Electrochemical detection of in situ adriamycin oxidative damage to DNA

Adriamycin intercalation and in situ interaction with double helix DNA was investigated using a voltammetric DNA-biosensor. Oxidation and reduction of adriamycin molecules intercalated in double helix DNA were investigated in order to understand the in vivo mechanism of action with this anti-neoplasic drug. The results showed that the interaction of adriamycin with DNA is potential-dependent causing contact between DNA guanine and adenine bases and the electrode surface such that their oxidation is easily detected. A mechanism for adriamycin reduction and oxidation in situ when intercalated in double helix DNA immobilised onto the glassy carbon electrode surface is presented and the formation of the mutagenic 8-oxoguanine explained.http://www.sciencedirect.com/science/article/B6THP-44PVS7R-M/1/1474d369a4b1d556b84d548d2df2543

Population structure of Pentaclethra macroloba (Willd.) Kuntze in high and low floodplains of the Amazonian estuary.

The flood cycle of floodplain forests and topographic variations are important factors of diversification and morphological and ecophysiological adaptations of the plant community. Pentaclethra macroloba is a hyperdominant species adapted to flood variations. The objective of this study was to characterize the P. macroloba population structure between environments with high topography, flooded only during the rainy season and peak flood of the river, (high várzea-HV) and low topography with daily flood (low várzea-LV). The study was carried in the Mazagão Experimental Field, Brazil. Four plots of 1 ha were installed in each environment. All individuals with DBH ≥ 5 cm were measured and georeferenced. ANOVA test, 0.05% probability, was applied to verify the structural differences between two environments. The HV had higher population density (51.2 individuals.ha-1) compared to the LV (36.7 individuals.ha-1), although without significance. Basal area was higher in the HV (10.42 m²) in comparison with LV (5.90 m²), with significant difference (F = 7.13, p = 0.00795). The maximum DAP of HV was 67 cm with mean of 24 cm. The LV presented maximum DBH of 42 cm with mean of 21 cm, showing structural difference between the two environments (F = 6.22, p = 0.0131). The aggregation index showed high aggregation in the LV (R = 0.89) than in the HV (R = 0.79), differing significantly between the two environments (F = 11.31, p = 0.0008). Frequency and time of flooding are ecological drives that structure the population of pracaxizeiro causing morphological and ecophysiological adaptations to the individual

Diagnóstico fitossanitário em sementes de cedro e tachi branco.

O trabalho objetivou diagnosticar fitopatógenos presentes em sementes de cedro vermelho (Cedrela odorata L.) e tachi branco (Sclerolobium paniculatum Vogel), espécies florestais nativas da Amazônia. A importância da patologia de sementes relaciona-se com a disseminação de doenças pelo fruto quando este é levado para beneficiamento e para evitar o apodrecimento das sementes, o que pode ocasionar perda pela presença de fitopatógenos nas mesmas. Os testes foram feitos com base nas Regras de Análise de Sementes (RAS) do Ministério da Agricultura, Pecuária e Abastecimento ? MAPA, pelo método de incubação em papel filtro ?Blotter Test?. Foram detectados os seguintes gêneros de fungos: Aspergillus sp., Penicillium spp. e Lasiodiplodia sp. em sementes de cedro e Penicillium spp. e Aspergillus sp. em tachi branco. As sementes de cedro apresentaram maiores níveis de incidência de fungos.PIBIC-2011

Synthesis of new donor/acceptor 5-cyclopentadienyl and 5-indenyliron(II) complexes with p-benzonitrile derivatives. Crystal structures of [Fe( 5-C5H5)(CO)(P(OC6H5)3)(p-NCC6H4NO2)][BF4]·CH2Cl2 and [Fe( 5-C9H7)(CO)(P(OC6H5)3)(p-NCC6H4NO2)][BF4]

