430 research outputs found
VALIDATED SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF CHLORAMPHENICOL IN PURE AND IN ITS DOSAGE FORM
Objective: A simple, economic, selective, precise, and accurate UV-Visible spectrophotometric method for the analysis of Chloramphenicol in bulk drug and pharmaceutical formulations was developed and validated in the present study.
Methods: Based on oxidative coupling reaction with MBTH reagent at PH-4.5 which is extractable at 620 nm. The Beer's law is obeyed in the concentration range 1-6 ml (10-60 µg ml-1).
Results: The RSD was found to be 0.0194% and recovery is 99.73%. The method was completely validated and proven to be rugged. The interferences of the ingredients and recipients were not observed. The repeatability and the performance of the proved method were established by point and internal hypothesis and through recovery studies.
Conclusion: The method was found to be accurate and precise, as indicated by recovery studies close to 100 and % RSD is not more than 2. The summery of validation parameters of proposed UV-Visible method is given
VALIDATED SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF CHLORAMPHENICOL IN PURE AND IN ITS DOSAGE FORM
A simple, précis, rapid sensitive and accurate spectrophotometric methods have been developed for the estimation of Chloramphenicol UV in pure form and its pharmaceutical formulations based on oxidative coupling reaction UV with MBTH reagent at P H-4 which is extractable at 620 nm. Beer's law is obeyed in the concentration range 1-6 ml (10-60 µgml-1). The developed method was applied directly and easily for the analysis of the pharmaceutical formulations. RSD was found to be 0.0194 % and recovery 99.73%. The method was completely validated and proven to be rugged. The interferences of the ingredients and excipients were not observed. The repeatability and the performance of the proved method were established by point and internal hypothesis and through recovery studies.Keywords: Spectrophotometry, Chloramphenicol, MBTH, Oxidative coupling
Controversies in the management of early endometrial carcinoma: an update
Endometrial carcinoma (EC) is the commonest genital tract malignancy in developing countries and is usually confined to the uterus at the time of diagnosis with excellent prognosis and high cure rates. But the management is associated with lot of controversies like in staging, best surgical approach, extent of lymphadenectomy, adjuvant therapy, fertility sparing surgery in young women etc. A thorough surgical staging is important to determine uterine and extrauterine spread and also understanding of the pathophysiology and management strategies to identify women who are at high risk and tailoring the adjuvant treatment if necessary without increasing the morbidity. This evidence based narrative review conducted by searching Medline (1994- 2015) and other online articles from Pubmed, Google scholar. Articles were selected based on their currency and relevance to the discussion they summarize the current literature to provide an approach to best practice management of early endometrial carcinoma
Studies on the proximity effect in Bi-based high-temperature superconductor/manganite heterostructures
The effect of proximity of the magnetism of the Pr-based manganite
(Pr0.6Sr0.4MnO3) on the superconductivity of Bi-based high-temperature
superconductor (Bi1.75Pb0.25Sr2Ca2Cu3O10+d) was studied based on the results
obtained from the magnetotransport and magnetization measurements. Decrease in
the values of the upper critical field (HC2(0)) and an increase in the width of
the superconducting transition (Delta TC) of Bi1.75Pb0.25Sr2Ca2Cu3O10+d were
observed in proximity with the manganite. The combined effect of magnetic
exchange interaction arising from the manganite, the leakage of Cooper-pairs
from the superconductor into the manganite, and the diffusion and transport of
spin-polarized electrons from the manganite into the superconductor were found
to modify the superconducting properties of Bi1.75Pb0.25Sr2Ca2Cu3O10+d. The
stacking sequence of the individual layers in these heterostructures was found
to dictate the ground state properties of the heterostructure. As a consequence
of the proximity effect, the colossal-magnetoresistance (CMR) ratio as high as
~ 99 % observed in the heterostructure makes the thin film heterostructures
promising candidates for potential technological applications.Comment: 29 pages, 14 figure
Case Report: High Prenatal Bisphenol A Exposure and Infant Neonatal Neurobehavior
Context: Most of the U.S. population is exposed to the high-production-volume chemical bisphenol A (BPA), but targetable sources of exposure remain to be determined. Animal studies and one human study suggest that BPA is a neurotoxicant
Maximally localized Wannier functions in LaMnO3 within PBE+U, hybrid functionals, and partially self-consistent GW: an efficient route to construct ab-initio tight-binding parameters for e_g perovskites
Using the newly developed VASP2WANNIER90 interface we have constructed
maximally localized Wannier functions (MLWFs) for the e_g states of the
prototypical Jahn-Teller magnetic perovskite LaMnO3 at different levels of
approximation for the exchange-correlation kernel. These include conventional
density functional theory (DFT) with and without additional on-site Hubbard U
term, hybrid-DFT, and partially self-consistent GW. By suitably mapping the
MLWFs onto an effective e_g tight-binding (TB) Hamiltonian we have computed a
complete set of TB parameters which should serve as guidance for more elaborate
treatments of correlation effects in effective Hamiltonian-based approaches.
The method-dependent changes of the calculated TB parameters and their
interplay with the electron-electron (el-el) interaction term are discussed and
interpreted. We discuss two alternative model parameterizations: one in which
the effects of the el-el interaction are implicitly incorporated in the
otherwise "noninteracting" TB parameters, and a second where we include an
explicit mean-field el-el interaction term in the TB Hamiltonian. Both models
yield a set of tabulated TB parameters which provide the band dispersion in
excellent agreement with the underlying ab initio and MLWF bands.Comment: 30 pages, 7 figure
Detection of loci in the leu region of Rhizobium meliloti chromosome
A multi-marked strain of Rhizobium meliloti was developed by the co-mutation method and employed to contribute to the genetic map of R. meliloti chromosome. Seven loci were placed at 5 sites in theleu region in the orderman-aba, fix, leu-cro-azt, ost-thi
Tracking planar orientations of active MRI needles
Purpose: To determine and track the planar orientation of active interventional devices without using localizing RF microcoils. Materials and Methods: An image-based tracking method that determines a device's orientation using projection images was developed. An automated and a manual detection scheme were implemented. The method was demonstrated in an in vivo mesocaval puncture procedure in swine, which required accurate orientation of an active transvascular needle catheter. Results: The plane of the catheter was determined using two projection images. The scan plane was adjusted automatically to follow the catheter plane, and its orientation with respect to a previously acquired target plane was displayed. The algorithm facilitated navigation for a fast and accurate puncture. Conclusion: Using image-based techniques, with no mechanical design changes, the orientation of an active intravascular probe could be tracked. © 2007 Wiley-Liss, Inc
Evolution of Superconducting Properties of Coexistent Bi-2212 and Bi-2223 phases in BSCCO
391-397The evolution of superconducting properties of BSCCO superconductors, inadvertently hosting the two superconducting phases Bi-2212 and Bi-2223 have been investigated in pristine and Pb doped BSCCO. The superconducting transition temperature TC of Bi-2212 phase monotonically increases with increasing Bi-2223 phase fraction. On the other hand Bi-2223 phase exhibits depression in TC for its lower phase fractions (<24%) but attains its bulk value as Bi-2223 phase fraction is increased to 30%. This behavior has been rationalized by invoking the interplay of proximity effects between the two coexisting phases and establishment of Bi-2223 superconducting percolation path. In addition to aiding the formation of BSCCO with higher Bi-2223 phase fraction, the Pb substitution also leads to an enhancement of critical current density by the creation of pinning centres
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