351 research outputs found

    A rocket-borne Langmuir probe response to continuous and pulsed sweep modes

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    Nightime ionospheric electron density and temperature are measured using a rocket-borne Langmuir probe (LP) launched on board a SONDA III rocket from the Brazilian equatorial rocket launching station in Alcantara-MA, at 23:51 hrs (LT) on May 31, 1992. A sweep voltage varying between -IV and +2.5V is applied to the spherical LP sensor alternately in continuous and pulsed modes. In the continuous mode the effect of contamination of the sensor surface on the current collected by the sensor is clearly seen in the current-voltage characteristics and thereby on the electron temperature estimated, while this effect is practically absent in the pulsed mode operation. An electron temperature profile estimated from the LP data is compared with the IRI90 model profile.Material digitalizado en SEDICI gracias a la colaboración de la Facultad de Ciencias Astronómicas y Geofísicas (UNLP).Asociación Argentina de Geofísicos y Geodesta

    A rocket-borne Langmuir probe response to continuous and pulsed sweep modes

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    Nightime ionospheric electron density and temperature are measured using a rocket-borne Langmuir probe (LP) launched on board a SONDA III rocket from the Brazilian equatorial rocket launching station in Alcantara-MA, at 23:51 hrs (LT) on May 31, 1992. A sweep voltage varying between -IV and +2.5V is applied to the spherical LP sensor alternately in continuous and pulsed modes. In the continuous mode the effect of contamination of the sensor surface on the current collected by the sensor is clearly seen in the current-voltage characteristics and thereby on the electron temperature estimated, while this effect is practically absent in the pulsed mode operation. An electron temperature profile estimated from the LP data is compared with the IRI90 model profile.Material digitalizado en SEDICI gracias a la colaboración de la Facultad de Ciencias Astronómicas y Geofísicas (UNLP).Asociación Argentina de Geofísicos y Geodesta

    Signatures of 3?6 day planetary waves in the equatorial mesosphere and ionosphere

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    International audienceCommon periodic oscillations have been observed in meteor radar measurements of the MLT winds at Cariri (7.4° S, 36.5° W) and Ascension Island (7.9° S, 14.4° W) and in the minimum ionospheric virtual height, h'F, measured at Fortaleza (3.9° S, 38.4° W) in 2004, all located in the near equatorial region. Wavelet analysis of these time series reveals that there are 3?4-day, 6?8-day and 12?16-day oscillations in the zonal winds and h'F. The 3?4 day oscillation appeared as a form of a wave packet from 7?17 August 2004. From the wave characteristics analyzed this might be a 3.5-day Ultra Fast Kelvin wave. The 6-day oscillation in the mesosphere was prominent during the period of August to November. In the ionosphere, however, it was apparent only in November. Spectral analysis suggests that this might be a 6.5-day wave previously identified. The 3.5-day and 6.5-day waves in the ionosphere could have important roles in the initiation of equatorial spread F (plasma bubble). These waves might modulate the post-sunset E×B uplifting of the base of the F-layer via the induced lower thermosphere zonal wind and/or the E-region conductivity

    Study on the Discrimination of Possible Error Sources That Might Affect the Quality of Volatile Organic Compounds Signature in Dairy Cattle Using an Electronic Nose

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    Electronic nose devices (EN) have been developed for detecting volatile organic compounds (VOCs). This study aimed to assess the ability of the MENT-EGAS prototype-based EN to respond to direct sampling and to evaluate the influence of possible error sources that might affect the quality of VOC signatures. This study was performed on a dairy farm using 11 (n = 11) multiparous Holstein-Friesian cows. The cows were divided into two groups housed in two different barns: group I included six lactating cows fed with a lactating diet (LD), and group II included 5 non-lactating late pregnant cows fed with a far-off diet (FD). Each group was offered 250 g of their respective diet; 10 min later, exhalated breath was collected for VOC determination. After this sampling, 4 cows from each group were offered 250 g of pellet concentrates. Ten minutes later, the exhalated breath was collected once more. VOCs were also measured directly from the feed's headspace, as well as from the environmental backgrounds of each. Principal component analyses (PCA) were performed and revealed clear discrimination between the two different environmental backgrounds, the two different feed headspaces, the exhalated breath of groups I and II cows, and the exhalated breath within the same group of cows before and after the feed intake. Based on these findings, we concluded that the MENT-EGAS prototype can recognize several error sources with accuracy, providing a novel EN technology that could be used in the future in precision livestock farming

