Distribution of YLOID in soil-grapevine system (Vitis vinifera L.) as tool for geographical characterization of agro-food products. A two years case study on different grafting combinations

The knowledge of a chemistry relationship between the soil and the agricultural products is an important tool for the quality assessment of food. We studied YLOID (Y, La and lanthanoids), recognized as very useful tracers due their coherent and predictable behavior, to trace and evaluate their distribution from soil to the grape in Vitis vinifera L. Because much of the world’s viticulture is based on grafting, and rootstocks have proved affect vine growth, yield, fruit and wine quality, we carried out experimental trials to analyse the YLOID distribution of two different red cultivars, grafted onto six different rootstocks, on the same soil. The YLOID amounts, the relationship Heavy vs Light YLOID and the pattern of YLOID were calculated. The results showed that the different grafting combinations were not able to induce significant differences in YLOID uptake from the soil maintaining the same fingerprint (with the exception of Eu)

Geochemical behaviour of rare earths in Vitis vinifera grafted onto different rootstocks and growing on several soils

The geochemical behaviour of lanthanides and yttrium(Rare Earth Elements, REEs) has been investigatedmainly in geological systems where these elements represent the best proxies of processes involving the occurrence of an interface between different media. This behaviour is assessed according to features recorded in sequences of REE concentrations along the REE series normalised with respect to a reference material. In this study, the geochemical behaviour of REE was investigated in different parts of Vitis vinifera specimens grown off-soil, on soils of different nature and grafted onto several rootstocks in order to evaluate effects induced by these changes. The results indicated that roots are the plant organs where REEs are preferentially concentrated, in particular elements from Sm to Ho (middle REE, MREE) whereas Eu enrichments occur in aerial parts. The geochemical behaviour of REE suggests that MREE enrichments in roots are due to preferential MREE interactions with biological membranes or to surface complexation with newly formed phosphates. Eu-positive anomalies suggest that Eu3+ can form stable organic complexes in place of Ca2+ in several biological processes in xylem fluids. The possibility that Eu mobility in these fluids can be enhanced by its reductive speciation as Eu2+ cannot be ruled out. The assessment of the geochemical behaviour of REE according to the theory of the Tetrad Effect carried out confirms that REEs coming fromsoil are scavenged onto root tissues or mineral surfaceswhereas their behaviour in aerial parts of V. vinifera is driven by dissolved complexation

Combined influence of bud load and bud position along the cane on vegetative and reproductive parameters of grape cv. Grillo

Cultivar Grillo vines are characterized by problems with flower biology (the fertility of basal buds) and fructification (millerandage). In this study, to manage the variability in bunch weight with winter pruning and to program others canopy management practices (i.e. early defoliation), three different treatments of bud load were set up by leaving the cane with 3, 6 or 10 buds. The effects of bud load and cane length were studies regarding bud fertility, shoot leaf area, and the number of flowers and berries, as well as the relationship between leaf area and percentage of fruit set, leaf area/flower and percentage of fruit set, and the number of hens and chicks berries. Shoots in the distal position had higher values of fertility and inflorescences with a greater number of flowers, while no ‘apical’ effect of the buds emerged. A good relationship was found between fruit set and the number of flowers, leaf area at flowering and yield, and square centimeters/flower and percentage of fruit set. Cane length was found to be a valid tool for managing bunch weight variability; the value of the leaf area/flower can be used to program early defoliation practice carried out to manage berry set

The impact of soil erosion on soil fertility and vine vigor. A multidisciplinary approach based on field, laboratory and remote sensing approaches

