Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement

Compartmentalization is an important process, since it allows the segregation of metabolic activities and, in the era of synthetic biology, represents an important tool by which defined microenvironments can be created for specific metabolic functions. Indeed, some bacteria make specialized proteinaceous metabolic compartments called bacterial microcompartments (BMCs) or metabolosomes. Here we demonstrate that the shell of the metabolosome (representing an empty BMC) can be produced within E. coil cells by the coordinated expression of genes encoding structural proteins. A plethora of diverse structures can be generated by changing the expression profile of these genes, including the formation of large axial filaments that interfere with septation. Fusing GFP to PduC, PduD, or PduV, none of which are shell proteins, allows regiospecific targeting of the reporter group to the empty BMC. Live cell imaging provides unexpected evidence of filament-associated BMC movement within the cell in the presence of Pdu

A pulsating white dwarf in an eclipsing binary

White dwarfs are the burnt-out cores of Sun-like stars and are the fate of 97 per cent of the stars in our Galaxy. The internal structure and composition of white dwarfs are hidden by their high gravities, which causes all elements apart from the lightest ones to settle out of their atmospheres. The most direct method of probing the inner structure of stars and white dwarfs in detail is via asteroseismology. Here we present a pulsating white dwarf in an eclipsing binary system, enabling us to place extremely precise constraints on the mass and radius of the white dwarf from the lightcurve, independent of the pulsations. This 0.325-solar-mass white dwarf—one member of the SDSS J115219.99+024814.4 system—will serve as a powerful benchmark with which to constrain empirically the core composition of low-mass stellar remnants and to investigate the effects of close binary evolution on the internal structure of white dwarfs

Settling on the group's goals: An n-person argumentation game approach

Argumentation games have been proved to be a robust and flexible tool to resolve conflicts among agents. An agent can propose its explanation and its goal known as a claim, which can be refuted by other agents. The situation is more complicated when there are more than two agents playing the game. We propose a weighting mechanism for competing premises to tackle with conflicts from multiple agents in an n-person game. An agent can defend its proposal by giving a counter-argument to change the ``opinion'' of the majority of opposing agents. During the game, an agent can exploit the knowledge that other agents expose in order to promote and defend its main claim

Correlated effects of ocean acidification and warming on behavioral and metabolic traits of a large pelagic fish

Ocean acidification and warming are co-occurring stressors, yet their effects on early life stages of large pelagic fishes are not well known. Here, we determined the effects of elevated CO2 and temperature at levels projected for the end of the century on activity levels, boldness, and metabolic traits (i.e., oxygen uptake rates) in larval kingfish (Seriola lalandi), a large pelagic fish with a circumglobal distribution. We also examined correlations between these behavioral and physiological traits measured under different treatments. Kingfish were reared from the egg stage to 25 days post-hatch in a full factorial design of ambient and elevated CO2 (~500 µatm and ~1000 µatm) and temperature (21 °C and 25 °C). Activity levels were higher in fish from the elevated temperature treatment compared with fish reared under ambient temperature. However, elevated CO2 did not affect activity, and boldness was not affected by either elevated CO2 or temperature. Both elevated CO2 and temperature resulted in increased resting oxygen uptake rates compared to fish reared under ambient conditions, but neither affected maximum oxygen uptake rates nor aerobic scope. Resting oxygen uptake rates and boldness were negatively correlated under ambient temperature, but positively correlated under elevated temperature. Maximum oxygen uptake rates and boldness were also negatively correlated under ambient temperature. These findings suggest that elevated temperature has a greater impact on behavioral and physiological traits of larval kingfish than elevated CO2. However, elevated CO2 exposure did increase resting oxygen uptake rates and interact with temperature in complex ways. Our results provide novel behavioral and physiological data on the responses of the larval stage of a large pelagic fish to ocean acidification and warming conditions, demonstrate correlations between these traits, and suggest that these correlations could influence the direction and pace of adaptation to global climate change

On the Use of High‐Resolution and Deep‐Learning Seismic Catalogs for Short‐Term Earthquake Forecasts: Potential Benefits and Current Limitations

Enhanced earthquake catalogs provide detailed images of evolving seismic sequences. Currently, these data sets take some time to be released but will soon become available in real time. Here, we explore whether and how enhanced seismic catalogs feeding into established short-term earthquake forecasting protocols may result in higher predictive skill. We consider three enhanced catalogs for the 2016–2017 Central Italy sequence, featuring a bulk completeness lower by at least two magnitude units compared to the real-time catalog and an improved hypocentral resolution. We use them to inform a set of physical Coulomb Rate-and-State (CRS) and statistical Epidemic-Type Aftershock Sequence (ETAS) models to forecast the space-time occurrence of M3+ events during the first 6 months of the sequence. We track model performance using standard likelihood-based metrics and compare their skill against the best-performing CRS and ETAS models among those developed with the real-time catalog. We find that while the incorporation of the triggering contributions from new small magnitude detections of the enhanced catalogs is beneficial for both types of forecasts, these models do not significantly outperform their respective near real-time benchmarks. To explore the reasons behind this result, we perform targeted sensitivity tests that show how (a) the typical spatial discretizations of forecast experiments (urn:x-wiley:21699313:media:jgrb55931:jgrb55931-math-00012 km) hamper the ability of models to capture highly localized secondary triggering patterns and (b) differences in earthquake parameters (i.e., magnitude and hypocenters) reported in different catalogs can affect forecast evaluation. These findings will contribute toward improving forecast model design and evaluation strategies for next-generation seismic catalogs. Key Points We compare retrospective forecast models informed by enhanced versus real-time earthquake catalogs for the 2016–2017 Central Italy sequence To realize the benefits of high-resolution catalogs, models should integrate advanced experimental setups, like finer spatial grids Results stimulate further testing on the optimal design of next-generation forecast models based on enhanced seismic catalog

