Mycobacterium avium Complex in domestic and wild animals

Mycobacteria from the Mycobacterium avium complex (MAC) cause a variety of diseases including tuberculosis-like disease in humans and birds, disseminated infections in AIDS patients and otherwise immunocompromised patients, lymphadenitis in humans and mammals and paratuberculosis in ruminants. M. avium subsp. paratuberculosis (Map) is the etiologic agent of Johne´s disease in cattle and it has been identified in human patients with Crohn’s disease. The MAC comprises slow growing mycobacteria that are ubiquitous in the environment (soil and water), and have a wide source range, causing disease in various domestic and wild mammals and birds [1]. The aim of this study was to discuss the classification and biology, epidemiology, clinical signs, pathology, diagnostic techniques, and public health concerns in Mycobacterium avium complex in domestic and wild animals

Lymphocyte population in the granulomatous lesions of wild-boars (Sus scrofa) suspected of tuberculosis

Só está disponível o resumoLymphocyte population in the granulomatous lesions of wild-boars (Sus scrofa) suspected of tuberculosis

PERFIL LONGITUDINAL E A APLICAÇÃO DO ÍNDICE DE GRADIENTE (RDE) NA BACIA HIDROGRÁFICA DO ALTO CURSO DO RIO PITANGUI – PR.

Este trabalho apresenta uma análise do perfil longitudinal e da aplicação do índice de gradiente (RDE) no Alto curso do Rio Pitangui - PR, com o objetivo de detectar anomalias de drenagem ao longo de seu curso principal. Este rio possui uma extensão de 42,384 km e amplitude de relevo de 156 m. Em sua maior parte, o canal encontra-se desajustado, sendo que esses trechos estão associados a mudanças litológicas, lineamentos do relevo e encontro de tributários significativos

Importância dos Saca-Rabos (Herpestes Ichneumon) como Reservatório de Mycobacterium avium subsp. paratuberculosis. Deteção por Técnicas Tradicionais e Moleculares

Poster apresentado nas IV Jornadas de Genética, realizadas na UTAD, Vila Real, nos dias 1,2 e 3 de Março de 2012.Os saca-rabos (Herpestes ichneumon) também conhecidos por mangustos, são carnívoros diurnos selvagens que juntamente com a geneta (Genetta genetta) representam os exemplares da família Viverridae em Portugal. É uma espécie cinegética de caça menor que se alimenta de coelhos, roedores, aves, cobras, insectos e ovos. Neste estudo colheram-se amostras de 8 animais mortos por atropelamento e em ações de controlo de predadores, durante os anos de 2010 e 2011, nos concelhos de Idanha-a-Nova e Penamacor do distrito de Castelo Branco. As amostras colhidas foram fígado, pulmão, baço, intestino, rim, gânglio mesentérico, retrofaríngeo, mediastínico, amígdalas e fezes. As amostras foram submetidas à técnica de PCR e a cultura microbiológica em meios específicos. Em três saca-rabos (37,5%) detectou-se Mycobacterium avium subsp. paratuberculosis (Map) através da técnica de biologia molecular. Dois eram machos e um era fêmea. Map foi confirmado também em cultura nos dois machos. Sete saca-rabos (87,5%) apresentaram bactérias álcool-ácido resistentes compatíveis com Map em esfregaços de diferentes tecidos, quando corados pelo método de Ziehl-Neelsen. Estes resultados preliminares confirmam os saca-rabos como reservatório de Map no nosso país. Atualmente, estão a ser desenvolvidos mais estudos para a avaliação dos saca-rabos na dinâmica da infeção de Map em mamíferos selvagens

Pulmonary lesions consistent with disseminated adiaspiromycosis in egyptian mongooses (Herpestes ichneumon) from Portugal.

Pulmonary lesions consistent with disseminated adiaspiromycosis in egyptian mongooses (Herpestes ichneumon) from Portugal

Serosurvey of Mycobacterium avium Complex in wild boars in Portugal

Serosurvey of Mycobacterium avium Complex in wild boars in Portugal

Deteção molecular de Mycobacterium avium subsp. paratuberculosis em duas lontras (Lutra lutra, Linnaeus, 1758)

Deteção molecular de Mycobacterium avium subsp. paratuberculosis em duas lontras (Lutra lutra, Linnaeus, 1758)

Development of triamcionolone based lipid nanocapsules as platforms for ocular drug delivery

Purpose Triamcinolone acetonide (TAA) is considered a first-line drug by itself or as a combined treatment of several intraocular diseases such as macular edema, retinal vein thrombosis, uveitis and age-related macular degeneration. The development of TAA dosage forms is limited due to its poor solubility in water and physiologically acceptable solvents. Lipid nanocapsules (LNCs) are biocompatible systems that allow loading both hydrophobic and hydrophilic drugs. LNCs present a versatile composition and application suitable for different routes of administration. The aim of this work was to develop and characterize a novel lipid LNCs formulation containing TAA as drug delivery system. Methods LNCs were prepared in triplicate using an optimized phase inversion-based method described by Heurtault et al., 2002. Due to the poor solubility of TAA in the oily phase of the original formulation, two co-surfactants (captex® 500p -Glyceryl triacetate and oleic acid) in three proportions (20, 30 and 50%) were tested. The average particle size (APS), polydispersity index (PI), zeta potential (ZP) and entrapment efficacy (EE) were measured. Results Acceptable results were obtained with a 20% of both co-surfactants. LNCs with captex® 500p leads to about (40±1) nm size nanoparticles with a narrow size distribution (PI less than 0.2), a negative ZP (-1.2±0.7) mV and EE (85.8±0.8) % while LNCs with oleic acid showed an APS of (35.9± 0.6) nm and a PI below 0.1 with a negative ZP (-3.6±0.6) mV and EE (87±2) %. Moreover, both systems were stable for two months. Conclusions LNCs allow encapsulation of TAA and their properties remain constant over long periods of time. Thus, LNCs are promising systems than may be a potential strategy to improve efficacy and decrease side effects of this drug so used in the treatment of intraocular diseases