Soil erosion processes in vineyards, beyond surface runoff and sediment transport, have a strong effect on soil organic carbon (SOC) loss and redistribution along the slope. Variation in SOC across the landscape can determine differences in soil fertility and vine vigor. The goal of this research was to analyze the interactions among vines vigor, sediment delivery and SOC in a sloping vineyard located in Sicily. Six pedons were studied along the slope by digging 6 pits up to 60 cmdepth. Soilwas sampled every 10 cm and SOC, water extractable organic carbon (WEOC) and specific ultraviolet absorbance (SUVA) were analyzed. Erosion rates, detachment and deposition areas were measured by the pole height method which allowed mapping of the soil redistribution. The vigor of vegetation, expressed as Normalized Difference Vegetation Index (NDVI), derived from highresolution satellite multispectral data, was compared with measured pruning weight. Results confirmed that soil erosion, sediment redistribution and SOC across the slope was strongly affected by topographic features, slope and curvature. The erosion ratewas 16Mg ha−1 y−1 since the timeof planting (6 years). SOC redistribution was strongly correlated with the detachment or deposition areas as highlighted by pole height measurements. The off-farm SOC loss over six years amounted to 1.2 Mg C ha−1. SUVA254 values, which indicate hydrophobic material rich in aromatic constituents of WEOC, decreased significantly along the slope, demonstrating that WEOC in the detachment site ismore stable in comparison to deposition sites. The plant vigor was strongly correlated with WEOC constituents. Results demonstrated that high resolution passive remote sensing data combined with soil and plant analyses can survey areas with contrasting SOC, soil fertility, soil erosion and plant vigor. Thiswill allowmonitoring of soil erosion and degradation risk areas and support decision-makers in developing measures for friendly environmental management

Quality of Grapes Grown Inside Paper Bags in Mediterranean Area

The aim of this study was to evaluate the influence of paper bagging of grape bunches on the morphological, mechanical, and chemical characteristics of berries of three table grapes varieties as an environmentally‐friendly technique for protecting clusters from biotic and abiotic agents. Clusters of Italia, Autumn Royal, and Regal Seedless grape cultivars were bagged and compared to a not‐bagged control. Air temperature inside and outside the bags was monitored. Bunch weight and length, number of berries per bunch, berry longitudinal and transversal diameter, berry mass, number of seeds per berry (normal in size and aborted), soluble solid content, titratable acidity, and skin color by CIEL*a*b* parameters were determined on four points of each berry. Berries were evaluated using texture analysis, and the main texture profile analysis parameters were compared. The air temperatures around not‐bagged clusters were slightly higher than inside the bags. In all the cultivars under evaluation, bagged bunches were heavier compared with not‐bagged ones. In Autumn Royal and Regal Seedless, these differences were mainly owing to the higher number of berries and higher berry weight of the bagged bunches. Regarding mechanical properties, in seedless varieties, the bagging treatment increased berry hardness (related to the berry firmness) and decreased berry cohesiveness and resilience, whereas an opposite behavior was found in cv. Italia. Berry skin break force was lower in the bagging treatment in all the analyzed varieties, indicating a softer and easier‐to‐chew berry skin. The findings demonstrate that the bagging technique affected the three variety parameters to different extents. The main differences were found in the seedless varieties in terms of berry size and bunch characteristics. For all varieties, bagged bunches achieved the quality level required by the market, confirming the suitability of this technique. However, the bag industry is proposing many different bag types (differing in material, shape, color, and closing system); therefore, further studies are needed to obtain more complete and exhaustive technical information

A novel infrastructure for the continuous monitoring of soil CO2 emissions: a case study at the alto Tiberina near fault observatory in Italy

Static and dynamic stress, along with earthquakes, can trigger the emission and migration of crustal fluids, as frequently observed on the surface and within the upper crust of tectonically active areas such as the northern Apennines of Italy. To investigate the origin of these fluids and their interconnection with the seismogenic process, we complemented The Alto Tiberina Near Fault Observatory (TABOO-NFO), a multidisciplinary monitoring infrastructure composed of a dense array of seismic, geodetic, strain, and radon sensors, with a proper geochemical network grounded on four soil CO2 flux monitoring stations and weather sensors, placed near the main vents of the superficial manifestations. The TABOO-NFO is a state-of-the-art monitoring infrastructure, which allows for studying various geophysical parameters connected to the deformation processes active along a crustal fault system dominated by the Alto Tiberina fault (ATF), which is a 60 km long normal fault dipping at a low angle (<15°–20°). The region is favourable for conducting geochemical studies, as it is characterised by the presence of over-pressurised fluids trapped at certain depths and superficial manifestations associated with the emission of large quantities of fluids. After describing the theoretical framework and the technological aspects based on which we developed the geochemical monitoring network, we described the data recorded in the first months. Over the studied period, the results showed that soil CO2 flux was primarily influenced by environmental parameters, and that the selected sites received a regular supply of deep-origin CO2

