Propriétés rhéologiques de farines panifiables formulées à partir d’extraits d’amidon et de gluten de blé

Le présent travail a mis en évidence les propriétés rhéologiques des farines panifiables reconstituées avec des extraits d’amidon. Du gluten vital 10% a été incorporé à 90% d’extraits d’amidons de blé, de maïs,de riz et de la pomme de terre pour obtenir, respectivement, quatre (4) types de farines F1, F2, F3 et F4. Les farines ont été reconstituées à l’aide d’un mélangeur de type KENWOOD à 4 unités de vitesse. Les caractéristiques rhéologiques des pâtes des farines reconstituées ont été analysées à l’aide du Farinographe Brabender et de l’Alvéographe Chopin. Les farines F2, F3 et F4 sont caractérisées par une forte capacité d’hydratation, une faible activité a-amylasique qui se traduit par des temps de chute très élevés. Il en résulte des pâtes de très faible tenue avec une résistance à l’extraction, en comparaison avec une farine de référence (Fo). La force de la pâte de la farine F1 est plus élevée (W = 85.10-4 J) que celle des pâtes des farines F2, F3 et F4. Globalement, l’équilibre de configuration (P/L) entre la ténacité et l’élasticité des pâtes des farines est de l’ordre de 0,88 alors que les indices d’élasticité varient entre 7,3 et 9,4

Kinetics of the hydrolysis of polysaccharide galacturonic acid and neutral sugars chains from flaxseed mucilage

Different hydrolysis procedures of flaxseed polysaccharides (chemical and enzymatic) were carried out with H2SO4, HCl and TFA at different acid concentrations (0.2, 1 and 2 M) and temperatures (80 and 100°C). Enzymatic and combined chemical and enzymatic hydrolyses of polysaccharide from flaxseed mucilage were also studied. Acid hydrolysis conditions (2 M H2SO4, 4 h, 100°C) are required to quantify total monosaccharide content of flaxseed mucilage. The enzymatic pathway (Pectinex™ Ultra SP) limits sugar destruction during hydrolysis, but it is also insufficient for complete depolymerization. The combination of the two treatments, i.e. moderate chemical hydrolysis (0.2 M H2SO4, 80°C, 48 h) combined with enzymatic hydrolysis is not more effective compared to chemical hydrolysis in drastic conditions (2 M H2SO4 at 100°C). The strong interaction between the neutral and acid fractions of flaxseed mucilage may hinder total release of sugar residues. Physical treatment prior to the hydrolysis could be necessary to achieve complete depolymerisation of flaxseed mucilage

The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca

Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the mode of action involved in their biocontrol performance. Cell-free supernatants showed antifungal activities very close to those previously reported for vegetative cells. Identification of three lipopeptide antibiotics, surfactin, fengycin, and iturin A or bacillomycin, in butanolic extracts from cell-free culture filtrates of these B. subtilis strains pointed out that antibiosis could be a major factor involved in their biocontrol ability. The strong inhibitory effect of purified lipopeptide fractions corresponding to bacillomycin, fengycin, and iturin A on P. fusca conidia germination, as well as the in situ detection of these lipopeptides in bacterial-treated melon leaves, provided interesting evidence of their putative involvement in the antagonistic activity. Those results were definitively supported by site-directed mutagenesis analysis, targeted to suppress the biosynthesis of the different lipopeptides. Taken together, our data have allowed us to conclude that the iturin and fengycin families of lipopeptides have a major role in the antagonism of B. subtilis toward P. fusca.

The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca

Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the mode of action involved in their biocontrol performance. Cell-free supernatants showed antifungal activities very close to those previously reported for vegetative cells. Identification of three lipopeptide antibiotics, surfactin, fengycin, and iturin A or bacillomycin, in butanolic extracts from cell-free culture filtrates of these B. subtilis strains pointed out that antibiosis could be a major factor involved in their biocontrol ability. The strong inhibitory effect of purified lipopeptide fractions corresponding to bacillomycin, fengycin, and iturin A on P. fusca conidia germination, as well as the in situ detection of these lipopeptides in bacterial-treated melon leaves, provided interesting evidence of their putative involvement in the antagonistic activity. Those results were definitively supported by site-directed mutagenesis analysis, targeted to suppress the biosynthesis of the different lipopeptides. Taken together, our data have allowed us to conclude that the iturin and fengycin families of lipopeptides have a major role in the antagonism of B. subtilis toward P. fusca.