New complexes of the type [Fe( 5-Cp or Ind)(L)(L )(p-NCR)][BF4] (L, L =CO, P(OC6H5)3, P(C6H5)3; R=C6H4N(CH3)2, C6H4NO2, (E)-C(H) C(H)C6H4NO2, (E)-C(H) C(H)C6H4N(CH3)2) have been synthesised and characterised. Spectroscopic data were analysed in order to evaluate the tuning of the electronic density at the metal centre and the extension of the -delocalisation on the molecule, due to the presence of coligands with different acceptor/donor abilities. The structures of two complexes [Fe( 5-C5H5)(CO)(P(OC6H5)3)(p-NCC6H4NO2)][BF4] and [Fe( 5-C9H7)(CO)(P(OC6H5)3)(p-NCC6H4NO2)][BF4] were determined by X-ray crystallographic analysis. The compounds crystallised in the centrosymmetric space groups P1 and P21/n, respectively. Bond distances within the nitrile ligand are discussed in order to evaluate the nature of iron–nitrile bonding in these complexes

Variabilidade de isolados de Xanthomonas axonopodis pv. passiflorae no estado do Pará.

Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 797

River damming affects seedling communities of a floodplain forest in the Central Amazon

The flood pulse of black water rivers in the Amazon basin determines the composition of species along the flood gradient in igapó forests. The Balbina dam, built on the Uatumã River, has altered the flood pulse and caused changes in the floristic composition of adult trees throughout the downstream area. There is a lack of studies on how communities of seedlings in igapó forests respond to changes in the flood pulse. This study investigates the response of seedling communities in the igapó forest downstream the Balbina dam and compares it with two pristine areas. The areas were sampled with transects of 1x25 m within 36 plots (25x25 m) along the flood gradient. Richness and dominance were calculated by simple regression and ordination analyses. The pristine areas had the same pattern of richness, dominance and genera distribution along the flood gradient. However, the affected Uatumã area formed different groups of genera by NMDS analysis, which divided them along the flood gradient with significantly increased dominance of three genera. The insertion of the Balbina dam resulted a loss of lateral and longitudinal connectivity for the Uatumã River, and the alteration to seedling communities may alter the future landscape of downstream igapó forests

Extratos aquosos de Artocarpus heterophyllus Lam. e Morinda citrifolia Linn no controle da mancha bacteriana do maracujazeiro.

Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 1244

Phenological behavior and foral visitors of Pentaclethra macroloba, a hyperdominant tree in the Brazilian Amazon River estuary.

Natural history of hyperdominant tree populations in the Brazilian Amazon Region is still unknown in plant science. Pentaclethra macroloba (Willd.) Kuntze (Fabaceae) is a hyperdominant tree species which has multiple uses and its seeds are extensively explored to extract medicinal oil. We evaluated the phenological cycles and floral visitors of P. macroloba and the effect of the daily tide of the Amazon River on the phenophases. Phenophases of flower bud, anthesis flowers, immature fruit, ripe fruit, seed dispersal, new leaf, mature leaf, and leaf fall of 29 trees in the Northeast of the Brazilian Amazon were monitored during 28 months. Hydrometeorological data of rainfall, maximum temperature and flood height on the tree trunk were obtained. Generalized Linear Models were used to explain the relationship between phenophases and hydrometeorological parameters. Flowering was synchronized in the dry season (flower buds: βtemperature = 1.30, p < 0.01 and flowers in anthesis: βtemperature = 1.84; p < 0.001). Immature fruits appeared during the dry season (βtemperature = 0.67; p < 0.01) and ripe fruits in the rainy season. Seeds were dispersed during the rainfall (βrainfall = 0.0051; p < 0.01) and flooding of the river (βwater = 0.12; p < 0.001). There was no relationship between leaf change and hydrometeorological variables. The main floral visitors were wasps, bees and ants. The rainfall seasonality is a key factor that stimulates reproductive events of P. macroloba. Daily river flooding can be considered a driver that stimulates the tree to disperse its seeds at the peak of the river flood, where they can be transported the long distances

Sincronização folicular e vascularização do folículo dominante em novilhas mestiças tratadas com estradiol.

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