Dataset about substitution of Mg2+ cofactor with Ca2+ disrupt positive cooperativity in F1FO-ATP(hydrol)ase catalysis

This dataset contains data from the evaluation of the hydrolytic activity of mitochondrial F1FO-ATPase, isolated from porcine heart, activated by the natural cofactor Mg²⁺ or replaced by Ca²⁺. The aim of the research was to investigate how replacing the physiological cofactor Mg²⁺ with Ca²⁺ affects the catalysis, cooperativity, and mechanochemical functionality of mitochondrial F1FO-ATPase, in order to understand the molecular mechanisms that determine the transition of the enzyme from its physiological role as an ATP synthase (the "enzyme of life") to its pathological function as a component of the mitochondrial permeability transition pore (mPTP, "enzyme of death"). "F_ATPase_cooperativity_Dataset.zip" contains data on the activity of the Mg2+-dependent F1FO-ATPase, which is more efficient than the Ca2+-dependent activity ("curve_MgATPase.csv" and "curve_CaATPase.csv"). Evaluation of the cooperativity of the Mg²⁺- and Ca²⁺-dependent F1FO-ATPase revealed a differential cooperative effect on the ATP substrate, depending on the specific cofactor involved. A loss of cooperativity is evident when Mg²⁺ ("nHi_MgATPase.csv") is replaced with Ca²⁺ ("nHi_CaATPase.csv"). Data from experiments investigating the kinetics of the cofactors interacting with the enzyme are reported in "Inhibition mechanism.csv", which show a reduction in kinetic parameters in presence of Ca2+, suggesting uncompetitive inhibition

Dataset about modulation of mitochondrial bioenergetics by phytochemicals: effect on F1FO-ATPase, mitochondrial respiration and permeability transition pore

This dataset contains data from the evaluation of the effect of S-allyl cysteine (SAC), Benzyl isothiocyanate (BITC), and Epicatechin (EPC) on mitochondria isolated from porcine hearts. The aim of the research was to verify the modulatory effect of the various molecules on mitochondrial bioenergetics through structure/activity correlation studies of the mitochondrial enzyme F1FO-ATPase, understand the involvement of the redox state on the induced effects, verify the kinetic parameters, and evaluate the effect on the phenomenon of the opening of the mitochondrial permeability transition pore (mPTP), involved in regulated cell death processes. The results, reported in “SAC_BITC_EPC_mitochondria_Dataset.xlsx”, show that it was possible to calculate Hill coefficients (nHi) for the hydrolytic activity of the Mg2+-activated enzyme in the absence and presence of BITC (“nHi_BITC” sheet) and SAC (“nHi_SAC” sheet), and for the Ca2+-activated enzyme in the absence and presence of BITC+ dithiothreitol (DTT) (“nHi_BITC_DTT_Ca_sub; “nHi_BITC_DTT_Ca_cof “ sheets). SAC showed an activating effect on Mg2+-activated F1FO-ATPase (“SAC_MgATPase” sheet) but not when activated by Ca2+ (“SAC_CaATPase” sheet) and its activating effect was lost in the presence of DTT (“SAC_MgATPaseDTT” sheet). BITC showed an inhibit effect on Mg2+-activated F1FO-ATPase (“BITC_MgATPase” sheet) but not when activated by Ca2+ (“BITC_CaATPase” sheet). In presence of DTT, the effect of BITC on Mg2+-activated enzyme was not modulated (“BITC_MgATPaseDTT” sheet) while it was enhanced on Ca2+-activated enzyme (“BITC_CaATPaseDTT” sheet). EPC did not alter Mg2+- (“EPC_MgATPase” sheet) or Ca2+-activated F1FO-ATPase (“EPC_CaATPase” sheet) in any way. SAC showed competitive activation for the cofactor Mg2+ (“Kinetics SAC_cofactorMg” sheet) and uncompetitive activation for the substrate ATP (“Kinetics SAC_substrateATP” sheet). BITC inhibited with a mixed-type mechanism towards the substrate (“Kinetics BITC_substrateATP” sheet) and the cofactor Mg2+ (“Kinetics BITC_cofactorMg” sheet). The inhibition of BITC on Ca2+-activated F1FO-ATPase exerted in the presence of DTT was found to be of mixed type towards the substrate ATP (“Kinetics BITC_substrate_Ca” sheet) and cofactor Ca2+ (“Kinetics BITC_cofactorCa” sheet). SAC and EPC did not alter mitochondrial respiration as assessed by Complex I of the electron transport chain (NADH-O2 oxidoreductase activity) and Complex II (SUCC-O2 oxidoreductase activity) (“SAC NADH-SUCC_O2”; “EPC NADH-SUCC_O2” sheets). BITC also inhibited respiration at high concentrations (“BITC NADH-SUCC_O2” sheet). The modulatory effect of SAC, BITC and EPC was also evaluated on CRC (“CRC” sheet), an index of mPTP opening

Data about the role of RXRγ in T3-mediated oligodendrocyte precursor cell differentiation

