6,116 research outputs found
The structure of an LIM-only protein 4 (LMO4) and deformed epidermal autoregulatory factor-1 (DEAF1) complex reveals a common mode of binding to LMO4
LIM-domain only protein 4 (LMO4) is a widely expressed protein with important roles in embryonic development and breast cancer. It has been reported to bind many partners, including the transcription factor Deformed epidermal autoregulatory factor-1 (DEAF1), with which LMO4 shares many biological parallels. We used yeast two-hybrid assays to show that DEAF1 binds both LIM domains of LMO4 and that DEAF1 binds the same face on LMO4 as two other LMO4-binding partners, namely LIM domain binding protein 1 (LDB1) and C-terminal binding protein interacting protein (CtIP/RBBP8). Mutagenic screening analysed by the same method, indicates that the key residues in the interaction lie in LMO4LIM2 and the N-terminal half of the LMO4-binding domain in DEAF1. We generated a stable LMO4LIM2-DEAF1 complex and determined the solution structure of that complex. Although the LMO4-binding domain from DEAF1 is intrinsically disordered, it becomes structured on binding. The structure confirms that LDB1, CtIP and DEAF1 all bind to the same face on LMO4. LMO4 appears to form a hub in protein-protein interaction networks, linking numerous pathways within cells. Competitive binding for LMO4 therefore most likely provides a level of regulation between those different pathways.SJ was funded by an Australian Postgraduate Award (education.gov.au/australian-postgraduate-awards). JPM and JMM were awarded Senior Research
Fellowships from the Australian National and Medical Research Council (www.nhmrc.gov.au). This project was funded by an Australian Research Council (www.
arc.gov.au) Discovery Project Grant (DP110104332) to JMM and LC
Order parameters in the Verwey phase transition
The Verwey phase transition in magnetite is analyzed on the basis of the
Landau theory. The free energy functional is expanded in a series of components
belonging to the primary and secondary order parameters. A low-temperature
phase with the monoclinic P2/c symmetry is a result of condensation of two
order parameters X_3 and \Delta_5 . The temperature dependence of the shear
elastic constant C_44 is derived and the mechanism of its softening is
discussed.Comment: 4 pages, 1 figur
Consequences of asteroid fragmentation during impact hazard mitigation
The consequences of the fragmentation of an Earth-threatening asteroid due to an attempted deflection are examined in this paper. The minimum required energy for a successful impulsive deflection of a threatening object is computed and compared to the energy required to break up a small size asteroid. The results show that the fragmentation of an asteroid that underwent an impulsive deflection, such as a kinetic impact or a nuclear explosion, is a very plausible event.Astatistical model is used to approximate the number and size of the fragments as well as the distribution of velocities at the instant after the deflection attempt takes place. This distribution of velocities is a function of the energy provided by the deflection attempt, whereas the number and size of the asteroidal fragments is a function of the size of the largest fragment. The model also takes into account the gravity forces that could lead to a reaggregation of the asteroid after fragmentation. The probability distribution of the pieces after the deflection is then propagated forward in time until the encounter with Earth. A probability damage factor (i.e., expected damage caused by a given size fragment multiplied by its impact probability) is then computed and analyzed for different plausible scenarios, characterized by different levels of deflection energies and lead times
Destination Management through Organizational Ambidexterity
Tourism can help regenerate post-colonial, post-conflict and post-disaster destinations (PCCDs), and national governments and destination marketing organisations (DMOs) play a central role in this. They face the dilemma of either consolidating tourism situations with seemingly safe, known, predictable steps, or taking more ambitious risk-prone, less tried-and-tested and more uncertain approaches. This choice can be portrayed as the respective exploitative and explorative dimensions of strategic conceptual framework of organisational ambidexterity (OA). This regional spotlight provides a conceptual analysis using the lens of OA to examine these dynamics. It focuses on the specific case of Haiti, set within the context of the Caribbean region. A range of OA effects in relation to tourist enclaves is identified. In particular, the spotlight argues for less segregation and separation between tourist and local populations, along with a need for DMOs to espouse more exploitative-explorative postures. In terms of wider implications, it can be argued that other Caribbean economies might learn lessons from the discussion of the Haitian case
