How to use statistics to survive

Purpose We want to share a true story with an happy end that occurred in a research Library at the University of Bologna. The lesson we learned is that to achieve an international standard for quality may be misunderstood; and that how to use statistics related to a single library may become a battle field if the two “players” - in this case, a research library and the Central Library System - look at it from different perspectives. Design/methodology/approach The advocacy strategy adopted by the library is based on a range of statistics to corroborate a wider meaning for “efficacy “and “efficiency”. The user’s point of view, satisfaction statements, observations and explored behavior – as known via the library quality system– are used, in parallel to the dimension of time in delivery services. Findings By means of the ISO 9001 quality system, the research library has been stimulated to measure different aspects of the library services, that could be of some interest if applied also at the Central Library System level. The reason behind the selection of such indicators is related to the main goal of the quality certification, that is to raise users’ satisfaction. Practical implications The paper presents also an example of good practice. The central library system at the University of Ferrara has build a set of indicators that could meet the requirements of three different schemes, all of which were of great importance for the library system management: a) the ISO 9001 requirements; b) the university overall KPI (Key Performance Indicators) scheme, aiming at defining a set of indicators that could measure and supervise all the main processes in terms of efficiency and efficacy of offices/libraries as well as of the staff; c)the national context. Originality/value Very often small or medium sized libraries strive to demonstrate their usefulness, efficacy and efficiency. Very often the users perceptions of the library services are less taken in consideration than quantitative measures on services outputs. This paper is a good example of a “survival” strategy of success

Tear ferning test and pathological effects on ocular surface before and after topical cyclosporine in vernal keratoconjunctivitis patients

Background: Vernal keratoconjunctivitis (VKC) is a rare ocular surface inflammatory disease that affects mainly boys in the first decade of life. Clinical observations show that it generally regresses spontaneously with the onset of puberty, but therapeutic measures must be taken before then to control the course of the disease. Purpose: To evaluate the role of the lacrimal mucous component in VKC patients and compare tear ferning test (TFT) modifications, MUC5AC levels in tears, and density of conjunctival goblet cells to clinical characteristics before and after treatment with cyclosporine A (CY) in eye drops. Methods: Forty-seven patients affected by VKC and 30 healthy subjects aged between 3 and 16 years of life were enrolled. All individuals were submitted to complete eye examination and skin prick test (SPT) for the most common allergens. Then, they were subjected to collection of the tears and to impression cytology to evaluate TFT, MUC5AC levels, and conjunctival goblet cell density, before and after treatment with CY in eye drops. Results: Comparing the VKC group vs. the control group at baseline, a significant alteration in the degree of the ferns was found, indicating a pathological condition of the lacrimal mucous layer. In addition, an increased number of goblet cells were observed in the patients. The concentration of lacrimal secretory mucins (MUC5AC) did not show significant differences between the 2 groups. Patients treated with CY have reported improvements of some signs and symptoms of disease activity, including TFT, and a tendency of conjunctival goblet cell density to normalise. Conclusions: The results obtained demonstrated for the first time a significant alteration of the lacrimal mucin component evaluated in the VKC group, and an improvement of the latter after CY therapy

Ketogenic diet modulates NAD\u3csup\u3e+\u3c/sup\u3e-dependent enzymes and reduces DNA damage in hippocampus

© 2018 Elamin, Ruskin, Masino and Sacchetti. The ketogenic diet’s (KD) anti-seizure effects have long been documented. Recently, its therapeutic potential in multiple neurodegenerative and neurodevelopmental disorders has emerged. Yet experimental evidence for a fundamental mechanism underlying beneficial effects across numerous diseases remains lacking. We previously showed that feeding rats a KD produced an early (within 2 days) and persistent elevation of hippocampal nicotinamide adenine dinucleotide+ (NAD+), an essential metabolic coenzyme and signaling molecule. NAD+ is a marker of cellular health and a substrate for enzymes implicated in longevity and DNA damage repair such as sirtuins and poly-ADP ribose polymerase-1 (PARP-1). As a result, activation of NAD+-dependent enzymes’ downstream pathways could be the origin of KD’s broad beneficial effects. Here rats were fed ad libitum regular chow or KD for 2 days or 3 weeks and the levels of hippocampal sirtuins, PARP-1, and the oxidative DNA damage marker 8-hydroxy-2’-deoxyguanosine were quantified. We found a significant immediate and persistent increase in the collective activity of nuclear sirtuin enzymes, and a significant augmentation of Sirt1 mRNA at 2 days. Levels of PARP-1 and 8-hydroxy-2’- deoxyguanosine decreased after 2 days of treatment and further declined at 3 weeks. Our data show that a KD can rapidly modulate energy metabolism by acting on NAD+-dependent enzymes and their downstream pathways. Thus, therapy with a KD can potentially enhance brain health and increase overall healthspan via NAD+- related mechanisms that render cells more resilient against DNA damage and a host of metabolic, epileptic, neurodegenerative, or neurodevelopmental insults

