The morbidity of urethral stricture disease among male Medicare beneficiaries

<p>Abstract</p> <p>Background</p> <p>To date, the morbidity of urethral stricture disease among American men has not been analyzed using national datasets. We sought to analyze the morbidity of urethral stricture disease by measuring the rates of urinary tract infections and urinary incontinence among men with a diagnosis of urethral stricture.</p> <p>Methods</p> <p>We analyzed Medicare claims data for 1992, 1995, 1998, and 2001 to estimate the rate of dual diagnoses of urethral stricture with urinary tract infection and with urinary incontinence occurring in the same year among a 5% sample of beneficiaries. Male Medicare beneficiaries receiving co-incident ICD-9 codes indicating diagnoses of urethral stricture and either urinary tract infection or urinary incontinence within the same year were counted.</p> <p>Results</p> <p>The percentage of male patients with a diagnosis of urethral stricture who also were diagnosed with a urinary tract infection was 42% in 2001, an increase from 35% in 1992. Eleven percent of male Medicare beneficiaries with urethral stricture disease in 2001 were diagnosed with urinary incontinence in the same year. This represents an increase from 8% in 1992.</p> <p>Conclusions</p> <p>Among male Medicare beneficiaries diagnosed with urethral stricture disease in 2001, 42% were also diagnosed with a urinary tract infection, and 11% with incontinence. Although the overall incidence of stricture disease decreased over this time period, these rates of dual diagnoses increased from 1992 to 2001. Our findings shed light into the health burden of stricture disease on American men. In order to decrease the morbidity of stricture disease, early definitive management of strictures is warranted.</p

Search and characterization of viral antigens able to stimulate a protective immune response against CMV infection for vaccine design

Motivation: Despite improvements in detection and treatment, human cytomegalovirus (CMV) infection is the main cause of morbi-mortality in solid organ transplant (SOT) recipients. Several efforts have been made to develop a vaccine to prevent CMV infection. However, no vaccine had been licenced to date. Efforts are necessary to identify antigens able to stimulate an immune response to protect against CMV infection.Methods: We used serum (from SOT recipients with previous CMV infection and neutralizing antibody titers &gt;480) as primary antibody to identify proteins from CMV-infected cell lysates, not recognized by the serum from the same patient with no neutralizing antibodies or in the mock-infected lysate. The identified bands were sequenced by mass spectrometry. The gene of the identified candidates was amplified by PCR using specific primers and cloned into the expression plasmids PTYB21 (fused to intein tail to purify the recombinant protein) and into pCDNA plasmid (for DNA vaccine). The PTYB21 construct was transformed into the E. coli BL21 Rosetta strain for protein expression. Protein purification was performed using the IMPACT kit (BioLabs) using a chitin affinity column. The pcDNA construct was transformed into the E. coli TOP-10 strain. All constructs were sequenced. Protein antigenicity was analyzed using VAxiJen informatic tool (threshold 0.4).Results: We identified three proteins with 135, 70 and 50 kDa approximately. The 48 kDa candidate predicted to be from the tegument and with an antigenicity score of 0.520 was selected. A 1200pb band corresponding to the predicted size of the gene was amplified. Due to difficulties of blunt ligations we introduced a dephosphorylation step of the insert before ligation. We obtained 3 positive clones with the following pattern: a band of 1280 bp corresponding to the insert and a 7514 bp for the PTYB21 plasmid and 5400 bp for the pCDNA plasmid. Sequencing of positive clones showed a 100% homology. The 48 kDa protein had the highest expression using 0.5 mM IPTG for 6 hours, and was isolated in the insoluble fraction. We are in the process of optimizing the conditions for protein purification.Conclusions:The use of patient sera is an optimal approach to identify proteins that induce an specific immune response against CMV that may be candidates for vaccine design. The process for protein purification must be optimized for each candidate since depends on multiple factors such as size and solubility

High energy protons from PKS 1333-33

In this letter we give an account of the possible acceleration of protons in the outer radio lobes of the active galaxy PKS 1333-33. We also make estimates of the arrival energy spectrum.Comment: 3 pages revtex, two figures, to appear in Mod. Phys. Lett.

An X-ray study of the SNR G344.7-0.1 and the central object CXOU J170357.8-414302

Aims. We report results of an X-ray study of the supernova remnant (SNR) G344.7-0.1 and the point-like X-ray source located at the geometrical center of the SNR radio structure. Methods. The morphology and spectral properties of the remnant and the central X-ray point-like source were studied using data from the XMM-Newton and Chandra satellites. Archival radio data and infrared Spitzer observations at 8 and 24 $\mu$m were used to compare and study its multi-band properties at different wavelengths. Results. The XMM-Newton and Chandra observations reveal that the overall X-ray emission of G344.7-0.1 is extended and correlates very well with regions of bright radio and infrared emission. The X-ray spectrum is dominated by prominent atomic emission lines. These characteristics suggest that the X-ray emission originated in a thin thermal plasma, whose radiation is represented well by a plane-parallel shock plasma model (PSHOCK). Our study favors the scenario in which G344.7-0.1 is a 6 x 10^3 year old SNR expanding in a medium with a high density gradient and is most likely encountering a molecular cloud on the western side. In addition, we report the discovery of a soft point-like X-ray source located at the geometrical center of the radio SNR structure. The object presents some characteristics of the so-called compact central objects (CCO). However, its neutral hydrogen absorption column (N_{H}) is inconsistent with that of the SNR. Coincident with the position of the source, we found infrared and optical objects with typical early-K star characteristics. The X-ray source may be a foreground star or the CCO associated with the SNR. If this latter possibility were confirmed, the point-like source would be the farthest CCO detected so far and the eighth member of the new population of isolated and weakly magnetized neutron stars.Comment: 9 pages, 8 figures, accepted for publication in Astronomy and Astrophysics. Higher resolution figures can be seen on A&

