Studio CFD sull'efflusso da valvole a fungo per iniezione di idrogeno in MCI

Nel lavoro di tesi è stato preso in esame un'innovativo sistema di iniezione diretta di idrogeno a bassa pressione ideato e sviluppato presso il Dipartimento di Energetica della Facoltà di Ingegneria di Pisa. La tesi è a carattere numerico e riguarda simulazioni CFD realizzate con il codice AVL Fire. Parte della'attività di simulazione ha riguardato la formazione della miscela per il motore Rotax 650cc nella versione allestita per il banco prova. Altre simulazioni sono state effettuate al fine di prevedere gli effetti di dettagli geometrici del sistema di iniezione. Ulteriori simulazioni hanno consentito di studiare il comportamento di differenti profili di valvola e sede valvola adatti a realizzare l'iniezione

A time tracking system for EU-funded INFN projects

The aim of this work is to describe the structure of a web application to support EU-funded INFN projects in the process of time tracking and reporting. The system has been designed in the early 2010s, following the growth of the EU-funded projects and the need for a framework to handle consistent reports according to the EU guidelines. One objective of this project is to guarantee and certify a valid distribution of hours declared on EU projects by INFN personnel in consistency with the official INFN clocking system, the scientific project management system and, overall, any signed agreements between the INFN and the founding bodies. The system allows the project participants to declare the hours worked on their projects, detailing their distribution across days, work packages and tasks. Interfaces are provided for Project Managers and Financial Officers to manage a project and monitor its trend. This activity is also supported by the INFN Business Intelligence Service, integrated with this system, to provide comprehensive reports for the upper management

Integrating SQuARE data quality model with ISO 31000 risk management to measure and mitigate software bias

In the last decades the exponential growth of available information, together with the availability of systems able to learn the knowledge that is present in the data, has pushed towards the complete automation of many decision- making processes in public and private organizations. This circumstance is posing impelling ethical and legal issues since a large number of studies and journalistic investigations showed that software-based decisions, when based on historical data, perpetuate the same prejudices and bias existing in society, resulting in a systematic and inescapable negative impact for individuals from minorities and disadvantaged groups. The problem is so relevant that the terms data bias and algorithm ethics have become familiar not only to researchers, but also to industry leaders and policy makers. In this context, we believe that the ISO SQuaRE standard, if appropriately integrated with risk management concepts and procedures from ISO 31000, can play an important role in democratizing the innovation of software-generated decisions, by making the development of this type of software systems more socially sustainable and in line with the shared values of our societies. More in details, we identified two additional measure for a quality characteristic already present in the standard (completeness) and another that extends it (balance) with the aim of highlighting information gaps or presence of bias in the training data. Those measures serve as risk level indicators to be checked with common fairness measures that indicate the level of polarization of the software classifications/predictions. The adoption of additional features with respect to the standard broadens its scope of application, while maintaining consistency and conformity. The proposed methodology aims to find correlations between quality deficiencies and algorithm decisions, thus allowing to verify and mitigate their impact

Contraception with estradiol valerate and dienogest: adherence to the method

Purpose: The aim of the study was to examine the discontinuation rate of hormonal contraception with estradiol valerate (E2V) and dienogest (DNG). Patients and methods: We collected data at the Family Planning Clinics of the Departments of Obstetrics and Gynecology of Pisa and Cagliari. We included in the analysis 354 consecutive women using oral contraceptive pills containing E2V and DNG. We analyzed the rate and the reason for discontinuation, classifying the reasons in 5 categories: 1) minor side effects, 2) adverse events, 3) other events not directly caused by the drug or conditions for which the pill could represent a risk factor, 4) no compliance with the method and 5) no further need. Results: Of the 354 women examined, 50.8% had discontinued E2V/DNG pill. Excluding women who stopped the pill because of no further need (10.5%), 27.4% discontinued because of minor side effects, 1.7% discontinued for adverse events, 9.9% because of other events not directly caused by the drug or conditions for which the pill could represent a risk factor and 1.4% because of difficulties with compliance. Irregular bleedings were the main reasons reported for discontinuation. The time to discontinuation for irregular bleedings was significantly (p<0.02) longer in adults than in adolescents and slightly but not significantly longer in women who received information about this possible effect. Conclusion: Unacceptable cycle control was the principal cause of discontinuation of pill with E2V and DNG. An appropriate information about this possible effect may improve adherence to this combined oral contraceptive