Correlation energy, pair-distribution functions and static structure factors of jellium

We discuss and clarify a simple and accurate interpolation scheme for the spin-resolved electron static structure factor (and corresponding pair correlation function) of the 3D unpolarized homogeneous electron gas which, along with some analytic properties of the spin-resolved pair-correlation functions, we have just published. We compare our results with the very recent spin-resolved scheme by Schmidt et al., and focus our attention on the spin-resolved correlation energies and the high-density limit of the correlation functions.Comment: 8 pages, 3 figures. Proceedings of the conference on Statistical Mechanics and Strongly Correlated Systems (Bachelet, Parisi & Vulpiani Eds.) to appear as a special issue of Physica A (Elsevier, Amsterdam 2000

PTP4A1 promotes TGFβ signaling and fibrosis in systemic sclerosis.

Systemic sclerosis (SSc) is an autoimmune disease characterized by fibrosis of skin and internal organs. Protein tyrosine phosphatases have received little attention in the study of SSc or fibrosis. Here, we show that the tyrosine phosphatase PTP4A1 is highly expressed in fibroblasts from patients with SSc. PTP4A1 and its close homolog PTP4A2 are critical promoters of TGFβ signaling in primary dermal fibroblasts and of bleomycin-induced fibrosis in vivo. PTP4A1 promotes TGFβ signaling in human fibroblasts through enhancement of ERK activity, which stimulates SMAD3 expression and nuclear translocation. Upstream from ERK, we show that PTP4A1 directly interacts with SRC and inhibits SRC basal activation independently of its phosphatase activity. Unexpectedly, PTP4A2 minimally interacts with SRC and does not promote the SRC-ERK-SMAD3 pathway. Thus, in addition to defining PTP4A1 as a molecule of interest for TGFβ-dependent fibrosis, our study provides information regarding the functional specificity of different members of the PTP4A subclass of phosphatases

Characterization of natural clays from italian deposits with focus on elemental composition and exchange estimated by edx analysis: potential pharmaceutical and cosmetic uses

Purification processes performed on natural clays to select specific clay minerals are complex and expensive and can lead to over-exploitation of some deposits. The present study aimed to examine physicochemical (mineralogy, morphology, size, surface charge, chemical composition, cation exchange capacity [CEC], and pH) and hydration (swelling, wettability, water sorption, and rheological behavior) properties of three native clays from Italian deposits for potential pharmaceutical and cosmetic uses due to the presence of phyllosilicate minerals. Particular emphasis was placed on energy dispersive X-ray (EDX) microanalysis coupled with the ‘cesium method’ to assay clay elemental composition and CEC. One bentonite of volcanic origin (BNT) and two kaolins, one of hydrothermal origin (K-H) and another of lacustrine-fluvial origin (K-L), were evaluated in comparison with a commercial, purified bentonite. The CEC assay revealed the complete substitution of exchangeable cations (Na+ and Ca2+) by Cs+ in BNT samples and CEC values consistent with those of typical smectites (100.64 7.33 meq/100 g). For kaolins, partial substitution of Na+ cations occurred only in the K-L samples because of the interstratified mineral component which has small CEC values (11.13 5.46 meq/100 g for the K-H sample and 14.75 6.58 meq/100 g for the K-L sample). The degree of isomorphous substitution of Al3+ by Mg2+ affected the hydration properties of BNT in terms of swelling, water sorption, and rheology, whereas both of the poorly expandable kaolins exhibited significant water-adsorption properties. The EDX microanalysis has proved to be of considerable interest in terms of providing more information about clay properties in comparison with other commonly used methods and to identify the role played by both chemical and mineralogical composition of natural clays for their appropriate use in pharmaceutical and cosmetic fields

Amaretti senza amido e farina come ingredienti in sistemi alimentari ad alta umidità

