EXECUTE IOT-BASED HEALTHCARE SOLUTIONS BASED ON CLOUD COMPUTING

Patient monitoring arrangements are gaining their relevance as the fast-growing international aged public increases demands for sympathetic. These structures use cellular technologies to relay vital sign for therapeutic interpretation. The aim of the proposed sniff out arranges a correct strength care to folk from the company in more commercial and pertinent affable practice. The need of home situated fitness monitoring structure is elevated nowadays for the sake of strength care cost is developing exponentially in last few decenniums. In the planned home occupying well-being monitoring structure applying cyber resourceful dial includes the aspects of return of therapeutic parameters like Body condition, Pulse rate, and ECG. Processing of a poised data practicing ARM7 (LPC2148) processor and prepared data is then spread out on doctors or relatives hominoid mobile buzz. Also, the data perhaps spread out on workstation. The technique is utilizing a competitive unit to carry data like ECG to MD for monitoring; interpretation and patients care at kind of economical, withal patient’s location

Splicing factor ESRP1 controls ER-positive breast cancer by altering metabolic pathways

The epithelial splicing regulatory proteins 1 and 2 (ESRP1 and ESRP2) control the epithelial-to-mesenchymal transition (EMT) splicing program in cancer. However, their role in breast cancer recurrence is unclear. In this study, we report that high levels of ESRP1, but not ESRP2, are associated with poor prognosis in estrogen receptor positive (ER+) breast tumors. Knockdown of ESRP1 in endocrine-resistant breast cancer models decreases growth significantly and alters the EMT splicing signature, which we confirm using TCGA SpliceSeq data of ER+ BRCA tumors. However, these changes are not accompanied by the development of a mesenchymal phenotype or a change in key EMT-transcription factors. In tamoxifen-resistant cells, knockdown of ESRP1 affects lipid metabolism and oxidoreductase processes, resulting in the decreased expression of fatty acid synthase (FASN), stearoyl-CoA desaturase 1 (SCD1), and phosphoglycerate dehydrogenase (PHGDH) at both the mRNA and protein levels. Furthermore, ESRP1 knockdown increases the basal respiration and spare respiration capacity. This study reports a novel role for ESRP1 that could form the basis for the prevention of tamoxifen resistance in ER+ breast cancer

Expression levels of SF3B3 correlate with prognosis and endocrine resistance in estrogen receptor-positive breast cancer

De novo or acquired resistance to endocrine therapy limits its utility in a significant number of estrogen receptor-positive (ER-positive) breast cancers. It is crucial to identify novel targets for therapeutic intervention and improve the success of endocrine therapies. Splicing factor 3b, subunit 1 (SF3B1) mutations are described in luminal breast cancer albeit in low frequency. In this study, we evaluated the role of SF3B1 and SF3B3, critical parts of the SF3b splicing complex, in ER-positive endocrine resistance. To ascertain the role of SF3B1/SF3B3 in endocrine resistance, their expression levels were evaluated in ER-positive/endocrine-resistant cell lines (MCF-7/LCC2 and MCF-7/LCC9) using a real-time quantitative reverse transcription PCR (qRT-PCR). To further determine their clinical relevance, expression analysis was performed in a cohort of 60 paraffin-embedded ER-positive, node-negative breast carcinomas with low, intermediate, and high Oncotype DX recurrence scores. Expression levels of SF3B1 and SF3B3 and their prognostic value were validated in large cohorts using publicly available gene expression data sets including The Cancer Genome Atlas. SF3B1 and SF3B3 levels were significantly increased in ERα-positive cells with acquired tamoxifen (MCF-7/LCC2; both P<0.0002) and fulvestrant/tamoxifen resistance (MCF-7/LCC9; P=0.008 for SF3B1 and P=0.0006 for SF3B3). Expression levels of both MCF-7/LCC2 and MCF-7/LCC9 were not affected by additional treatments with E2 and/or tamoxifen. Furthermore, qRT-PCR analysis confirmed that SF3B3 expression is significantly upregulated in Oncotype DX high-risk groups when compared with low risk (P=0.019). Similarly, in publicly available breast cancer gene expression data sets, overexpression of SF3B3, but not SF3B1, was significantly correlated with overall survival. Furthermore, the correlation was significant in ER-positive, but not in ER-negative tumors

