Use of microwave remote sensors for determination of water in sub-soils

Student supported: 1 Graduate AssistantThe development of instruments for rapidly measuring the water content of subsoils is important in many areas of water resources research. For example, knowing the excess or lack of subsoil water can improve crops. The marked dielectric contrast, which exists between water and other common soil constituents (Fig. 1 and Table I) at microwave frequencies, provides a valuable means of sensing water content in soil.Project # A-070-MO Agreement # 14-31-0001-402

Findings from the California Youth Transitions to Adulthood Study (CalYOUTH): Conditions of Youth at Age 19

The "CalYOUTH Wave 2 Youth Survey", conducted when the young people participating in CalYOUTH were 19 years old, follows up on a survey of the same young people when they were approaching the age of majority in California's foster care system. More than 80 percent of the youth who took part in the baseline interviews participated in the Wave 2 survey. The report provides the most comprehensive view to date of young adults making the transition to adulthood from foster care in California, highlighting differences between young people participating in extended foster care and young people who had left care. The report provides feedback for all parties interested in improving youth's transitions from foster care to adulthood

OSU-03012 sensitizes breast cancers to lapatinib-induced cell killing: a role for Nck1 but not Nck2

Background Lapatinib is characterized as an ErbB1/ErbB2 dual inhibitor and has recently been approved for the treatment of metastatic breast cancer. In this study, we examined mechanisms associated with enhancing the activity of lapatinib via combination with other therapies. Methods In the present studies, estrogen receptor (ER) positive and ER negative breast cancer cells were genetically manipulated to up- or downregulate eIF2-alpha, its phospho-mutant, Nck1, or Nck2, then treated with OSU-03012, lapatinib or the combination and assayed for cytotoxicity/cytostaticity using clonogenic assays. Results Treatment of breast cancer cell lines with lapatinib and OSU-03012 (a small molecule derivative of the Cox-2 inhibitor celecoxib) induced synergistic cytotoxic/cytostatic effects. This combination therapy corresponded to an increase in the phosphorylation of eIF2-α at serine51 and a decrease in Nck1 expression. Ectopic expression of phospho-mutant eIF2-α (Ser51Ala) or downregulation of eIF2-α in addition to downregulation of the eIF2-α kinase PERK inhibited the synergistic and cytotoxic effects. Furthermore, ectopic expression of Nck1, but not Nck2 abolished the decrease in cell viability observed in combination-treated cells. Downregulation of Nck1 failed to “rescue” the ablation of the cytotoxic/cytostatic effects by the phospho-mutant of eIF2-α (Ser51Ala) demonstrating that Nck1 downregulation is upstream of eIF2-α phosphorylation in the anti-survival pathway activated by lapatinib and OSU-03012 treatment. Finally, co-immunoprecipitation assays indicated that eIF2-α dissociates from the Nck1/PP1 complex after OSU-03012 and lapatinib co-treatment. Conclusions These data indicate that OSU-03012 and lapatinib co-treatment is an effective combination therapy, which functions to enhance cell killing through the Nck1/eIF2 complex. Hence, this complex is a novel target for the treatment of metastatic breast cancer

Reaction-diffusion dynamics: confrontation between theory and experiment in a microfluidic reactor

We confront, quantitatively, the theoretical description of the reaction-diffusion of a second order reaction to experiment. The reaction at work is \ca/CaGreen, and the reactor is a T-shaped microchannel, 10 $\mu$m deep, 200 $\mu$m wide, and 2 cm long. The experimental measurements are compared with the two-dimensional numerical simulation of the reaction-diffusion equations. We find good agreement between theory and experiment. From this study, one may propose a method of measurement of various quantities, such as the kinetic rate of the reaction, in conditions yet inaccessible to conventional methods

Effect of the National Resident Assessment Instrument on Selected Health Conditions and Problems

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111253/1/j.1532-5415.1997.tb02972.x.pd

The Wide Integral Field Infrared Spectrograph: Commissioning Results and On-sky Performance

