The Bering Sea ice cover during March 1979: Comparison of surface and satellite data with the Nimbus-7 SMMR

During March 1979, field operations were carried out in the Marginal Ice Zone (MIZ) of the Bering Sea. The field measurements which included oceanographic, meteorological and sea ice observations were made nearly coincident with a number of Nimbus-7 and Tiros-N satellite observations. The results of a comparison between surface and aircraft observations, and images from the Tiros-N satellite, with ice concentrations derived from the microwave radiances of the Nimbus-7 Scanning Multichannel Microwave Radiometer (SMMR) are given. Following a brief discussion of the field operations, including a summary of the meteorological conditions during the experiment, the satellite data is described with emphasis on the Nimbus-7 SMMR and the physical basis of the algorithm used to retrieve ice concentrations

Scientific basis for safely shutting in the Macondo Well after the April 20, 2010 Deepwater Horizon blowout

As part of the government response to the Deepwater Horizon blowout, a Well Integrity Team evaluated the geologic hazards of shutting in the Macondo Well at the seafloor and determined the conditions under which it could safely be undertaken. Of particular concern was the possibility that, under the anticipated high shut-in pressures, oil could leak out of the well casing below the seafloor. Such a leak could lead to new geologic pathways for hydrocarbon release to the Gulf of Mexico. Evaluating this hazard required analyses of 2D and 3D seismic surveys, seafloor bathymetry, sediment properties, geophysical well logs, and drilling data to assess the geological, hydrological, and geomechanical conditions around the Macondo Well. After the well was successfully capped and shut in on July 15, 2010, a variety of monitoring activities were used to assess subsurface well integrity. These activities included acquisition of wellhead pressure data, marine multichannel seismic pro- files, seafloor and water-column sonar surveys, and wellhead visual/acoustic monitoring. These data showed that the Macondo Well was not leaking after shut in, and therefore, it could remain safely shut until reservoir pressures were suppressed (killed) with heavy drilling mud and the well was sealed with cement

Structural Insights into Rational Design of Single-Domain Antibody-Based Antitoxins against Botulinum Neurotoxins.

Botulinum neurotoxin (BoNT) is one of the most acutely lethal toxins known to humans, and effective treatment for BoNT intoxication is urgently needed. Single-domain antibodies (VHH) have been examined as a countermeasure for BoNT because of their high stability and ease of production. Here, we investigate the structures and the neutralization mechanisms for six unique VHHs targeting BoNT/A1 or BoNT/B1. These studies reveal diverse neutralizing mechanisms by which VHHs prevent host receptor binding or block transmembrane delivery of the BoNT protease domain. Guided by this knowledge, we design heterodimeric VHHs by connecting two neutralizing VHHs via a flexible spacer so they can bind simultaneously to the toxin. These bifunctional VHHs display much greater potency in a mouse co-intoxication model than similar heterodimers unable to bind simultaneously. Taken together, our studies offer insight into antibody neutralization of BoNTs and advance our ability to design multivalent anti-pathogen VHHs with improved therapeutic properties

Suite of simple metrics reveals common movement syndromes across vertebrate taxa

ecause empirical studies of animal movement are most-often site- and species-specific, we lack understanding of the level of consistency in movement patterns across diverse taxa, as well as a framework for quantitatively classifying movement patterns. We aim to address this gap by determining the extent to which statistical signatures of animal movement patterns recur across ecological systems. We assessed a suite of movement metrics derived from GPS trajectories of thirteen marine and terrestrial vertebrate species spanning three taxonomic classes, orders of magnitude in body size, and modes of movement (swimming, flying, walking). Using these metrics, we performed a principal components analysis and cluster analysis to determine if individuals organized into statistically distinct clusters. Finally, to identify and interpret commonalities within clusters, we compared them to computer-simulated idealized movement syndromes representing suites of correlated movement traits observed across taxa (migration, nomadism, territoriality, and central place foraging)

Performance Evaluation of a 1.5-kWdc a-Si PV Array Using the PVUSA Power Rating Method at NREL's Outdoor Test Facility

As part of the work conducted in the PV Systems Reliability and Performance R&D Task, a 1.5-kWdc photovoltaic (PV) array consisting of 36 Solarex MST-43MV dual-junction a-Si modules was installed and its performance monitored for almost six years (September 1999 through May 2005) at the National Renewable Energy Laboratory (NREL) Outdoor Test Facility (OTF). This paper describes the system and its performance based on the PV for Utility-Scale Applications (PVUSA) power rating method