Evaluation of antioxidant potential, enzyme inhibition activity and phenolic profile of Lathyrus cicera and Lathyrus digitatus: Potential sources of bioactive compounds for the food industry

The genus Lathyrus has great importance in terms of food and agricultural areas. In this study, the in vitro antioxidant activity (phosphomolybdenum, DPPH, ABTS, FRAP, CUPRAC and metal chelating) and enzyme inhibitory activity evaluation (acetylcholinesterase, butyrylcholinesterase, alpha-amylase and alpha-glucosidase) of L. cicera and L. digitatus were investigated, as, well as their phytochemical profiles. The screening of the main phytochemical compounds in aerial parts of L. cicera and L. digitatus was carried out by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn), observing that flavonoids represent the highest percentage of identified compounds, with abundance of tri- and tetra-glycosilated flavonoids, including acylated ones, especially in L. cicera. Generally, L. digitatus exhibited stronger antioxidant and enzyme inhibitory activities in correlation with its higher level of phenolics. The high number of phenolic compounds and the results of the antioxidant and enzyme assays suggest that these plants may be further used as sources of bioactive compounds, and for the preparation of new nutraceuticals. (C) 2017 Elsevier Ltd. All rights reserved

Lathyrus aureus and Lathyrus pratensis: characterization of phytochemical profiles by liquid chromatography-mass spectrometry, and evaluation of their enzyme inhibitory and antioxidant activities

Different wild plants commonly used in folk medicine, such as different species from the genus Lathyrus, may represent new sources of biologically active compounds. Hence, a study of the composition and (bio)chemical behaviour of extracts from these plants may provide valuable information. To evaluate the phytochemical profile, and the enzyme inhibition and antioxidant activities of the aerial parts of L. pratensis and L. aureus, extracts from both plants were analyzed by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn). The in vitro antioxidant activity (phosphomolybdenum, beta-carotene bleaching, DPPH, ABTS, FRAP, CUPRAC and metal chelating) and enzyme inhibitory activity (acetyl cholinesterase, butyrylcholinesterase, tyrosinase, alpha-amylase and alpha-glucosidase) were also investigated for these Lathyrus species. Flavonoids and saponins were the main groups of compounds detected in the extracts from both plants. Generally, the methanol and water extracts presented remarkable antioxidant and enzyme inhibitory effects; all the observed results are critically discussed. The content of organic compounds and the antioxidant and enzyme assays suggest that these plants may be further used in phytopharmaceutical or food industry applications