Nonparametric Regression Estimation for Circular Data

[Abstract] Non-parametric regression with a circular response variable and a unidimensional linear regressor is a topic which was discussed in the literature. In this work, we extend the results to the case of multivariate linear explanatory variables. Nonparametric procedures to estimate the circular regression function are formulated. A simulation study is carried out to study the sample performance of the proposed estimators.Ministerio de Economía y Competitividad; MTM2016-76969-PMinisterio de Economía y Competitividad; MTM2017-82724-RXunta de Galicia; ED481A-2017/361Grupos de Referencia Competitiva; ED431C-2016-015Centro Singular de Investigación de Galicia; ED431G/0

Nonparametric multiple regression estimation for circular response

Versión final aceptada de: https://doi.org/10.1007/s11749-020-00736-wThis version of the article has been accepted for publication, after peer review and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect postacceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s11749-020-00736-wNonparametric estimators of a regression function with circular response and -valued predictor are considered in this work. Local polynomial estimators are proposed and studied. Expressions for the asymptotic conditional bias and variance of these estimators are derived, and some guidelines to select asymptotically optimal local bandwidth matrices are also provided. The finite sample behavior of the proposed estimators is assessed through simulations, and their performance is also illustrated with a real data set.The authors acknowledge the support from the Xunta de Galicia Grant ED481A-2017/361 and the European Union (European Social Fund—ESF). This research has been partially supported by MINECO Grants MTM2016-76969-P and MTM2017-82724-R, and by the Xunta de Galicia (Grupo de Referencia Competitiva ED431C-2017-38, and Centro de Investigación de Galicia “CITIC” ED431G 2019/01), all of them through the ERDF. The authors thank Prof. Felicita Scapini and his research team who kindly provided the sand hoppers data that are used in this work. Data were collected within the Project ERB ICI8-CT98-0270 from the European Commission, Directorate General XII Science. The authors also thank two anonymous referees for numerous useful comments that significantly improved this article.Xunta de Galicia; ED481A-2017/361Xunta de Galicia; ED431C-2017-38Xunta de Galicia; ED431G 2019/0

Satellitome analysis of Rhodnius prolixus, one of the main Chagas disease vector species

The triatomine Rhodnius prolixus is the main vector of Chagas disease in countries such as Colombia and Venezuela, and the first kissing bug whose genome has been sequenced and assembled. In the repetitive genome fraction (repeatome) of this species, the transposable elements represented 19% of R. prolixus genome, being mostly DNA transposon (Class II elements). However, scarce information has been published regarding another important repeated DNA fraction, the satellite DNA (satDNA), or satellitome. Here, we offer, for the first time, extended data about satellite DNA families in the R. prolixus genome using bioinformatics pipeline based on low-coverage sequencing data. The satellitome of R. prolixus represents 8% of the total genome and it is composed by 39 satDNA families, including four satDNA families that are shared with Triatoma infestans, as well as telomeric (TTAGG)n and (GATA)n repeats, also present in the T. infestans genome. Only three of them exceed 1% of the genome. Chromosomal hybridization with these satDNA probes showed dispersed signals over the euchromatin of all chromosomes, both in autosomes and sex chromosomes. Moreover, clustering analysis revealed that most abundant satDNA families configured several superclusters, indicating that R. prolixus satellitome is complex and that the four most abundant satDNA families are composed by different subfamilies. Additionally, transcription of satDNA families was analyzed in different tissues, showing that 33 out of 39 satDNA families are transcribed in four different patterns of expression across samples

Numerical and experimental analyses of biocomposites reinforced with natural fibres

n the last decades the biocomposites have been widely used in the construction, automobile and aerospace industries. Not only the interface transition zone (ITZ) but also the heterogeneity of natural fibres affects the mechanical behaviour of these composites. This work focuses on the numerical and experimental analyses of a polymeric composite fabricated with epoxy resin and unidirectional sisal and banana fibres. A three-dimensional model was set to analyze the composites using the elastic properties of the individual phases. In addition, a two-dimensional model was set taking into account the effective composite properties obtained by micromechanical models. A tensile testing was performed to validate the numerical analyses and evaluating the interface condition of the constitutive phases

Comparative repeatome analysis on Triatoma infestans Andean and Non-Andean lineages, main vector of Chagas disease

Triatoma infestans is the most important Chagas disease vector in South America. Two main evolutionary lineages, named Andean and non-Andean, have been recognized by geographical distribution, phenetic and genetic characteristics. One of the main differences is the genomic size, varying over 30% in their haploid DNA content. Here we realize a genome wide analysis to compare the repetitive genome fraction (repeatome) between both lineages in order to identify the main repetitive DNA changes occurred during T. infestans differentiation process. RepeatExplorer analysis using Illumina reads showed that both lineages exhibit the same amount of non-repeat sequences, and that satellite DNA is by far the major component of repetitive DNA and the main responsible for the genome size differentiation between both lineages. We characterize 42 satellite DNA families, which are virtually all present in both lineages but with different amount in each lineage. Furthermore, chromosomal location of satellite DNA by fluorescence in situ hybridization showed that genomic variations in T. infestans are mainly due to satellite DNA families located on the heterochromatic regions. The results also show that many satDNA families are located on the euchromatic regions of the chromosomes

Influence of Displacements on Calculus of the Longitudinal Modulus of Elasticity of Pinus Caribaea Structural Round Timber Beams

