123 research outputs found
Exploratory investigation of the use of area suction to eliminate air-flow separation in diffusers having large expansion angles
Tests were made at a mean inlet Mach number of 0.2 with area suction applied to conical diffusers with expansion angles of 30 degrees and 50 degrees and exit to inlet area ratios of 2. Air-flow separation was eliminated with suction mass flows of 3 and 4 percent of the inlet mass flows for the 30 degrees and 50 degrees diffusers, respectively
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WONDY: a computer program for calculating problems of motion in one dimension
Peptidomics characterization of allergenic and non-allergenic tropomyosin orthologs
Background
The cleavage and the digestion patterns of allergenic proteins play a key role in allergenicity. The transformation of food proteins starts with their denaturation by the acidic environment of the stomach. However, protein denaturation is not enough to completely remove allergenic properties of a protein, but it is necessary a complete enzymatic digestion. Whether the enzymatic digestion is not efficient, the persistence of bigger peptides can occur and put the basis for the development of the sensitization process.The hypothesis of the current work takes into consideration the probability that shrimp tropomyosin (TM) is not fully digested or presents a digestion pattern that generates some peptides that can be immunogenic.Therefore, the work plan designed, aims to study the cleavage pattern of: purified chicken TM, recombinant chicken TM, purified TM of Penaeus monodon (Pen m 1), recombinant TM of Penaeus monodon (rPen m 1) and recombinant TM of Crangon crangon (rCrac c 1).
Methods
One mg of each ortholog has been processed through simulated mouth, gastric and intestinal digestion. The sample was frozen to block the digestion and, after this step, concentrated and cleaned through protein precipitation. The protein pellet was processed for peptidomic analysis through 1D Tricine gel electrophoresis, 2D Tricine gel electrophoresis and mass spectrometry.
Results
Simulated gastric digestion pattern of shrimp TM highlighted the presence of a resistant band at an average MW of 25 kDa that could be involved in the immunogenic process.
Conclusions
This innovative approach (peptidomics study through 1D-2D Tricine/MS) could represent a milestone for the study of digestion patterns of allergenic proteins or for the study of allergenic potential of novel foods
South Ridgeline habitat study : Final report
177 pp. Bookmarks supplied by UO. Includes maps and figures. Published August, 2007. Captured January 23, 2008.This report contains documentation of a five-month inventory and assessment of over 2600 acres of
upland habitats on public and private land in the South Ridgeline area of Eugene, Oregon. The
South Ridgeline Habitat Study (SRHS) was undertaken to document the location, quantity, and
quality of upland habitat, and suitability of habitat for uncommon and rare species in the area, and to
do so in a way that meets the inventory standards contained in the administrative rules for Statewide
Planning Goal 5.... This report contains: 1) background information about the study area, including ecological history;
2) methodology for inventory and assessment ratings; and 3) a summary of results. [From the Document
Clinical relevance of IgE to recombinant Gly m 4 in the diagnosis of adult soybean allergy
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NACA Technical Notes
Tests were made at a mean inlet Mach number of 0.2 with area suction applied to conical diffusers with expansion angles of 30 degrees and 50 degrees and exit to inlet area ratios of 2. Air-flow separation was eliminated with suction mass flows of 3 and 4 percent of the inlet mass flows for the 30 degrees and 50 degrees diffusers, respectively
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