Coring method of sampling potato tubers to detect ralstonia solanacearum.

Bacterial wilt caused by Ralstonia solanacearum is considered among the most damaging diseases of potato in Sub-Saharan Africa. In Kenya, majority of farmers visually select and save seed from harvested potato tubers and reuse the same tubers for several seasons. Latently infected seed tubers which cannot be identified by visual inspection during certification further compounds the situation compelling the need for laboratory testing. The study evaluated the effectiveness of coring tuber samples to improve sampling efficiency for onward laboratory diagnosis. In this study, the coring method of sampling potato tubers for detection R. solanacearum was evaluated. Coring involves taking multiple tuber samples direct from the stolon attachment site into a collection tube containing extraction buffer that provides the extract for further diagnostic tests. Coring was assessed using field samples from different potato growing regions of Kenya including, Koibatek, Molo, Uasin Gishu, Bungoma and Kisii and tested using Nitrocellulose Membrane (NCM) ELISA. These results were compared to PCR, qPCR and LAMP. Coring method was statistically reliable (p>0.05) when compared to the standard sampling method used in Kenya to detect R. solanacearum. The coring of potato tubers is a reliable and quicker method of sampling that reduces the turnaround time of testing hence improving efficiency

Exploring the Expanding Universe and Dark Energy using the Statefinder Diagnostic

The coming few years are likely to witness a dramatic increase in high quality Sn data as current surveys add more high redshift supernovae to their inventory and as newer and deeper supernova experiments become operational. Given the current variety in dark energy models and the expected improvement in observational data, an accurate and versatile diagnostic of dark energy is the need of the hour. This paper examines the Statefinder diagnostic in the light of the proposed SNAP satellite which is expected to observe about 2000 supernovae per year. We show that the Statefinder is versatile enough to differentiate between dark energy models as varied as the cosmological constant on the one hand, and quintessence, the Chaplygin gas and braneworld models, on the other. Using SNAP data, the Statefinder can distinguish a cosmological constant ($w=-1$) from quintessence models with $w \geq -0.9$ and Chaplygin gas models with $\kappa \leq 15$ at the $3\sigma$ level if the value of \om is known exactly. The Statefinder gives reasonable results even when the value of \om is known to only $\sim 20$ accuracy. In this case, marginalizing over \om and assuming a fiducial LCDM model allows us to rule out quintessence with $w \geq -0.85$ and the Chaplygin gas with $\kappa \leq 7$ (both at $3\sigma$). These constraints can be made even tighter if we use the Statefinders in conjunction with the deceleration parameter. The Statefinder is very sensitive to the total pressure exerted by all forms of matter and radiation in the universe. It can therefore differentiate between dark energy models at moderately high redshifts of z \lleq 10.Comment: 21 pages, 17 figures. Minor typos corrected to agree with version published in MNRAS. Results unchange

Finite-temperature scalar fields and the cosmological constant in an Einstein universe

We study the back reaction effect of massless minimally coupled scalar field at finite temperatures in the background of Einstein universe. Substituting for the vacuum expectation value of the components of the energy-momentum tensor on the RHS of the Einstein equation, we deduce a relationship between the radius of the universe and its temperature. This relationship exhibit a maximum temperature, below the Planck scale, at which the system changes its behaviour drastically. The results are compared with the case of a conformally coupled field. An investigation into the values of the cosmological constant exhibit a remarkable difference between the conformally coupled case and the minimally coupled one.Comment: 7 pages, 2 figure

Histone deacetylase 11 inhibition promotes breast cancer metastasis from lymph nodes

Lymph node (LN) metastases correspond with a worse prognosis in nearly all cancers, yet the occurrence of cancer spreading from LNs remains controversial. Additionally, the mechanisms explaining how cancers survive and exit LNs are largely unknown. Here, we show that breast cancer patients frequently have LN metastases that closely resemble distant metastases. In addition, using a microsurgical model, we show how LN metastasis development and dissemination is regulated by the expression of a chromatin modifier, histone deacetylase 11 (HDAC11). Genetic and pharmacologic blockade of HDAC11 decreases LN tumor growth, yet substantially increases migration and distant metastasis formation. Collectively, we reveal a mechanism explaining how HDAC11 plasticity promotes breast cancer growth as well as dissemination from LNs and suggest caution with the use of HDAC inhibitors

The 6dF galaxy survey: fundamental plane data

We report the 6dFGS Fundamental Plane (6dFGSv) catalogue that is used to estimate distances and peculiar velocities for nearly 9000 early-type galaxies in the local (z < 0.055) universe. Velocity dispersions are derived by cross-correlation from 6dF V-band spectra with typical S/N of 12.9 Å−1 for a sample of 11 315 galaxies; the median velocity dispersion is 163 km s−1 and the median measurement error is 12.9 per cent. The photometric Fundamental Plane (FP) parameters (effective radii and surface brightnesses) are determined from the JHK 2MASS images for 11 102 galaxies. Comparison of the independent J- and K-band measurements implies that the average uncertainty in XFP, the combined photometric parameter that enters the FP, is 0.013 dex (3 per cent) for each band. Visual classification of morphologies was used to select a sample of nearly 9000 early-type galaxies that form 6dFGSv. This catalogue has been used to study the effects of stellar populations on galaxy scaling relations, to investigate the variation of the FP with environment and galaxy morphology, to explore trends in stellar populations through, along and across the FP, and to map and analyse the local peculiar velocity field

