Zooarqueología santiagueña. Despertando de la siesta

Fil: del Papa, Luis Manuel. Cátedra de Anatomía comparada. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: De Santis, Luciano José María. Cátedra de Anatomía comparada. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Togo, José. Facultad de Humanidades. Ciencias Sociales y de la Salud. Universidad Nacional de Santiago del Estero; Argentin

Sperm characters of the digenean Nephrotrema truncatum (Troglotrematidae): a kidney parasite of Crocidura russula (Soricidae) and their phylogenetic significance

Spermatological characteristics of the troglotrematid digenean Nephrotrema truncatum, a parasite of the shrew Crocidura russula, have been investigated by means of transmission electron microscopy. The ultrastructural study reveals that the mature spermatozoon of N. truncatum exhibits many ultrastructural characters previously described in most gorgoderoideans. These are two axonemes of the 9+'1' trepaxonematan pattern, four attachment zones, a lateral expansion, an external ornamentation of the plasma membrane associated with spine-like bodies and cortical microtubules, and in the posterior part of the anterior spermatozoon region, two bundles of parallel cortical microtubules with the maximum number located in the anterior part of the spermatozoon, a nucleus, two mitochondria, and granules of glycogen. The obtained results are compared with those of other digeneans, particularly the Gorgoderoidea. The sperm cells gorgoderoideans are of type IV, characterised by a 9+'1' pattern of axonemes, the presence of an external ornamentation associated with cortical microtubules and located in the posterior area of the anterior extremity, the presence of two bundles of cortical microtubules, the maximum number of cortical microtubules located in the anterior region of the spermatozoon, and the presence of generally two mitochondria. However, dicrocoeliids and troglotrematids have spermatozoa with ornamentation of the plasma membrane and lateral expansions

Phenotypic, Molecular and Symbiotic Characterization of the Rhizobial Symbionts of Desmanthus paspalaceus (Lindm.) Burkart That Grow in the Province of Santa Fe, Argentina

Desmanthus paspalaceus (Lindm.) Burkart belongs to the D. virgatus complex, subfamily Mimosoidae. The known potential as livestock fodder of several of these legumes prompted us to undertake a phenotypic, molecular, and symbiotic characterization of the D. paspalaceus symbionts in the Santa Fe province, Argentina. The rhizobia collected—containing isolates with different abiotic-stress tolerances—showed a remarkable genetic diversity by PCR fingerprinting, with 11 different amplification profiles present among 20 isolates. In selected isolates 16S-rDNA sequencing detected mesorhizobia (60%) and rhizobia (40%) within the collection, in contrast to the genus of the original inoculant strain CB3126—previously isolated from Leucaena leucocephala—that we typified here through its 16S rDNA as Sinorhizobium terangae. The results revealed the establishment by diverse bacterial genera -rhizobia, sinorhizobia, and mesorhizobia- of full N2-fixing symbiotic associations with D. paspalaceus. This diversity was paralleled by the presence of at least two different nodC allelic variants. The identical nodC alleles of the Mesorhizobia sp. 10.L.4.2 and 10.L.5.3 notably failed to group within any of the currently described rhizo-/brady-/azorhizobial nodC clades. Interestingly, the nodC from S. terangae CB3126 clustered close to homologs from common bean nodulating rhizobia, but not with the nodC from S. terangae WSM1721 that nodulates Acacia. No previous data were available on nod-gene phylogeny for Desmanthus symbionts. A field assay indicated that inoculation of D. paspalaceus with the local Rhizobium sp. 10L.11.4 produced higher aerial-plant dry weights compared to S. teranga CB3126–inoculated plants. Neither the mesorhizobia 10.L.4.2 or 10.L.5.3 nor the rhizobium 10L.11.4 induced root nodules in L. leucocephala or P. vulgaris. The results show that some of the local isolates have remarkable tolerances to several abiotic stresses including acidity, salt, and temperature; while exhibiting prominent N2 fixation; thus indicating suitability as candidates for inoculation of D. paspalaceus.Fil: Fornasero, Laura Viviana. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: del Papa, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: López, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Albicoro, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Zabala, Juan Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Toniutti, Maria Antonieta. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Pensiero, Jose Francisco. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentin

Resíduos de carbossulfan e de seus metabólitos carbofuran e 3-hidroxi-carbofuran em laranja

