Human tRNAGlu genes: their copy number and organisation

AbstractThe tRNAGlu gene copy number, determined by genomic blot analysis of human plaeental DNA, is approximately thirteen. These studies, using several probes and DNA digested with several restriction enzymes singly or in combination, show that most of these tRNAGlu genes are flanked by DNA of very similar sequence for at least 5 kb. This conclusion is supported by the close similarity of the restriction maps of two λ Charon-4A recombinants of human genomic DNA containing two different tRNAglu genes

Biological Monitoring of Hexavalent Chromium and Serum Levels of the Senescence Biomarker Apolipoprotein J/Clusterin in Welders

Welding fumes contain metals and other toxic substances known or strongly suspected to be related with oxidative stress and premature cellular senescence. Apolipoprotein J/Clusterin (ApoJ/CLU) is a glycoprotein that is differentially regulated in various physiological and disease states including ageing and age-related diseases. In vitro data showed that exposure of human diploid fibroblasts to hexavalent chromium (Cr(VI)) resulted in premature senescence and significant upregulation of the ApoJ/CLU protein. In this study we analyzed blood and urine samples from shipyard industry welders being exposed to different levels of Cr(VI) over a period of five months in order to assay in vivo the relation of ApoJ/CLU serum levels with Cr(VI). Our findings confirmed the previously reported in vitro data since reduction of Cr levels, after a worksite intervention, associated with lower levels of ApoJ/CLU serum levels. We concluded that the human ApoJ/CLU gene is responsive to the acute in vivo oxidative stress induced by heavy metals such as hexavalent chromium

Persistence in systems with conserved order parameter

We consider the low-temperature coarsening dynamics of a one-dimensional Ising ferromagnet with conserved Kawasaki-like dynamics in the domain representation. Domains diffuse with size-dependent diffusion constant, $D(l) \propto l^\gamma$ with $\gamma = -1$. We generalize this model to arbitrary $\gamma$, and derive an expression for the domain density, $N(t) \sim t^{-\phi}$ with $\phi=1/(2-\gamma)$, using a scaling argument. We also investigate numerically the persistence exponent $\theta$ characterizing the power-law decay of the number, $N_p(t)$, of persistent (unflipped) spins at time $t$, and find $N_{p}(t)\sim t^{-\theta}$ where $\theta$ depends on $\gamma$. We show how the results for $\phi$ and $\theta$ are related to similar calculations in diffusion-limited cluster-cluster aggregation (DLCA) where clusters with size-dependent diffusion constant diffuse through an immobile `empty' phase and aggregate irreversibly on impact. Simulations show that, while $\phi$ is the same in both models, $\theta$ is different except for $\gamma=0$. We also investigate models that interpolate between symmetric domain diffusion and DLCA.Comment: 9 pages, minor revision

Survival of a Diffusing Particle in a Transverse Shear Flow: A First-Passage Problem with Continuously Varying Persistence Exponent

We consider a particle diffusing in the y-direction, dy/dt=\eta(t), subject to a transverse shear flow in the x-direction, dx/dt=f(y), where x \ge 0 and x=0 is an absorbing boundary. We treat the class of models defined by f(y) = \pm v_{\pm}(\pm y)^\alpha where the upper (lower) sign refers to y>0 (y<0). We show that the particle survives with probability Q(t) \sim t^{-\theta} with \theta = 1/4, independent of \alpha, if v_{+}=v_{-}. If v_{+} \ne v_{-}, however, we show that \theta depends on both \alpha and the ratio v_{+}/v_{-}, and we determine this dependence.Comment: 4 page

Analysis of the machinery and intermediates of the 5hmC-mediated DNA demethylation pathway in aging on samples from the MARKAGE Study