Characterization of apple seed oil with Denomination of Origin from Asturias, Spain

The content and composition of lipids isolated from the seeds of seven apple species from Asturias (Spain) were characterized. The highest content in oil corresponded to Collao (22.73±0.81 g·100 g^-1 of seed), followed by Raxao and Riega (20.19±0.74 g·100 g^-1 of seed; 19.67±0.85 g·100 g^-1 of seed), respectively. The linoleic acid was found to be the main component in Limón Montés (60.78±3.07%) followed by Riega (60.01±3.41%). Solarina seed oil was the one with the highest content in total sterols (558.52±9.42 mg·100 g^-1 of oil) while Blanquina was the one that presents the lowest amount (166.55±1.89 mg·100 g^-1 of oil). Phosphatidylcholine (70.58±3.85%) was found to be the main constituent in Blanquina followed by Collaos (55.55±2.96%). Raxao presented the highest content in β-tocopherol (125.29±12.62) and α-tocopherol was the most important tocopherol in Limón Montés (84.68±5.61 mg·kg^-1 of oil). The main triglycerides were LLP (41.17±1.98-39.32±1.66%) followed by LLL (27.12±1.32−17.80±1.96%). Good separation among specie samples according to the statistical analysis of the principal component (PCs) and linear discriminant analysis (LDA) was obtained.<br><br>Se ha caracterizado el contenido y composición de los lípidos aislados de semillas de siete especies de manzanas de Asturias (España). El contenido más alto en aceite correspondió a Collao (22.73±0.81 g·100 g^-1 de semilla), seguida de Raxao y Riega (20.19±0.74 g·100 g^-1 de semilla y 19.67±0.85 g·100 g^-1 de semilla) respectivamente. El ácido linoleico fué el de mayor contenido en Limón Montés (60.78±3.07%) seguido por la Riega (60.01±3.41%). El contenido más alto en esteroles correspondió a Solarina (558.52±9.42 mg·100 g^-1 de aceite) mientras que Blanquina fué el que presentó los valores más bajo (166.55±1.89 mg·100 g^-1 de aceite). Fosfatidilcolina (70.58±3.85% del total FL) fué el principal constituyente en Blanquina seguida por Collao (55.55±2.96% del total FL). Raxao presentó el contenido más alto en β-tocoferol (125.29±12.62 mg·Kg^-1 de aceite) y α-tocoferol en Limón Montés (84.68±5.61 mg·Kg^-1 de aceite). Los triglicéridos principales fueron LLP (41.17±1.98-39.32±1.66%) seguido de LLL (27.12±1.32−17.80±1.96%). Se obtuvo una buena separación entre las distintas variedades de manzana teniendo en cuenta el análisis estadístico de los principales componentes (PCs) y el análisis discriminante linear (LDA)

Determination of iodine value of palm oil based on triglyceride composition

The triglyceride (TG) composition of palm oil is normally determined by high-performance liquid chromatography (HPLC). The HPLC chromatograms indicated a good separation of most of the TG components in the oil. The TG can be classified based on either the TG groups, i.e., triunsaturated, monosaturated, disaturated, or trisaturated, or the number of double bonds, i.e., zero, one, two, three, or four double bonds. The more unsaturated the fatty acid, the greater the iodine value (IV). Therefore, it is hypothesized that the IV of an oil can be determined based upon the TG composition of the oil. Based on the TG groups, stepwise regression analysis showed that the areas of the disaturated, trisaturated, and triunsaturated TG peaks could predict the IV with a coefficient of determination (R2) of 0.990. The regression based on the number of double bonds yielded a good regression equation with R2=0.992. The important variables were the peak area of the fatty acids that contained zero, one, two, and three double bonds. This study concludes that the TG composition can be used to predict the IV of palm oil. The best prediction model is obtained by using the number of double bonds in the TG as the independent variable

Butylated caffeic acid: An efficient novel antioxidant

A novel antioxidant, butylated caffeic acid (BCA) was rationally designed by adding a tert-butyl group to caffeic acid, which was synthesized at a high yield (36.2%) from 2-methoxy-4-methylphenol by a four-step reaction including Friedel-Crafts alkylation, bromine oxidation, ether bond hydrolysis and Knoevenagel condensation. Its antioxidant capacity was much stronger than common commercial antioxidant tert-butyl hydroquinone (TBHQ) and its mother compound, caffeic acid, in both rancimat and deep frying tests. When investigated via the DPPH method, the antioxidant capacity of BCA was almost equal to TBHQ, but lower than caffeic acid. BCA could be a potentially strong antioxidant, especially for food processing at high temperatures such as deep frying and baking

Insulin resistance causes inflammation in adipose tissue

Obesity is a major risk factor for insulin resistance and type 2 diabetes. In adipose tissue, obesity-mediated insulin resistance correlates with the accumulation of proinflammatory macrophages and inflammation. However, the causal relationship of these events is unclear. Here, we report that obesity-induced insulin resistance in mice precedes macrophage accumulation and inflammation in adipose tissue. Using a mouse model that combines genetically induced, adipose-specific insulin resistance (mTORC2-knockout) and diet-induced obesity, we found that insulin resistance causes local accumulation of proinflammatory macrophages. Mechanistically, insulin resistance in adipocytes results in production of the chemokine monocyte chemoattractant protein 1 (MCP1), which recruits monocytes and activates proinflammatory macrophages. Finally, insulin resistance (high homeostatic model assessment of insulin resistance [HOMA-IR]) correlated with reduced insulin/mTORC2 signaling and elevated MCP1 production in visceral adipose tissue from obese human subjects. Our findings suggest that insulin resistance in adipose tissue leads to inflammation rather than vice versa