EFFECT OF DATA QUALITY ON WATER BODY SEGMENTATION WITH DEEPLABV3+ ALGORITHM

Training Deep Learning (DL) algorithms for segmenting features require hundreds to thousands of input data and corresponding labels. Generating thousands of input images and labels requires considerable resources and time. Hence, it is common practice to use opensource imagery data and labels available online. Most of these open-source data have little or no metadata describing their quality or suitability making it problematic for training or evaluating DL models. This study evaluated the effect of data quality on training DeepLabV3+, using Sentinel 2 A/B RGB images and labels obtained from Kaggle. We generated subsets of 256&thinsp;&times;&thinsp;256 pixels, and 10% of these images (802) were set aside for testing. First, we trained and validated the DeepLabV3+ model with the remaining images. Second, we removed images with incorrect labels and trained another DeepLabV3+ network. Finally, we trained the third DeepLabV3+ network after removing images with turbid water or with floating vegetation. All three trained models were evaluated with test images and then we calculated accuracy metrics. As the quality of the input images improved, accuracy of the predicted masks generated from the first model increased from 92.8% to 94.3% in the second model. The third model’s accuracy was 96.4%, demonstrating the network’s ability to better learn and predict water bodies when the input data had fewer class variations. Based on the results we recommend assessing the quality of open-source data for incorrect labels and variations in the target class prior to training DeepLabV3+ or any other DL network

Immunohistochemical Expression of Anaplastic Lymphoma Kinase in Invasive Breast Carcinomas- A Retrospective Study

Introduction: Breast carcinoma remains one of the most common causes of mortality among female cancer patients inspite of improvements in treatment modalities. Increased survival rate can be achieved by identification of new targeted therapies. Anaplastic Lymphoma Kinase (ALK) alterations are present in many solid and haematological tumours indicating its role in pathogenesis and treatment. There are studies regarding the expression of ALK in few breast cancers but its importance was not clearly mentioned. Hence, identification of ALK overexpression in breast cancers, particularly in Triple Negative Breast Cancers (TNBC) might play a role in their chemotherapy with the help of ALK inhibitors. Aim: To study the ALK expression in different subtypes of invasive carcinomas of breast. Materials and Methods: This was a retrospective cross-sectional study conducted in the Department of Pathology at Great Eastern Medical School (GEMS) and Hospital, Srikakulam, Andhra Pradesh, India, from January 2022 to July 2022. The data of 60 patients, from January 2021 to December 2021 was retrieved using Hospital Information Management System (HIMS) and the Haematoxylin and Eosin (H&E)-stained slides and formalin fixed paraffin embedded tissue blocks of breast tumour were retrieved and reviewed. Estrogen receptor (ER), Progesterone receptor (PR) and Human Epidermal growth factor Receptor 2 (HER2) immunostains were performed and categorised based on molecular classification as Luminal, Her2 and Triple negative. ALK Staining was performed on all cases and its expression was studied. Statistical Package for Social Sciences (SPSS) software version 2.0 was used for analysis of data. Mean with standard deviation is used for quantitative variables and prevalence, ratio is used for quantitative variables. Chi-square test and Fischer exact test were used for detecting significance. The p-value <0.05 was considered as significant. Results: Out of 60 cases, majority (N=22; 36.66%) of patients were in the age group of 51-60 years. The mean tumour size was 3.2±0.5 cm. The most common histological type was invasive breast carcinoma, No Special Type (N=44; 73.34%). Majority of the tumours showed grade 1 and 2 with 24 (40%) and 25 (41.67%) cases, respectively. A total of 30 cases (50%) of tumours belonged to stage T2. Luminal molecular subtype was the most common 31 (51.67%) cases followed by TNBC, 16 (26.67%) cases and Her2neu 13 (21.67%) cases. Among all the cases, ALK overexpression was seen in 17 (28.33%) cases and among different molecular subtypes, its expression was seen in 5 (8.33%) cases of Luminal type, 3 (5.0%) cases of HER2 type and in 9 (15.0%) cases of TNBC cases. Conclusion: Immunohistochemical analysis showed ALK over expression in a substantial proportion of cases and possibly plays a significant role in aggressive behaviour of breast cancer. ALK inhibitors offer an opportunity to treat aggressive subtypes of breast cancer

Review on Various Applications of Visible Light Communication

In the current scenario, there is an increased usage of Radio Frequency (RF) for wireless communication, that there is a major problem of bandwidth allocation. In order to overcome this problem a new technology called Visible Light Communication (VLC) is introduced which uses visible light whose data rates are higher than radio waves. Visible Light requires fewer components than radio technology, hence cost effective. Data transmission requires LED light sources which consumes less power. The data transmitted is more secure and can be used in electromagnetic sensitive areas. This transmission technique can also be used in aircraft cabins, hospitals and nuclear power plants without causing electromagnetic interference

