DISEÑO DE UN SOFTWARE R-MICMAC COMO COMPLEMENTO EN EL ANÁLISIS PARA EL CONSENSO DE LOS EXPERTOS EN LA INVESTIGACIÓN DESIGN OF AN R-MICMAC SOFTWARE AS A COMPLEMENT IN THE ANALYSIS FOR THE CONSENSUS OF THE EXPERTS IN THE INVESTIGATION

El objetivo de la investigación fue diseñar un software R-MICMAC como complemento en el análisis para el consenso de los expertos en la investigación. Se basó en un estudio tecnológico con apoyo en una revisión documental. Para el desarrollo del programa se empleó el software R de libre acceso. Adicionalmente, se usó el software Restudio, en un ambiente que permitió interactuar con el software R y generar acceso al comando de ayuda. El diseño del software R-MICMAC resuelve los inconvenientes presentados por el MICMAC, al obtener la misma matriz de salida que ofrece MICMAC y al generar el listado de programa y algoritmo es posible cualquier modificación que convenga al usuario. Además, se pueden introducir tantas matrices como expertos se hayan consultado y el programa determina la matriz de consenso para ejecutar el algoritmo del MICMAC. Asimismo, posibilita que las tablas resultantes del procesamiento de la información se exporten directamente a Excel, desde donde es posible desarrollar otros cálculos y gráficos con mayor facilidad. El software diseñado requiere del proceso de validación teórica y práctica, que permita accionar en la praxis investigativa

The outcome-representation learning model: impairments in decision-making in adolescents with excess weight

Impairments in decision-making have been suggested as a predisposing factor to obesity development. Individuals with excess weight display riskier decisions than normal weight people. Furthermore, adolescence is a period of life in which risky behavior may increase. We aimed to investigate decision making applying the Outcome-Representation-Learning (ORL) model to the Iowa Gambling Task (IGT) in adolescents with excess weight. Twenty-nine excess weight and twenty-eight normal weight adolescents, classified according to their age-adjusted body mass index (BMI) percentile, participated in the study. Decision-making was measured using the IGT. A Bayesian computational ORL model was applied to assess reward learning, punishment learning, forgetfulness, win perseverance and deck perseverance. The IGT net score was lower in excess weight than normal weight adolescents (ß = 2.85; p <.027). Reward learning (95% HDI 0.011, 0.232]) was higher, while forgetfulness (95% HDI - 0.711, - 0.181]) and deck perseverance (95% HDI - 3.349, - 0.203]) were lower, in excess weight than normal weight adolescents. Excess weight adolescents seemed better at learning the most rewarding choices and showed a random strategy based on reward and novelty seeking. Consequently, excess weight adolescents made more disadvantageous selections, and performed worse in the IGT

Development of pathogenicity predictors specific for variants that do not comply with clinical guidelines for the use of computational evidence

Predictors de patogenicitat in silico; Variants missense; Seqüenciació de nova generacióPredictores de patogenicidad in silico; Variantes missense; Secuenciación de nueva generaciónIn silico pathogenicity predictors; Missense variants; Next-generation sequencingBackground Strict guidelines delimit the use of computational information in the clinical setting, due to the still moderate accuracy of in silico tools. These guidelines indicate that several tools should always be used and that full coincidence between them is required if we want to consider their results as supporting evidence in medical decision processes. Application of this simple rule certainly decreases the error rate of in silico pathogenicity assignments. However, when predictors disagree this rule results in the rejection of potentially valuable information for a number of variants. In this work, we focus on these variants of the protein sequence and develop specific predictors to help improve the success rate of their annotation. Results We have used a set of 59,442 protein sequence variants (15,723 pathological and 43,719 neutral) from 228 proteins to identify those cases for which pathogenicity predictors disagree. We have repeated this process for all the possible combinations of five known methods (SIFT, PolyPhen-2, PON-P2, CADD and MutationTaster2). For each resulting subset we have trained a specific pathogenicity predictor. We find that these specific predictors are able to discriminate between neutral and pathogenic variants, with a success rate different from random. They tend to outperform the constitutive methods but this trend decreases as the performance of the constitutive predictor improves (e.g. with PON-P2 and PolyPhen-2). We also find that specific methods outperform standard consensus methods (Condel and CAROL). Conclusion Focusing development efforts on the case of variants for which known methods disagree we may obtain pathogenicity predictors with improved performances. Although we have not yet reached the success rate that allows the use of this computational evidence in a clinical setting, the simplicity of the approach indicates that more advanced methods may reach this goal in a close future.This work has been supported by the spanish Ministerio de Economía y Competitividad (BIO2012–40133; SAF2016–80255-R). It has also been supported, and the publication costs have been defrayed, by the European Regional Development Fund (ERDF), through the Interreg V-A Spain-France-Andorra programme (POCTEFA 2014–2020), research grant PIREPRED (EFA086/15)

