La colaboración empresa-universidad en el ámbito de las razas ovinas autóctonas españolas: el ejemplo de Melovine

Se describe la colaboración establecida desde los años 90 hasta la actualidad entre un laboratorio veterinario (CEVA Salud Animal) y una universidad española (Universidad de Zaragoza), con la hormona pineal melatonina como eje. Los objetivos fundamentales han sido la descripción de los mecanismos básicos de actuación de esta hormona sobre la estacionalidad reproductiva de la especie ovina y la realización de pruebas de campo en ganaderías comerciales españolas, en un buen número de razas autóctonas, con el fin de difundir los resultados obtenidos en condiciones reales de explotación. Este hecho ha involucrado a todos los eslabones del sector ovino español, desde el laboratorio, el ganadero, la cooperativa, el veterinario, las asociaciones de defensa sanitaria, las administraciones públicas, las asociaciones de razas, coordinados todos ellos por un departamento universitario. Se ha trabajado con las razas autóctonas Castellana, Churra, Latxa, Manchega, Merina, Ojalada, Ojinegra, Rasa Aragonesa, Ripollesa y Segureña, además de otras como la Assaf y Lacaune. Se han descrito protocolos de uso de los implantes de melatonina en ovejas adultas, corderas y moruecos, en distintos momentos fisiológicos y sistemas de explotación. Todos ellos han sido difundidos al sector ovino español a través de publicaciones científicas y técnicas y a través de congresos, ponencias y cursos destinados a veterinarios y ganaderos

2D experiments and numerical simulation of the oscillatory shallow flow in an open channel lateral cavity

Steady shallow flows past an open channel lateral cavity can induce the excitation of an eigenmode of a gravity standing wave inside the cavity, called seiche, which may be coupled with the shedding of vortices at the opening of the cavity. The presence of the seiche is of fundamental interest as it enhances the mass exchange between the main channel and the cavity. Measurements of the time evolution of the water surface are not often found in the literature for this type of flows. In this work, an experimental and numerical study of a shallow flow past a channel lateral cavity is carried out. The main novelty is the use of a pioneering non-intrusive experimental technique to measure the water surface at the channel-cavity region. This optical technique offers high resolution 2D data in time and space of the water surface evolution, allowing to determine the relevant features of the seiche oscillation. Such data are supplemented with Particle Image Velocimetry measurements. Furthermore, the experiments are numerically reproduced using a high-resolution depth-averaged URANS shallow water model, under the assumption that shallow water turbulence is mainly horizontal. The experimental and numerical results are analyzed in the frequency domain. High-resolution two-dimensional amplitude oscillation maps of the seiche phenomenon, as well as velocity fields, are presented. The high quality of the experimental data reported in this work makes this data set a suitable benchmark for numerical simulation models in order to evaluate their performance in the resolution of turbulent resonant shallow flows

Short communication. In vitro embryo production can be modified by the previous ovarian response to a superovulatory treatment in sheep

Thirty-two ewes were used to study how the ovarian response to a superovulatory treatment determines quality of oocytes recovered from ovaries after embryo collection, and their developmental capacity after in vitro maturation (IVM) and fertilization (IVF). Ewes were superovulated, and seven days after oestrus, embryos were collected and ewes divided into three groups: (+ +), n=19, ewes responding to the treatment with embryos collected after flushing; (+ –), n=8, ewes responding, but only oocytes were found; and (– –), n=5, ewes not responding to the treatment and no embryos collected. Ovaries were recovered and oocytes collected from the three groups. A significant effect of the response to the treatment was observed for oocyte quality, so that (– –) ewes presented the higher number of oocytes per ewe (p<0.001). Total number of oocytes selected for IVM and IVF was significantly higher in the same group, in comparison with (+ +) and (+ –) (p<0.001). Group (+ –) ewes presented the lowest maturation (p<0.001), fertilization (p<0.05) and cleavage rates (p<0.001). In conclusion, the ovarian response to a superovulatory treatment determines the number and quality of the oocytes recovered 7 days after the oestrus induced by the hormonal treatment. In vitro techniques could be an important tool to increase embryo production by particular ewes when they are not able to produce a significant amount of in vivo embryos

¿Pueden los factores clínicopatológicos mejorar la predicción de metástasis en ganglios linfáticos no centinelas en pacientes con cáncer de mama?

