Broad Line Radio Galaxies: Jet Contribution to the nuclear X-Ray Continuum

It is shown that, for Broad Line Radio Galaxies the strength of the non-thermal beamed radiation, when present, is always smaller than the accretion flow by a factor < 0.7 in the 2-10 keV band. The result has been obtained using the procedure adopted for disentangling the Flat Spectrum Radio Quasar 3C 273 (Grandi & Palumbo 2004). Although this implies a significantly smaller non-thermal flux in Radio Galaxies when compared to Blazars, the jet component, if present, could be important at very high energies and thus easily detectable with GLAST.Comment: 12 pages including 2 figures (4 files), ApJ accepte

Extremely Luminous Far-infrared Sources (ELFS)

The Infrared Astronomical Satellite (IRAS) survey uncovered a class of Extremely Luminous Far Infrared Sources (ELFS), exhibiting luminosities up to and occasionally exceeding 10 to the 12th power L sub 0. Arguments are presented to show that sources with luminosities L equal to or greater than 3 x 10 to the 10th power L sub 0 may represent gas rich galaxies in collision. The more conventional explanation of these sources as sites of extremely active star formation fails to explain the observed low optical luminosities of ELFS as well as their high infrared excess. In contrast, a collisional model heats gas to a temperature of approx. 10 to the 6th power K where cooling takes place in the extreme ultraviolet. The UV is absorbed by dust and converted into far infrared radiation (FIR) without generation of appreciable optical luminosity. Gas recombination as it cools generates a Lyman alpha photon only once for every two extreme ultraviolet approx. 50eV photons emitted by the 10 to the 6th power gas. That accounts for the high infrared excess. Finally, the model also is able to explain the observed luminosity distribution of ELFS as well as many other traits

Formation of a Unique Cluster of G-Quadruplex Structures in the HIV-1 nef Coding Region: Implications for Antiviral Activity

G-quadruplexes are tetraplex structures of nucleic acids that can form in G-rich sequences. Their presence and functional role have been established in telomeres, oncogene promoters and coding regions of the human chromosome. In particular, they have been proposed to be directly involved in gene regulation at the level of transcription. Because the HIV-1 Nef protein is a fundamental factor for efficient viral replication, infectivity and pathogenesis in vitro and in vivo, we investigated G-quadruplex formation in the HIV-1 nef gene to assess the potential for viral inhibition through G-quadruplex stabilization. A comprehensive computational analysis of the nef coding region of available strains showed the presence of three conserved sequences that were uniquely clustered. Biophysical testing proved that G-quadruplex conformations were efficiently stabilized or induced by G-quadruplex ligands in all three sequences. Upon incubation with a G-quadruplex ligand, Nef expression was reduced in a reporter gene assay and Nef-dependent enhancement of HIV-1 infectivity was significantly repressed in an antiviral assay. These data constitute the first evidence of the possibility to regulate HIV-1 gene expression and infectivity through G-quadruplex targeting and therefore open a new avenue for viral treatment. © 2013 Perrone et al

Variable X-ray absorption in the mini-BAL QSO PG 1126-041

X-ray studies of active galactic nuclei (AGN) with powerful nuclear winds are important for constraining the physics of the inner accretion/ejection flow around supermassive black holes (SMBHs) and for understanding the impact of such winds on the AGN environment. Our main scientific goal is to constrain the properties of the circum-nuclear matter close to the SMBH in the mini-broad absorption line quasar (mini-BAL QSO) PG 1126-041 using a multi-epoch observational campaign with XMM-Newton. We performed temporally resolved X-ray spectroscopy and simultaneous UV and X-ray photometry on the most complete set of observations and on the deepest X-ray exposure of a mini-BAL QSO ever. We found complex X-ray spectral variability on time scales of both months and hours, which is best reproduced by means of variable massive ionized absorbers along the line of sight. As a consequence, the observed optical-to-X-ray spectral index is found to be variable with time. In the highest signal-to-noise observation we detected highly ionized X-ray absorbing material outflowing much faster (v ~ 16500 km/s) than the UV absorbing one (v ~ 5000 km/s). This highly ionized absorber is found to be variable on very short (a few kiloseconds) time scales. Our findings are qualitatively consistent with line-driven accretion disk winds scenarios. Our observations have opened the time-resolved X-ray spectral analysis field for mini-BAL QSOs. Only with future deep studies will we be able to map the dynamics of the inner flow and understand the physics of AGN winds and their impact on the environment.Comment: Replaced to match the published versio

