Effect of two sous-vide cooking methods on physicochemical characteristics of Longissimus thoracis muscle from pigs fed with or without extruded linseed

Meat is recognised as a highly nutritive food. With cooking, the meat enhances its nutritional value and becomes more digestible. However, high cooking temperatures lead to several chemical modifications in meat. Therefore, there is an increasing interest in sous-vide cooking. The aim of this research was to study the influence of two sous-vide cooking methods on physicochemical characteristics of Longissimus thoracis (LT) muscle from 24 pigs fed with two different diets: control group (C) had a basal barley/ soybean diet; in the linseed group (L), 5% of extruded linseed replaced the same amount of barley. At 24 h post mortem, LT muscles were sliced, vacuum sealed and stored at −18 °C until analysis. Samples were cooked in water bath at two different combinations of temperature and time: ‘A’ 80 °C of the bath water as long as the core temperature of the pork reached 70 °C; ‘B’ 60 °C for 15 h. After the cooking process, the samples were kept under refrigeration (2 °C) for 24 h. The day after, cooking loss, colour, pH, microbial growth and tenderness were determined. Microbial growth was analysed also in the raw meat. Statistical analysis was performed by means of ANOVA, using the GLM procedure of SAS. Dietary treatment (C vs. L) and cooking condition (A vs. B) were used as independent variables. Dietary treatments did not produce significant differences in pH, colour, cooking loss and tenderness. Colour parameters were affected by cooking method: a* values of the internal part of the sample and b* values of the external part were higher for the samples cooked at 60 °C (a* 4.74 vs. 3.97 for B and A, respectively, p<.05; b* 17.79 vs. 15.84, p<.01). The A cooking method led to higher (p<.01) shear force values (5.03 vs. 3.30 kg). The microbial load in the raw meat was significantly different (p<.05) between dietary treatments: C group showed higher total viable count (4.56 vs. 4.14 log10) and Enterobacteriaceae (2.65 vs. 1.94 log10) respect to L group. Low microbial growth was detected for both cooking methods. Total viable count was 0.50 log10 for A cooking method and 0.64 log10 for B, without significant differences among them. No Enterobacteriaceae growth was detected. Also, no differences were found for pH and cooking loss between A and B. Both cooking methods generated a meat safe from contamination. The B method: low temperature for long time also, generated tender meat

CHARACTERISTICS OF LIPIDS FROM IMMUNOCASTRATED MEDIUM-HEAVY PIGS FED EITHER A RESTRICTED DIET OR AD LIBITUM

We studied the feeding level-related variations in lipid characteristics in the adipose tissues of pigs. The lipid content, fatty acid profile, oxidative stability, iodine value, thrombogenic and atherogenic indices were determined in individual samples from 24 immunocastrated males (Duroc x Large White), fed either restricted or ad libitum. In backfat, feed restriction increased the polyunsaturated fatty acid proportion and iodine value and lowered the thrombogenic and atherogenic indices. Intramuscular lipid content was reduced by restriction, which did not affect either the fatty acid composition or the oxidative stability in both raw and cooked muscle. Feed restriction improved the nutritional quality of lipids without impairing their technological attributes

Anisakid and Raphidascaridid parasites in Trachurus trachurus: infection drivers and possible effects on the host’s condition

This study investigated the distribution of nematode larvae of Anisakidae and Raphidascarididae (genera Anisakis and Hysterothylacium) in Trachurus trachurus (Linnaeus, 1758) in the Ligurian and central-northern Tyrrhenian Seas. The relationship between the number of parasites and the length and weight parameters of the fish was assessed, and the possible effect of the parasites on the condition factor was evaluated. A total of 190 T. trachurus specimens were collected in July 2019. Parasites were found in 70 individuals. A total of 161 visible larvae were collected in the viscera. Morphological analysis revealed the presence of Anisakis spp. in 55 fish and Hysterothylacium spp. in 15 fish, while 5 fish showed coinfection with both genera. The specimens subjected to PCR (n = 67) showed that 85% of the Anisakis larvae analyzed belonged to the species A. pegreffii, while the remaining 15% belonged to hybrids of A. pegreffii-A. simplex (s.s.). A total of 58% (n = 7) of the Hysterothylacium larvae analyzed belonged to the species H. fabri, while 42% belonged to the species H. aduncum. Our results support the hypothesis that infection with these parasites does not affect the condition of the fish host analyzed, and that body size and depth are major drivers in determining infection levels with Anisakid and Raphidascaridid nematodes

Effect of feeding maltodextrins and dextrose on rearing and slaughtering performance of immunocastrated male pigs.

