide; however, the role of the host immune response in disease progression and high case fatality (>10–50%) is poorly understood. We conducted a multi-parameter investigation of patients with acute leptospirosis to identify mechanisms associated with case fatality. Whole blood transcriptional profiling of 16 hospitalized Brazilian patients with acute leptospirosis (13 survivors, 3 deceased) revealed fatal cases had lower expression of the antimicrobial peptide, cathelicidin, and chemokines, but more abundant pro-inflammatory cytokine receptors. In contrast, survivors generated strong adaptive immune signatures, including transcripts relevant to antigen presentation and immunoglobulin production. In an independent cohort (23 survivors, 22 deceased), fatal cases had higher bacterial loads (<i>P</i> = 0.0004) and lower anti-<i>Leptospira</i> antibody titers (<i>P</i> = 0.02) at the time of hospitalization, independent of the duration of illness. Low serum cathelicidin and RANTES levels during acute illness were independent risk factors for higher bacterial loads (<i>P</i> = 0.005) and death (<i>P</i> = 0.04), respectively. To investigate the mechanism of cathelicidin in patients surviving acute disease, we administered LL-37, the active peptide of cathelicidin, in a hamster model of lethal leptospirosis and found it significantly decreased bacterial loads and increased survival. Our findings indicate that the host immune response plays a central role in severe leptospirosis disease progression. While drawn from a limited study size, significant conclusions include that poor clinical outcomes are associated with high systemic bacterial loads, and a decreased antibody response. Furthermore, our data identified a key role for the antimicrobial peptide, cathelicidin, in mounting an effective bactericidal response against the pathogen, which represents a valuable new therapeutic approach for leptospirosis.</p></div