Turnover of outer and inner membrane proteins of rat liver mitochondria

Recent reports on the properties and composition of outer and inner mitochondrial membranes suggest that outer and inner membranes of mitochondria are derived from different sources of the cell [l-3]. I

Doing It Ourselves: The Networked Practices Of Feminist Media Activism

Feminist organizing in the United States is undergoing a paradigm shift. Whereas 1960s-era feminism unfolded through the in-person activities of formal organizations, today, feminist movements are mediated and networked. Contemporary feminism manifests as hashtags, blogs, print zines, digitally coordinated protests, online communities, and more. Case studies of recent movements suggest that, for feminists, to be networked is to be both politically empowered and politically vulnerable. At the same time that emerging media platforms enable activists to quickly reach wide audiences at little or no expense, networked movements face online harassment, commercial cooptation, and activist burnout. This qualitative study examines how feminists are navigating the double-edged nature of networked activism. In particular, I demonstrate how feminists are drawing on networked media to organize resistance, mobilize protests, and cultivate communities, all while juggling the affordances and limitations of their media tools. Data for this study come from an ethnographic analysis of grassroots feminist media activism in the city of Philadelphia, textual analyses of national and transnational feminist media campaigns, and interviews with and archived reflections from activists. Through this data, I argue that feminists’ negotiations among media platforms, the political context, and their intersectional values produce a particular activist praxis. I call this praxis do-it-ourselves (DIO) feminism, an organizing paradigm that draws on networked media to build feminist movements from the ground up that reflect feminist values and meet the challenges of the current climate. Faced with an electoral system and a history of collective organizing that has failed to address intersecting systems of oppression, DIO feminists do not rely on existing political institutions. Instead, they draw on networked media to create their own communities, discourses, and protests, cutting out the middlemen of political leaders, organizations, parties, and policy-makers and integrating protest into everyday life. Feminists’ turn toward diffuse, decentralized media networks and away from formal organizations raises questions about movement accessibility, sustainability, backlash, impact, and cooptation. But the do-it-ourselves praxis is a distinctly feminist form of networked activism that models new possibilities for what collective action and social transformation can look like in the networked era

All Transients, All the Time: Real-Time Radio Transient Detection with Interferometric Closure Quantities

We demonstrate a new technique for detecting radio transients based on interferometric closure quantities. The technique uses the bispectrum, the product of visibilities around a closed-loop of baselines of an interferometer. The bispectrum is calibration independent, resistant to interference, and computationally efficient, so it can be built into correlators for real-time transient detection. Our technique could find celestial transients anywhere in the field of view and localize them to arcsecond precision. At the Karl G. Jansky Very Large Array (VLA), such a system would have a high survey speed and a 5-sigma sensitivity of 38 mJy on 10 ms timescales with 1 GHz of bandwidth. The ability to localize dispersed millisecond pulses to arcsecond precision in large volumes of interferometer data has several unique science applications. Localizing individual pulses from Galactic pulsars will help find X-ray counterparts that define their physical properties, while finding host galaxies of extragalactic transients will measure the electron density of the intergalactic medium with a single dispersed pulse. Exoplanets and active stars have distinct millisecond variability that can be used to identify them and probe their magnetospheres. We use millisecond time scale visibilities from the Allen Telescope Array (ATA) and VLA to show that the bispectrum can detect dispersed pulses and reject local interference. The computational and data efficiency of the bispectrum will help find transients on a range of time scales with next-generation radio interferometers.Comment: Accepted to ApJ. 8 pages, 5 figures, 2 tables. Revised to include discussion of non-Gaussian statistics of techniqu

Temporal variations in English Populations of a forest insect pest, the green spruce aphid (Elatobium abietinum), associated with the North Atlantic Oscillation and global warming

Based on an exceptionally long modern ecological dataset (41 years), it has been possible to show that warm weather in England associated with a positive North Atlantic Oscillation (NAO) index causes the spring migration of the green spruce aphid (Elatobium abietinum), a pest species of spruce trees (Picea) to start earlier, continue for longer and contain more aphids. An upward trend in the NAO index during the period 1966-2006 is associated with an increasing population size of E. abietinum. It is important to understand the mechanisms behind the population fluctuations, because this aphid causes considerable damage to Picea plantations. Present day weather associated fluctuations in forest insect pests may be useful analogues in understanding past pest outbreaks in forests

Prevalence of blindness in Western Australia: a population study using capture and recapture techniques

Aim: To determine the prevalence of blinding eye disease in Western Australia using a capture and recapture methodology. Methods: Three independent lists of residents of Western Australia who were also legally blind were collated during the capture periods in 2008–9. The first list was obtained from the state-wide blind register. A second list comprised patients routinely attending hospital outpatient eye clinics over a 6-month period in 2008. The third list was patients attending ophthalmologists' routine clinical appointments over a 6-week period in 2009. Lists were compared to identify those individuals who were captured on each list and those who were recaptured by subsequent lists. Log-linear models were used to calculate the best fit and estimate the prevalence of blindness in the Western Australian population and extrapolated to a national prevalence of blindness in Australia. Results: 1771 legally blind people were identified on three separate lists. The best estimate of the prevalence of blindness in Western Australia was 3384 (95% CI 2947 to 3983) or 0.15% of the population of 2.25 million. Extrapolating to the national population (21.87 million) gave a prevalence of legal blindness of approximately 32 892 or 0.15%. Conclusion: Capture–recapture techniques can be used to determine the prevalence of blindness in whole populations. The calculated prevalence of blindness suggested that up to 30% of legally blind people may not be receiving available financial support and up to 60% were not accessing rehabilitation services