Healthcare for all in emerging countries: a preliminary investigation of facilities in Kolkata, India

Introduction. India is one of the five countries with the lowest public health spending levels: the private medical services are one of the most developed fields, meanwhile public health ones are totally inadequate with the most number of health facilities concentrated in cities, where only the 25% of the population lives. Public facilities are mainly made up of primary level and not well distributed on the territories, so they do not guarantee accessibility to a wide sample of population, who live in rural areas of India. Methodology. Starting from the analysis of three different health care levels in the Indian context, the research team developed a meta-project that considers all the current criticisms and the Indian customs through a flexible layout that responds to the healthcare needs of population. Result and Discussion. The research work is aimed to develop a meta-project that considers all the current criticisms and the Indian customs through flexible layouts that responds to the healthcare needs of population. Conclusion. The innovation of the research work is to develop the hygienic aspects, the layout and the distribution, the sub-division of the medical functions through the Indian culture and the needs of the country. The choice of lowly technologies permits to promote the usage of local materials, their maintenance and skills for creating a virtuous economic system

Modelling osteomyelitis.

BACKGROUND: This work focuses on the computational modelling of osteomyelitis, a bone pathology caused by bacteria infection (mostly Staphylococcus aureus). The infection alters the RANK/RANKL/OPG signalling dynamics that regulates osteoblasts and osteoclasts behaviour in bone remodelling, i.e. the resorption and mineralization activity. The infection rapidly leads to severe bone loss, necrosis of the affected portion, and it may even spread to other parts of the body. On the other hand, osteoporosis is not a bacterial infection but similarly is a defective bone pathology arising due to imbalances in the RANK/RANKL/OPG molecular pathway, and due to the progressive weakening of bone structure. RESULTS: Since both osteoporosis and osteomyelitis cause loss of bone mass, we focused on comparing the dynamics of these diseases by means of computational models. Firstly, we performed meta-analysis on a gene expression data of normal, osteoporotic and osteomyelitis bone conditions. We mainly focused on RANKL/OPG signalling, the TNF and TNF receptor superfamilies and the NF-kB pathway. Using information from the gene expression data we estimated parameters for a novel model of osteoporosis and of osteomyelitis. Our models could be seen as a hybrid ODE and probabilistic verification modelling framework which aims at investigating the dynamics of the effects of the infection in bone remodelling. Finally we discuss different diagnostic estimators defined by formal verification techniques, in order to assess different bone pathologies (osteopenia, osteoporosis and osteomyelitis) in an effective way. CONCLUSIONS: We present a modeling framework able to reproduce aspects of the different bone remodeling defective dynamics of osteomyelitis and osteoporosis. We report that the verification-based estimators are meaningful in the light of a feed forward between computational medicine and clinical bioinformatics.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

Sensitivity of stomatal conductance to soil moisture: Implications for tropospheric ozone

Abstract. Soil moisture and water stress play a pivotal role in regulating stomatal behaviour of plants; however, in the last decade, the role of water availability has often been neglected in atmospheric chemistry modelling studies as well as in integrated risk assessments, despite the fact that plants remove a large amount of atmospheric compounds from the lower troposphere through stomata. The main aim of this study is to evaluate, within the chemistry transport model CHIMERE, the effect of soil water limitation on stomatal conductance and assess the resulting changes in atmospheric chemistry testing various hypotheses of water uptake by plants in the rooting zone. Results highlight how dry deposition significantly declines when soil moisture is used to regulate the stomatal opening, mainly in the semi-arid environments: in particular, over Europe the amount of ozone removed by dry deposition in one year without considering any soil water limitation to stomatal conductance is about 8.5 TgO3, while using a dynamic layer that ensures that plants maximize the water uptake from soil, we found a reduction of about 10 % in the amount of ozone removed by dry deposition (∼ 7.7 TgO3). Although dry deposition occurs from the top of canopy to ground level, it affects the concentration of gases remaining in the lower atmosphere, with a significant impact on ozone concentration (up to 4 ppb) extending from the surface to the upper troposphere (up to 650 hPa). Our results shed light on the importance of improving the parameterizations of processes occurring at plant level (i.e. from the soil to the canopy) as they have significant implications for concentration of gases in the lower troposphere and resulting risk assessments for vegetation or human health

