Perancangan Logo Bakso Perdana Dan Media Pendukung

Bakso Perdana adalah bakso yang menawarkan varian bakso. Varian bakso yang paling digemari adalah bakso manisnya. Bakso Perdana juga memiliki varian bakso yaitu Bakso Puyuh, Bakso Ati, Bakso Kikil, Bakso Urat. Namun bakso manislah yang sangat digemari oleh para konsumen. Namun sayangnya Bakso Perdana ini belum memiliki sebuah identitas yang menjadi suatu identitas USAha, oleh karena itu pihak Bakso Perdana ini meminta untuk mendesain corporate identity yang sesuai dengan citra Perusahaan melalui Perancangan Corporate Identity Bakso Perdana dan media pendukun

Perancangan Kemasan Madu Buen Kalimantan Timur Beserta Media Pendukungnya

Indonesia merupakan negara yang memiliki potensi dalam menghasilkan madu. Banyaknya madu yang beredar di pasaran menyebabkan persaingan yang cukup tinggi, sehingga nilai jual madu menjadi rendah. Madu Buen adalah salah satu merek madu organik hutan liar di Indonesia yang diambil dari hutan di Kalimantan Timur. Bersaing dengan merek madu lainnya, Madu Buen ingin membedakan dirinya dengan madu lain melalui kemasan. Hal ini juga dikarenakan kemasan kurang melindungi madu di dalamnya. Penelitian ini mempelajari dan merancang suatu kemasan madu yang dapat membedakan Madu Buen dengan madu lainnya, serta merancang kemasan yang baik yang dapat melindungi madu di dalamnya

Long Range Anticorrelations and Non-Gaussian Behavior of a Leaky Faucet

We find that intervals between successive drops from a leaky faucet display scale-invariant, long-range anticorrelations characterized by the same exponents of heart beat-to-beat intervals of healthy subjects. This behavior is also confirmed by numerical simulations on lattice and it is faucet-width- and flow-rate-independent. The histogram for the drop intervals is also well described by a L\'evy distribution with the same index for both histograms of healthy and diseased subjects. This additional result corroborates the evidence for similarities between leaky faucets and healthy hearts underlying dynamics.Comment: Self-extracting uuencoded postscript file. Phys.Rev.E (Rap.Comm.). Related papers can be found at http://www.if.uff.br/~tjpp/tjppe.htm

Partial duplication of the PRLR and SPEF2 genes at the late feathering locus in chicken

Background One of the loci responsible for feather development in chickens is K. The K allele is partially dominant to the k+ allele and causes a retard in the emergence of flight feathers at hatch. The K locus is sex linked and located on the Z chromosome. Therefore, the locus can be utilized to produce phenotypes that identify the sexes of chicks at hatch. Previous studies on the organization of the K allele concluded the integration of endogenous retrovirus 21 (ev21) into one of two large homologous segments located on the Z chromosome of late feathering chickens. In this study, a detailed molecular analysis of the K locus and a DNA test to distinguish between homozygous and heterozygous late feathering males are presented. Results The K locus was investigated with quantitative PCR by examining copy number variations in a total of fourteen markers surrounding the ev21 integration site. The results showed a duplication at the K allele and sequence analysis of the breakpoint junction indicated a tandem duplication of 176,324 basepairs. The tandem duplication of this region results in the partial duplication of two genes; the prolactin receptor and the gene encoding sperm flagellar protein 2. Sequence analysis revealed that the duplication is similar in Broiler and White Leghorn. In addition, twelve late feathering animals, including Broiler, White Leghorn, and Brown Layer lines, contained a 78 bp breakpoint junction fragment, indicating that the duplication is similar in all breeds. The breakpoint junction was used to develop a TaqMan-based quantitative PCR test to allow distinction between homozygous and heterozygous late feathering males. In total, 85.3% of the animals tested were correctly assigned, 14.7% were unassigned and no animals were incorrectly assigned. Conclusion The detailed molecular analysis presented in this study revealed the presence of a tandem duplication in the K allele. The duplication resulted in the partial duplication of two genes; the prolactin receptor and the gene encoding sperm flagellar protein 2. Furthermore, a DNA test was developed to distinguish between homozygous and heterozygous late feathering males

Deleterious Mutations in the TPO Gene Associated with Familial Thyroid Follicular Cell Carcinoma in Dutch German Longhaired Pointers