This study aimed to verify previous research findings showing that feeding maltodextrins (M) and dextrose (D) leads to a reduction of voluntary feed intake in the Italian heavy pig. This may be interesting for immunocastrated pigs fed ad libitum (ad lib.), whose feed intake skyrockets after the 2nd vaccination, causing too fatty carcasses at slaughtering. Thirty-six male pigs (Italian Duroc x Italian Large White crossbred) received a double immunocastrating injection at 90 and 162 days of age. At 120 days, weighing 51.84+4.38 kg, the subjects were evenly housed in 9 boxes, fed ad lib. till the 2nd injection and then given, until slaughtering (197 days of age; 144.51±9.70 kg), one of the experimental diets: control diet, ad lib. (CL); control diet, restricted at 7.5% l.w.0.75 (CR); with MD (3.5 + 3.5%; adjusted for energy and protein), ad lib. (MD). The CR diet was introduced as the alternative choice to avoid too fatty carcasses. Compared to the restricted ones, pigs fed ad lib. (with or without MD) showed statistically higher (P<.01) ADG (1325 and 1325 vs 905 g/d), ADFI (4630 and 4637 vs 2760 g/d), feed:gain ratio (3.53 and 3.52 vs 3.11), and heavier carcasses (125.1 and 124.4 vs 113.2 kg; P<.01) with lower lean meat content (52.21 and 52.09 vs 55.83 %; P<.01). The results point out how immunocastrated pigs fed ad lib. showed similar rearing and slaughtering performance regardless of the inclusion of M and D in the diet

A peculiar Spanish Timber Floor, the "Revoltón": a Diagnostic Example at the "Palacio del Marqués de Benicarló"

The paper deals with the application of the Italian method for assessing on-site and diagnosing timber elements to a distinctive Spanish type of horizontal structure called "revolton" i.e. jack arch floor. As in Spain does not exist a specific regulation for the on-site diagnosis of timber elements, this analysis was performed according to the Italian standard UNI 11119. Due to the peculiar features of the jack arch floor some modifications were applied to the method. During spring 2012 the authors had the opportunity to carry out a pilot project by making a diagnostic analysis on a whole three stories building. The building, Marquees of Benicarlo's Palace, located in Spain in the town of Benicarlo, between Barcelona and Valencia, was erected during the second half of the XVIII century. It maintains the original internal distribution and structure, and it is characterized by precious ceramic decorations on walls and floors. All the horizontal structures studied inside the palace were jack arch floor except the roof structure that was also made of timber. The results of the project described in the paper clarify the specific problems of the horizontal structures and the roof surveyed during the fieldwork. These results are accompanied by several plans that represent the information recovered on-site and lead to a complete assessment of the structures. The plans include the grading based on wood defects, the local moisture content of wood, the superficial and inner decay due to insects and rot and the deformation of the joists. In this way it was possible to locate the areas of the palace where more problems were concentrated, and better understand the causes and origin of the damages.Diodato, M.; Macchioni, N.; Brunetti, M.; Pizzo, B.; Nocetti, M.; Burato, P.; Sozzi, L.... (2013). A peculiar Spanish Timber Floor, the "Revoltón": a Diagnostic Example at the "Palacio del Marqués de Benicarló". Advanced Materials Research. 778:1064-1071. doi:10.4028/www.scientific.net/AMR.778.1064S10641071778E. Cubero, El lujo señorial de lo sencillo (The noble luxury of simplicity), Revistart: Revista de las artes, 125 (2007) pp.8-10.C. Colón de Carvajal y Fibla, La casa-palacio de los Marqueses de Benicarló (The Marquees of Benicarló's Palace), Numen. Revista de excelencia, (2011) on-line.La casa de los Marqueses de Benicarló nos abre sus puertas (The Marquees of Benicarló's House opens its doors), MasQrural, 21 (2008) pp.85-94.M. Diodato, Variaciones constructivas y formales en forjados y cubiertas (Constructive and formal variations in floors and roofs), in: F. Vegas, C. Mileto, Valencia. Centro histórico. Ocho siglos de arquitectura residencial (Historic centre. Eight Centuries of residential architecture), Generalitat Valenciana, Valencia, (2013).N. Macchioni, Inspection techniques for ancient wooden structures, in: P. Roca, J.L. Gonzalez, E. Onate, P.B. Lourenco (Eds. ), Structural analysis of historical constructions II: possibilities of numerical and experimental techniques, CIMNE, Barcelona, 1998, pp.149-162.N. Macchioni, M. Piazza, Italian standardization activity in the field of diagnosis and restoration of ancient timber structures, in: F.M. Mazzolani (Ed. ), Protection of historical buildings - PROHITECH 09, vol. 1-2, Taylor & Francis Group, London, 2009, pp.349-353.G. Lear, B. Kasal, R. Anthony, Resistance drilling, in: B. Kasal, T. Tannert (Eds. ), In situ assessment of structural timber, State of the art report of the RILEM TC 215-AST, Springer.N. Macchioni, M. Brunetti, B. Pizzo, P. Burato, M. Nocetti, S. Palanti, The timber structures in the Church of the Nativity in Bethlehem: Typologies and diagnosis, Journal of cultural heritage, Vol. 13 Issue 4 Supplement S (2012) pp. E42-E53