Flux-based ozone risk assessment for a plant injury index (pii) in three european cool-temperate deciduous tree species

This study investigated visible foliar ozone (O3) injury in three deciduous tree species with different growth patterns (indeterminate, Alnus glutinosa (L.) Gaertn.; intermediate, Sorbus aucuparia L.; and determinate, Vaccinium myrtillus L.) from May to August 2018. Ozone effects on the timing of injury onset and a plant injury index (PII) were investigated using two O3 indices, i.e., AOT40 (accumulative O3 exposure over 40 ppb during daylight hours) and PODY (phytotoxic O3 dose above a flux threshold of Y nmol m−2 s−1). A new parameterization for PODY estimation was developed for each species. Measurements were carried out in an O3 free-air controlled exposure (FACE) experiment with three levels of O3 treatment (ambient, AA; 1.5 × AA; and 2.0 × AA). Injury onset was found in May at 2.0 × AA in all three species and the timing of the onset was determined by the amount of stomatal O3 uptake. It required 4.0 mmol m−2 POD0 and 5.5 to 9.0 ppm·h AOT40. As a result, A. glutinosa with high stomatal conductance (gs) showed the earliest emergence of O3 visible injury among the three species. After the onset, O3 visible injury expanded to the plant level as confirmed by increased PII values. In A. glutinosa with indeterminate growth pattern, a new leaf formation alleviated the expansion of O3 visible injury at the plant level. V. myrtillus showed a dramatic increase of PII from June to July due to higher sensitivity to O3 in its flowering and fruiting stage. Ozone impacts on PII were better explained by the flux-based index, PODY, as compared with the exposure-based index, AOT40. The critical levels (CLs) corresponding to PII = 5 were 8.1 mmol m−2 POD7 in A. glutinosa, 22 mmol m−2 POD0 in S. aucuparia, and 5.8 mmol m−2 POD1 in V. myrtillus. The results highlight that the CLs for PII are species-specific. Establishing species-specific O3 flux-effect relationships should be key for a quantitative O3 risk assessment

In vitro mechanical stimulation to reproduce the pathological hallmarks of human cardiac fibrosis on a beating chip and predict the efficacy of drugs and advanced therapies

Cardiac fibrosis is one of the main causes of heart failure, significantly contributing to mortality. The discovery and development of effective therapies able to heal fibrotic pathological symptoms thus remain of paramount importance. Micro-physiological systems (MPS) are recently introduced as promising platforms able to accelerate this finding. Here a 3D in vitro model of human cardiac fibrosis, named uScar, is developed by imposing a cyclic mechanical stimulation to human atrial cardiac fibroblasts (AHCFs) cultured in a 3D beating heart-on-chip and exploited to screen drugs and advanced therapeutics. The sole provision of a cyclic 10% uniaxial strain at 1 Hz to the microtissues is sufficient to trigger fibrotic traits, inducing a consistent fibroblast-to-myofibroblast transition and an enhanced expression and production of extracellular matrix (ECM) proteins. Standard of care anti-fibrotic drugs (i.e., Pirfenidone and Tranilast) are confirmed to be efficient in preventing the onset of fibrotic traits in uScar. Conversely, the mechanical stimulation applied to the microtissues limit the ability of a miRNA therapy to directly reprogram fibroblasts into cardiomyocytes (CMs), despite its proved efficacy in 2D models. Such results demonstrate the importance of incorporating in vivo-like stimulations to generate more representative 3D in vitro models able to predict the efficacy of therapies in patients