A Godel-Friedman cosmology?

Based on the mathematical similarity between the Friedman open metric and Godel's metric in the case of nearby distances, we investigate a new scenario for the Universe's evolution, where the present Friedman universe originates from a primordial Godel universe by a phase transition during which the cosmological constant vanishes. Using Hubble's constant and the present matter density as input, we show that the radius and density of the primordial Godel universe are close, in order of magnitude, to the present values, and that the time of expansion coincides with the age of the Universe in the standard Friedman model. In addition, the conservation of angular momentum provides, in this context, a possible origin for the rotation of galaxies, leading to a relation between the masses and spins corroborated by observational data.Comment: Extended version, accepted for publication in Physical Review

Zebrafish as outgroup model to study evolution of scavenger receptor class B type I functions

Background and aims: Scavenger receptor class B1 (SCARB1) -also known as the high-density lipoprotein (HDL) receptor -is a multi-ligand scavenger receptor that is primarily expressed in liver and steroidogenic organs. This receptor is known for its function in reverse cholesterol transport (RCT) in mammals and hence disruption leads to a massive increase in HDL cholesterol in these species. The extracellular domain of SCARB1 -which is important for cholesterol handling -is highly conserved across multiple vertebrates, except in zebrafish. Methods: To examine the functional conservation of SCARB1 among vertebrates, two stable scarb1 knockout zebrafish lines, scarb1 715delA (scarb1-1 nt) and scarb1 715_716insGG (scarb1 +2 nt), were created using CRISPR-Cas9 technology. Results: We demonstrate that, in zebrafish, SCARB1 deficiency leads to disruption of carotenoid-based pigmen-tation, reduced fertility, and a decreased larvae survival rate, whereas steroidogenesis was unaltered. The observed reduced fertility is driven by defects in female fertility (-50 %, p Drug Delivery Technolog

A mathematical analysis of the evolution of perturbations in a modified Chaplygin gas model

One approach in modern cosmology consists in supposing that dark matter and dark energy are different manifestations of a single `quartessential' fluid. Following such idea, this work presents a study of the evolution of perturbations of density in a flat cosmological model with a modified Chaplygin gas acting as a single component. Our goal is to obtain properties of the model which can be used to distinguish it from another cosmological models which have the same solutions for the general evolution of the scale factor of the universe, without the construction of the power spectrum. Our analytical results, which alone can be used to uniquely characterize the specific model studied in our work, show that the evolution of the density contrast can be seen, at least in one particular case, as composed by a spheroidal wave function. We also present a numerical analysis which clearly indicates as one interesting feature of the model the appearence of peaks in the evolution of the density constrast.Comment: 21 pages, accepted for publication in General Relativity and Gravitatio

Efeitos da radiação gama na conservação de variedades de cebola ( Allium cepa) da região do São Francisco, Pernambuco

Three varieties of onion (Allium cepa), "Amarela das Canárias", "Pera de Pernambuco" and Asgrow, cultivated in the São Francisco River region (Pernambuco), were irradiated at doses of 4, 8 and 12 krad, and were stored in Recife, at ambient temperature. In this preliminary experiment, rotting, loss of weight and sprouting of onions were observed during 120 days. After this period, the mean conservation index, expressed as the percent of the initial number of bulbs, reached, for the former two varieties, values of 92, 94 and 83% corresponding to doses of 12, 8 and 4 krad, respectively; whereas the Asgrow variety exhibited a mean conservation index of 3%. The non irradiated Asgrow samples were at that time wholly spoiled. No sprouting was observed in the irradiated samples and the total loss of weight was more marked in the non-irradiated bulbs. The statistical analysis of experimental data indicated that irradiation conservation effects were significant at the 0,1% level for the "Amarela das Canárias" and "Pera de Pernambuco" varieties, as verified through the F-test. Moreover, the analysis of variance displayed no significance for the interaction effects between doses and varieties, and the loss of weight through dehydration, followed up to 55 days did not show differences at the usual significance levels.Três variedades de cebola, Amarela das Canárias, Pera de Pernambuco e Asgrow, bastante cultivadas na região do São Francisco, foram irradiadas com doses de 4, 8 e 12 krad e estocadas à temperatura ambiente, em Recife, PE, sendo observadas durante um período de 120 dias quanto à ocorrência de podridão, perda de peso e germinação. As percentagens médias de conservação após este período nas duas primeiras variedades foram de 92%, 94% e 83% para as doses de 12, 8 e 4 krad, e de apenas 3% para a variedade Asgrow. As testemunhas apresentaram conservação ao nível máximo de apenas 18%. Nenhuma germinação foi observada nas amostras irradiadas e a perda de peso foi mais acentuada nas não irradiadas. A análise estatística dos dados obtidos evidenciou que a conservação nas variedades Amarela das Canárias e Pera de Pernambuco foi significativa ao nível de 0,1% pelo teste F. A análise da variância não mostrou diferenças significativas entre os efeitos da interação dose versus variedade

A model for preservation of thymocyte-depleted thymus

DiGeorge syndrome is a disorder caused by a microdeletion on the long arm of chromosome 22. Approximately 1% of patients diagnosed with DiGeorge syndrome may have an absence of a functional thymus, which characterizes the complete form of the syndrome. These patients require urgent treatment to reconstitute T cell immunity. Thymus transplantation is a promising investigational procedure for reconstitution of thymic function in infants with congenital athymia. Here, we demonstrate a possible optimization of the preparation of thymus slices for transplantation through prior depletion of thymocytes and leukocyte cell lineages followed by cryopreservation with cryoprotective media (5% dextran FP 40, 5% Me2SO, and 5% FBS) while preserving tissue architecture. Thymus fragments were stored in liquid nitrogen at -196°C for 30 days or one year. The tissue architecture of the fragments was preserved, including the distinction between medullary thymic epithelial cells (TECs), cortical TECs, and Hassall bodies. Moreover, depleted thymus fragments cryopreserved for one year were recolonized by intrathymic injections of 3×106 thymocytes per mL, demonstrating the capability of these fragments to support T cell development. Thus, this technique opens up the possibility of freezing and storing large volumes of thymus tissue for immediate transplantation into patients with DiGeorge syndrome or atypical (Omenn-like) phenotype