This dataset contains data describing the effect of the knock-out of the Rxrg gene in neral stem cell (NSC)-derived oligodendrocyte precursor cells (OPCs) differentiation and maturation. Data has been obtained by transcriptomic analysis using RNAseq in cells isolated by WT and Rxrg-/- animals cultured as indifferentiated precursors and 24 hours after the differentiation induction by triiodotironine (T3) exposure, mimiking the physiologic differentiation/maturation molecular machinery. RNAseq data has been validated using morphological analysis. Molecular tools has been used to inhibit (cyclopamine, GANT61) or activate (SAG, purmorphamine) the sonic hadgehog (SHH) pathway in differentiating WT and Rxrg-/- OPC cultures. Specific markers (NG2, MBP) have been used to quantify the differentiation/maturation of the OPC culture, measuring the percentage of precursors and mature oligodendrocytes

Lo spettacolo claustrale: musica, spazio, liturgia nei monasteri femminili palermitani

Fra i secoli XVII e XVIII il contributo dei monasteri femminili al panorama musicale palermitano si caratterizzava per la promozione di eventi che per magnificenza e splendore attiravano un gran numero di fedeli. Le liturgie si distinguevanoper sfarzo e solennità, nel quadro di sontuosi apparati, di sfavillanti luminarie, di cibi e decorazioni, nonché delle musiche con cui le monache impreziosivano le celebrazioni. Per le feste più importanti le istituzioni si avvalevano di musici esterni, affiliati alla Cattedrale o alla Cappella reale; tuttavia, in quelle stesse occasioni le monache cantavano e suonavano in prima persona. La considerazione degli spazi claustrali giocava il suo ruolo nella scelta delle soluzioni performative, ricoprendo una funzione rilevante nell’organizzazione delle celebrazioni, oltre che nel rinsaldare i legami con il territorio; tutto questo a dispetto delle prescrizioni ufficiali, che tentavano inutilmente di limitare l’impiego della musica. L’analisi delle costituzioni, così come di cronache e libretti, serve come punto di partenza per comprendere le usanze sonore dei monasteri palermitani, confermando come la presenza della musica fosse in grado di conferire rilievo a liturgie sia locali sia di più ampia diffusione

The Capital’s Revolutions

The volume proposes adopting the perspective of the “revolutions of capital” as a lens through which to reinterpret, in historical and political-philosophical terms, the shaping of the current digital revolution. The entry point for addressing the theme is the urban, an analytical prism for observing how capitalist revolutions have historically taken shape through the upheaval of a constellation of factors. By tracing genealogically the historical sequences that lead us today to speak of an ongoing “Fourth Industrial Revolution,” the volume shows, through seven ur-ban case studies, that a revolution of capital—far from being exhausted in a mere technological transformation (be it the steam engine, the railway, the Fordist factory, or Artificial Intelligence)—rather entails the redefinition of social relations, of the state form, and of powers within the world market. Lisbon, London, Berlin, Paris, Bologna, Barcelona, and Tallinn are examined here in order to retrace, from the late eighteenth century to the present day, the changing assemblages just mentioned, through an approach defined as “trans-urban.” This allows both for a longue durée perspective and for an appreciation of the discontinuities and ruptures that have marked this historical development

Echocardiography parameters of healthy neonatal foals

This dataset includes echocardiographic measurements from healthy Standardbred neonatal foals, comprising age and body weight at examination and measured echocardiographic parameters. Three files are provided: one containing all measured and calculated parameters, and two containing only measured parameters used for inter- and intra-observer variability analyses

The Innovation Long-Run Risk Component

This paper provides robust empirical evidence that shocks to aggregate Research and Development (R&D) have persistent effects on macroeconomic dynamics and represent a significant risk for investors, as predicted by the ‘long-run risk’ literature. The analysis focuses on a single variable, ‘effective R&D’, which captures the entire contribution of R&D to productivity growth, flexibly accounting for knowledge spillovers and product proliferation effects. Deviations of effective R&D from its equilibrium level can be empirically identified leveraging the error correction term in the cointegration relationship among R&D, total factor productivity, and the labor force. In US data, structural effective R&D shocks affect productivity and consumption growth rates beyond business cycle horizons and are associated with a significant risk premium in a cross section of stock and bond portfolios (around 2% annually), with cash-flow sensitivities proving a key determinant

TheGAME. Greece. 2024. Valeria Raimondi. Interviews and Reports. V1

This dataset containes interviews collected by Valeria Raimondi during her fieldwork in Greece within the Horizon Europe ERC project “TheGAME” in 2023 and 2024

Data on bearing walnut orchard soil and plant nutritional status in response to different amendments supply

This dataset contains data from a bearing walnut orchard fertilized with mineral and organic amendments and compared to non-fertilized control. The data refers to plant growth and nutritional status as well as on soil fertility over a period of two year

TheGAME. Trieste. 2023. Interviews

This dataset contains interviews from Trieste collected within the Horizon Europe ERC project “TheGAME” in 2023