Calcium Triggered Lα-H2 Phase Transition Monitored by Combined Rapid Mixing and Time-Resolved Synchrotron SAXS

BACKGROUND: Awad et al. reported on the Ca(2+)-induced transitions of dioleoyl-phosphatidylglycerol (DOPG)/monoolein (MO) vesicles to bicontinuous cubic phases at equilibrium conditions. In the present study, the combination of rapid mixing and time-resolved synchrotron small-angle X-ray scattering (SAXS) was applied for the in-situ investigations of fast structural transitions of diluted DOPG/MO vesicles into well-ordered nanostructures by the addition of low concentrated Ca(2+) solutions. METHODOLOGY/PRINCIPAL FINDINGS: Under static conditions and the in absence of the divalent cations, the DOPG/MO system forms large vesicles composed of weakly correlated bilayers with a d-spacing of approximately 140 A (L(alpha)-phase). The utilization of a stopped-flow apparatus allowed mixing these DOPG/MO vesicles with a solution of Ca(2+) ions within 10 milliseconds (ms). In such a way the dynamics of negatively charged PG to divalent cation interactions, and the kinetics of the induced structural transitions were studied. Ca(2+) ions have a very strong impact on the lipidic nanostructures. Intriguingly, already at low salt concentrations (DOPG/Ca(2+)>2), Ca(2+) ions trigger the transformation from bilayers to monolayer nanotubes (inverted hexagonal phase, H(2)). Our results reveal that a binding ratio of 1 Ca(2+) per 8 DOPG is sufficient for the formation of the H(2) phase. At 50 degrees C a direct transition from the vesicles to the H(2) phase was observed, whereas at ambient temperature (20 degrees C) a short lived intermediate phase (possibly the cubic Pn3m phase) coexisting with the H(2) phase was detected. CONCLUSIONS/SIGNIFICANCE: The strong binding of the divalent cations to the negatively charged DOPG molecules enhances the negative spontaneous curvature of the monolayers and causes a rapid collapsing of the vesicles. The rapid loss of the bilayer stability and the reorganization of the lipid molecules within ms support the argument that the transition mechanism is based on a leaky fusion of the vesicles

Rapport IV-7 Approfondissement du sol et économie de l’eau

Deep ploughing of plots destined for perennial or even annual crops increases root density in the freshly accessible deeper layers. This increases the water capacity of the soil and makes the water immediately available. The effect of deepening the soil on water economy is discussed for irrigated and non-irrigated crops in relation to crop type, method of water distribution, and climate.Le travail profond du sol avant mise en place de cultures pérennes, ou même de cultures annuelles, a pour effet la densification du niveau des racines dans les tranches rendues pénétrables. Ainsi sont augmentées à la fois la capacité du sol pour l’eau et la disponibilité instantanée de cette eau. On examine les conséquences de l’approfondissement du sol sur l’économie de l’eau en culture sèche ou irriguée en fonction de la nature de la culture, du mode de distribution de l’eau, des conditions climatiques.Panine M., Rutten P. Rapport IV-7 Approfondissement du sol et économie de l’eau. In: L'hydrotechnique au service d'une politique de l'eau. Evolution des problèmes de l'eau au cours de la dernière décennie. Compte rendu des XVes journées de l'hydraulique. Toulouse, 5, 6 et 7 septembre 1976. Tome 2, 1979

Structural observation and kinetic pathway in the formation of polymeric micelles

The route by which amphiphilic molecules self-assemble into micelles is still not fully understood. In this Letter, we present direct structural information on the birth and growth of block copolymer micelles by means of synchrotron x-ray scattering with millisecond time resolution. Using a quantitative model, we show that the self-assembly process can be viewed as a nucleation and growth type process where the elemental growth mechanism is an exchange of single molecules

Combined SAXS−Rheological Studies of Liquid-Crystalline Colloidal Dispersions of Mineral Particles

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Shear-induced smectic order in isotactic polypropylene revisited

We provide evidence that earlier reported smectic ordering in the melt of isotactic polypropylene is not intrinsic to the system but results from the presence of small quantities of calcium stearate. No causal relation exists between this effect and the crystn. properties of iPP

Wormlike micelle formation and flow alignment of a pluronic block copolymer in aqueous solution

The self-assembly into wormlike micelles of a poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) triblock copolymer Pluronic P84 in aqueous salt solution (2 M NaCl) has been studied by rheology, small-angle X-ray and neutron scattering (SAXS/SANS), and light scattering. Measurements of the flow curves by controlled stress rheometry indicated phase separation under flow. SAXS on solutions subjected to capillary flow showed alignment of micelles at intermediate shear rates, although loss of alignment was observed for high shear rates. For dilute solutions, SAXS and static light scattering data on unaligned samples could be superposed over three decades in scattering vector, providing unique information on the wormlike micelle structure over several length scales. SANS data provided information on even shorter length scales, in particular, concerning "blob" scattering from the micelle corona. The data could be modeled based on a system of semiflexible self-avoiding cylinders with a circular cross-section, as described by the wormlike chain model with excluded volume interactions. The micelle structure was compared at two temperatures close to the cloud point (47 degrees C). The micellar radius was found not to vary with temperature in this region, although the contour length increased with increasing temperature, whereas the Kuhn length decreased. These variations result in an increase of the low-concentration radius of gyration with increasing temperature. This was consistent with dynamic light scattering results, and, applying theoretical results from the literature, this is in agreement with an increase in endcap energy due to changes in hydration of the poly(ethylene oxide) blocks as the temperature is increased

The measurement by SAXS of the nematic order parameter of laponite gels

We performed small-angle X-ray scattering (SAXS) experiments on oriented samples of laponite clay gels obtained by slow evaporation. The SAXS patterns are clearly anisotropic, which demonstrates the existence of nematic-like orientational correlations of the laponite disc-like particles. The iso-intensity lines of the SAXS patterns are elliptical and roughly homothetic over the whole scattering vector range examined in this experiment. The value of the nematic order parameter, , derived from the SAXS patterns is comparable to that of usual liquid crystals. This large value proves the importance of orientational correlations in these gels at concentrations higher than 0.02\un{g\cdot cm^{-3}}, even in the absence of shear