A fixed-point property of logic-based bargaining solution

Abstract. This paper presents a logic-based bargaining solution based on Zhang and Zhang’s framework. It is shown that if the demand sets of players are logically closed, the solution satisfies a fixed-point property, which says that the outcome of bargaining is the result of mutual belief revision. The result is interesting not only because it presents a desirable logical property of bargaining solution but also establishes a link between bargaining theory and multi-agent belief revision.

Organ health and development in larval kingfish are unaffected by ocean acidification and warming

Anthropogenic CO₂ emissions are causing global ocean warming and ocean acidification. The early life stages of some marine fish are vulnerable to elevated ocean temperatures and CO₂ concentrations, with lowered survival and growth rates most frequently documented. Underlying these effects, damage to different organs has been found as a response to elevated CO₂ in larvae of several species of marine fish, yet the combined effects of acidification and warming on organ health are unknown. Yellowtail kingfish, Seriola lalandi, a circumglobal subtropical pelagic fish of high commercial and recreational value, were reared from fertilization under control (21 °C) and elevated (25 °C) temperature conditions fully crossed with control (500 µatm) and elevated (1,000 µatm) pCO₂ conditions. Larvae were sampled at 11 days and 21 days post hatch for histological analysis of the eye, gills, gut, liver, pancreas, kidney and liver. Previous work found elevated temperature, but not elevated CO₂, significantly reduced larval kingfish survival while increasing growth and developmental rate. The current histological analysis aimed to determine whether there were additional sublethal effects on organ condition and development and whether underlying organ damage could be responsible for the documented effects of temperature on survivorship. While damage to different organs was found in a number of larvae, these effects were not related to temperature and/or CO₂ treatment. We conclude that kingfish larvae are generally vulnerable during organogenesis of the digestive system in their early development, but that this will not be exacerbated by near-future ocean warming and acidification

SDSS J105754.25+275947.5: a period-bounce eclipsing cataclysmic variable with the lowest-mass donor yet measured

We present high-speed, multicolour photometry of the faint, eclipsing cataclysmic variable (CV) SDSS J105754.25+275947.5. The light from this system is dominated by the white dwarf. Nonetheless, averaging many eclipses reveals additional features from the eclipse of the bright spot. This enables the fitting of a parameterised eclipse model to these average light curves, allowing the precise measurement of system parameters. We find a mass ratio of q = 0.0546 $\pm$ 0.0020 and inclination i = 85.74 $\pm$ 0.21$^{\circ}$. The white dwarf and donor masses were found to be M$_{\mathrm{w}}$ = 0.800 $\pm$ 0.015 M$_{\odot}$ and M$_{\mathrm{d}}$ = 0.0436 $\pm$ 0.0020 M$_{\odot}$, respectively. A temperature T$_{\mathrm{w}}$ = 13300 $\pm$ 1100 K and distance d = 367 $\pm$ 26 pc of the white dwarf were estimated through fitting model atmosphere predictions to multicolour fluxes. The mass of the white dwarf in SDSS 105754.25+275947.5 is close to the average for CV white dwarfs, while the donor has the lowest mass yet measured in an eclipsing CV. A low-mass donor and an orbital period (90.44 min) significantly longer than the period minimum strongly suggest that this is a bona fide period-bounce system, although formation from a white dwarf/brown dwarf binary cannot be ruled out. Very few period-minimum/period-bounce systems with precise system parameters are currently known, and as a consequence the evolution of CVs in this regime is not yet fully understood

Equal Intensity Contours for Whole-Body Vibrations Compared with Vibrations Cross-Modally Matched to Isophones

Abstract. In this study, two experiments were conducted to determine the curves of equal intensity perception for sinusoidal vertical whole-body vibrations (WBV) of seated subjects over the frequency range from 10Hz to 250Hz. Vibrations were presented to subjects using a at hard seat. In total, 10 participants were asked to match the intensity of dierent vibrations, using a method of adjustment. The obtained contours were compared with the threshold of vibration and to vibrations cross-modally matched to tones from isophones. The shapes of the equal intensity contours in the present study show reasonable agreement with the contours from other studies despite the use of dierent methodologies and experimental questions. The contours show a characteristic similar to the perception threshold. No dependency of vibration magnitude on the shape of the contours was found in the applied dynamic range. However, large inter-individual variations were observed. The results imply that vibration curves that are cross-modally matched to isophones show similar characteristics