Round timber shows great potential for use as a building material, having the advantage of not being proc-essed, such as the sawn wood. In Brazil, the normative standards that deal with the existing round timber elements are mainly headed for the pole market, being in force for at least twenty years without technical review, recommending char-acterization of this material by destructive methods, using small specimens with no defects and a cantilever beam structural model[2]. This paper aims to determine the longitudinal modulus of elasticity of Pinus caribaea structural round timber beams using static three-point bending test under physical and geometrical linearity conditions (non-destructive methodol-ogy) which evaluates the effect of the L/200 and L/300 displacement measurements for this purpose[6]. The results achieved by the confidence interval show the statistical equivalence between the values of the modulus of elasticity, being possible in this case, the use of both limits in displacement measurements

Seismic Monitoring Tool for Tidal-Forced Aquifer Level Changes in the Río de la Plata Coastal Plain, Argentina

Ambient seismic noise has gained extensive applications in seismology and plays a pivotal role in environmental seismic studies. This study focuses on the Río de la Plata Coastal Plain, employing the horizontal-to-vertical spectral ratio (HVSR) method on ambient seismic noise records to analyze subsurface dynamics. The region’s hydrogeology is complex, featuring partially interconnected coastal aquifers. The HVSR analysis reveals two peaks, with P0 associated with the sediment-basement interface and P1 linked to a shallower stratigraphic discontinuity. Temporal analysis of P1 highlights cyclical patterns correlated with estuarine levels, suggesting a relationship between variations in seismic velocities and tidal dynamics. Comparisons with aquifer data support the hypothesis that tidal variations influence subsurface mechanical properties, impacting the HVSR function. The study hints at the potential of ambient seismic noise analysis as a non-invasive and cost-effective method for studying coastal aquifers and understanding groundwater dynamics. Ongoing research aims to further explore these relationships for enhanced groundwater resource management

Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)

BACKGROUND Panstrongylus rufotuberculatus (Hemiptera-Reduviidae) is a triatomine species with a wide geographic distribution and a broad phenotypic variability. In some countries, this species is found infesting and colonising domiciliary ecotopes representing an epidemiological risk factor as a vector of Trypanosoma cruzi, etiological agent of Chagas disease. In spite of this, little is known about P. rufotuberculatus genetic diversity. METHODS Cytogenetic studies and DNA sequence analyses of one nuclear (ITS-2) and two mitochondrial DNA sequences (cyt b and coI) were carried out in P. rufotuberculatus individuals collected in Bolivia, Colombia, Ecuador and Mexico. Moreover, a geometric morphometrics study was applied to Bolivian, Colombian, Ecuadorian and French Guiana samples. OBJECTIVES To explore the genetic and phenetic diversity of P. rufotuberculatus from different countries, combining chromosomal studies, DNA sequence analyses and geometric morphometric comparisons. FINDINGS We found two chromosomal groups differentiated by the number of X chromosomes and the chromosomal position of the ribosomal DNA clusters. In concordance, two main morphometric profiles were detected, clearly separating the Bolivian sample from the other ones. Phylogenetic DNA analyses showed that both chromosomal groups were closely related to each other and clearly separated from the remaining Panstrongylus species. High nucleotide divergence of cyt b and coI fragments were observed among P. rufotuberculatus samples from Bolivia, Colombia, Ecuador and Mexico (Kimura 2-parameter distances higher than 9%). MAIN CONCLUSIONS Chromosomal and molecular analyses supported that the two chromosomal groups could represent different closely related species. We propose that Bolivian individuals constitute a new Panstrongylus species, being necessary a detailed morphological study for its formal description. The clear morphometric discrimination based on the wing venation pattern suggests such morphological description might be conclusive.MEC-DICYT: II/FVF/2019/054CSIC: No. 16

Biological attributes of the kissing bug Triatoma rubrofasciata from Vietnam

Background Triatoma rubrofasciata is the only kissing bug species distributed globally. In the Americas, this species transmits the parasite Trypanosoma cruzi, responsible for Chagas disease. The presence of T. rubrofasciata in several Asian countries has greatly increased recently. In Vietnam, it is found in large numbers, closely associated with human environments. Although T. rubrofasciata from Asia is not infected with Tryp. cruzi, it carries other parasites such as Trypanosoma lewisi and Trypanosoma conorhini. Reports of bites by T. rubrofasciata have increased significantly in several places of Vietnam, becoming a public health problem as it produces severe anaphylactic reactions. Methods Specimens of T. rubrofasciata were collected from seven provinces in central Vietnam. We analyzed different biological attributes (life-cycle, starvation resistance, feeding and reproductive capacities) and genetic characteristics (chromosomes and DNA sequences) of T. rubrofasciata from Vietnam and compared them with Brazilian specimens. Natural infection with Tryp. conorhini and Tryp. lewisi were analyzed in a sample of 100 collected insects. Results Species identification of T. rubrofasciata from central Vietnam was corroborated by genetic markers. Cytogenetic analyses showed that T. rubrofasciata from central Vietnam share the same chromosomal characteristics with individuals from Brazil and Hanoi. DNA sequence analyses of a mitochondrial cytochrome b gene fragment showed little variation between Old and New World specimens. Our study sample, compared with Brazilian individuals, showed a higher survival capacity revealed by a higher hatching rate (98% compared with 80.5%), a larger amount of blood taken in single meal and long-term starvation resistance. Furthermore, this species had a high natural rate of infection with Tryp. conorhini (46%) and Tryp. lewisi (27%). Conclusions For T. rubrofasciata of Vietnam, a high rate of fecundity throughout the year, a high capacity for starvation, and its occurrence in synanthropic environments of urban areas with a high availability of food sources are risk factors to be taken into account by vector control campaigns. The several allergic reactions caused by their bites and their high infection with Tryp. lewisi highlight the need to implement specific control programmes for T. rubrofasciata in Vietnam