High-Energy Spectra of Active Galactic Nuclei. II. Absorption in Seyfert Galaxies

Absorption by cold material in a large sample of active galaxies has been analyzed in order to study statistically the behavior of absorbed sources. The analysis indicates that on the basis of the column density alone, sources can be divided into low-absorption ([NH/NHGal] ? 50) and high-absorption ([NH/NHGal] ? 50) objects. While the second group consists mostly of narrow emission line galaxies (Seyfert galaxies of type 1.9-2), the first group is less homogenous, being formed by a mixture of broad and narrow emission line objects (Seyfert 1-2 galaxies). A study of the distribution of the column density values by means of bootstrap analysis confirms the reality of this effect. One group consisting of optically selected objects is well explained within the unified theory as nuclei obscured by a molecular torus. The second group made up of X-ray- and IRAS-selected objects is more difficult to define: in these sources the absorption is underestimated owing to difficulties (1) in fitting complex absorption spectra or (2) in measuring NH values in Compton-thick sources or the absorption has a different origin than in the torus. Possible correlations of absorption with X-ray luminosity, axial ratio, and Balmer decrement have also been investigated. Previous suggestions that lower luminosity AGNs tend to be more highly absorbed than those with higher luminosity are not confirmed by the present data; neither is any evidence for a correlation of NH with axial ratio (b/a) found except for a preference of Seyfert 1-1.5 galaxies to be in face-on galaxies. While some sources (Seyfert 1-1.5 galaxies and low-absorption objects) have X-ray absorption compatible with Balmer decrement, high-absorption objects have column densities much higher than predicted from optical observations. These results are in agreement with the unified theory since the torus parameters are expected to be independent of luminosity, its orientation should be random with respect to the host galaxy, and its location should be in between the broad- and narrow-line regions. A study of the NH variability indicates that in a large fraction (70%) of the sources for which the analysis could be done, NH varies on timescales from months to years. In Seyfert 1-1.5 galaxies, the variability is associated with a region in or near the broad-line region and is explained in terms of partial covering and/or warm absorption models. In Seyfert 2 galaxies, the only variability observed is that associated with narrow emission line galaxies. The study of the column density distributions indicates that Seyfert 1-1.5 galaxies are characterized by NH = 18+9?7 ? 1021 atoms cm-2. Seyfert 1.9-2 galaxies have instead NH = 96+54?35 ? 1021 atoms cm-2 and a larger dispersion; if this group is divided into low- and high-absorption objects, NH = 14.5+7.2?5.3 ? 1021 atoms cm-2 and NH = 132.8+80.1?52.6 ? 1021 atoms cm-2, respectively, are obtained. The observed dispersion in each group is consistent with being entirely due to column density variability

Radio Lobes of Pictor A: an X-ray spatially resolved Study

A new XMM observation has made possible a detailed study of both lobes of the radio galaxy Pictor A. Their X-ray emission is of non thermal origin and due to Inverse Compton scattering of the microwave background photons by relativistic electrons in the lobes, as previously found. In both lobes, the equipartition magnetic field (Beq) is bigger than the Inverse Compton value (Bic), calculated from the radio and X-ray flux ratio. The Beq/Bic ratio never gets below 2, in spite of the large number of reasonable assumptions tested to calculate Beq, suggesting a lobe energetic dominated by particles. The X-ray data quality is good enough to allow a spatially resolved analysis. Our study shows that Bic varies through the lobes. It appears to increase behind the hot spots. On the contrary, a rather uniform distribution of the particles is observed. As a consequence, the radio flux density variation along the lobes appears to be mainly driven by magnetic field changes.Comment: 15 pages, 3 figures, ApJ accepte