Sward structure of marandu palisadegrass subjected to continuous stocking and nitrogen-induced rhythms of growth

Acceleration of the growth rhythm of plants using nitrogen fertiliser alters the rates of physiological processes like growth and senescence and may cause significant changes in sward structure, interfering with plant and animal responses. The objective of this experiment was to evaluate sward structure of marandu palisadegrass (Brachiaria brizantha) maintained at 30 cm under continuous stocking and subjected to contrasting rhythms of growth from January 2007 to April 2008. These were generated using or not nitrogen fertiliser, and comprised four experimental treatments as follows: control (non-fertilised), 150, 300 and 450 kg ha-1 of N. Acceleration of the growth rhythm of plants was associated with increases in leaf and stem bulk density, and resulted in larger LAI and total bulk density on swards subjected to faster (fertilised with 300 and 450 kg ha-1 of N) than those subjected to slower rhythms of growth (non-fertilised or fertilised with 150 kg ha-1 of N). Variations in dead material bulk density were associated with seasonal variations in climatic conditions, and were not influenced by growth rhythms. During autumn/winter and early spring (dry period of the year) swards subjected to faster, relative to those subjected to slower rhythms of growth, had larger proportion of leaves on the top horizons. On the other hand, sward structure did not vary among rhythms of growth at times of the year when there was no limitation in the availability of climatic growth factors (late spring and summer), indicating that when control of the grazing process is efficient, changes in sward structure are basically a function of seasonal variations in climatic growth conditions and phenological state of plants.A aceleração do ritmo de crescimento das plantas por meio da adubação nitrogenada altera a velocidade dos processos fisiológicos, atuando sobre o crescimento e a senescência, podendo refletir-se em alterações importantes sobre a estrutura do dossel. Avaliou-se a estrutura do dossel forrageiro de pastos de capim-marandu (Brachiaria brizantha) mantidos a 30 cm de altura por meio de lotação contínua e submetidos a ritmos de crescimento contrastantes de janeiro de 2007 a abril de 2008. Os distintos ritmos de crescimento foram criados por meio da utilização de adubação nitrogenada, segundo os tratamentos: sem adubação (controle), 150, 300 e 450 kg ha-1 de N. A aceleração do ritmo de crescimento dos pastos resultou em aumentos de densidade volumétrica de folhas e de colmos, refletindo em maior IAF e densidade volumétrica total em pastos submetidos aos ritmos de crescimento mais acelerados (adubados com 300 e 450 kg ha-1 de N) relativamente àqueles submetidos a ritmos de crescimento mais lentos (pastos não adubados e/ou adubados com 150 kg ha-1 de N). Variações em densidade volumétrica do material morto estiveram associadas a flutuações estacionais das condições climáticas, e não foram influenciadas pelos ritmos de crescimento avaliados. Na época da seca (outono/inverno e início de primavera), os pastos submetidos aos ritmos de crescimento mais acelerados apresentaram maior proporção de folhas no horizonte superior do dossel. Por outro lado, a estrutura do dossel não variou entre os ritmos de crescimento nas épocas de maior disponibilidade de fatores de crescimento (final de primavera e verão), indicando que quando o controle do processo de pastejo é feito de maneira eficiente, mudanças em estrutura são função basicamente de variações estacionais em condições climáticas e estádio fenológico das plantas

Sex ratio biases in termites provide evidence for kin selection

Contains fulltext : 142755.pdf (publisher's version ) (Closed access)Molecular testing of tumor samples to guide treatment decisions is of increasing importance. Several drugs have been approved for treatment of molecularly defined subgroups of patients, and the number of agents requiring companion diagnostics for their prescription is expected to rapidly increase. The results of such testing directly influence the management of individual patients, with both false-negative and false-positive results being harmful for patients. In this respect, external quality assurance (EQA) programs are essential to guarantee optimal quality of testing. There are several EQA schemes available in Europe, but they vary in scope, size and execution. During a conference held in early 2012, medical oncologists, pathologists, geneticists, molecular biologists, EQA providers and representatives from pharmaceutical industries developed a guideline to harmonize the standards applied by EQA schemes in molecular pathology. The guideline comprises recommendations on the organization of an EQA scheme, defining the criteria for reference laboratories, requirements for EQA test samples and the number of samples that are needed for an EQA scheme. Furthermore, a scoring system is proposed and consequences of poor performance are formulated. Lastly, the contents of an EQA report, communication of the EQA results, EQA databases and participant manual are given

Building The Sugarcane Genome For Biotechnology And Identifying Evolutionary Trends

Background: Sugarcane is the source of sugar in all tropical and subtropical countries and is becoming increasingly important for bio-based fuels. However, its large (10 Gb), polyploid, complex genome has hindered genome based breeding efforts. Here we release the largest and most diverse set of sugarcane genome sequences to date, as part of an on-going initiative to provide a sugarcane genomic information resource, with the ultimate goal of producing a gold standard genome.Results: Three hundred and seventeen chiefly euchromatic BACs were sequenced. A reference set of one thousand four hundred manually-annotated protein-coding genes was generated. A small RNA collection and a RNA-seq library were used to explore expression patterns and the sRNA landscape. In the sucrose and starch metabolism pathway, 16 non-redundant enzyme-encoding genes were identified. 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