Análisis de la incidencia de la educación financiera y la competencia digital en la intención de emprendimiento de la población económicamente activa en la ciudad de Cuenca 2022

El emprendimiento, como un acto de innovación con la finalidad de producir riquezas, surge de la necesidad de empleo y la escasez de ingresos. Por ello una parte de la economía se ha dedicado a estudiar, sobre todo, tres constructos: la Intención de Emprendimiento (IE), la Educación Financiera (EF) y la Competencia Digital (CD). El objetivo de este trabajo es construir un modelo para determinar la relación de significancia entre estas variables en la zona urbana de la ciudad de Cuenca. El estudio es de tipo correlacional, transversal con enfoque cuantitativo, mediante el análisis factorial y el uso de ecuaciones estructurales a partir de datos levantados a través de encuestas. La muestra examinada consta de 386 encuestas estratificadas en 15 parroquias urbanas de la ciudad. En los resultados más destacados se encuentra la viabilidad del modelo, dado que el análisis incorpora a la CD como variable mediadora de la relación con un efecto indirecto del 0,821. Además, se agregan los resultados positivos y significativos entre la EF y la IE de 0,289. Se recomienda a los emprendedores potenciar su conocimiento accediendo a programas públicos y privados para incrementar las habilidades en estas áreas para dar lugar a la creación de nuevas unidades de negocioEntrepreneurship, as an act of innovation to produce wealth, arises from the need for employment and the scarcity of income. For this reason, a part of the economy has been dedicated to studying, above all, three conduits: Entrepreneurship Intention (IE), Financial Education (FE) and Digital Competence (DC). The purpose of this work is to build a model to determine the significance relationship between these variables in the urban area of the city of Cuenca. Th type of the study is correlational, cross-sectional with a quantitative approach, through factor analysis and the use of structural equations from data collected through surveys. The examined sample consists of 386 stratified surveys in 15 urban parishes of the city. Among the most outstanding results is the viability of the model, given that the analysis incorporates DC as a mediating variable of the relationship with an indirect effect of 0.821. Furthermore, the positive and significant results between FE and IE of 0.289 are added. It is recommended that entrepreneurs enhance their knowledge by accessing public and private programs to increase skills in these areas in order to give rise to the creation of business units.Ingeniero ComercialCuenc

Implementación de un sistema de gestión de inventarios para la localización de vehículos en los patios de almacenamiento de la Empresa CIAUTO Cía. Ltda.