The objectives of this study were to evaluate the residues of the insecticide carbosulfan and its carbofuran metabolites and 3-hydroxy-carbofuran in orange compartments (whole fruit, bagasse and juice) and comparison between the residual levels found in fruits with the maximum residue level and the safety interval established by the Brazilian legislation. Two field experiments were carried out, both with the following treatments: a-check; b-one application of 10 g of carbosulfan . 100 L-1 of water; c-one application with twice the rate applied in treatment b; d-four applications with the same rate applied in treatment b. Samples were taken at (-1), zero, 1, 3, 7, 14, 21 and 28 days after the last or unique application. The quantitative determinations were done by gas chromatography technique, using a nitrogen-phosphorus detector. The carbosulfan metabolism to its carbofuran metabolite was rapid (3 days), being both analytes concentrated in the bagasse (peel + flavedo + albedo). However, the metabolism of carbofuran to 3-hydroxy-carbofuran was of low intensity or this metabolite was quickly dissipated. Carbosulfan residues and its metabolites did not penetrate into the fruit, thus not contaminating the juice. The use of the pesticide was adequate, with respect to fruit consumption, in relation to the Brazilian legislation.Os objetivos do estudo foram avaliar os resíduos do inseticida carbossulfan e de seus metabólitos carbofuran e 3-hidroxi-carbofuran em compartimentos (frutos inteiros, bagaço e suco) e a comparação entre os níveis residuais encontrados nos frutos com o limite máximo de resíduo e o intervalo de segurança estabelecidos pela legislação brasileira. Foram conduzidos dois experimentos de campo, ambos com os seguintes tratamentos: a - testemunha; b - uma aplicação de 10 g de carbossulfan. 100 L-1 de água; c - uma aplicação com o dobro da dosagem do tratamento b; d - quatro aplicações na dosagem do tratamento b. As amostras foram colhidas a: (-1); zero; 1; 3; 7; 14; 21 e 28 dias após a última ou única aplicação. As determinações quantitativas foram feitas por técnica de cromatografia em fase gasosa, usando detector de nitrogênio-fósforo. O metabolismo de carbossulfan a seu metabólito carbofuran foi rápido (3 dias), sendo ambos os analitos concentrados no bagaço (casca+flavedo+albedo). Entretanto, o metabolismo de carbofuran a 3-hidroxi-carbofuran foi de baixa intensidade ou este metabólito foi rapidamente dissipado. Resíduos de carbossulfan e de seus metabólitos não penetraram no interior do fruto, não contaminando, assim, o suco. O uso do agrotóxico mostrou-se adequado, com respeito ao consumo da fruta, em relação à legislação brasileira.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade de São Paulo Escola Superior de Agricultura Luis de Queirós Departamento de Produção VegetalUniversidade de São Paulo Escola Superior de Agricultura Luis de Queirós Departamento de Entomologia, Fitopatologia e Zoologia AgrícolaUNIFESP FEIS Departamento de Fitossanidade, Engenharia Rural e SolosUNIFESP, FEIS Depto. de Fitossanidade, Engenharia Rural e SolosSciEL

Long-term efficacy and safety profiles of iris-fixated foldable anterior chamber phakic intraocular lens implantation in eyes with more than 10-years of follow-up

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Cell stress by phosphate of two protozoa tetrahymena thermophila and tetrahymena pyriformis

Phosphorus is one of the bioelements most needed as a compound cell by living organisms. Phosphorus is involved in several pathologies: in human with bone and kidney diseases, in mammals with metabolism disorder (glucose, insulin···), in microorganisms whose phosphorus is involved in cell growth. Phosphorus has various forms including pyrophosphate, a by-product of multiple pathways of biosynthesis. Enzymes that hydrolyze pyrophosphate are called inorganic pyrophosphatases (PPases). Two major types of inorganic pyrophosphatases are distinguished: the soluble pyrophosphatases (sPPases) and the membrane pyrophosphatases (mPPases or H+/Na+-PPases). They play a key role in the control of intracellular inorganic pyrophosphate level and produce an important ions gradient (H+ or Na+) to the cells. In this work, we primarily focused on the physiological study in a phosphate-poor medium of two models Tetrahymena thermophile and Tetrahymena pyriformis, following the mobility, the growth and the morphology of cells. Secondly, we evaluated the enzymatic activity of soluble and membrane pyrophosphatases in both species grown in the same complex medium. A decrease of cell growth is correlated with unusual morphologies and different mobility in the stress medium. The measurement of soluble and membrane inorganic pyrophosphatases activities also shows a decrease which illustrates the lack of phosphate found in the stress medium. Deficiency of phosphate is a limiting factor for protozoan growth. These results indicate that Tetrahymena can be used as a model of cellular stress and consists of a target to study inorganic pyrophosphatases for a better understanding of phosphate cycle in higher organisms

Inorganic Pyrophosphatases: Study of Interest

Inorganic pyrophosphatases are enzymes that catalyze the hydrolysis of inorganic pyrophosphate to orthophosphate. These enzymes are divided into two groups: the soluble pyrophosphatases and the membrane pyrophosphatases. They vary in structure and each has a determined catalysis mechanism. Soluble pyrophosphatases are ubiquitous enzymes and play a key role in regulating the rate of pyrophosphate and balance in this sense, the biosynthetic reactions. Membrane pyrophosphatases are ion pumps, producing a proton or sodium gradient, and provide critical energy reserves to organisms, especially during stress conditions. Several studies have shown that these enzymes are involved in numerous disorders (diseases, fault cell growth∙∙∙). However they are potential targets for the development of agents against parasites. This article consists of a description of the different types, structures, catalytic properties of inorganic pyrophosphatases and their involvement in cellular metabolism