The politics of developing reference standards for nutrient intakes : the case of Australia and New Zealand

Objective Nutrient Reference Values (NRV) are evidence-based benchmarks for assessing the dietary adequacy of individuals and population groups as well as informing public health nutrition policies and programmes. The present paper presents the findings of an analysis of the views of submitters to a draft document associated with the development of the 2006 NRV for Australia and New Zealand. The aim of the study was to explore how these views were reflected in the policy-making process and final policy document.Design The information necessary to fulfil this aim required access to stakeholder submissions to the NRV development process and this necessitated exploiting the provisions of the Commonwealth of Australia&rsquo;s Freedom of Information (FOI) Act 1982. We understand that the present research represents the first time that an FOI request seeking information about a National Health and Medical Research Council food and nutrition policy process has been made and therefore is novel in its approach to public health nutrition policy analysis.Results The analysis of stakeholder submissions identified that stakeholders had particular concerns about the conduct of the review process and the future application of the nutrient values to policy and programmes. There is a lack of evidence that the majority of stakeholder comments were addressed in the final NRV document.Conclusion Although these findings cannot be interpreted to assess the validity or otherwise of the set nutrient values, they do raise questions about the process for their development and the adequacy of the final document to reflect the views of key stakeholders.<br /

Lipid production through the single-step microwave hydrolysis of macroalgae using the oleaginous yeast Metschnikowia pulcherrima

Macroalgae (seaweeds) represent an emerging resource for food and the production of commodity and specialty chemicals. In this study, a single-step microwave process was used to depolymerise a range of macroalgae native to the United Kingdom, producing a growth medium suitable for microbial fermentation. The medium contained a range of mono- and polysaccharides as well as macro- and micronutrients that could be metabolised by the oleaginous yeast Metschnikowia pulcherrima. Among twelve macroalgae species, the brown seaweeds exhibited the highest fermentation potential, especially the kelp Saccharina latissima. Applying a portfolio of ten native M. pulcherrima strains, yeast growth kinetics, as well as production of lipids and 2-phenylethanol were examined, with productivity and growth rate being strain dependent. On the 2 L scale, 6.9 g L−1 yeast biomass – a yield of 0.14 g g−1 with respect to the supplied macroalgae – containing 37.2% (w/w) lipid was achieved through utilisation of the proteins, mono- and polysaccharides from S. latissima, with no additional enzymes. In addition, the yeast degraded a range of fermentation inhibitors released upon microwave processing at high temperatures and long holding times. As macroalgae can be cultured to food grade, this system offers a novel, potentially low-cost route to edible microbial oils as well as a renewable feedstock for oleochemicals

The effect of efferentiectomy and orchidectomy on the metabolism of the epididymis of the mouse

The purpose of the study is a comparison of respiratory metabolic rates and anaerobic glycolytic activity (lactate accumulation) in homogenates of epididymides from normal, orchidectomized and testosterone replaced-orchidectomized mice. Animals subjected to vasa efferentiectomy were used to furnish sperm free homogenates as controls for the homogenates from orchidectomized animals, and to furnish estimates of the contribution of the spermatozoa to these metabolic parameters in homogenates of normal epididymides. Efferentiectomized mice yield homogenates with a significantly lowered rate of respiratory activity (30%). Experiments with fractions of these homogenates showed this reduction in activity to be equivalent to the contribution of the spermatozoa in homogenates from normal mice. No significant difference in the rate of respiratory metabolism was observed among homogenates obtained from efferentiectomized, orchidectomized, testosterone replaced-orchidectomized, or testosterone injected-efferentiectomized mice. Efferentiectomy also resulted in a lowered rate of anaerobic glycolytic activity in vitro . No further reduction or elevation of this activity was observed in homogenates from orchidectomized or testosterone injected animals. Further study indicated this loss following efferentiectomy to be due to a reduced glycolytic activity in the epididymal epithelium rather than to the absence of spermatozoan contribution to the metabolism. The evidence presented supports the hypothesis that the presence of some component of the spermatozoa or the fluids in which they reside controls the activation or inhibition of a segment of the lactic dehydrogenase activity which is responsible for the secretion of lactic acid into the epididymal canal for spermatozoan utilization.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50126/1/1030660306_ftp.pd

Non-song vocalizations of pygmy blue whales in Geographe Bay, Western Australia.

Non-song vocalizations of migrating pygmy blue whales (Balaenoptera musculus brevicauda) in Western Australia are described. Simultaneous land-based visual observations and underwater acoustic recordings detected 27 groups in Geographe Bay, WA over 2011 to 2012. Six different vocalizations were recorded that were not repeated in a pattern or in association with song, and thus were identified as non-song vocalizations. Five of these were not previously described for this population. Their acoustic characteristics and context are presented. Given that 56% of groups vocalized, 86% of which produced non-song vocalizations and 14% song units, the inclusion of non-song vocalizations in passive-acoustic monitoring is proposed