2013 National Turfgrass Evaluation Program Bermudagrass Test: 2016 Data

Kansas represents the northernmost region in the central United States where bermudagrass can be successfully grown as a perennial turfgrass. Historically, few cultivars that have both acceptable quality and adequate cold tolerance have been available to local growers. Because new introductions are continually being selected for improved hardiness and quality, both seeded and vegetative types need regular evaluation to determine their long-range suitability for use in Kansas

Polymorphisms in the circadian expressed genes PER3 and ARNTL2 are associated with diurnal preference and GNβ3 with sleep measures

Sleep and circadian rhythms are intrinsically linked, with several sleep traits, including sleep timing and duration, influenced by both sleep homeostasis and the circadian phase. Genetic variation in several circadian genes has been associated with diurnal preference (preference in timing of sleep), although there has been limited research on whether they are associated with other sleep measurements. We investigated whether these genetic variations were associated with diurnal preference (Morningness-Eveningness Questionnaire) and various sleep measures, including: the global Pittsburgh Sleep Quality index score; sleep duration; and sleep latency and sleep quality. We genotyped 10 polymorphisms in genes with circadian expression in participants from the G1219 sample (n = 966), a British longitudinal population sample of young adults. We conducted linear regressions using dominant, additive and recessive models of inheritance to test for associations between these polymorphisms and the sleep measures. We found a significant association between diurnal preference and a polymorphism in period homologue 3 (PER3) (P < 0.005, recessive model) and a novel nominally significant association between diurnal preference and a polymorphism in aryl hydrocarbon receptor nuclear translocator-like 2 (ARNTL2) (P < 0.05, additive model). We found that a polymorphism in guanine nucleotide binding protein beta 3 (GNβ3) was associated significantly with global sleep quality (P < 0.005, recessive model), and that a rare polymorphism in period homologue 2 (PER2) was associated significantly with both sleep duration and quality (P < 0.0005, recessive model). These findings suggest that genes with circadian expression may play a role in regulating both the circadian clock and sleep homeostasis, and highlight the importance of further studies aimed at dissecting the specific roles that circadian genes play in these two interrelated but unique behaviours

Salinity History of Coastal Marshes Reconstructed from Diatom Remains

Sediment cores were collected from three Louisiana coastal marsh ponds, dated with radioisotopes, and analyzed for diatom remains to determine if long-term salinity changes were evident in the sediment record. A diatom-based salinity index formulated from a statistical comparison of available salinity data and changing diatom assemblages demonstrated that diatom remains appear to preserve salinity signals in coastal brackish and salt marsh environments. The salinity index was applied to sediment cores spanning the late 1600s to the 1990s and provided a more complete record of salinity than field data, which were temporally and spatially incomplete. The salinity reconstructions indicated that salinity has increased at two sites and decreased at a third since the early 1900s. The salinity changes are less than 1‰ per decade in all cases, and may be due to natural variability as depicted by the wide range of salinities observed between the late 1600s and 1900. Salinity regimes may be very localized (\u3c2 km from a hydrologic source), indicating single-site studies may not be applicable to regional inferences. This study demonstrates that diatoms can be used to reconstruct past salinity in coastal marsh environments and can provide a useful tool with which to study the changing hydrology of river-influenced ecosystems

2012 National Turfgrass Evaluation Program Tall Fescue Test: 2016 Data

Research efforts to improve cultivar quality include selecting for stress tolerance and disease resistance as well as finer leaf texture, a rich green color, and better sward density. Several cultivars included in the 2012 National Turfgrass Evaluation Program Tall Fescue Test performed well and showed good brown patch resistance in south central Kansas during the 2016 growing season

Assessing the use of artificial substrates to monitor Gambierdiscus populations in the Florida Keys

© The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Harmful Algae 68 (2017): 52-66, doi:10.1016/j.hal.2017.07.007.Four distinct coastal locations were sampled on a monthly basis near Long Key (Florida Keys, USA) over a 13-month period to study Gambierdiscus population dynamics on different substrates, including four macrophyte species (Dictyota spp., Halimeda spp., Laurencia spp., and Thalassia testudinum) and three artificial substrates (polyvinyl chloride (PVC) tiles, burlap, and fiberglass window screen). Cell densities of Gambierdiscus were generally lower on Dictyota versus Halimeda and Laurencia. Cell densities of Gambierdiscus were significantly correlated among macrophyte hosts in 54% of the comparisons, and between macrophyte hosts and artificial substrates in 72% of the comparisons. Predictive slopes determined from regression analyses between cell densities on artificial substrates and macrophyte hosts indicated that, on an areal basis, fewer cells were present on macrophytes versus artificial substrates (cells cm-2) and that slope variation (error) among the different macrophytes and sites ranged from 5% to 200%, averaging 61% overall. As the data required log-transformation prior to analyses, this level of error translates into two-orders of magnitude in range of estimation of the overall average abundance of Gambierdiscus cells on macrophytes (135 cells g-1 wet weight); 20 to 2690 cells g-1 ww. The lack of consistent correlation among Gambierdiscus cell densities on macrophytes versus artificial substrates, coupled with the high level of error associated with the predictive slope estimations, indicates that extreme caution should be taken when interpreting the data garnered from artificial substrate deployments, and that such deployments should be thoroughly vetted prior to routine use for monitoring purposes.Funding for this work was provided by NOAA NOS (Cooperative Agreements NA11NOS478-0060 and NA11NOS4780028)

Influence of a Cyclonic Eddy on Microheterotroph Biomass and Carbon Export in the Lee of Hawaii

