Dados preliminares da estrutura genética - bioquimica do gado Nelore (Bos indicus) da Fazenda Canchim.

Estudo do rebanho Nelore da Fazenda Canchim, quanto aos marcadores genético - bioquímicos. levando em conta os diferentes fenótipos detectados, a população mostrou-se estar em equilíbrio.Resumo

Diseño de un Amplificador de potencia CMOS de Clase 1 para Aplicaciones Bluetooth

Un amplificador de potencia de dos etapas de clase AB en cascada operando a 2.4Ghz fue diseñado para un proceso de CMOS de 130nm para aplicaciones Bluetooth de Clase-1. El amplificador de poder fue polarizado en una clase AB para un mejor punto medio entre linealidad y eficiencia. El amplificador de potencia propuesto tiene una eficiencia total de 39.6%, una ganancia de potencia de 25dB y una salida de poder de 20dBm

A study of biochemical polymorphisms in European/Zebu dairy crossbred cattle and of their relationship with european and Zebu cattle.

Determinação da estrutura genética de um rebanho mestiços leiteiro

Estudo preliminar da relação entre marcadores genético-bioquímicos e resistência ao carrapato em gado de corte.

Relacionar os marcadores genético com a resistência ao carrapato, os animais foram classificados como sendo de alta ou baixa resistência

Marcadores genético-bioquímicos e resistência ao carrapato em bovinos.

Estudo da associação entre marcadores genético-bioquímicos e a resistência ao carrapato, foram analisados, preliminarmente, 60 animais (30 Canchim e 30 Nelore) para os sistemas albumina, transferrina e amilase em plasma sanguineo, e hemoglobina e anidrase carbônica em lisado de hemaceas

Cellophane based mini-prep method for DNA extraction from the filamentous fungus Trichoderma reesei

BACKGROUND: Methods for the extraction of DNA from filamentous fungi are frequently laborious and time consuming because most of the available protocols include maceration in liquid nitrogen after the mycelium has been grown in a liquid culture. This paper describes a new method to replace those steps, which involves the growth of the mycelium on cellophane disks overlaid on solid medium and the use of glass beads for cell wall disruption. RESULTS: Extractions carried out by this method provided approximately 2 μg of total DNA per cellophane disk for the filamentous fungus Trichoderma reesei. To assess the DNA's quality, we made a PCR (Polymerase Chain Reaction) amplification of a gene introduced by a transformation in this fungus's genome (hph gene), with successful results. We also confirmed the quality of the DNA by the use of Southern blotting to analyze the presence of the same gene, which was easily detected, resulting in a sharply defined and strong band. CONCLUSIONS: The use of this method enabled us to obtain pure DNA from Trichoderma reesei, dispensing with the laborious and time-consuming steps involved in most protocols. The DNA obtained was found to be suitable for PCR and Southern blot analyses. Another advantage of this method is the fact that several samples can be processed simultaneously, growing the fungus on multiple well cell culture plates. In addition, the absence of maceration also reduces sample handling, minimizing the risks of contamination, a particularly important factor in work involving PCR

Characterizing and modeling preferential flow using magnetic resonance imaging and multifractal theory.

bitstream/item/89956/1/Proci-07.00331.PD

Uso de ressonância magnética nuclear na caracterização de ácidos húmicos de solos sob diferentes manejos.

bitstream/CNPDIA/9813/1/PA08_96.pd

Comparative gene frequences of nucleoside phosphorylase from cattle of Bos taurus and Bos indicus derivation in Brazil.

