Notulae To The Italian Native Vascular Flora: 11

In this contribution, new data concerning the distribution of native vascular flora in Italy are presented. It includes new records, confirmations, exclusions, and status changes to the Italian administrative regions. A new combination in the genus Pilosella is proposed. Nomenclatural and distribution updates, published elsewhere, and corrigenda are provided as Suppl. material 1

Development of High-Specificity Fluorescent Probes to Enable Cannabinoid Type 2 Receptor Studies in Living Cells

Pharmacological modulation of cannabinoid type 2 receptor (CB2R) holds promise for the treatment of numerous conditions, including inflammatory diseases, autoimmune disorders, pain, and cancer. Despite the significance of this receptor, researchers lack reliable tools to address questions concerning the expression and complex mechanism of CB2R signaling, especially in cell-type and tissue-dependent context. Herein, we report for the first time a versatile ligand platform for the modular design of a collection of highly specific CB2R fluorescent probes, used successfully across applications, species and cell types. These include flow cytometry of endogenously expressing cells, real-time confocal microscopy of mouse splenocytes and human macrophages, as well as FRET-based kinetic and equilibrium binding assays. High CB2R specificity was demonstrated by competition experiments in living cells expressing CB2R at native levels. The probes were effectively applied to FACS analysis of microglial cells derived from a mouse model relevant to Alzheimer’s disease and to the detection of CB2R in human breast cancer cells

Artificial Intelligence at the JRC: 2nd workshop on Artificial Intelligence at the JRC, Ispra 5th July 2019

This document presents the contributions discussed at the second institutional workshop on Artificial Intelligence (AI), organized by the Joint Research Centre (JRC) of the European Commission. This workshop was held on 05th July 2019 at the premises of the JRC in Ispra (Italy), with video-conference to all JRC's sites. The workshop aimed to gather JRC specialists on AI and Big Data and share their experience, identify opportunities for meeting EC demands on AI, and explore synergies among the different JRC's working groups on AI. In comparison with the first event, according to the JRC Director General Vladimír Šuchav, the activities and results presented in this second workshop demonstrated a significant development of AI research and applications by JRC in different policy areas. He suggested to think about replicating the event at the premises of diverse policy DGs in order to present and discuss the clear opportunities created by JRC activities. After the opening speech by the JRC Director General Vladimír Šuchav, the research and innovation presentation were anticipated by two presentations by Alessandro Annoni and Stefano Nativi. The first presentation dealt with the results of one year of AI@JRC and six months of fully operational AI&BD community of practice1. The second presentation reported the results of the AI competences survey at JRC. The research and innovation contributions consisted in flash presentations (5 minutes) covering a wide range of areas. This report is structured according to the diverse domain areas addressed by the presenters. While the first part of the workshop was mainly informative, in the second part we collectively discussed about how to move on and evolve the AI&BD community of practice.JRC.B.6-Digital Econom