We have recently commissioned a novel infrared ($0.9-1.7$ $\mu$m) integral field spectrograph (IFS) called the Wide Integral Field Infrared Spectrograph (WIFIS). WIFIS is a unique instrument that offers a very large field-of-view (50$^{\prime\prime}$ x 20$^{\prime\prime}$) on the 2.3-meter Bok telescope at Kitt Peak, USA for seeing-limited observations at moderate spectral resolving power. The measured spatial sampling scale is $\sim1\times1^{\prime\prime}$ and its spectral resolving power is $R\sim2,500$ and $3,000$ in the $zJ$ ($0.9-1.35$ $\mu$m) and $H_{short}$ ($1.5-1.7$ $\mu$m) modes, respectively. WIFIS's corresponding etendue is larger than existing near-infrared (NIR) IFSes, which are mostly designed to work with adaptive optics systems and therefore have very narrow fields. For this reason, this instrument is specifically suited for studying very extended objects in the near-infrared such as supernovae remnants, galactic star forming regions, and nearby galaxies, which are not easily accessible by other NIR IFSes. This enables scientific programs that were not originally possible, such as detailed surveys of a large number of nearby galaxies or a full accounting of nucleosynthetic yields of Milky Way supernova remnants. WIFIS is also designed to be easily adaptable to be used with larger telescopes. In this paper, we report on the overall performance characteristics of the instrument, which were measured during our commissioning runs in the second half of 2017. We present measurements of spectral resolving power, image quality, instrumental background, and overall efficiency and sensitivity of WIFIS and compare them with our design expectations. Finally, we present a few example observations that demonstrate WIFIS's full capability to carry out infrared imaging spectroscopy of extended objects, which is enabled by our custom data reduction pipeline.Comment: Published in the Proceedings of SPIE Astronomical Telescopes and Instrumentation 2018. 17 pages, 13 figure

Five Years of Mid-Infrared Evolution of the Remnant of SN 1987A: The Encounter Between the Blast Wave and the Dusty Equatorial Ring

We have used the Spitzer satellite to monitor the mid-IR evolution of SN 1987A over a 5 year period spanning the epochs between days 6000 and 8000 since the explosion. The supernova (SN) has evolved into a supernova remnant (SNR) and its radiative output is dominated by the interaction of the SN blast wave with the pre-existing equatorial ring (ER). The mid-IR spectrum is dominated by emission from ~180 K silicate dust, collisionally-heated by the hot X-ray emitting gas with a temperature and density of ~5x10^6 K and 3x10^4 cm-3, respectively. The mass of the radiating dust is ~1.2x10^(-6) Msun on day 7554, and scales linearly with IR flux. The infrared to soft-X-ray flux ratio is roughly constant with a value of 2.5. Gas-grain collisions therefore dominate the cooling of the shocked gas. The constancy of of this ratio suggests that very little grain processing or gas cooling have occurred throughout this epoch. The shape of the dust spectrum remained unchanged during the observations while the total flux increased with a time dependence of t^(0.87), t being the time since the first encounter between the blast wave and the ER. These observations are consistent with the transitioning of the blast wave from free expansion to a Sedov phase as it propagates into the main body of the ER.Comment: Accepted for publication in the ApJ, 11 pages, 11 figure

Functional equivalence of stem cell and stem cell-derived extracellular vesicle transplantation to repair the irradiated brain.

Cranial radiotherapy, although beneficial for the treatment of brain tumors, inevitably leads to normal tissue damage that can induce unintended neurocognitive complications that are progressive and debilitating. Ionizing radiation exposure has also been shown to compromise the structural integrity of mature neurons throughout the brain, an effect believed to be at least in part responsible for the deterioration of cognitive health. Past work has shown that cranially transplanted human neural stem cells (hNSCs) or their extracellular vesicles (EVs) afforded long-term beneficial effects on many of these cognitive decrements. To provide additional insight into the potential neuroprotective mechanisms of cell-based regenerative strategies, we have analyzed hippocampal neurons for changes in structural integrity and synaptic remodeling after unilateral and bilateral transplantation of hNSCs or EVs derived from those same cells. Interestingly, hNSCs and EVs similarly afforded protection to host neurons, ameliorating the impact of irradiation on dendritic complexity and spine density for neurons present in both the ipsilateral and contralateral hippocampi 1 month following irradiation and transplantation. These morphometric improvements were accompanied by increased levels of glial cell-derived growth factor and significant attenuation of radiation-induced increases in postsynaptic density protein 95 and activated microglia were found ipsi- and contra-lateral to the transplantation sites of the irradiated hippocampus treated with hNSCs or hNSC-derived EVs. These findings document potent far-reaching neuroprotective effects mediated by grafted stem cells or EVs adjacent and distal to the site of transplantation and support their potential as therapeutic agents to counteract the adverse effects of cranial irradiation

KAAS: an automatic genome annotation and pathway reconstruction server

The number of complete and draft genomes is rapidly growing in recent years, and it has become increasingly important to automate the identification of functional properties and biological roles of genes in these genomes. In the KEGG database, genes in complete genomes are annotated with the KEGG orthology (KO) identifiers, or the K numbers, based on the best hit information using Smith–Waterman scores as well as by the manual curation. Each K number represents an ortholog group of genes, and it is directly linked to an object in the KEGG pathway map or the BRITE functional hierarchy. Here, we have developed a web-based server called KAAS (KEGG Automatic Annotation Server: http://www.genome.jp/kegg/kaas/) i.e. an implementation of a rapid method to automatically assign K numbers to genes in the genome, enabling reconstruction of KEGG pathways and BRITE hierarchies. The method is based on sequence similarities, bi-directional best hit information and some heuristics, and has achieved a high degree of accuracy when compared with the manually curated KEGG GENES database