MORPHOLOGICAL ANALYSIS OF ANCIENT GRAPE SEEDS FROM A SINK IN THE MIDDLE-AGE TOWN OF PALERMO

The archaeological excavations in Piazza della Vittoria, in the Roman-Middle Age town of Palermo (Sicily) put in light a sink 3.20 m deep and 1 square m. large, partially filled by thin organic sediments. Grape seeds (grape-stones), fish scales and few vertebrate bones have been found in specific strata sealed under a stratum chronologically attributed to Islamic Middle-Age period (a post-quem limit). The finding of well preserved grape seeds is peculiar and their study opens the opportunity to improve the actual knowledge about evolution, cultivation, use and trade of Vitis L. in the Mediterranean area. This preliminary work focuses on morphologic and morphometric analysis of the ancient grape seeds with two aims: i) systematically describe the remains collection and, ii) define seeds typology and a consequent morphotaxonomic attribution. Over 200 seeds have been carefully dry cleaned (soft brush), photographed and analyzed for total breadth (B), total length (L) and length of stalk (LS) parameters, the most efficient for typological attribution (1, 2); Stummer index has been also calculated (1). Apical notch length (AN) has been for the first time evaluated. Measurements on digital images have been performed using ImageJ 1.31 platform; morphological parameters have been assembled in a dedicated database. Descriptive analysis and linear correlations have been performed using SYSTAT 10. Analysis of variance (ANOVA) and Tukey’s HSD (5% level of significance, α = 0.05) have been applied. All the parameters approximate a normal distribution. Major variation has been observed in LS (c.v. = 35.6%) and AN (c.v. = 35.6%), while B and L showed a c.v. of 9.5% and 12.6% respectively. All the analyzed parameters behave as independent variables with the exception of a significant correlation between Stummer index and L (R2 = 0.45; y = 8.17-0.047x with y = L and x = Stummer index). This correlation reveals that Stummer index depends more from the L and not from the B parameter. On the base of LS measures three subgroups have been arbitrarily created in relationship with the LS: LS1 0.90 mm (35 seeds). Analyzing together the LS groups toward AN, we have found a proportional and significant correlation (p = 0.05) between the extremes LS1 and LS3. In the entire collection, Stummer index varies from 55.76 to 100.86; in the LS groups, the range is 68.38-97.87 in LS1, 61.02-100.86 in LS2 and 55.76-81.70 in LS3. A small group (17) of seeds has been excluded for the impossibility to measure the stalk. The analyzed ancient grape seeds show a wide range of variability for all the considered parameters, revealing a polymorphic collection. In general, the seeds have a rounded heart-like shape, with a noticeable pointy stalk and a very invaginated apical notch. This typical shape is more marked in LS3 group. On the base of LS measures, LS1 is ascribable to wild grapevines, while LS2 and LS3 seem to be ascribable neither to wild nor to cultivated autochthonous Vitis. Furthermore, these seeds differ from those already described in other archaeological horizons in Italy (3) and in France (2). The Stummer index varies highly, exceeding the known range of wild Vitis vinifera (4), although values close to 100 have been already found in wild grapevines in Spain (5) and values above 80 have been also described in Extra-European Vitis species (6). A deep evaluation of the sample, including isotopic analysis and aDNA studies, is in progress. 1) T. T. Korenčič, J. Jakše, Z. Korošec-Koruza (2008) Veget. Hist. Archaeobot., 17(Suppl. 1), S93-S102 2) L. Bouby, I. Figueiral, A. Bouchette, N. Rovina, S. Ivorra, T. Lacombe, T. Pastor, S. Picq, P. Marinval, J. F. Terral (2013) PLoS ONE, 8(5), e63195 3) C. Milanesi, F. Antonucci, P. Menesatti, C. Costa, C. Faleri, M. Cresti (2011) Interdisciplinaria Archaeologica – Natural Sciences in Archaeology, II(2), 95-100 4) A. Stummer (1911) Mitt. Anthropol. Gesellschaft Wien, 41, 283-296 5) F. M. De Toda, J. C. Sancha (1999) Am. J. Enol. Vitic., 50(4), 443-446 6) D. Rivera, B. Miralles, C. Obón, E. Carreño, J. A. Palazón (2007) Vitis, 46(4), 158-16

