Preliminary account on the intensity of fouling in Karwar waters

Biology of fouling in Karwar waters is presented. The composition of fouling communities, their fluctuations in relation to hydrographical factors such as temperature, dissolved oxygen, salinity and the influence of the nature and texture of the substratum on fouling communities are discussed

Role of Apamarga Yavakshara in the management of Mutrashmari - A Conceptual Study

Mutrashmari is one of the most common disorders of the Mutravaha Srotas. It is one of the Astamahagada and considered as "Yama” because sometimes it causes acute excruciating pain.[1] In contemporary medical science it is correlated with urolithiasis. Symptoms in general include radiating pain from loin to groin, hematuria, burning micturition, malaise. Prevalence of Urolithiasis varies according to geographical distribution, sex and age. The treatment modalities of urolithiasis in conventional science are conservative medications and surgical procedures which are expensive, involve invasive treatments, needs hospitalization and in most of the cases recurrence rate is high. Ayurveda explains variety of Yogas for the management of Mutrashmari. A combination of Apamarga and Yava Kshara is indicated in Mutrashmari as per Rasatarangini.[2] So this study is taken up, to explore the combined effect of Apamarga and Yava Kshara in Mutrashmari keeping in view the shortcomings of different modern medical treatments

Human Synaptic Plasticity Gene Expression Profile and Dendritic Spine Density Changes in HIV-Infected Human CNS Cells: Role in HIV-Associated Neurocognitive Disorders (HAND)

HIV-associated neurocognitive disorders (HAND) is characterized by development of cognitive, behavioral and motor abnormalities, and occur in approximately 50% of HIV infected individuals. Our current understanding of HAND emanates mainly from HIV-1 subtype B (clade B), which is prevalent in USA and Western countries. However very little information is available on neuropathogenesis of HIV-1 subtype C (clade C) that exists in Sub-Saharan Africa and Asia. Therefore, studies to identify specific neuropathogenic mechanisms associated with HAND are worth pursuing to dissect the mechanisms underlying this modulation and to prevent HAND particularly in clade B infection. In this study, we have investigated 84 key human synaptic plasticity genes differential expression profile in clade B and clade C infected primary human astrocytes by using RT2 Profile PCR Array human Synaptic Plasticity kit. Among these, 31 and 21 synaptic genes were significantly (≥3 fold) down-regulated and 5 genes were significantly (≥3 fold) up-regulated in clade B and clade C infected cells, respectively compared to the uninfected control astrocytes. In flow-cytometry analysis, down-regulation of postsynaptic density and dendrite spine morphology regulatory proteins (ARC, NMDAR1 and GRM1) was confirmed in both clade B and C infected primary human astrocytes and SK-N-MC neuroblastoma cells. Further, spine density and dendrite morphology changes by confocal microscopic analysis indicates significantly decreased spine density, loss of spines and decreased dendrite diameter, total dendrite and spine area in clade B infected SK-N-MC neuroblastoma cells compared to uninfected and clade C infected cells. We have also observed that, in clade B infected astrocytes, induction of apoptosis was significantly higher than in the clade C infected astrocytes. In conclusion, this study suggests that down-regulation of synaptic plasticity genes, decreased dendritic spine density and induction of apoptosis in astrocytes may contribute to the severe neuropathogenesis in clade B infection

Simulation Studies on Arrival Time Distributions of Cherenkov Photons in Extensive Air Showers

Atmospheric Cherenkov technique is an established methodology to study TeV energy gamma rays. Here we carry out systematic monte carlo simulation studies of the timing information of Cherenkov photons. Extensive studies have already been carried out in this regard. Most of these are carried out at higher energies with the aim of studying the elemental composition of cosmic rays. However not much attention is paid to the species dependent signatures at TeV energies. In this work, functional fits have been carried out to the spherical Cherenkov shower fronts and the radii of curvature have been found to be equal to the height of shower maximum irrespective of the species or the observation level. Functional fits have also been carried out to describe the pulse shapes at various core distances in terms of well known probability density distribution functions (PDF). Two types of PDF's have been tried viz. gamma function and lognormal function. The variation of the pulse shape parameters as a function of primary energy, observation height and incident angles have been studied. The possibility of deriving the pulse shape parameters like the rise & decay times, full width at half maximum from the easily measurable quantities like the mean and RMS variation of photon arrival times offers a very important new technique which can be easily applied in an observation.Comment: 34 pages, 14 figures, accepted for publication in Astroparticle Physic

Possible Discrimination between Gamma Rays and Hadrons using Cerenkov Photon Timing Measurements

Atmospheric \v{C}erenkov Technique is an established methodology to study $TeV$ energy gamma rays. However the challenging problem has always been the poor signal to noise ratio due to the presence of abundant cosmic rays. Several ingenious techniques have been employed to alleviate this problem, most of which are centred around the \v{C}erenkov image characteristics. However there are not many techniques available for improving the signal to noise ratio of the data from wavefront sampling observations. One such possible technique is to use the \v{C}erenkov photon arrival times and identify the species dependent characteristics in them. Here we carry out systematic monte carlo simulation studies of the timing information of \v{C}erenkov photons at the observation level. We have parameterized the shape of the \v{C}erenkov shower front as well as the pulse shapes in terms of experimentally measurable quantities. We demonstrate the sensitivity of the curvature of the shower front, pulse shape parameters as well as the photon arrival time jitter to primary species and show their efficiency in improving the signal to noise ratio. The effect of limiting the \v{C}erenkov telescope opening angle by using a circular focal point mask, onthe efficacy of the parameters has also been studied for each of the parameters. Radius of the shower front, pulse decay time and photon arrival time jitter have been found to be the most promising parameters which could be used to discriminate $\gamma -$ray events from the background. We also find that the efficiency of the first two parameters increases with zenith angle and efficiency of pulse decay time decreases with increasing altitude of observation.Comment: 30 pages, 5 postscript figures, uses elsart.sty; To appear in Astroparticle Physic