El presente trabajo tuvo como objetivo implementar un Sistema de Gestión de Inventarios para la locación de vehículos en los patios de almacenamiento de la empresa CIAUTO CIA LTDA. Donde se desarrolló un método descriptivo debido a que de los valores de interés se fueron describiendo de manera significativa, donde se registraron las variables o se miden las mismas con la finalidad de definir las propiedades primordiales, en este caso a los automotores a almacenarse y se inició mediante la recolección de datos, mismo que fue de vital importancia para conocer la cantidad de automotores que se fabrican, y el error a permitirse debe ser el mínimo posible en caso de no poderse eliminar por completo, también se identificó el tipo de manejo de inventarios que se dispone, para a partir del mismo y de las necesidades de la empresa establecer una distribución de inventario de acuerdo a la metodología de calificación de inventarios ABC, definiendo que productos tienen una mayor demanda por parte de los clientes. Obteniendo como resultado que se han categorizado como clase A, B y C, mediante la distribución del mismo nombre y haberse determinado una proyección de la demanda para años posteriores que la demanda en el año 2022, para la marca Great Wall será de 4283 unidades, la marca DFSK de 2225 vehículos y la marca Shineray y Soueast tendrán un requerimiento de 2400 y 1872 automotores, dando un total de vehículos requeridos de 10780 en el año 2022. Concluyendo que la principal desventaja identificada dentro de la empresa CIAUTO CIA. LTDA es que no utilizan los patios de almacenamiento a su máxima capacidad. Se recomienda hacer uso adecuado de la aplicación web e instalar repetidores Wi-fi en los lugares más alejados de los patios de almacenamiento.The objective of this research was to implement an Inventory Management System for the location of vehicles in the storage yards of CIAUTO CIA LTDA, where a descriptive method was performed because the values of interest were described in a meaningful way, where the variables were registered or measured with the purpose of defining the primordial properties to the vehicles to be storaged and started with the data collection, which was essential to know the quantity of vehicles that are manufactured and the error to be allowed should be the minimum possible in case of not being able to be completely eliminated. It was also identified the type of inventory management that is available in order to establish an inventory distribution according to the ABC inventory qualification methodology, determining the products that have a higher demand from their customers. As a result, the products have been categorized as class A, B and C through the distribution of the same name and a demand projection has been determined for subsequent years. The demand in 2022 for the Great Wall brand will be 4283 units, the DFSK brand will have 2225 vehicles and the Shineray and Soueast brands will have a requirement of 2400 and 1872 vehicles, giving a total of 10,780 vehicles required in 2022. In conclusion, the main disadvantage identified within CIAUTO CIA. LTDA is that they do not use the storage yards to their maximum capacity and recommending making adequate use of the web application and install Wi-fi repeaters in the places farthest from the storage yards

Manejo del dolor infantil por los profesionales de enfermería

Introducción: La sensación de dolor es subjetiva y en el caso de la infancia se hace más compleja su valoración debido a las dificultades que los niños tienen para comunicarse. Por tanto, se tiende a infravalorar su dolor y el tratamiento es inadecuado. Este artículo está centrado en los profesionales de Enfermería que cuidan a diario a niños que están sometidos a situaciones dolorosas por estar ingresados o padecer alguna alteración de salud. El objetivo es evaluar el grado de conocimiento de los profesionales de Enfermería sobre el dolor infantil. Metodología: Se realizó un estudio cuasiexperimental de tipo pretest-postest, en el que se distinguieron 3 Fases: I: Medida del grado de conocimiento sobre el dolor pediátrico mediante un cuestionario ya validado. II: Intervención formativa para mejorar los conocimientos, la actitud y las habilidades necesarias para el manejo adecuado del dolor. III: Evaluación de la intervención y del nivel de conocimientos tras la misma. Resultados: El resultado global más importante es que el 57% de los profesionales poseen un nivel de conocimientos considerado “Alto” y el 30% posee un nivel “Medio”. Este nivel de conocimientos es mayor en las unidades de Neonatología e inferior en las unidades de Maternidad. Conclusiones: La experiencia y la especialización suponen una cualidad para el conocimiento sobre el manejo del dolor infantil. Los profesionales necesitan más tiempo para la formación especializada y la formación mejora el conocimiento sobre el manejo del dolor infantil.Introduction: The pain sensation is subjective and in the case of the childhood it becomes more complex its valuation due to the difficulties that the children have to communicate. Therefore, he/she couches to undervalue their pain and the treatment is inadequate. This article, it is centered in the professionals of Infirmary that take care to newspaper to children that are subjected to painful situations to be entered or to suffer some alteration of health. The objective is to evaluate the grade of the professionals' of Infirmary knowledge on the infantile pain. Metodology: It is a study pretest-postest cuasiexperimental, in which is distinguished 3 Phases: I: Measured of the grade of knowledge on the pediatric pain by means of a questionnaire already validated. II: formative Intervention to improve the knowledge, the attitude and the abilities necessary for the appropriate handling of the pain. III: Evaluation of the intervention and of the level of knowledge after the same one. Results: The most important global result is that 57% of the professionals possesses a "High" considered level of knowledge and 30% it possesses a "Half" level. This level of knowledge is bigger in the units of Neonatología, and inferior in the of Maternity unidade. Conclusions: The experience and the specialization suppose a quality for the knowledge on the handling of the infantile pain. The professionals need more time for the specialized formation. and the formation improves the knowledge on the handling of the infantile pain