    Estrus Detection in a Dairy Herd Using an Electronic Nose by Direct Sampling on the Perineal Region

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    Estrus detection is very important for the profitability of dairy herds. Different automatic systems for estrus detection have been developed over the last decades. Our study aimed to assess the ability of the electronic nose (EN) MENT-EGAS prototype to detect estrus, based on odor release from the perineal headspace in dairy cattle by direct sampling. The study was performed in an Italian dairy farm using 35 multiparous Holstein–Friesian cows. The cows were divided into three groups: group I included 10 lactating 5-month pregnant cows, group II included 19 lactating cycling cows, and group III included 6 cows that were artificially inseminated 18 days before the trial. Odors from the perineal headspace were collected using the MENT-EGAS prototype. In group I, odors were collected once a day for 5 consecutive days. In group II, odors were collected twice daily from day 18 until day 1 of the reproductive cycle. In group III, odors were also collected twice daily from the presumable day 18 of gestation until day 22. Principal component analyses (PCA) of the perineal headspace samples were performed. PCA in group I revealed no significant discrimination. PCA in group II revealed clear discrimination between proestrus and estrus, and between estrus and metestrus but no significant discrimination was obtained between proestrus and metestrus. PCA in group III revealed that in four cows the results were similar to group I and in two cows the results were similar to group II. On day 40 of the presumable pregnancy, the ultrasound examination revealed that only the four cows were pregnant and the other two cows were regularly cycling. On the basis of our findings, we conclude that it is possible to accurately detect estrus in dairy cattle from directly collected odor samples using the MENT-EGAS prototype. This represents the first study of estrus detection using an EN detection by direct sampling. EN technologies, such as MENT-EGAS, could be applied in the future in dairy cattle farms as a precise, non-invasive method for estrus detection

    Wholly Rickettsia! Reconstructed Metabolic Profile of the Quintessential Bacterial Parasite of Eukaryotic Cells

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    Reductive genome evolution has purged many metabolic pathways from obligate intracellular Rickettsia (Alphaproteobacteria; Rickettsiaceae). While some aspects of host-dependent rickettsial metabolism have been characterized, the array of host-acquired metabolites and their cognate transporters remains unknown. This dearth of information has thwarted efforts to obtain an axenic Rickettsia culture, a major impediment to conventional genetic approaches. Using phylogenomics and computational pathway analysis, we reconstructed the Rickettsia metabolic and transport network, identifying 51 host-acquired metabolites (only 21 previously characterized) needed to compensate for degraded biosynthesis pathways. In the absence of glycolysis and the pentose phosphate pathway, cell envelope glycocon- jugates are synthesized from three imported host sugars, with a range of additional host-acquired metabolites fueling the tricarboxylic acid cycle. Fatty acid and glycero- phospholipid pathways also initiate from host precursors, and import of both iso- prenes and terpenoids is required for the synthesis of ubiquinone and the lipid car- rier of lipid I and O-antigen. Unlike metabolite-provisioning bacterial symbionts of arthropods, rickettsiae cannot synthesize B vitamins or most other cofactors, accen- tuating their parasitic nature. Six biosynthesis pathways contain holes (missing en- zymes); similar patterns in taxonomically diverse bacteria suggest alternative en- zymes that await discovery. A paucity of characterized and predicted transporters emphasizes the knowledge gap concerning how rickettsiae import host metabolites, some of which are large and not known to be transported by bacteria. Collectively, our reconstructed metabolic network offers clues to how rickettsiae hijack host met- abolic pathways. This blueprint for growth determinants is an important step toward the design of axenic media to rescue rickettsiae from the eukaryotic cell
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