Fire Activity Inside and Outside Protected Areas in Sub-Saharan Africa: A Continental Analysis of Fire and its Implications for Biodiversity and Land Management

Fire is an important ecological factor in many natural ecosystems. Without doubt one of the biomes with the highest fire activity in the world is the African savannah. Savannahs have evolved with fires since climate in these regions is characterized by definite dry and wet seasons that create the conditions for burning. During the wet months the herbaceous vegetation shows a quick growth, followed by a long dry period during which the abundant build-up of fine materials becomes highly flammable and most of fires occur. Animals and plants are adapted to these conditions and their lives depend on recurrent fires. In this context fire becomes an essential element to promote biodiversity and nature conservation. Park managers are using programmed fires as a tool to maintain the habitats and favorable conditions to the animal communities. Satellite products like burned areas and active fire maps are a valuable mean to analyze the fire activity and provide support to experts working for conservation and natural resource management. In the framework of the Digital Observatory for Protected Areas (DOPA), the MONDE group (Monitoring Natural Resources for Development) of the Joint Research Centre of the European Commission is using satellite products to analyze the fire occurrence and its effects on protected areas located in sub-Saharan Africa. Information on the fire activity was derived from the MODIS fire products (active fires and burned areas) and allows the DOPA to provide support to park managers as well as to experts working for conservation and natural resource management. We assessed 741 protected areas classified by the IUCN (International Union for Conservation of Nature) with a level of protection between class I and IV. The MODIS datasets are available since the year 2000 and were used to characterize the spatio-temporal distribution of fires over a period of 10 years. Information on fire activity was extracted for the protected areas and a 25km buffer zone around each of them. The region outside the protected areas was used for comparison in order to identify differences or similarities between their fire activities. This also contributed to understand how management and conservation influence fire and assess the level of isolation of the protected areas. The long time series allowed the identification of trends and the interannual variability in the fire activity. The dry season length was determined using FEWS RFE rainfall data (implemented at NOAA's Climate Prediction Center). Within each dry season we identified three periods (early, middle and late) in order to characterize the climatic and environmental conditions at which fires occur and identify trends and patterns. Every period of the dry season lasts two months and shows different conditions of temperature and drought level. Fire activity was characterized combining the information on active fires and burned areas. For each year we determined the fire seasonality, the fire frequency, the main vegetation types affected, the extent and intensity of burning. This information was also used to distinguish management fires from those related to other human activities like transhumance, agriculture and poaching in order to identify possible sources of threat to the protected areas. Information on the road network, the location of villages and cultivated fields were also included. Future work will include a combined analysis of fire activity and land-cover, land-cover change information so that management plans adopted in protected areas can be evaluated in their effectiveness to promote biodiversity and nature conservation.JRC.DDG.H.3-Global environement monitorin

Formation of a Unique Cluster of G-Quadruplex Structures in the HIV-1 nef Coding Region: Implications for Antiviral Activity

G-quadruplexes are tetraplex structures of nucleic acids that can form in G-rich sequences. Their presence and functional role have been established in telomeres, oncogene promoters and coding regions of the human chromosome. In particular, they have been proposed to be directly involved in gene regulation at the level of transcription. Because the HIV-1 Nef protein is a fundamental factor for efficient viral replication, infectivity and pathogenesis in vitro and in vivo, we investigated G-quadruplex formation in the HIV-1 nef gene to assess the potential for viral inhibition through G-quadruplex stabilization. A comprehensive computational analysis of the nef coding region of available strains showed the presence of three conserved sequences that were uniquely clustered. Biophysical testing proved that G-quadruplex conformations were efficiently stabilized or induced by G-quadruplex ligands in all three sequences. Upon incubation with a G-quadruplex ligand, Nef expression was reduced in a reporter gene assay and Nef-dependent enhancement of HIV-1 infectivity was significantly repressed in an antiviral assay. These data constitute the first evidence of the possibility to regulate HIV-1 gene expression and infectivity through G-quadruplex targeting and therefore open a new avenue for viral treatment. © 2013 Perrone et al

Photodynamic therapy and adapalene in dermatology: synergistic effects in immortal human keratinocytes

