361 research outputs found

    Crystal structure of the high temperature phase of strontium barium niobate

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    Pure and undoped strontium-barium niobate Sr0:40Ba0:60Nb2O6 (SBN40) single crystals grown by the Czochralski method were investigated by single crystal X-ray diffraction methods. The study below TC (429 K for SBN40) confirmed the structure with P4bm space group. Above this temperature the structure transforms into the paraelectric, centrosymmetric one with P4=mbm space group. Analysis of the recorded diffraction patterns allowed to observe several signs of crystal structure modulation. On the registered diffraction images satellite reflections were found. A modulation vector q = ( ; ; ), where = 0:3075(6) (at room temperature) was found and it was similar to that occurring in the SBN61. In addition, above the phase transition temperature on the (hk) planes with l integer a weak diffuse scattering was observed

    Quantifying community resilience to riverine hazards in Bangladesh

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    Every year, 30–70% of Bangladesh is inundated with flood waters, which combined with erosion, affect between 10 and 70 million people annually. Rural riverine communities in Bangladesh have long been identified as some of the poorest populations, most vulnerable to riverine hazards. However, these communities have, for generations, also developed resilience strategies – considered as the combination of absorptive, adaptive, and transformative approaches – to manage significant flooding and erosion. It is not clear whether such existing strategies are sufficient to generate resilience in the face of increasing hazards and growing pressures for land. In this study, we quantify community resilience to flooding and erosion of 35 of the most poverty-stricken and exposed communities in riverine Bangladesh by applying the systematic resilience measurement framework provided by the Flood Resilience Measurement for Communities tool. The low levels of resilience observed in the riverine communities, as well as their continued focus on enhancing absorptive capacities are alarming, especially in the face of growing climate threats and continued population growth. Innovative transformative responses are urgently required in riverine Bangladesh, which align with and complement ongoing community-centred efforts to enhance rural resilience to riverine hazards

    Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences

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    Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications that the tomato DNA inserts conferred autonomous replication on the plasmids. Instead, Southern blot hybridization analysis of seven kanamycin resistant calli revealed the presence of at least one kanamycin resistance locus per transformant integrated in the tomato nuclear DNA. Generally one to three truncated plasmid copies were found integrated into the tomato nuclear DNA, often physically linked to each other. For one transformant we have been able to use the bacterial ampicillin resistance marker of the vector plasmid pUC9 to 'rescue' a recombinant plasmid from the tomato genome. Analysis of the foreign sequences included in the rescued plasmid showed that integration had occurred in a non-repetitive DNA region. Calf-thymus DNA, used as a carrier in transformation procedure, was found to be covalently linked to plasmid DNA sequences in the genomic DNA of one transformant. A model is presented describing the fate of exogenously added DNA during the transformation of a plant cell. The results are discussed in reference to the possibility of isolating DNA sequences responsible for autonomous replication in tomato.

    Acceleration of generalized hypergeometric functions through precise remainder asymptotics

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    We express the asymptotics of the remainders of the partial sums {s_n} of the generalized hypergeometric function q+1_F_q through an inverse power series z^n n^l \sum_k c_k/n^k, where the exponent l and the asymptotic coefficients {c_k} may be recursively computed to any desired order from the hypergeometric parameters and argument. From this we derive a new series acceleration technique that can be applied to any such function, even with complex parameters and at the branch point z=1. For moderate parameters (up to approximately ten) a C implementation at fixed precision is very effective at computing these functions; for larger parameters an implementation in higher than machine precision would be needed. Even for larger parameters, however, our C implementation is able to correctly determine whether or not it has converged; and when it converges, its estimate of its error is accurate.Comment: 36 pages, 6 figures, LaTeX2e. Fixed sign error in Eq. (2.28), added several references, added comparison to other methods, and added discussion of recursion stabilit

    Electric field and aging effects of uniaxial ferroelectrics Sr x Ba1−x Nb2O6 probed by Brillouin scattering

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    Static and dynamic heterogeneity of disordered system is one of the current topics in materials science. In disordered ferroelectric materials with random fields, dynamic polar nanoregions (PNRs) appear at Burns temperature and freeze into nanodomain state below Curie temperature (TC). This state is very sensitive to external electric field and aging by which it gradually switches into macrodomain state. However, the role of PNRs in such states below TC is still a puzzling issue of materials science. Electric field and aging effects of uniaxial ferroelectric Sr x Ba1−x Nb2O6 (x = 0.40, SBN40) single crystals were studied using Brillouin scattering to clarify the critical nature of PNRs in domain states below TC. On field heating, a broad anomaly in longitudinal acoustic (LA) velocity at low temperature region was due to an incomplete alignment of nanodomains caused by the interaction between PNRs. A sharp anomaly near TC was attributed to the complete switching of nanodomain to macrodomain state owing to the lack of interaction among PNRs. After isothermal aging below TC, the noticeable increase of LA velocity was observed. It was unaffected by cyclic temperature measurements up to TC, and recovered to initial state outside of a narrow temperature range above and below aging temperature

    Comparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division

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    Proliferation of mammalian cells requires the coordinated function of many proteins to accurately divide a cell into two daughter cells. Several RNAi screens have identified previously uncharacterised genes that are implicated in mammalian cell division. The molecular function for these genes needs to be investigated to place them into pathways. Phenotypic profiling is a useful method to assign putative functions to uncharacterised genes. Here, we show that the analysis of protein localisation is useful to refine a phenotypic profile. We show the utility of this approach by defining a function of the previously uncharacterised gene C13orf3 during cell division. C13orf3 localises to centrosomes, the mitotic spindle, kinetochores, spindle midzone, and the cleavage furrow during cell division and is specifically phosphorylated during mitosis. Furthermore, C13orf3 is required for centrosome integrity and anaphase onset. Depletion by RNAi leads to mitotic arrest in metaphase with an activation of the spindle assembly checkpoint and loss of sister chromatid cohesion. Proteomic analyses identify C13orf3 (Ska3) as a new component of the Ska complex and show a direct interaction with a regulatory subunit of the protein phosphatase PP2A. All together, these data identify C13orf3 as an important factor for metaphase to anaphase progression and highlight the potential of combined RNAi screening and protein localisation analyses
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