Familial thyroid cancer originating from follicular cells accounts for 5–15% of all the thyroid carcinoma cases in humans. Previously, we described thyroid follicular cell carcinomas in a large number of the Dutch German longhaired pointers (GLPs) with a likely autosomal recessive inheritance pattern. Here, we investigated the genetic causes of the disease using a combined approach of genome-wide association study and runs of homozygosity (ROH) analysis based on 170k SNP array genotype data and whole-genome sequences. A region 0–5 Mb on chromosome 17 was identified to be associated with the disease. Whole-genome sequencing revealed many mutations fitting the recessive inheritance pattern in this region including two deleterious mutations in the TPO gene, chr17:800788G>A (686F>V) and chr17:805276C>T (845T>M). These two SNP were subsequently genotyped in 186 GLPs (59 affected and 127 unaffected) and confirmed to be highly associated with the disease. The recessive genotypes had higher relative risks of 16.94 and 16.64 compared to homozygous genotypes for the reference alleles, respectively. This study provides novel insight into the genetic causes leading to the familial thyroid follicular cell carcinoma, and we were able to develop a genetic test to screen susceptible dogs.</p

Mining for single nucleotide polymorphisms in pig genome sequence data

<p>Abstract</p> <p>Background</p> <p>Single nucleotide polymorphisms (SNPs) are ideal genetic markers due to their high abundance and the highly automated way in which SNPs are detected and SNP assays are performed. The number of SNPs identified in the pig thus far is still limited.</p> <p>Results</p> <p>A total of 4.8 million whole genome shotgun sequences obtained from the NCBI trace-repository with center name "SDJVP", and project name "Sino-Danish Pig Genome Project" were analysed for the presence of SNPs. Available BAC and BAC-end sequences and their naming and mapping information, all obtained from SangerInstitute FTP site, served as a rough assembly of a reference genome. In 1.2 Gb of pig genome sequence, we identified 98,151 SNPs in which one of the sequences in the alignment represented the polymorphism and 6,374 SNPs in which two sequences represent an identical polymorphism. To benchmark the SNP identification method, 163 SNPs, in which the polymorphism was represented twice in the sequence alignment, were selected and tested on a panel of three purebred boar lines and wild boar. Of these 163 in silico identified SNPs, 134 were shown to be polymorphic in our animal panel.</p> <p>Conclusion</p> <p>This SNP identification method, which mines for SNPs in publicly available porcine shotgun sequences repositories, provides thousands of high quality SNPs. Benchmarking in an animal panel showed that more than 80% of the predicted SNPs represented true genetic variation.</p

Conservation genomic analysis of domestic and wild pig populations from the Iberian Peninsula

Abstract Background Inbreeding is among the major concerns in management of local livestock populations. The effective population size of these populations tends to be small, which enhances the risk of fitness reduction and extinction. High-density SNP data make it possible to undertake novel approaches in conservation genetics of endangered breeds and wild populations.A total of 97 representative samples of domestic and wild pig populations from the Iberian Peninsula, subjected to different levels of threat with extinction, were genotyped with a 60 K SNP panel. Data analyses based on: (i) allele frequency differences; (ii) linkage disequilibrium and (iii) runs of homozygosity were integrated to study population relationships, inbreeding and demographic history. Results The domestic pigs analyzed belonged to local Spanish and Portuguese breeds: Iberian ─ including the variants Retinto Iberian, Negro Iberian and Manchado de Jabugo ─, Bisaro and Chato Murciano. The population structure and persistence of phase analysis suggested high genetic relations between Iberian variants, with recent crossbreeding of Manchado de Jabugo with other pig populations. Chato Murciano showed a high frequency of long runs of homozygosity indicating recent inbreeding and reflecting the recent bottleneck reported by historical records. The Chato Murciano and the Manchado de Jabugo breeds presented the lowest effective population sizes in accordance with their status of highly inbred breeds. The Iberian wild boar presented a high frequency of short runs of homozygosity indicating past small population size but no signs of recent inbreeding. The Iberian breed showed higher genetic similarities with Iberian wild boar than the other domestic breeds. Conclusions High-density SNP data provided a consistent overview of population structure, demographic history and inbreeding of minority breeds and wild pig populations from the Iberian Peninsula. Despite the very different background of the populations used, we found a good agreement between the different analyses. Our results are also in agreement with historical reports and provide insight in the events that shaped the current genetic variation of pig populations from the Iberian Peninsula. The results exposed will aid to design and implement strategies for the future management of endangered minority pig breeds and wild populations.This project was financially supported by European Research Council under the European Community's Seventh Framework Programme (FP7/2007-2013)/ERC grant #ERC-2009-AdG: 249894 (Sel Sweep project).Peer Reviewe