Charged black holes in quadratic gravity

Iterative solutions to fourth-order gravity describing static and electrically charged black holes are constructed. Obtained solutions are parametrized by two integration constants which are related to the electric charge and the exact location of the event horizon. Special emphasis is put on the extremal black holes. It is explicitly demonstrated that in the extremal limit, the exact location of the (degenerate) event horizon is given by \rp = |e|. Similarly to the classical Reissner-Nordstr\"om solution, the near-horizon geometry of the charged black holes in quadratic gravity, when expanded into the whole manifold, is simply that of Bertotti and Robinson. Similar considerations have been carried out for the boundary conditions of second type which employ the electric charge and the mass of the system as seen by a distant observer. The relations between results obtained within the framework of each method are briefly discussed

Phantom Field with O(N) Symmetry in Exponential Potential

In this paper, we study the phase space of phantom model with O(\emph{N}) symmetry in exponential potential. Different from the model without O(\emph{N}) symmetry, the introduction of the symmetry leads to a lower bound $w>-3$ on the equation of state for the existence of stable phantom dominated attractor phase. The reconstruction relation between the potential of O(\textit{N}) phantom system and red shift has been derived.Comment: 5 pages, 3 figures, replaced with the version to appear on Phys. Rev.

Pellino-1 regulates the responses of the airway to viral infection

Exposure to respiratory pathogens is a leading cause of exacerbations of airway diseases such as asthma and chronic obstructive pulmonary disease (COPD). Pellino-1 is an E3 ubiquitin ligase known to regulate virally-induced inflammation. We wished to determine the role of Pellino-1 in the host response to respiratory viruses in health and disease. Pellino-1 expression was examined in bronchial sections from patients with GOLD stage two COPD and healthy controls. Primary bronchial epithelial cells (PBECs) in which Pellino-1 expression had been knocked down were extracellularly challenged with the TLR3 agonist poly(I:C). C57BL/6 Peli1−/− mice and wild type littermates were subjected to intranasal infection with clinically-relevant respiratory viruses: rhinovirus (RV1B) and influenza A. We found that Pellino-1 is expressed in the airways of normal subjects and those with COPD, and that Pellino-1 regulates TLR3 signaling and responses to airways viruses. In particular we observed that knockout of Pellino-1 in the murine lung resulted in increased production of proinflammatory cytokines IL-6 and TNFα upon viral infection, accompanied by enhanced recruitment of immune cells to the airways, without any change in viral replication. Pellino-1 therefore regulates inflammatory airway responses without altering replication of respiratory viruses

Survival, pathologic response, and genomics in CALGB 40601 (Alliance), a neoadjuvant Phase III trial of paclitaxel-trastuzumab with or without lapatinib in HER2-positive breast cancer

PURPOSE CALGB 40601 assessed whether dual versus single human epidermal growth factor receptor 2 (HER2) -targeting drugs added to neoadjuvant chemotherapy increased pathologic complete response (pCR). Here, we report relapse-free survival (RFS), overall survival (OS), and gene expression signatures that predict pCR and survival. PATIENTS AND METHODS Three hundred five women with untreated stage II and III HER2-positive breast cancer were randomly assigned to receive weekly paclitaxel combined with trastuzumab plus lapatinib (THL), trastuzumab (TH), or lapatinib (TL). The primary end point was pCR, and secondary end points included RFS, OS, and gene expression analyses. mRNA sequencing was performed on 264 pretreatment samples. RESULTS One hundred eighteen patients were randomly allocated to THL, 120 to TH, and 67 to TL. At more than 7 years of follow-up, THL had significantly better RFS and OS than did TH (RFS hazard ratio, 0.32; 95% CI, 0.14 to 0.71; P 5.005; OS hazard ratio, 0.34; 95% CI, 0.12 to 0.94; P 5.037), with no difference between TH and TL. Of 688 previously described gene expression signatures, significant associations were found in 215 with pCR, 45 with RFS, and only 22 with both pCR and RFS (3.2%). Specifically, eight immune signatures were significantly correlated with a higher pCR rate and better RFS. Among patients with residual disease, the immunoglobulin G signature was an independent, good prognostic factor, whereas the HER2-enriched signature, which was associated with a higher pCR rate, showed a significantly shorter RFS. CONCLUSION In CALGB 40601, dual HER2-targeting resulted in significant RFS and OS benefits. Integration of intrinsic subtype and immune signatures allowed for the prediction of pCR and RFS, both overall and within the residual disease group. These approaches may provide means for rational escalation and de-escalation treatment strategies in HER2-positive breast cancer