[1] A multi‐platform sampling strategy was used to investigate carbon cycling in a cold‐core eddy that formed in the lee of Hawaii during September 2000. Microheterotroph biomass and 234Th‐derived carbon export rates within the eddy were 2 to 3 times higher than those observed for adjacent waters. If this eddy is representative of other cyclonic eddies that are frequently formed in the lee of Hawaii, then eddy activity may significantly enhance the areal efficiency of the biological pump and facilitate the transfer of organic carbon to organisms inhabiting the mesopelagic and abyssal‐benthic zones of this subtropical ecosystem

Mathematical modelling of fibre-enhanced perfusion inside\ud a tissue-engineering bioreactor

We develop a simple mathematical model for forced flow of culture medium through a porous scaffold in a tissue- engineering bioreactor. Porous-walled hollow fibres penetrate the scaffold and act as additional sources of culture medium. The model, based on Darcy’s law, is used to examine the nutrient and shear-stress distributions throughout the scaffold. We consider several configurations of fibres and inlet and outlet pipes. Compared with a numerical solution of the full Navier–Stokes equations within the complex scaffold geometry, the modelling approach is cheap, and does not require knowledge of the detailed microstructure of the particular scaffold being used. The potential of this approach is demonstrated through quantification of the effect the additional flow from the fibres has on the nutrient and shear-stress distribution

Pennsylvania Folklife Vol. 25, No. 3

• The Pennsylvania Germans and the American Revolution • The Blooming Grove Colony • The Salebill • The Schlegel Family and the Rosicrucian Movement • A Log Settler\u27s Fort/Home • Pennsylvania Dutch Studies at Ursinus College, 1976 • The Country Sale: Folk-Cultural Questionnaire No. 43https://digitalcommons.ursinus.edu/pafolklifemag/1067/thumbnail.jp

Immunolocalization and temporal distribution of cytokine expression during the development of vein graft intimal hyperplasia in an experimental model

AbstractPurpose: Vein graft stenosis caused by intimal hyperplasia (IH) accounts for 30% to 50% of late bypass graft failures; however, the biochemical mediators of vein graft IH have been poorly defined. We attempted to evaluate the spatial and temporal distribution of five principal cytokines (interleukin-1 beta [IL-1β], platelet-derived growth factor AA [PDGF-AA], basic fibroblast growth factor [bFGF], interferon gamma [INFγ], and tumor necrosis factor alpha [TNF-α]) during the development of IH in a rat vein graft model.Methods: Rat epigastric vein interposition grafts in the femoral artery were harvested at 6 hours, 2 days, 1 week, 2 weeks, and 4 weeks after the grafting procedure and studied with immunohistochemical and standard histologic techniques. The cytokine expression in the endothelium and media/neointima was quantified as the percentage of immunopositive cells per high-power field.Results: Maximal hyperplasia occurred 2 weeks after the grafting procedure. Peak expression of IL-1β and bFGF occurred by 2 days. PDGF-AA expression paralleled the development of IH, peaking at 2 weeks and then declining. TNF-α expression increased at 1 week and remained elevated. INFγ was seen only in control grafts.Conclusions: The coordinated early release of IL-1β and bFGF and the down-regulation of INFγ seem to trigger an inflammatory response, thereby initiating IH. The process then is propagated by the release of PDGF-AA and TNF-α, with concomitant smooth muscle cell proliferation and production of extracellular matrix. It is likely that this complex milieu of local paracrine signaling is required to generate the hyperplastic response seen in failing vein grafts. (J Vasc Surg 1996;24:463-71.

Cast versus functional brace in the rehabilitation of patients treated non-operatively for a rupture of the Achilles tendon: protocol for the UK study of tendo achilles rehabilitation (UK STAR) multi-centre randomised trial

INTRODUCTION Achilles tendon rupture affects over 11 000 people yearly in the UK, and the incidence is increasing. Controversy remains with regard to the best rehabilitation strategy for these patients. In operatively treated patients, functional bracing provides better outcomes compared with plaster casts. However, the role of functional bracing in non-operatively managed patients is unclear. This is the protocol for a multicentre randomised trial of plaster cast immobilisation versus functional bracing for patients with a non-operatively managed Achilles tendon rupture. METHODS AND ANALYSIS All adults presenting with a primary rupture of the Achilles tendon will be screened. Non-operatively treated patients will be eligible to take part in the trial. Broad eligibility criteria will ensure that the results of the study can be generalised to the wider patient population. Randomisation will be on a 1:1 basis. Both rehabilitation strategies are widely used within the National Health Service. Standardised protocols will be followed, and details of plaster material and brace will be as per the site's usual practice.A minimum of 330 patients will be randomised to obtain 90% power to detect a difference of 8 points in Achilles Tendon Total Rupture Score at 9 months. Quality of life and resource use will be collected at 3, 6 and 9 months. The differences between treatment groups will be assessed on an intention-to-treat basis. The results of the trial-based economic evaluation will be expressed in terms of incremental cost per quality-adjusted life-year gained. ETHICS AND DISSEMINATION The National Research Ethic Committee approved this study on 18 March 2016 (16/SC/0109).The National Institute for Health Research Health Technology Assessment monograph and a manuscript to a peer-reviewed journal will be submitted on completion of the trial (summer 2019). The results of this trial will substantially inform clinical practice on the clinical and cost-effectiveness of the treatment of this injury. This study has been registered on the International Standard Randomised Controlled Trial Number registry with reference no ISRCTN62639639