Chiapello, M

Funaya, C

Hillmer, S

Kahmann, R

Lo Presti, L

Paszkowski, U

Roth, R

Schumacher, K

English

During establishment of arbuscular mycorrhizal symbioses, fungal hyphae invade root cells producing transient tree-like structures, the arbuscules, where exchange of photosynthates for soil minerals occurs. Arbuscule formation and collapse lead to rapid production and degradation of plant and fungal membranes, their spatiotemporal dynamics directly influencing nutrient exchange. We determined the ultra-structural details of both membrane surfaces and the interstitial apoplastic matrix by transmission electron microscopy tomography during growth and senescence of Rhizophagus irregularis arbuscules in rice. Invasive growth of arbuscular hyphae was associated with abundant fungal membrane tubules (memtubs) and plant peri-arbuscular membrane evaginations. Similarly, the phylogenetically distant arbuscular mycorrhizal fungus, Gigaspora rosea, and the fungal maize pathogen, Ustilago maydis, developed memtubs while invading host cells, revealing structural commonalities independent of the mutualistic or parasitic outcome of the interaction. Additionally, extracellular vesicles formed continuously in the peri-arbuscular interface from arbuscule biogenesis to senescence, suggesting an involvement in inter-organismic signal and nutrient exchange throughout the arbuscule lifespan

Roth, R.

Hillmer, S.

Funaya, C.

Chiapello, M.

Schumacher, K.

Lo Presti, L.

Kahmann, R.

Paszkowski, U.

MPG.PuRe

Arbuscular cell invasion coincides with extracellular vesicles and membrane tubules

Apollo (Cambridge)

Roth, Ronelle

Oxford University Research Archive

During establishment of arbuscular mycorrhizal symbioses, fungal hyphae invade root cells producing transient tree-like structures, the arbuscules, where exchange of photosynthates for soil minerals occurs. Arbuscule formation and collapse lead to rapid production and degradation of plant and fungal membranes, their spatiotemporal dynamics directly influencing nutrient exchange. We determined the ultra-structural details of both membrane surfaces and the interstitial apoplastic matrix by transmission electron microscopy tomography during growth and senescence of Rhizophagus irregularis arbuscules in rice. Invasive growth of arbuscular hyphae was associated with abundant fungal membrane tubules (memtubs) and plant peri-arbuscular membrane evaginations. Similarly, the phylogenetically distant arbuscular mycorrhizal fungus, Gigaspora rosea, and the fungal maize pathogen, Ustilago maydis, developed memtubs while invading host cells, revealing structural commonalities independent of the mutualistic or parasitic outcome of the interaction. Additionally, extracellular vesicles formed continuously in the peri-arbuscular interface from arbuscule biogenesis to senescence, suggesting an involvement in inter-organismic signal and nutrient exchange throughout the arbuscule lifespan.R. Roth was supported by Marie Curie FP7-PEOPLE-2013-IEF grant No. 629887 and by the Isaac Newton Trust RG74108; and U. Paszkowski by the BBSRC grant No. BB/N008723/1

Hillmer, Stefan

Funaya, Charlotta

Chiapello, Marco

Schumacher, Karin

Lo Presti, Libera

Kahmann, Regine

Paszkowski, Uta

Arbuscular cell invasion coincides with extracellular vesicles and membrane tubules.

ORA - Oxford University Research Archive

https://www.repository.cam.ac.uk/bitstream/1810/305876/5/Roth%20et%20al.%202020%20Nat%20Plants_accepted.pdf

Arbuscular cell invasion coincides with extracellular vesicles and membrane tubules

Abstract

Similar works

Full text

Available Versions

MPG.PuRe

Apollo (Cambridge)

Oxford University Research Archive

Apollo (Cambridge)

ORA - Oxford University Research Archive