Effect of solid waste landfill organic pollutants on groundwater in three areas of Sicily (Italy) characterized by different vulnerability

The aim of this study was to obtain information on the presence and levels of hazardous organic pollutants in groundwater located close to solid waste landfills. Eighty-two environmental contaminants, including 16 polycyclic aromatic hydrocarbons (PAHs), 20 volatile organic compounds (VOCs), 29 polychlorinated biphenyls (PCBs), 7 dioxins (polychlorinated dibenzo-p-dioxins, PCDDs) and 10 furans (polychlorinated dibenzofurans, PCDFs) were monitored in areas characterised by different geological environments surrounding three municipal solid waste landfills (Palermo, Siculiana and Ragusa) in Sicily (Italy) in three sampling campaigns. The total concentrations of the 16 PAHs were always below the legal threshold. Overall, the Fl/Fl + Py diagnostic ratio revealed that PAHs had a petrogenic origin. VOC levels, except for two notable exceptions near Palermo landfill, were always below the legal limit. As concerns PCB levels, several samples were found positive with levels exceeding the legal limits. It is worth noting that the % PCB distribution differs from that of commercial compositions. In parallel, some samples of groundwater containing PCDDs and PCDFs exceeding the legal threshold were also found. Among the 17 congeners monitored, the most abundant were the highest molecular weight ones.Published16869 – 168826V. Pericolosità vulcanica e contributi alla stima del rischioJCR Journa

Spectrum of mutations in Italian patients with familial hypercholesterolemia: New results from the LIPIGEN study

Background Familial hypercholesterolemia (FH) is an autosomal dominant disease characterized by elevated plasma levels of LDL-cholesterol that confers an increased risk of premature atherosclerotic cardiovascular disease. Early identification and treatment of FH patients can improve prognosis and reduce the burden of cardiovascular mortality. Aim of this study was to perform the mutational analysis of FH patients identified through a collaboration of 20 Lipid Clinics in Italy (LIPIGEN Study). Methods We recruited 1592 individuals with a clinical diagnosis of definite or probable FH according to the Dutch Lipid Clinic Network criteria. We performed a parallel sequencing of the major candidate genes for monogenic hypercholesterolemia (LDLR, APOB, PCSK9, APOE, LDLRAP1, STAP1). Results A total of 213 variants were detected in 1076 subjects. About 90% of them had a pathogenic or likely pathogenic variants. More than 94% of patients carried pathogenic variants in LDLR gene, 27 of which were novel. Pathogenic variants in APOB and PCSK9 were exceedingly rare. We found 4 true homozygotes and 5 putative compound heterozygotes for pathogenic variants in LDLR gene, as well as 5 double heterozygotes for LDLR/APOB pathogenic variants. Two patients were homozygous for pathogenic variants in LDLRAP1 gene resulting in autosomal recessive hypercholesterolemia. One patient was found to be heterozygous for the ApoE variant p.(Leu167del), known to confer an FH phenotype. Conclusions This study shows the molecular characteristics of the FH patients identified in Italy over the last two years. Full phenotypic characterization of these patients and cascade screening of family members is now in progress