Introduction: To determine whether clinicopathological characteristics can improve the prediction of metastasis to nonsentinel lymph nodes (NSLNs) over the use of only mRNA copy number in sentinel lymph node (SLN) biopsies. Methods: This was a retrospective, observational study that included a total of 824 patients with T1-3 breast cancer who had clinically negative, ultrasound-negative axilla without evidence of metastasis and who underwent one-step nucleic acid amplification in SLN biop-sies. Results: 118 required a complete axillary lymph node dissection (ALNhD). About 35.6% (42/118) had metastases to a NSLN, and 64.4% (76/118) had no metastasis to a NSLN. The ROC curve of the total tumor load (TTL) presented an area under the curve (AUC) of 0.651 (95%; CI: 0.552-0.751). The 7294 copies of CK19 mRNA were established as the optimal cutoff point, with sensitivity: 93%, specificity: 63%, positive predictive value: 44%, and negative predictive value: 91%. By as-sociating the clinicopathological parameters (multicentricity, pooled immunohistochemistry [IHC], and progesterone receptors), the AUC went up to 0.752 (95% CI: 0.663-0.841). Conclusions: Clinicopathological factors should be considered together with the total CK19 mRNA copy number (the TTL) of the SLNs to improve the predictive capacity of metastatic involvement of the NSLNs

A systematic approach for performance assessment using process mining. An industrial experience report

Software performance engineering is a mature field that offers methods to assess system performance. Process mining is a promising research field applied to gain insight on system processes. The interplay of these two fields opens promising applications in the industry. In this work, we report our experience applying a methodology, based on process mining techniques, for the performance assessment of a commercial data-intensive software application. The methodology has successfully assessed the scalability of future versions of this system. Moreover, it has identified bottlenecks components and replication needs for fulfilling business rules. The system, an integrated port operations management system, has been developed by Prodevelop, a medium-sized software enterprise with high expertise in geospatial technologies. The performance assessment has been carried out by a team composed by practitioners and researchers. Finally, the paper offers a deep discussion on the lessons learned during the experience, that will be useful for practitioners to adopt the methodology and for researcher to find new routes

Efecto de los implantes de melatonina y la presencia de compan~eros sobre los rendimientos de los moruecos durante la monta

Para evaluar el efecto de los implantes de melatonina sobre la capacidad de monta del morueco en pri- mavera, tanto en cubriciones en grupo como individuales, a inicios de febrero 6 machos recibieron tres implantes (M), mientras que otros 6 permanecieron como control (C). A finales de marzo 60 ovejas sin- cronizadas en celo se dividieron en 3 apartados (n = 20), introducie´ndose 4 machos en cada grupo (2M+2C). Durante 6 horas se realizo´ un test de monta, registra´ndose el nu´mero de montas y servicios y el intervalo entre servicios. Cuatro di´as ma´s tarde, 60 ovejas sincronizadas se dividieron en 12 apar- tados (n = 5), introduciendo un macho en cada grupo para realizar un test individual. Los machos tra- tados presentaron una mayor actividad sexual que los control (C: 24,5 ± 3,0; M: 25,9 ± 2,3 montas+ser- vicios; P < 0,05) y un menor intervalo entre servicios (C: 36,6 ± 6,6; M: 27,6 ± 2,1 min; P < 0,05). En grupo los machos fueron capaces de montar y servir ma´s veces que de forma individual (Grupo: 27,8 ± 3,1; In- dividual: 22,6 ± 1,8; P < 0,05). En conclusio´n, el tratamiento de los moruecos con implantes de melato- nina en primavera dio lugar a un aumento del nu´mero y la frecuencia de montas y servicios. Adema´s, se observo´ una mayor actividad sexual cuando los machos se encontraban en grupo que en solitario, independientemente del tratamiento

BT595, a 10% Human Normal Immunoglobulin, for Replacement Therapy of Primary Immunodeficiency Disease: Results of a Subcohort Analysis in Children

Children; Pharmacokinetics; Serious bacterial infectionsNiños; Farmacocinética; Infecciones bacterianas gravesNens; Farmacocinètica; Infeccions bacterianes greusPurpose To assess the efficacy, pharmacokinetics, and safety of a new, highly purified 10% IVIg (BT595, Yimmugo®) administered in children with PID. Methods This was an open-label, prospective, uncontrolled, multicenter Phase III pivotal trial. Among the 67 subjects in the trial were 18 pediatric patients aged 2 to 17 years with diagnosis of PID included in this analysis. They received doses between 0.2 and 0.8 g/kg body weight for approximately 12 months at intervals of either 3 or 4 weeks. Dosage and dosing interval were based on each patient’s pre-trial infusion schedule. The rates of acute serious bacterial infections (SBI), secondary efficacy, safety, and pharmacokinetic outcomes were evaluated. Results No SBI occurred in the pediatric population. Two hundred sixty infusions were administered to the 18 pediatric patients. The mean (SD) IgG trough level was 8.55 (1.67) g/L at baseline and 8.84 (2.17) g/L at the follow-up visit after the last BT595 infusion. At the single infusions respectively, the average mean IgG trough levels ranged between 8.52 and 10.58 g/L. More than 85% of all infusions administered were not associated with any infusional AE (start during or within 72 h post-infusion). None of the severe or serious AEs were related to the investigational medicinal product (IMP). No premedication was used. Thirteen children reached a maximum infusion rate between > 2.0 and 8 mL/kg/h; no AE with an onset during the infusion occurred at these infusion rates. Conclusion BT595 is effective, convenient, well tolerated, and safe for the treatment of children with PID.This trial was funded by Biotest AG, Dreieich, Germany