Understanding Spanish Timber Jack Arch Floors: Examples of Assessment and Conservation Issues

[EN] Jack arch floors are structures made of timber joists, with round or squared section, and segmental vaults either made of flat tiles or poured plaster conglomerate. They are especially diffused along the Mediterranean coast of Spain and can be considered as a main feature of the historical and vernacular architecture of the area. In order to assess and grade these structures several Spanish standards have been considered; however, even if the information contained is useful the Italian standard for the on-site diagnosis UNI 11119 of 2004 has been preferred. The method described in this standard leads the assessment through the grading of each and every timber element and the assignation of bearing properties that are essential for the consolidation project. This article will expose three examples of jack arch floors of the Valencian Community graded using the Italian standard and the conservation issues that arise from the results.Diodato, M.; Macchioni, N.; Brunetti, M.; Pizzo, B.; Nocetti, M.; Burato, P.; Sozzi, L.... (2015). Understanding Spanish Timber Jack Arch Floors: Examples of Assessment and Conservation Issues. International Journal of Architectural Heritage. 9(6):641-654. https://doi.org/10.1080/15583058.2015.104119364165496Diodato, M., Macchioni, N., Brunetti, M., Pizzo, B., Nocetti, M., Burato, P., … Mileto, C. (2013). A Peculiar Spanish Timber Floor, the «Revoltón»: A Diagnostic Example at the «Palacio del Marqués de Benicarló». Advanced Materials Research, 778, 1064-1071. doi:10.4028/www.scientific.net/amr.778.1064Lear, G., Kasal, B., & Anthony, R. (2010). Resistance Drilling. RILEM State of the Art Reports, 51-57. doi:10.1007/978-94-007-0560-9_5Macchioni, N., Brunetti, M., Pizzo, B., Burato, P., Nocetti, M., & Palanti, S. (2012). The timber structures in the Church of the Nativity in Bethlehem: Typologies and diagnosis. Journal of Cultural Heritage, 13(4), e42-e53. doi:10.1016/j.culher.2012.10.004Riggio, M., Anthony, R. W., Augelli, F., Kasal, B., Lechner, T., Muller, W., & Tannert, T. (2013). In situ assessment of structural timber using non-destructive techniques. Materials and Structures, 47(5), 749-766. doi:10.1617/s11527-013-0093-6Tannert, T., Anthony, R. W., Kasal, B., Kloiber, M., Piazza, M., Riggio, M., … Yamaguchi, N. (2013). In situ assessment of structural timber using semi-destructive techniques. Materials and Structures, 47(5), 767-785. doi:10.1617/s11527-013-0094-5Vegas, F. (2013). Parameterisation of Gypsum Mortar for Alternative Structural Consolidation of Traditional Floors. Advances in Materials, 2(4), 48. doi:10.11648/j.am.20130204.1

Complex interplay between neutral and adaptive evolution shaped differential genomic background and disease susceptibility along the Italian peninsula

The Italian peninsula has long represented a natural hub for human migrations across the Mediterranean area, being involved in several prehistoric and historical population movements. Coupled with a patchy environmental landscape entailing different ecological/cultural selective pressures, this might have produced peculiar patterns of population structure and local adaptations responsible for heterogeneous genomic background of present-day Italians. To disentangle this complex scenario, genome-wide data from 780 Italian individuals were generated and set into the context of European/Mediterranean genomic diversity by comparison with genotypes from 50 populations. To maximize possibility of pinpointing functional genomic regions that have played adaptive roles during Italian natural history, our survey included also ∼250,000 exomic markers and ∼20,000 coding/regulatory variants with well-established clinical relevance. This enabled fine-grained dissection of Italian population structure through the identification of clusters of genetically homogeneous provinces and of genomic regions underlying their local adaptations. Description of such patterns disclosed crucial implications for understanding differential susceptibility to some inflammatory/autoimmune disorders, coronary artery disease and type 2 diabetes of diverse Italian subpopulations, suggesting the evolutionary causes that made some of them particularly exposed to the metabolic and immune challenges imposed by dietary and lifestyle shifts that involved western societies in the last centuries