New Approach to Nonlinear Dynamics of Fullerenes and Fullerites
New type of nonlinear (anharmonic) excitations -- bushes of vibrational modes
-- in physical systems with point or space symmetry are discussed. All infrared
active and Raman active bushes for C60 fulerene are found by means of special
group-theoretical methods.Comment: LaTeX, 8 pages, to be published in Fizika Tverdogo Tela, 200
Isospin splittings of meson and baryon masses from three-flavor lattice QCD + QED
Lattice QCD simulations are now reaching a precision where isospin breaking
effects become important. Previously, we have developed a program to
systematically investigate the pattern of flavor symmetry beaking within QCD
and successfully applied it to meson and baryon masses involving up, down and
strange quarks. In this Letter we extend the calculations to QCD + QED and
present our first results on isospin splittings in the pseudoscalar meson and
baryon octets. In particular, we obtain the nucleon mass difference of
M_n-M_p=1.35(18)(8)\,\mbox{MeV} and the electromagnetic contribution to the
pion splitting M_{\pi^+}-M_{\pi^0}=4.60(20)\,\mbox{MeV}. Further we report
first determination of the separation between strong and electromagnetic
contributions in the scheme.Comment: 14 pages, 10 figures, text and figures added, Journal versio
Hydrodynamics of confined colloidal fluids in two dimensions
We apply a hybrid Molecular Dynamics and mesoscopic simulation technique to
study the dynamics of two dimensional colloidal discs in confined geometries.
We calculate the velocity autocorrelation functions, and observe the predicted
long time hydrodynamic tail that characterizes unconfined fluids, as
well as more complex oscillating behavior and negative tails for strongly
confined geometries. Because the tail of the velocity autocorrelation
function is cut off for longer times in finite systems, the related diffusion
coefficient does not diverge, but instead depends logarithmically on the
overall size of the system.Comment: RevTex 13 pages, 9 figure
High-pressure phase and transition phenomena in ammonia borane NH3BH3 from X-ray diffraction, Landau theory, and ab initio calculations
Structural evolution of a prospective hydrogen storage material, ammonia
borane NH3BH3, has been studied at high pressures up to 12 GPa and at low
temperatures by synchrotron powder diffraction. At 293 K and above 1.1 GPa a
disordered I4mm structure reversibly transforms into a new ordered phase. Its
Cmc21 structure was solved from the diffraction data, the positions of N and B
atoms and the orientation of NH3 and BH3 groups were finally assigned with the
help of density functional theory calculations. Group-theoretical analysis
identifies a single two-component order parameter, combining ordering and
atomic displacement mechanisms, which link an orientationally disordered parent
phase I4mm with ordered distorted Cmc21, Pmn21 and P21 structures. We propose a
generic phase diagram for NH3BH3, mapping three experimentally found and one
predicted (P21) phases as a function of temperature and pressure, along with
the evolution of the corresponding structural distortions. Ammonia borane
belongs to the class of improper ferroelastics and we show that both
temperature- and pressure-induced phase transitions can be driven to be of the
second order. The role of N-H...H-B dihydrogen bonds and other intermolecular
interactions in the stability of NH3BH3 polymorphs is examined.Comment: 23 pages, 7 figure
R-modes in Neutron Stars with Crusts: Turbulent Saturation, Spin-down, and Crust Melting
Rossby waves (r-modes) have been suggested as a means to regulate the spin
periods of young or accreting neutron stars, and also to produce observable
gravitational wave radiation. R-modes involve primarily transverse,
incompressive motions of the star's fluid core. However, neutron stars gain
crusts early in their lives: therefore, r-modes also imply shear in the fluid
beneath the crust. We examine the criterion for this shear layer to become
turbulent, and derive the rate of dissipation in the turbulent regime. Unlike
dissipation from a viscous boundary layer, turbulent energy loss is nonlinear
in mode energy and can therefore cause the mode to saturate at amplitudes
typically much less than unity. This energy loss also reappears as heat below
the crust. We study the possibility of crust melting as well as its
implications for the spin evolution of low-mass X-ray binaries. Lastly, we
identify some universal features of the spin evolution that may have
observational consequences.Comment: 12 pages, 4 figures, submitted to Ap
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