Positional isomerism markedly affects the growth inhibition of colon cancer cells by NOSH-aspirin: COX inhibition and modeling☆

We recently reported the synthesis of NOSH-aspirin, a novel hybrid that releases both nitric oxide (NO) and hydrogen sulfide (H2S). In NOSH-aspirin, the two moieties that release NO and H2S are covalently linked at the 1, 2 positions of acetyl salicylic acid, i.e. ortho-NOSH-aspirin (o-NOSH-aspirin). In the present study, we compared the effects of the positional isomers of NOSH-ASA (o-NOSH-aspirin, m-NOSH-aspirin and p-NOSH-aspirin) to that of aspirin on growth of HT-29 and HCT 15 colon cancer cells, belonging to the same histological subtype, but with different expression of cyclooxygenase (COX) enzymes; HT-29 express both COX-1 and COX-2, whereas HCT 15 is COX-null. We also analyzed the effect of these compounds on proliferation and apoptosis in HT-29 cells. Since the parent compound aspirin, inhibits both COX-1 and COX-2, we also evaluated the effects of these compounds on COX-1 and COX-2 enzyme activities and also performed modeling of the interactions between the positional isomers of NOSH-aspirin and COX-1 and COX-2 enzymes. We observed that the three positional isomers of NOSH aspirin inhibited the growth of both colon cancer cell lines with IC50s in the nano-molar range. In particular in HT-29 cells the IC50s for growth inhibition were: o-NOSH-ASA, 0.04±0.011 µM; m-NOSH-ASA, 0.24±0.11 µM; p-NOSH-ASA, 0.46±0.17 µM; and in HCT 15 cells the IC50s for o-NOSH-ASA, m-NOSH-ASA, and p-NOSH-ASA were 0.062 ±0.006 µM, 0.092±0.004 µM, and 0.37±0.04 µM, respectively. The IC50 for aspirin in both cell lines was \u3e5 mM at 24 h. The reduction of cell growth appeared to be mediated through inhibition of proliferation, and induction of apoptosis. All 3 positional isomers of NOSH-aspirin preferentially inhibited COX-1 over COX-2. These results suggest that the three positional isomers of NOSH-aspirin have the same biological actions, but that o-NOSH-ASA displayed the strongest anti-neoplastic potential

A rare presentation of the Klinefelter's syndrome

A 16 years old boy with Chronic Renal Failure (CRF) was not suspected of having Klinefelter's syndrome until he complained of painful gynecomastia. He was under haemodialysis for 2 years. At first, he was in an approximately full pubertal development (P5, G5), but he had a small and a firm testis (length 2.2cm) and some degree of facial male pattern hair. He also had a decreased upper to lower body segment ratio and despite having chronic renal failure, he was taller than his parents and siblings. His laboratory tests showed high levels of FSH and normal levels of LH and testosterone. With regards to all these findings, we suspected that there might be an occult Klinefelter's syndrome. So, we made his karyotype that showed a 47XXY pattern. Because there are only a few number of cases that have occult Klinefelter's syndrome in the basis of chronic renal failure, we decided to report this case

Lathyrus genetic resources network: Proceedings of a IPGRI-ICARDA-ICAR Regional Working Group Meeting, 8-10 December 1997, New Delhi, India

Neglected and underutilized crops ”have received relatively less attention in national or international priorities. IPGRI has recognized the potential importance of such crops, and in partnership with others around the world, has undertaken a series of activities to understand and promote the role of such crops in helping to achieve food security and agricultural sustainability through local peoples” maintenance of diversity and in realizing their potential for improvement. The potential of Lathyrus sativus (Grass pea) as a nutritious pulse with high quality grain and fodder, is well recognized in South Asia, Ethiopia and parts of Central, West and North Asia, where other species of this genepool also occur. The local types are tolerant to flood and drought conditions and possess unique adaptation as a post-rice crop across much of South Asia. Other cultivated species of Lathyrus are L. ochrus and L. cicera mainly grown in Central, West and North Africa as a fodder crop. These proceedings are the outcome of the first meeting of this Working Group. This meeting, which took place at NBPGR, New Delhi in December 1997, reviewed progress, reaffirmed the concern and interest of the participating countries in this crop and worked out on the modalities of the Lathyrus Genetic Resources Network (LGRN) - its proposed structure and functioning

Cerenkov Photon Density Fluctuations in Extensive Air Showers

The details of Cerenkov light produced by a gamma ray or a cosmic ray incident at the top of the atmosphere is best studied through systematic simulations of the extensive air showers. Recently such studies have become all the more important in view of the various techniques resulting from such studies, to distinguish gamma ray initiated showers from those generated by much more abundant hadronic component of cosmic rays. We have carried out here such systematic simulation studies using CORSIKA package in order to understand the Cerenkov photon density fluctuations for 5 different energies at various core distances both for gamma ray and proton primaries incident vertically at the top of the atmosphere. Such a systematic comparison of shower to shower density fluctuations for gamma ray and proton primaries is carried out for the first time here. It is found that the density fluctuations are significantly non-Poissonian. Such fluctuations are much more pronounced in the proton primaries than gamma ray primaries at all energies. The processes that contribute significantly to the observed fluctuations have been identified. It has been found that significant contribution to fluctuations comes from photons emitted after shower maximum. The electron number fluctuations and correlated emission of Cerenkov photons are mainly responsible for the observed fluctuations.Comment: 31 pages, latex, 16 figures in ps files, Accepted for publication in "Astroparticle Physics