Triphenylene based metal-pyridine cages

C3-symmetric pyridine containing tris-benzyl-O-substituted hexahydroxytriphenylene derivatives were prepared and used in combination with square-planar Pd(II) and Pt(II) complexes for the self-assembly of molecular cages in solution. The formation of a trigonal bipyramid M3L2 cage was demonstrated by multinuclear NMR analyses and pseudo 2D DOSY experiments and supported by semi-empirical calculation

La saprolegniosi in trote di allevamento: trattamento sperimentale con sali minerali disciolti in vasca di medicazione

RIASSUNTO Il Cloruro di sodio (NaCl) a diversi tempi di trattamento ed a concentrazioni variabili è utilizzato nella terapia di infezioni cutanee in genere e di Saprolegniosi in particolare, in pesci di acqua dolce. Nella nostra sperimentazione, nella prima fase sono stati utilizzati NaCl e CASO (NaCl 67% e CaCl2 33%) a diverse concentrazioni su trote adulte sane in vasche di stabulario allo scopo di rilevare, comparativamente, tollerabilità, assenza di tossicità e durata dei trattamenti; nella seconda è stato utilizzato CASO alla concentrazione di 1,5%, in apposita vasca di medicazione su riproduttori di trota fario (Salmo trutta) ed iridea (Salmo gairdnerii) colpite da Saprolegniosi. Le trote, dopo sette giorni di permanenza in vasca di medicazione sono state catturate e sottoposte a controlli clinici, esami micologici e batteriologici. Le lesioni cutanee hanno evidenziato il distacco delle pseudomembrane, la progressiva riduzione delle aree ulcerose e l’inizio del processo di cicatrizzazione. Gli esami micologici e batteriologici hanno evidenziato l’assenza di miceti e batteri sulle ulcere cutanee. I riproduttori, successivamente tenuti in osservazione per tre settimane in vasca post medicazione, hanno mostrato riadattamento all’acqua dolce, assenza di sintomi generali ed ulteriore progresso dei processi riparativi cutanei. SUMMARY In the therapy of skin infection, and in particular in the therapy of Saprolegniosis in the freshwater fish, the Sodium Chloride is utilized at various times of treatment and at variable concentrations. In our study, in a first step, we have used NaCl and CASO (NaCl 67% and CaCl2 33%) on healthy mature trout at various concentrations, to estab- lish tolerability, toxicity and times of treatment. In a second step we have used in a medicated fish pond, on mature trout with saprolegniosis, CASO at the concentration of 1.5%. Clinical controls were done at six, twelve and twenty-four hours, and at 2, 3, 4, 5, 6 days. After 7 days of treatment some trout were captured for clinical, mycological and bacteriological controls. In the skin lesions, we saw the detaching of the pseudomembranes, the progressive reduction of ulcerous areas and the start of the healing process. Mycological and bacteriological exams have shown the absence of fungus and bacteria on skin ulcer. Then, the trout were transferred in a no medicated fish pond and observed for other tree week. In this period we looked at the readaptation at the freshwater, the absence of general symptoms and the progress of healing process of the skin

Culture, modelli organizzativi e traiettorie evolutive osservate nella rete CGM

Nel presente capitolo si analizzano le trasformazioni politiche e sociali che hanno posto l\u2019attenzione dei policy maker sulla territorializzazione del welfare (\ua7 5.1). Il focus viene posto sui problemi e le opportunit\ue0 che la governance societaria e la dimensione operativa locale offrono all\u2019impresa sociale in modo che essa possa valorizzare la propria cittadinanza (\ua7 5.2) generando valore condiviso (\ua7 5.3). Il welfare aziendale inserito in logiche di cluster e di comunit\ue0, pu\uf2 contribuire a sviluppare il territorio attraverso le relazioni virtuose che si vanno a sviluppare tra enti locali, aziende, imprese sociali, cittadini (\ua7 5.4). Il capitolo prosegue con la presentazione dei risultati di un\u2019indagine esplorativa fatta all\u2019interno del gruppo CGM per analizzare i modelli organizzativi e la cultura dei welfare manager (\ua7 5.5). Segue un approfondimento dedicato a tre differenti modelli organizzativi territoriali ritenute rilevanti in termini di programmazione, investimento e innovazione sociale (\ua7 5.6). Il capitolo si chiude con un paragrafo che analizza le prospettive di sviluppo dell\u2019impresa sociale nell\u2019ambito del welfare aziendale territoriale (\ua7 5.7