Effects of Single and Integrated Water, Sanitation, Handwashing, and Nutrition Interventions on Child Soil-Transmitted Helminth and Giardia infections: A Cluster-Randomized Controlled Trial in Rural Kenya

Helminth and protozoan infections affect more than 1 billion children globally. Improving water quality, sanitation, handwashing, and nutrition could be more sustainable control strategies for parasite infections than mass drug administration, while providing other quality of life benefits

Scoring System Approach for Assessment of Critical Illness using Mobile Phones

Abstrac

An in vitro comparative evaluation of the effect of three intracanal medicaments – Chlorhexidine gel, triple antibiotic paste, and calcium hydroxide paste on the push-out bond strength of MTA Plus, Biodentine, and calcium-enriched mixture

Aim: Caries or traumatic injuries affecting young permanent teeth during root development usually result in an open apex which is highly challenging to treat. The aim of this study was to evaluate the push-out bond strength of apical plugs of Mineral Trioxide Aggregate (MTA) Plus, Biodentine, and calcium-enriched mixture (CEM) after premedication with chlorhexidine, triple antibiotic paste (TAP), and calcium hydroxide. Methods: Fifty-four extracted intact anterior teeth were decoronated and 3 mm was sectioned from the apex. The canals were rendered parallel using #80 K-files and #3, #4 Peeso reamers to mimic an open apex situation. The samples were divided into 3 groups containing 18 samples each for the three intracanal medicaments. After premedication, the samples were stored for 2 weeks at 37°C, following which the canals were cleaned using #80 K-file along with ethylenediaminetetraacetic acid and sodium hypochlorite irrigation. The samples were further divided into three subgroups, each containing six samples for the three calcium silicate cement used in this study. They were later subjected to push-out bond strength testing. Results: Regardless of the type of intracanal medicament used, Biodentine had significantly higher bond strength than MTA Plus and CEM. The highest push-out bond strength results were obtained in samples premedicated with chlorhexidine. Compared to TAP and calcium hydroxide (Ca(OH) 2), this value was statistically significant (P < 0.001). Conclusion: Within the limitations of this study, Biodentine showed the highest push-out bond strength as compared to MTA Plus and CEM. Chlorhexidine may be superior to calcium hydroxide and TAP medicaments when used for apexification procedures with calcium silicate cement

Design, synthesis and characterization of novel paracetamol derivatives to target breast cancer

1257-1272Most breast cancers are Estrogen Receptor-positive type. In the mammary epithelial, estrogen controls many cellular activities such as proliferation, differentiation and migration. There are two genetically distinct and functional estrogen receptors (ERs), ERα and ERβ, belonging to the superfamily of nuclear receptors for steroid/thyroid harmones. Estrogenexert its functions in different tissues by binding with its receptors, including alpha and beta (ERα and ERβ). Estrogen Receptor alpha (ERα) controls breast tissue development and progression of breast cancer. Paracetamol is one of the most widely used medicines. A recent experimental study suggests that paracetamol may have several pharmacological effects other than its well known analgesic/antipyretic properties. The docking study was performed on different paracetamol derivatives using Schrodinger 2015 (maestro 10.1) on Human Estrogen Receptor Alpha Ligand-Binding Domain (1XP6) and Endothelial nitric oxide synthase (3NLE). The in silico studies indicate that N-(4-((1H-1,2,3-triazol-4-yl)methoxy)phenyl) acetamide derivatives exhibit comparable docking score and good hydrogen bond interactions at Ligand binding domain of ERα and 3NLE. Based on the docking studies, a new series of N-(4-((1H-1,2,3-triazol-4-yl)methoxy)phenyl) acetamide derivatives have been synthesized by employing click chemistry approach. Nine compounds have been evaluated for their cytotoxicity in MCF-7 cell line and anti oxidant activity. Many of the synthesized compounds exhibit potent cytotoxic and anti oxidant activity. In particular 5c, 5g, and 5b compounds show most potent cytotoxicity with IC50 value of 19.83, 20.57, 20.83 µg/mL respectively and 5e and 5f show most potent anti oxidant activity with IC50 value of 0.4, 0.5 µg/mL respectively