Photorefractive keratectomy after DMEK for corneal decompensation by phakic IOL

Purpose: To provide the first description of photorefractive keratectomy (PRK) for the correction of mild residual refractive error after Descemet membrane endothelial keratoplasty (DMEK). Methods: A case report. Results: A 45-year-old woman presenting with a phakic intraocular lens (PIOL)-related corneal decompensation underwent staged DMEK surgery following PIOL explantation and cataract surgery. Eighteen months after DMEK, uncorrected distance visual acuity (UDVA) was 20/60 and best-corrected visual acuity (BCVA) was 20/22, with stable refraction. The patient requested refractive surgery to decrease spectacle dependence, and wavefront-optimized PRK was performed. At the last follow-up observation thirty-three months after PRK (54 months after DMEK surgery), UDVA was 20/20, the cornea remained clear without signs of rejection or endothelial failure, and the endothelial cell loss rate was not accelerated after PRK. Conclusion: Since long-term visual and refractive stability can be expected after DMEK, PRK may be a particularly safe and effective approach for the correction of mild residual refractive errors after DMEK. However, we consider that surgeons must exercise caution when considering keratorefractive surgery in these eyes due to postoperative changes in corneal curvature and thickness, and further studies are encouraged.info:eu-repo/semantics/publishedVersio

Catecismo romano compuesto por decreto del sagrado Concilio Tridentino, para los parrocos de toda la iglesia, y publicado por San Pio Quinto

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Estrategias de subsistencia de la etapa agroalfarera en la cuenca media del Río Dulce (provincia de Santiago del Estero, Argentina)

En este trabajo se pretende caracterizar las estrategias de subsistencia llevadas a cabo en un sector de la región arqueológica Chaco-Santiagueña en la actual provincia de Santiago del Estero. Se analizaron cuatro sitios arqueológicos del sector de la cuenca media del río Dulce que corresponden a la etapa agroalfarera de la región (ca. 350 DC hasta la conquista española en el siglo XVI), pero de distinto contexto cultural, uno Las Mercedes, dos Sunchitúyoj y uno Averías. Con el fin de caracterizar las estrategias de subsistencia a nivel regional y cultural, se comparó a los sitios a través de los índices de Riqueza, Diversidad y Equitatividad en base a las especies que fueron utilizadas (evidencias directas) o que posiblemente fueron utilizadas por los humanos (evidencias indirectas o sin evidencias que nos permita descartarlo del consumo humano). De los resultados se desprende que en la cuenca media del río Dulce se desarrolló un uso más diverso y una mayor proporción de fauna de menor retorno energético para los inicios de las sociedades agroalfareras de contexto Las Mercedes; y una preponderancia de los recursos de mayor retorno energético para los momentos tardíos, de contexto Sunchitúyoj y Averías.The aim of this paper is to characterize subsistence strategies undertaken in an area of Chaco-Santiagueña archaeological region, current Santiago del Estero province). Four archaeological sites of the Dulce river middle basin area from agro-pottery stage were analyzed (ca. 350 AD until the Spanish conquest in the sixteenth century). The archaeological sites correspond to different cultural contexts (one Las Mercedes, two Sunchitúyoj and one Averías). In order to characterize the subsistence strategies at both regional and cultural level, the archaeological sites were compared through Richness, Diversity and Evenness indexes considering the taxa that were used (direct evidence) or that may have been used by humans (indirect evidence or no evidence that allows us to dismiss human consumption). The results show that at the Dulce river middle basin beginnings of agro-pottery societies; Las Mercedes context a more diverse use and a higher proportion of low energy return fauna was developed. In contrast, for later times -Sunchitúyoj and Averías context-, a preponderance of higher energy return resources is observed.Este trabalho tem como objetivo caracterizar as estratégias de subsistência realizadas em um setor da região arqueológica Chaco-Santiagueña, atual província de Santiago del Estero. Analisaram se quatro sítios arqueológicos no meio da bacia do rio Dulce, correspondente a fase agroalfarera (ca. 350 DC até a conquista espanhola do século XVI). Porém com contexto cultural diferentes, foram analisados um no das Mercedes, dois no de Sunchitúyoj e um em Averías. Com o propósito de caracterizar as estratégias de subsistência a nível regional e cultural, foram comparados os sítios com bases nos índices de riqueza, diversidade e uniformidade das espécies que foram utilizadas (evidencias direta) ou que podem ter sido usados por seres humanos (evidencia indireta ou sem provas, que nos permitam descartar que foram utilizadas por humanos). Os resultados mostram que no meio da bacia do rio Dulce, ouve uma utilização diversificada em maior proporção da fauna, e com menor retorno energético para o inicio das sociedades agroalfareras, no contexto das Mercedes; foi desenvolvida uma predominância dos recursos de maior retorno energético para os processos tardios dos sítios de Sunchitúyoj e Averías.Facultad de Ciencias Naturales y Muse