Early Western Mediterranean Impressed Wares (Early 6th millennium cal BCE), sourcing, production, uses and transfers: design of new interdisciplinary research and first results at Pendimoun rock-shelter (Alpes-Maritimes, France)

International audienceEarly Western Mediterranean Impressed Wares (Early 6th millennium cal BCE), sourcing, production, uses and transfers: design of new interdisciplinary research and first results at Pendimoun rock-shelter (Alpes-Maritimes, France

Early Western Mediterranean Impressed Wares (Early 6th millennium cal BCE), sourcing, production, uses and transfers: design of new interdisciplinary research and first results at Pendimoun rock-shelter (Alpes-Maritimes, France)

International audienceEarly Western Mediterranean Impressed Wares (Early 6th millennium cal BCE), sourcing, production, uses and transfers: design of new interdisciplinary research and first results at Pendimoun rock-shelter (Alpes-Maritimes, France

Autour de Pendimoun : une approche systémique de la production céramique

International audienc

Autour de Pendimoun : une approche systémique de la production céramique

International audienc

A refined genome phage display methodology delineates the human antibody response in patients with Chagas disease

Summary: Large-scale mapping of antigens and epitopes is pivotal for developing immunotherapies but challenging, especially for eukaryotic pathogens, owing to their large genomes. Here, we developed an integrated platform for genome phage display (gPhage) to show that unbiased libraries of the eukaryotic parasite Trypanosoma cruzi enable the identification of thousands of antigens recognized by serum samples from patients with Chagas disease. Because most of these antigens are hypothetical proteins, gPhage provides evidence of their expression during infection. We built and validated a comprehensive map of Chagas disease antibody response to show how linear and putative conformation epitopes, many rich in repetitive elements, allow the parasite to evade a buildup of neutralizing antibodies directed against protein domains that mediate infection pathogenesis. Thus, the gPhage platform is a reproducible and effective tool for rapid simultaneous identification of epitopes and antigens, not only in Chagas disease but perhaps also in globally emerging/reemerging acute pathogens