We compared the effects of methyl levulinate (MAL)/photodynamic therapy (PDT) and adapalene, evaluated singly, versus combination therapy on HaCaT keratinocytes. Our aim was to determine whether the additive/synergistic outcomes of combination treatment were such that doses of each component could be reduced without affecting treatment efficacy. The analysis of data from specific PDT/adapalene combinations results indicating effects ranging from additive to clearly synergistic. We first evaluated the effects on cell viability, cell cycle and protein expression profiles of each individual treatment, then we tried to understand the reasons and the mechanisms whereby cells under combined treatment move more efficiently to death. Although we observed therapeutic strengthening by increasing either drug doses or light fluences, reinforcement was particularly marked in combinations in which PDT was predominant. Thus, if PDT is appropriately tuned, the dose of the drug can be reduced without compromising the therapeutic response. As both photodynamic therapy and adapalene have been and are therapeutic approaches to treat different dermatological pathologies such as acne, actinic keratosis etc., our data suggest that the adapalene-PDT combination may have important spin-off in clinical dermatology as a strategy to tackle this nasty condition

Separase prevents genomic instability by controlling replication fork speed

Proper chromosome segregation is crucial for preserving genomic integrity, and errors in this process cause chromosome mis-segregation, which may contribute to cancer development. Sister chromatid separation is triggered by Separase, an evolutionary conserved protease that cleaves the cohesin complex, allowing the dissolution of sister chromatid cohesion. Here we provide evidence that Separase participates in genomic stability maintenance by controlling replication fork speed. We found that Separase interacted with the replication licensing factors MCM2-7, and genome-wide data showed that Separase co-localized with MCM complex and cohesin. Unexpectedly, the depletion of Separase increased the fork velocity about 1.5-fold and caused a strong acetylation of cohesin's SMC3 subunit and altered checkpoint response. Notably, Separase silencing triggered genomic instability in both HeLa and human primary fibroblast cells. Our results show a novel mechanism for fork progression mediated by Separase and thus the basis for genomic instability associated with tumorigenesis

Separase prevents genomic instability by controlling replication fork speed

Proper chromosome segregation is crucial for preserving genomic integrity, and errors in this process cause chromosome mis-segregation, which may contribute to cancer development. Sister chromatid separation is triggered by Separase, an evolutionary conserved protease that cleaves the cohesin complex, allowing the dissolution of sister chromatid cohesion. Here we provide evidence that Separase participates in genomic stability maintenance by controlling replication fork speed. We found that Separase interacted with the replication licensing factors MCM2-7, and genome-wide data showed that Separase co-localized with MCM complex and cohesin. Unexpectedly, the depletion of Separase increased the fork velocity about 1.5-fold and caused a strong acetylation of cohesin's SMC3 subunit and altered checkpoint response. Notably, Separase silencing triggered genomic instability in both HeLa and human primary fibroblast cells. Our results show a novel mechanism for fork progression mediated by Separase and thus the basis for genomic instability associated with tumorigenesis

Adenomyotic Cyst in a 25-Year-Old Woman: Case Report

Adenomyotic cysts are uncommon findings, usually in the context of diffuse adenomyosis and <5 mm in diameter. Herein we report a 4.5-cm adenomyotic cyst in a 25-year-old nulliparous woman with severe dysmenorrhea and pelvic pain. Transvaginal ultrasonography and magnetic resonance imaging revealed a well-circumscribed hypoechogenic mass in the posterior uterine wall, well separated from the uterine cavity. Pathologic analysis demonstrated that the cyst was lined with endometrial epithelium and stroma and was surrounded by smooth muscle hyperplasia. In the literature, we found 30 reports of cysts with similar characteristics. Because this cyst has not been clearly defined, it has been called by various names including adenomyotic cyst, cystic adenomyosis, and cystic adenomyoma. We believe this lesion should not be called an adenomyoma, but is more correctly called an adenomyotic cyst or, depending on age at onset, a juvenile adenomyotic cyst

Building capacity in remote sensing for conservation: present and future challenges

Remote sensing (RS) has made significant contributions to conservation and ecology; however, direct use of RS-based information for conservation decision making is currently very limited. In this paper, we discuss the reasons and challenges associated with using RS technology by conservationists and suggest how training in RS for conservationists can be improved. We present the results from a survey organized by the Conservation Remote Sensing Network to understand the RS expertise and training needs of various categories of professionals involved in conservation research and implementation. The results of the survey highlight the main gaps and priorities in the current RS data and technology among conservation practitioners from academia, institutions, NGOs and industry. We suggest training to be focused around conservation questions that can be addressed using RS-derived information rather than training pure RS methods which are beyond the interest of conservation practitioners. We highlight the importance of developing essential biodiversity variables (EBVs) and how this can be achieved by increasing the RS capacity of the conservation community. Moreover, we suggest that open-source software is adopted more widely in the training modules to facilitate access to RS data and products in developing countries, and that online platforms providing mapping tools should also be more widely distributed. We believe that improved RS capacity among conservation scientists will be essential to improve conservation efforts on the ground and will make the conservation community a key player in the definition of future RS-based products that serve conservation and ecological needs