Programa de integración de equipos de trabajo

El objetivo general de este proyecto fue impartir un taller de integración de equipos de trabajo realizando actividades dinámicas con los colaboradores, en donde puedan observar cuáles son sus fortalezas, debilidades y puedan lograr un mejor ambiente de trabajo y una mejor comunicación. El objetivo es que desarrollen y potencialicen sus habilidades comunicativas. El taller engloba los temas de: comunidad de aprendizaje, equipos de trabajo, comunicación y escucha activa. Dicho taller fue impartido a 3 grupos de colaboradores de la empresa. A lo largo de 9 semanas de sesiones, los colaboradores mostraron interés, participación y compromiso para aplicar lo aprendido en su día a día. El 94.44% de los participantes quedaron satisfechos con el taller y redactaron individualmente un compromiso personal para mejorar sus habilidades de comunicación y de esta manera crear un mejor ambiente laboral.ITESO, A.C

Genomic determinants of organohalide-respiration in Geobacter lovleyi, an unusual member of the Geobacteraceae

Background Geobacter lovleyi is a unique member of the Geobacteraceae because strains of this species share the ability to couple tetrachloroethene (PCE) reductive dechlorination to cis-1,2-dichloroethene (cis-DCE) with energy conservation and growth (i.e., organohalide respiration). Strain SZ also reduces U(VI) to U(IV) and contributes to uranium immobilization, making G. lovleyi relevant for bioremediation at sites impacted with chlorinated ethenes and radionuclides. G. lovleyi is the only fully sequenced representative of this distinct Geobacter clade, and comparative genome analyses identified genetic elements associated with organohalide respiration and elucidated genome features that distinguish strain SZ from other members of the Geobacteraceae. Results Sequencing the G. lovleyi strain SZ genome revealed a 3.9 Mbp chromosome with 54.7% GC content (i.e., the percent of the total guanines (Gs) and cytosines (Cs) among the four bases within the genome), and average amino acid identities of 53–56% compared to other sequenced Geobacter spp. Sequencing also revealed the presence of a 77 kbp plasmid, pSZ77 (53.0% GC), with nearly half of its encoded genes corresponding to chromosomal homologs in other Geobacteraceae genomes. Among these chromosome-derived features, pSZ77 encodes 15 out of the 24 genes required for de novo cobalamin biosynthesis, a required cofactor for organohalide respiration. A plasmid with 99% sequence identity to pSZ77 was subsequently detected in the PCE-dechlorinating G. lovleyi strain KB-1 present in the PCE-to-ethene-dechlorinating consortium KB-1. Additional PCE-to-cis-DCE-dechlorinating G. lovleyi strains obtained from the PCE-contaminated Fort Lewis, WA, site did not carry a plasmid indicating that pSZ77 is not a requirement (marker) for PCE respiration within this species. Chromosomal genomic islands found within the G. lovleyi strain SZ genome encode two reductive dehalogenase (RDase) homologs and a putative conjugative pilus system. Despite the loss of many c-type cytochrome and oxidative-stress-responsive genes, strain SZ retained the majority of Geobacter core metabolic capabilities, including U(VI) respiration. Conclusions Gene acquisitions have expanded strain SZ’s respiratory capabilities to include PCE and TCE as electron acceptors. Respiratory processes core to the Geobacter genus, such as metal reduction, were retained despite a substantially reduced number of c-type cytochrome genes. pSZ77 is stably maintained within its host strains SZ and KB-1, likely because the replicon carries essential genes including genes involved in cobalamin biosynthesis and possibly corrinoid transport. Lateral acquisition of the plasmid replicon and the RDase genomic island represent unique genome features of the PCE-respiring G. lovleyi strains SZ and KB-1, and at least the latter signifies adaptation to PCE contamination