USO Y DEPENDENCIA DE LAS REMESAS EXTERIOR EN LA COMUNIDAD UNISUCREÑA: USE AND DEPENDENCE OF FOREIGN REMITTANCES IN THE UNISUCREAN COMMUNITY

Las Remesas además de haberse convertido en un rubro importante dentro de la economía de los países ya que contribuyen a reducir los niveles de pobreza siempre y cuando su uso se canalice a una forma productiva. Este trabajo busca analizar el nivel de dependencia económica de los miembros de la comunidad Unisucreña desde las remesas, a través de la caracterización de éstas como herramienta de inclusión financiera, para lo cual se tomaron datos recolectados mediante encuestas aplicadas a la comunidad Unisucreña, complementado con los diferentes informes presentados por las entidades financieras y los aportes realizados por referentes teóricos acerca de este tema. Como resultado que es a pesar de ser baja la población de la comunidad que recibe remesas, se encuentra que de éste, el 57% las utiliza para suplir las necesidades primarias del hogar. Se debe propender, a que el uso de las remesas sea más productivos de tal forma que permita generar más renta en los hogares beneficiarios

Visible and near-infrared observations of asteroid 2012 DA14 during its closest approach of February 15, 2013

Near-Earth asteroid 2012 DA14 made its closest approach on February 15, 2013, when it passed at a distance of 27,700 km from the Earth's surface. It was the first time an asteroid of moderate size was predicted to approach that close to the Earth, becoming bright enough to permit a detailed study from ground-based telescopes. Asteroid 2012 DA14 was poorly characterized before its closest approach. We acquired data using several telescopes on four Spanish observatories: the 10.4m Gran Telescopio Canarias (GTC) and the 3.6m Telescopio Nazionale Galileo (TNG), both in the El Roque de los Muchachos Observatory (ORM, La Palma); the 2.2m CAHA telescope, in the Calar Alto Observatory (Almeria); the f/3 0.77m telescope in the La Hita Observatory (Toledo); and the f/8 1.5m telescope in the Sierra Nevada Observatory (OSN, Granada). We obtained visible and near-infrared color photometry, visible spectra and time-series photometry. Visible spectra together with color photometry of 2012 DA14 show that it can be classified as an L-type asteroid, a rare spectral type with a composition similar to that of carbonaceous chondrites. The time-series photometry provides a rotational period of 8.95 +- 0.08 hours after the closest approach, and there are indications that the object suffered a spin-up during this event. The large amplitude of the light curve suggests that the object is very elongated and irregular, with an equivalent diameter of around 18m. We obtain an absolute magnitude of H_R = 24.5 +- 0.2, corresponding to H_V = 25.0 +- 0.2. The GTC photometry also gives H_V = 25.29 +- 0.14. Both values agree with the value listed at the Minor Planet Center shortly after discovery. From the absolute photometry, together with some constraints on size and shape, we compute a geometric albedo of p_V = 0.44 +- 0.20, which is slightly above the range of albedos known for L-type asteroids (0.082 - 0.405).Comment: 7 pages, 4 figures, 1 table. Accepted in A&A (June 17 2013

EEMD-MUSIC-Based Analysis for Natural Frequencies Identification of Structures Using Artificial and Natural Excitations

This paper presents a new EEMD-MUSIC- (ensemble empirical mode decomposition-multiple signal classification-) based methodology to identify modal frequencies in structures ranging from free and ambient vibration signals produced by artificial and natural excitations and also considering several factors as nonstationary effects, close modal frequencies, and noisy environments, which are common situations where several techniques reported in literature fail. The EEMD and MUSIC methods are used to decompose the vibration signal into a set of IMFs (intrinsic mode functions) and to identify the natural frequencies of a structure, respectively. The effectiveness of the proposed methodology has been validated and tested with synthetic signals and under real operating conditions. The experiments are focused on extracting the natural frequencies of a truss-type scaled structure and of a bridge used for both highway traffic and pedestrians. Results show the proposed methodology as a suitable solution for natural frequencies identification of structures from free and ambient vibration signals

Inter-Individual variability in insulin response after grape pomace supplementation in subjects at high cardiometabolic risk: role of microbiota and miRNA

Scope Dietary polyphenols have shown promising effects in mechanistic and preclinical studies on the regulation of cardiometabolic alterations. Nevertheless, clinical trials have provided contradictory results, with high inter‐individual variability. This study explores the role of gut microbiota and microRNAs (miRNAs) as factors contributing to the inter‐individual variability in polyphenol response. Methods and Results 49 subjects with at least two factors of metabolic syndrome are divided between responders (n = 23) or non‐responders (n = 26), depending on the variation rate in fasting insulin after grape pomace supplementation (6 weeks). The populations of selected fecal bacteria are estimated from fecal deoxyribonucleic acid (DNA) by quantitative real‐time polymerase chain reaction (qPCR), while the microbial‐derived short‐chain fatty acids (SCFAs) are measured in fecal samples by gas chromatography. MicroRNAs are analyzed on a representative sample, followed by targeted miRNA analysis. Responder subjects show significantly lower (p < 0.05) Prevotella and Firmicutes levels, and increased (p < 0.05) miR‐222 levels. Conclusion After evaluating the selected substrates for Prevotella and target genes of miR‐222, these variations suggest that responders are those subjects exhibiting impaired glycaemic control. This study shows that fecal microbiota and miRNA expression may be related to inter‐individual variability in clinical trials with polyphenols