Burnout in cardiac anesthesiologists. results from a national survey in italy

Objective: There is increasing burnout incidence among medical disciplines, and physicians working in emergency settings seem at higher risk. Cardiac anesthesiology is a stressful anesthesiology subspecialty dealing with high-risk patients. The authors hypothesized a high risk of burnout in cardiac anesthesiologists. Design: National survey conducted on burnout Setting: Italian cardiac centers. Participants: Cardiac anesthesiologists. Interventions: The authors administered via email an anonymous questionnaire divided into 3 parts. The first 2 parts evaluated workload and private life. The third part consisted of the Maslach Burnout Inventory test with its 3 constituents: high emotional exhaustion, high depersonalization, and low personal accomplishment. Measurements and Main Results: The authors measured the prevalence and risk of burnout through the Maslach Burnout Inventory questionnaire and analyzed factors influencing burnout. Among 670 contacts from 71 centers, 382 cardiac anesthesiologists completed the survey (57%). The authors found the following mean Maslach Burnout Inventory values: 14.5 ± 9.7 (emotional exhaustion), 9.1 ± 7.1 (depersonalization), and 33.7 ± 8.9 (personal accomplishment). A rate of 34%, 54%, and 66% of respondents scored in “high” or “moderate-high” risk of burnout (emotional exhaustion, depersonalization, and personal accomplishment, respectively). The authors found that, if offered to change subspecialty, 76% of respondents would prefer to remain in cardiac anesthesiology. This preference and parenthood were the only 2 investigated factors with a protective effect against all components of burnout. Significantly lower burnout scores were found in more experienced anesthesiologists. Conclusion: A relatively high incidence of burnout was found in cardiac anesthesiologists, especially regarding high depersonalization and low personal accomplishment. Nonetheless, most of the respondents would choose to remain in cardiac anesthesiology

ACP Fire Monitoring Tool for protected area management

This report documents the context and the objectives of a new Fire Monitoring Tool developed in the framework of the JRC activity in African, Caribbean and Pacific (ACP) Countries. Vegetation fires are widespread in Africa and are a key component of many ecosystems. They can be a cause of threat and damage for ecosystems not adapted to them, but for many African habi-tats fires are just as important as rain. As a consequence of this, dedicated fire programs are often included in the protected areas conservation plans to maintain or improve the ecosystem structure and the landscape diversity that are essential to biodiversity. Information on fire activity becomes therefore critical for the implementation of conservation programs and the man-agement of protected areas. With the support of Earth Observation (EO), fire monitoring is possible, at global level, over long time periods. However, EO products require technological know-how which is often limited for many ecologists and park managers. In this study we present a new tool for fire monitoring that we developed especially for people working in conservation and park management. The tool is a web client that provides historical and near-real time indicators of the fire activity derived from the Moderate Resolution Imaging Spectroradiometer (MODIS) burned area and active fire products. The MODIS products are dis-tributed by NASA-FIRMS and the University of Maryland and cover more than a decade going from late 2000 to present. The web client is available on-line and produces, on the fly, graphs, tables and maps of the fire activity, for a selected protected area and period of time. The content and its graphical represen-tation have been designed to provide a synthesis of the fire activity with no need of additional softwares to visualize or use the data.JRC.H.5-Land Resources Managemen

On the Contribution of Remote Sensing to DOPA, a Digital Observatory for Protected Areas

The Digital Observatory for Protected Areas (DOPA) is a biodiversity information system currently developed as a set of interoperable web services at the Joint Research Centre of the European Commission in collaboration with other international organizations, including GBIF, UNEP-WCMC, Birdlife International and RSPB. DOPA is not only designed to assess the state and pressure of Protected Areas (PAs) and to prioritize them accordingly, in order to support decision making and fund allocation processes, but it is also conceived as a monitoring and modeling service. To capture the dynamics of spatio-temporal changes in habitats and anthropogenic pressure on PAs, the automatic collection and processing of remote sensing data are processes at the heart of the system. In particular, DOPA uses information from EumetCAST and SpotVGT to compute environmental trends and detect anomalies every 10 days. Anthropogenic threats are also currently assessed through the analysis of agricultural pressure, population growth and habitat fragmentation around the protected areas. Fire activity in sub-Saharan protected areas which is derived from the MODIS fire products (active fires and burned areas) provide further support to park managers as well as to experts working for conservation and natural resource management. The purpose of this paper is to highlight the variety of uses of remote sensing data by the DOPA, the integration with other data sources, the practical implementation according to an architecture grounded in international initiatives such as GEOSS, GSDI and INSPIRE, and applications in monitoring and in ecological forecasting through e-Habitat, DOPAs¿ habitat modeling service.JRC.H.3-Global environement monitorin