The "Maggie" filament: Physical properties of a giant atomic cloud

The atomic phase of the interstellar medium plays a key role in the formation process of molecular clouds. Due to the line-of-sight confusion in the Galactic plane that is associated with its ubiquity, atomic hydrogen emission has been challenging to study. Employing the high-angular resolution data from the THOR survey, we identify one of the largest, coherent, mostly atomic HI filaments in the Milky Way at the line-of-sight velocities around -54 km/s. The giant atomic filament "Maggie", with a total length of 1.2 kpc, is not detected in most other tracers, and does not show signs of active star formation. At a kinematic distance of 17 kpc, Maggie is situated below (by 500 pc) but parallel to the Galactic HI disk and is trailing the predicted location of the Outer Arm by 5-10 km/s in longitude-velocity space. The centroid velocity exhibits a smooth gradient of less than $\pm$3 km/s /10 pc and a coherent structure to within $\pm$6 km/s. The line widths of 10 km/s along the spine of the filament are dominated by non-thermal effects. After correcting for optical depth effects, the mass of Maggie's dense spine is estimated to be $7.2\times10^5\,M_{\odot}$. The mean number density of the filament is 4$\rm\,cm^{-3}$, which is best explained by the filament being a mix of cold and warm neutral gas. In contrast to molecular filaments, the turbulent Mach number and velocity structure function suggest that Maggie is driven by transonic to moderately supersonic velocities that are likely associated with the Galactic potential rather than being subject to the effects of self-gravity or stellar feedback. The column density PDF displays a log-normal shape around a mean of $N_{\rm HI} = 4.8\times 10^{20}\rm\,cm^{-2}$, thus reflecting the absence of dominating effects of gravitational contraction

Two-Photon Microscopy for Non-Invasive, Quantitative Monitoring of Stem Cell Differentiation

BACKGROUND: The engineering of functional tissues is a complex multi-stage process, the success of which depends on the careful control of culture conditions and ultimately tissue maturation. To enable the efficient optimization of tissue development protocols, techniques suitable for monitoring the effects of added stimuli and induced tissue changes are needed. METHODOLOGY/PRINCIPAL FINDINGS: Here, we present the quantitative use of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) as a noninvasive means to monitor the differentiation of human mesenchymal stem cells (hMSCs) using entirely endogenous sources of contrast. We demonstrate that the individual fluorescence contribution from the intrinsic cellular fluorophores NAD(P)H, flavoproteins and lipofuscin can be extracted from TPEF images and monitored dynamically from the same cell population over time. Using the redox ratio, calculated from the contributions of NAD(P)H and flavoproteins, we identify distinct patterns in the evolution of the metabolic activity of hMSCs maintained in either propagation, osteogenic or adipogenic differentiation media. The differentiation of these cells is mirrored by changes in cell morphology apparent in high resolution TPEF images and by the detection of collagen production via SHG imaging. Finally, we find dramatic increases in lipofuscin levels in hMSCs maintained at 20% oxygen vs. those in 5% oxygen, establishing the use of this chromophore as a potential biomarker for oxidative stress. CONCLUSIONS/SIGNIFICANCE: In this study we demonstrate that it is possible to monitor the metabolic activity, morphology, ECM production and oxidative stress of hMSCs in a non-invasive manner. This is accomplished using generally available multiphoton microscopy equipment and simple data analysis techniques, such that the method can widely adopted by laboratories with a diversity of comparable equipment. This method therefore represents a powerful tool, which enables researchers to monitor engineered tissues and optimize culture conditions in a near real time manner

Hearing and dementia

Hearing deficits associated with cognitive impairment have attracted much recent interest, motivated by emerging evidence that impaired hearing is a risk factor for cognitive decline. However, dementia and hearing impairment present immense challenges in their own right, and their intersection in the auditory brain remains poorly understood and difficult to assess. Here, we outline a clinically oriented, symptom-based approach to the assessment of hearing in dementias, informed by recent progress in the clinical auditory neuroscience of these diseases. We consider the significance and interpretation of hearing loss and symptoms that point to a disorder of auditory cognition in patients with dementia. We identify key auditory characteristics of some important dementias and conclude with a bedside approach to assessing and managing auditory dysfunction in dementia

Internet-based interventions for adults with hearing loss, tinnitus and vestibular disorders: protocol for a systematic review

Background: Internet-based interventions are emerging as an alternative way of delivering accessible healthcare for various conditions including hearing and balance disorders. A comprehensive review regarding the evidence-base of Internet-based interventions for auditory-related conditions is required to determine the existing evidence of their efficacy and effectiveness. The objective of the current protocol is to provide the methodology for a systematic review regarding the effects of Internet-based interventions for adults with hearing loss, tinnitus and vestibular disorders. Method: This protocol was developed according to the Preferred Reporting Items for Systematic reviews and Meta-analyses for Protocols (PRISMA-P) 2015 guidelines. Electronic database searches will include EBSCOhost, PubMed and Cochrane Central Register performed by two researchers. This will be complemented by searching other resources such as the reference lists for included studies to identify studies meeting the eligibility for inclusion with regard to study designs, participants, interventions, comparators and outcomes. The Cochrane risk of bias tool (RoB 2) for randomised trials will be used for the bias assessments in the included studies. Criteria for conducting meta-analyses were defined. Discussion: The result of this systematic review will be of value to establish the effects of Internet-based interventions for hearing loss, tinnitus and vestibular disorders. This will be of importance to guide future planning of auditory intervention research and clinical services by healthcare providers, researchers, consumers and stakeholders