Genomic determinants of organohalide-respiration in Geobacter lovleyi, an unusual member of the Geobacteraceae

Background Geobacter lovleyi is a unique member of the Geobacteraceae because strains of this species share the ability to couple tetrachloroethene (PCE) reductive dechlorination to cis-1,2-dichloroethene (cis-DCE) with energy conservation and growth (i.e., organohalide respiration). Strain SZ also reduces U(VI) to U(IV) and contributes to uranium immobilization, making G. lovleyi relevant for bioremediation at sites impacted with chlorinated ethenes and radionuclides. G. lovleyi is the only fully sequenced representative of this distinct Geobacter clade, and comparative genome analyses identified genetic elements associated with organohalide respiration and elucidated genome features that distinguish strain SZ from other members of the Geobacteraceae. Results Sequencing the G. lovleyi strain SZ genome revealed a 3.9 Mbp chromosome with 54.7% GC content (i.e., the percent of the total guanines (Gs) and cytosines (Cs) among the four bases within the genome), and average amino acid identities of 53–56% compared to other sequenced Geobacter spp. Sequencing also revealed the presence of a 77 kbp plasmid, pSZ77 (53.0% GC), with nearly half of its encoded genes corresponding to chromosomal homologs in other Geobacteraceae genomes. Among these chromosome-derived features, pSZ77 encodes 15 out of the 24 genes required for de novo cobalamin biosynthesis, a required cofactor for organohalide respiration. A plasmid with 99% sequence identity to pSZ77 was subsequently detected in the PCE-dechlorinating G. lovleyi strain KB-1 present in the PCE-to-ethene-dechlorinating consortium KB-1. Additional PCE-to-cis-DCE-dechlorinating G. lovleyi strains obtained from the PCE-contaminated Fort Lewis, WA, site did not carry a plasmid indicating that pSZ77 is not a requirement (marker) for PCE respiration within this species. Chromosomal genomic islands found within the G. lovleyi strain SZ genome encode two reductive dehalogenase (RDase) homologs and a putative conjugative pilus system. Despite the loss of many c-type cytochrome and oxidative-stress-responsive genes, strain SZ retained the majority of Geobacter core metabolic capabilities, including U(VI) respiration. Conclusions Gene acquisitions have expanded strain SZ’s respiratory capabilities to include PCE and TCE as electron acceptors. Respiratory processes core to the Geobacter genus, such as metal reduction, were retained despite a substantially reduced number of c-type cytochrome genes. pSZ77 is stably maintained within its host strains SZ and KB-1, likely because the replicon carries essential genes including genes involved in cobalamin biosynthesis and possibly corrinoid transport. Lateral acquisition of the plasmid replicon and the RDase genomic island represent unique genome features of the PCE-respiring G. lovleyi strains SZ and KB-1, and at least the latter signifies adaptation to PCE contamination