Riassunto Due tipologie di amaretti sono state ottenute utilizzando diverse formulazioni, una contenente amido come strutturante (VR) ed una preparata in accordo all’attuale normativa (NR) che non ammette la presenza di amido nella formulazione. Gli amaretti formulati con la nuova ricetta (NR) hanno mostrato valori di densità e durezza minori di quelli ottenuti con la vecchia ricetta ma una friabilità non significativamente differente. Le cinetiche di reidratazione e le caratteristiche meccaniche dei prodotti a diversi livelli di idratazione sono state studiate a seguito di immersione degli amaretti in soluzione di saccarosio a 25° C e di contatto su gelato a -15° C. In soluzione di saccarosio il prodotto NR ha mostrato una maggiore capacità di reidratazione ed una minore durezza e friabilità a tutti i livelli di idratazione. Nella reidratazione a -15° C su gelato il prodotto NR ha mostrato un acquisto di umidità non differente rispetto a VR ed una minore riduzione della friabilità, pur presentando una consistenza minore di VR a tutti i livelli di idratazione. La sostituzione dell’amido nella formulazione di biscotti modifica le proprietà fisiche e meccaniche del prodotto ma può permette migliori performance in termini di friabilità a bassi livelli di umidità.Two types of ‘amaretti’ biscuits were produced with different formulations: one containing starch as a structuring agent (VR) and the other modified according to the current Italian legislation (NR) which does not permit the use of starch in the ‘amaretti’formulation. Amaretti formulated with the new recipe (NR) showed lower density and hardness than VR amaretti but not a significantly different friability value. Rehydration experiments in sucrose solution (25° C) and on ice-cream (-15° C) were carried out and rehydration kinetics and texture changes upon rehydration were evaluated. During rehydration in sucrose solution, the NR product showed a higher moisture uptake than VR and lower hardness and friability values at different hydration levels. When rehydration was carried out on ice-cream (at -15° C), NR showed a lower friability reduction than VR and a not different moisture uptake but it still showed lower hardness values than the latter at each hydration level. The replacement of starch in biscuits formulation affects the physical and mechanical properties of the products but could permit better performance in terms of friability retention at low moisture content

Biological activity of new bioactive steroids deriving from biotransformation of cortisone

Cortisone is a metabolite belonging to the corticosteroid class that is used pharmaceutically directly as a drug or prodrug. In addition to its large consumption, its use is linked to several side effects, so pharmaceutical research aims to develop effective drugs with low or no side effects, alternative compounds to cortisone are part of an active investment in ongoing research on drug discovery. Since biotransformation can be considered a source of new molecules with potential therapeutic use, the present work focuses on a preliminary in vitro study aimed at evaluating the mutagenic, anti-inflammatory, antioxidant and neuroprotective activity of SCA and SCB molecules obtained from the biotransformation of cortisone using Rh. Rhodnii strain DSM 43960. The results obtained are very encouraging due to the safety of biotransformed compounds with reference to genotoxicity checked by Ames test, to the very high antioxidant capacity and to the anti-inflammatory activity. In fact, thecompounds inhibited both the TNF alpha-stimulated expression and secretion of NFkB target cytokines, and COX activity, and can activate the glucocorticoid receptor. Finally SCA and SCB exhibited neuroprotective properties

Mechanisms of endothelial cell dysfunction in cystic fibrosis

Although cystic fibrosis (CF) patients exhibit signs of endothelial perturbation, the functions of the cystic fibrosis conductance regulator (CFTR) in vascular endothelial cells (EC) are poorly defined. We sought to uncover biological activities of endothelial CFTR, relevant for vascular homeostasis and inflammation. We examined cells from human umbilical cords (HUVEC) and pulmonary artery isolated from non-cystic fibrosis (PAEC) and CF human lungs (CF-PAEC), under static conditions or physiological shear. CFTR activity, clearly detected in HUVEC and PAEC, was markedly reduced in CF-PAEC. CFTR blockade increased endothelial permeability to macromolecules and reduced trans‑endothelial electrical resistance (TEER). Consistent with this, CF-PAEC displayed lower TEER compared to PAEC. Under shear, CFTR blockade reduced VE-cadherin and p120 catenin membrane expression and triggered the formation of paxillin- and vinculin-enriched membrane blebs that evolved in shrinking of the cell body and disruption of cell-cell contacts. These changes were accompanied by enhanced release of microvesicles, which displayed reduced capability to stimulate proliferation in recipient EC. CFTR blockade also suppressed insulin-induced NO generation by EC, likely by inhibiting eNOS and AKT phosphorylation, whereas it enhanced IL-8 release. Remarkably, phosphodiesterase inhibitors in combination with a β2 adrenergic receptor agonist corrected functional and morphological changes triggered by CFTR dysfunction in EC. Our results uncover regulatory functions of CFTR in EC, suggesting a physiological role of CFTR in the maintenance EC homeostasis and its involvement in pathogenetic aspects of CF. Moreover, our findings open avenues for novel pharmacology to control endothelial dysfunction and its consequences in CF