Two nucleoside phosphorylase (NP) phenotypes were detected in 844 animals from four distinct genetic grroups of Bows indicus e Bos taurus

Exploración de la subjetividad y mecanismos de defensa en niños latentes mediante la interpretación del Cuestionario Desiderativo

El cuestionario desiderativo explora el desarrollo del psiquismo infantil, captando las sutilezas de los procesos de cambio de los diferentes momentos evolutivos. Estudia a partir de los símbolos y argumentaciones, deseos, fantasías, temores, repertorio y eficacia de defensas, capacidad simbólica, elaboración de pérdidas y grado de cohesión alcanzado por el yo. Investigaremos las respuestas más típicas en latencia, observando como cada subperíodo es atravesado por diferentes conflictos, ligados a la elaboración del Complejo de Edipo y castración y el advenimiento de la Pubertad. Entendemos que el proceso de subjetivación es continuo y se podrán explorar valores, ideales, vivencias, que darán cuenta de ciertos rasgos de personalidad y/o de carácter en formación. Emprenderemos una aproximación normativa del “Cuestionario Desiderativo” en su versión infantil en niños latentes (6 a 10) de la CABA. Hemos revisado las consignas posibles por edad, remarcando la necesidad de preguntar por aquello que desean y no por lo que les gusta. En las respuestas positivas se despliega en forma secuencial el repertorio defensivo, desde lo adaptativo a lo más regresivo. En las respuestas negativas aparece aquello que ocurriría si fracasaran dichas defensas, pudiendo elaborar así hipótesis predictivas. Correlacionamos símbolos, ideales y latencia. Trabajamos respuestas adaptativas en latencia temprana y tardía. En la latencia temprana observamos respuestas regresivas más narcisistas propias de la oralidad primaria y secundaria que al fracasar dan lugar a respuestas fálico uretrales y genitales.Se observó una combinatoria de defensas más regresivas como la desmentida, acompañada de una represión secundaria. En la latencia tardía aparecen respuestas en las que prevalece el afecto ligado a escenas de cuidado (oral secundaria) en lugares activos, identificándose con roles materno y paternos. La defensa es operativa y el sadismo propio de la etapa puberal aveniente es desmentido y redireccionado; se trata de pulsiones sublimadas. La fantasía subyacente en las respuestas negativas está ligada a sensaciones corporales displacenteras, por la cercanía de la pubertad. Esas sensaciones son de desconocimiento del propio cuerpo y fundamentalmente aparecen repuestas relacionadas a los diques anímicos ligados al asco, la moral y la vergüenza. En la muestra total podremos observar mayores resultados y también trabajar más la diferencia por género.The “Desiderative Questionnaire” explores the development of child psyche, capturing the subtleties of the changing processes in different evolutionary moments. Study, from the symbols and arguments desires, fantasies, fears, mechanisms, symbolic capacity, capacity of processing losses and degree of cohesion achieved by the Self. We will research the typical answers in latent children, age that is crossed by lots of issues linked to development of the Oedipus complex and castration, as well as the closeness of the advent of puberty. On the other hand we understand the subjective process is continuous and allow to explore values, ideals, experiences that will realize certain personality traits or character in formation. This search it is a regulatory approach to the DQ in their children’s version in latent children (6 to 10) of CABA (Ciudad Autónoma de BuenosAires, Arg.). We have checked possible slogans by age, marking the need to ask for what they desire and not about what they like. Positive answers unfolds defensive repertoire from the adaptive at most regressive in sequential way. In negative answers appears what would happen if the defences fail, allowing to make predictive hypothesis. We correlate symbols, ideals and latency. We work the positive answers in early and late latency. In early latency we found regressive and narcissistic answers that belong to primary and secondary orality, to give place to phallic urethral and genitals answers when it fails. It was found combinatorial defences more regressive, like disproved follow by a secondary repression. In late latency appears answers where affection prevails tied to caring (secondary orality). The defence is useful and the own sadism that belongs to puberty it is disproved and redirect being sublimated drive. The underlying fantasy at the negative answers it is joined to unpleasureable body sensations because of the closeness to puberty. Those sensations are the ignorance about their own body, and generally appears answers relate to psychic dams like disgust, moral and shame. In the total sample we could watch more results and also work the difference by gender