Drycore Appears to Result from an Interaction between Rhizoctonia solani and Wireworm ( Agriotes ssp.)—Evidence from a 3-Year Field Survey

Drycore is an important quality deficiency in Europe especially in organic potato production and after grass clover leys. The drycore symptom is attributed to Rhizoctonia solani Kühn (teleomorph: Thanatephorus cucumeris (Frank) Donk). In the framework of a 3-year survey (2001-2003), data concerning potato quality, crop rotation, management, and site parameters were analysed from 185 potato fields. The hypothesis was tested that injuries on potatoes caused by wireworms facilitate the penetration of R. solani and favour the formation of drycore. Analysis of variance showed a significant influence of wireworm damage, seed quality, and grass clover leys in the crop on the level of drycore damage. On fields which had both a low occurrence of black scurf on the seed tubers and a low occurrence of wireworm damage at harvest, significant drycore damage was never observed. The relative risk for drycore damage on tubers was significantly higher if black scurf or wireworm damage was on the same tuber. In contrast, no higher risk for drycore was observed on tubers with slug damage. Abiotic factors like farm manure application, organic matter content, texture, and pH of the soil also had no significant influence on the level of drycore. Thus, the wounding of potatoes by wireworm could be confirmed as the major variable for drycore. The mode of action has to be clarified under controlled condition

Orally active antischistosomal early leads identified from the open access malaria box.

BACKGROUND: Worldwide hundreds of millions of schistosomiasis patients rely on treatment with a single drug, praziquantel. Therapeutic limitations and the threat of praziquantel resistance underline the need to discover and develop next generation drugs. METHODOLOGY: We studied the antischistosomal properties of the Medicines for Malaria Venture (MMV) malaria box containing 200 diverse drug-like and 200 probe-like compounds with confirmed in vitro activity against Plasmodium falciparum. Compounds were tested against schistosomula and adult Schistosoma mansoni in vitro. Based on in vitro performance, available pharmacokinetic profiles and toxicity data, selected compounds were investigated in vivo. PRINCIPAL FINDINGS: Promising antischistosomal activity (IC50: 1.4-9.5 µM) was observed for 34 compounds against schistosomula. Three compounds presented IC50 values between 0.8 and 1.3 µM against adult S. mansoni. Two promising early leads were identified, namely a N,N'-diarylurea and a 2,3-dianilinoquinoxaline. Treatment of S. mansoni infected mice with a single oral 400 mg/kg dose of these drugs resulted in significant worm burden reductions of 52.5% and 40.8%, respectively. CONCLUSIONS/SIGNIFICANCE: The two candidates identified by investigating the MMV malaria box are characterized by good pharmacokinetic profiles, low cytotoxic potential and easy chemistry and therefore offer an excellent starting point for antischistosomal drug discovery and development

Supernova-Triggered Molecular Cloud Core Collapse and the Rayleigh-Taylor Fingers that Polluted the Solar Nebula

A supernova is a likely source of short-lived radioisotopes (SLRIs) that were present during the formation of the earliest solar system solids. A suitably thin and dense supernova shock wave may be capable of triggering the self-gravitational collapse of a molecular cloud core while simultaneously injecting SLRIs. Axisymmetric hydrodynamics models have shown that this injection occurs through a number of Rayleigh-Taylor (RT) rings. Here we use the FLASH adaptive mesh refinement (AMR) hydrodynamics code to calculate the first fully three dimensional (3D) models of the triggering and injection process. The axisymmetric RT rings become RT fingers in 3D. While ~ 100 RT fingers appear early in the 3D models, only a few RT fingers are likely to impact the densest portion of the collapsing cloud core. These few RT fingers must then be the source of any SLRI spatial heterogeneity in the solar nebula inferred from isotopic analyses of chondritic meteorites. The models show that SLRI injection efficiencies from a supernova several pc away fall at the lower end of the range estimated for matching SLRI abundances, perhaps putting them more into agreement with recent reassessments of the level of 60Fe present in the solar nebula.Comment: 14 pages, 4 figures, 1 table, in press, ApJ Letter

Switching to second-line antiretroviral therapy in resource-limited settings: comparison of programmes with and without viral load monitoring.

In high-income countries, viral load is routinely measured to detect failure of antiretroviral therapy (ART) and guide switching to second-line ART. Viral load monitoring is not generally available in resource-limited settings. We examined switching from nonnucleoside reverse transcriptase inhibitor (NNRTI)-based first-line regimens to protease inhibitor-based regimens in Africa, South America and Asia