Polyhydroxyalkanoates production by an advanced food-on-demand strategy: The effect of operational conditions

Despite the increasing number of studies related to the polyhydroxyalkanoate (PHA) production from sewage sludge of wastewater treatment plants, there is still a gap in the correlation between the operational conditions, such as the organic loading rate (OLR), and the intracellular polyhydroxyalkanoate (PHA) content, productivity and final recovery of the polymer. Therefore, this work aims to provide experimental data on PHA productivity and purity in view of scaling up the process to an industrial level taking into account process parameters. In view of that, three OLR were applied during the selection of PHA-accumulating bacteria in sewage sludge. Then, the biomass was harvested and subjected to batch accumulation experiments at two organic loads per dosage by employing a tailor-made software to adopt an automated feed-on-demand strategy, which allowed for 30–56 h of accumulation tests in stand-alone mode. Finally, an improved protocol for PHA extraction has been applied. Experimental results show that the maximum PHA content (60% w/w) was achieved using the highest organic load per dosage during the accumulation test with the biomass selected at the highest OLR (1.8 g COD L−1 d−1). Also, the extraction protocol efficiency was proven with four samples with different PHA content, achieving recovery yield as high as 78 ± 3 % with a purity of 89 ± 2 %, thus demonstrating that the adopted strategy might be beneficial for industrial use

BEAT: Bioinformatics Exon Array Tool to store, analyze and visualize Affymetrix GeneChip Human Exon Array data from disease experiments

<p>Abstract</p> <p>Background</p> <p>It is known from recent studies that more than 90% of human multi-exon genes are subject to Alternative Splicing (AS), a key molecular mechanism in which multiple transcripts may be generated from a single gene. It is widely recognized that a breakdown in AS mechanisms plays an important role in cellular differentiation and pathologies. Polymerase Chain Reactions, microarrays and sequencing technologies have been applied to the study of transcript diversity arising from alternative expression. Last generation Affymetrix GeneChip Human Exon 1.0 ST Arrays offer a more detailed view of the gene expression profile providing information on the AS patterns. The exon array technology, with more than five million data points, can detect approximately one million exons, and it allows performing analyses at both gene and exon level. In this paper we describe BEAT, an integrated user-friendly bioinformatics framework to store, analyze and visualize exon arrays datasets. It combines a data warehouse approach with some rigorous statistical methods for assessing the AS of genes involved in diseases. Meta statistics are proposed as a novel approach to explore the analysis results. BEAT is available at <url>http://beat.ba.itb.cnr.it</url>.</p> <p>Results</p> <p>BEAT is a web tool which allows uploading and analyzing exon array datasets using standard statistical methods and an easy-to-use graphical web front-end. BEAT has been tested on a dataset with 173 samples and tuned using new datasets of exon array experiments from 28 colorectal cancer and 26 renal cell cancer samples produced at the Medical Genetics Unit of IRCCS Casa Sollievo della Sofferenza.</p> <p>To highlight all possible AS events, alternative names, accession Ids, Gene Ontology terms and biochemical pathways annotations are integrated with exon and gene level expression plots. The user can customize the results choosing custom thresholds for the statistical parameters and exploiting the available clinical data of the samples for a multivariate AS analysis.</p> <p>Conclusions</p> <p>Despite exon array chips being widely used for transcriptomics studies, there is a lack of analysis tools offering advanced statistical features and requiring no programming knowledge. BEAT provides a user-friendly platform for a comprehensive study of AS events in human diseases, displaying the analysis results with easily interpretable and interactive tables and graphics.</p