Gradual changes in the DNA methylation landscape occur throughout aging virtually in all human tissues. A widespread reduction of 5-methylcytosine (5mC), associated with highly reproducible site-specific hypermethylation, characterizes the genome in aging. Therefore, an equilibrium seems to exist between general and directional deregulating events concerning DNA methylation controllers, which may underpin the age-related epigenetic changes. In this context, 5mC-hydroxylases (TET enzymes) are new potential players. In fact, TETs catalyze the stepwise oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), driving the DNA demethylation process based on thymine DNA glycosylase (TDG)-mediated DNA repair pathway. The present paper reports the expression of DNA hydroxymethylation components, the levels of 5hmC and of its derivatives in peripheral blood mononuclear cells of age-stratified donors recruited in several European countries in the context of the EU Project ‘MARK-AGE’. The results provide evidence for an age-related decline of TET1, TET3 and TDG gene expression along with a decrease of 5hmC and an accumulation of 5caC. These associations were independent of confounding variables, including recruitment center, gender and leukocyte composition. The observed impairment of 5hmC-mediated DNA demethylation pathway in blood cells may lead to aberrant transcriptional programs in the elderly

Age-dependent expression of DNMT1 and DNMT3B in PBMCs from a large European population enrolled in the MARK-AGE study

Aging is associated with alterations in the content and patterns of DNA methylation virtually throughout the entire human lifespan. Reasons for these variations are not well understood. However, several lines of evidence suggest that the epigenetic instability in aging may be traced back to the alteration of the expression of DNA methyltransferases. Here, the association of the expression of DNA methyltransferases DNMT1 and DNMT3B with age has been analysed in the context of the MARK-AGE study, a large-scale cross-sectional study of the European general population. Using peripheral blood mononuclear cells, we assessed the variation of DNMT1 and DNMT3B gene expression in more than two thousand age-stratified women and men (35-75 years) recruited across eight European countries. Significant age-related changes were detected for both transcripts. The level of DNMT1 gradually dropped with aging but this was only observed up to the age of 64 years. By contrast, the expression of DNMT3B decreased linearly with increasing age and this association was particularly evident in females. We next attempted to trace the age-related changes of both transcripts to the influence of different variables that have an impact on changes of their expression in the population, including demographics, dietary and health habits, and clinical parameters. Our results indicate that age affects the expression of DNMT1 and DNMT3B as an almost independent variable in respect of all other variables evaluated

The writing on the wall: the concealed communities of the East Yorkshire horselads

This paper examines the graffiti found within late nineteenth and early-twentieth century farm buildings in the Wolds of East Yorkshire. It suggests that the graffiti were created by a group of young men at the bottom of the social hierarchy - the horselads – and was one of the ways in which they constructed a distinctive sense of communal identity, at a particular stage in their lives. Whilst it tells us much about changing agricultural regimes and social structures, it also informs us about experiences and attitudes often hidden from official histories and biographies. In this way, the graffiti are argued to inform our understanding, not only of a concealed community, but also about their hidden histor

Wavelet Neural Network Methodology for Ground Resistance Forecasting

Motivated by the need of engineers for a flexible and reliable tool for estimating and predicting grounding systems behavior, this study developed a model that accurately describes and forecasts the dynamics of ground resistance variation. It is well-known that grounding systems are a key of high importance for the safe operation of electrical facilities, substations, transmission lines and, generally, electric power systems. Yet, in most cases, during the design stage, electrical engineers and researchers have limited information regarding the terrain’s soil resistivity variation. Moreover, the periodic measurement of ground resistance is hindered very often by the residence and building infrastructure. The model, developed in the present study, consists of a nonlinear, nonparametric Wavelet Neural Network (WNN), trained in field measurements of soil resistivity and rainfall height, observed the past four years. The proposed framework is tested in five different grounding systems with different ground enhancing compounds, so that can be used for the evaluation of the behavior of several ground enhancing compounds, frequently used in grounding practice. The research results indicate that the WNN can constitute an accurate model for ground resistance forecasting and can be a useful tool in the disposal of electrical engineers. Therefore, this paper introduces the wavelet analysis in the field of ground resistance evaluation and endeavors to take advantage of the benefits of computational intelligence