A modification for the calculation of water depth profiles in oil‐treated skin by in vivo confocal Raman microscopy

In this study, an extended calculation method for the determination of the water profiles in oil-treated skin is proposed, which is based on the calculation of the ratio between the Raman band intensities of water (3350-3550 cm−1) and keratin Amide I at 1650 cm−1. The proposed method is compared with the conventional method based on the ratio of the Raman band intensities of water (3350-3550 cm−1) and keratin at 2930 cm−1. The conventional method creates artifacts in the depth profiles of the water concentration in oil-treated skin, showing a lower amount of water in the upper and intermediate layers of the stratum corneum, which is due to the superposition of oil- and keratin-related Raman bands at 2930 cm−1. The proposed extended method shows no artifacts and has the potential to determine the water depth profiles after topical application of formulations on the skin

Feasibility of Unwaxed and Waxed Banana (Musa acuminata x balbisiana) Pseudostem Fibers as Alternative Dental Floss Material

Oral health, waste management, and sustainability are prevalent issues faced by developing countries. Relative to these concerns, there remains a need for oral hygiene essentials that are both effective and environmentally responsible. This study aims to explore the feasibility of banana pseudostem fibers (BPF) as an alternative material for sustainable dental floss in terms of two physical properties, namely, tensile strength and elongation at break. Fibers were mechanically extracted from the outermost sheaths of banana pseudostems to produce two sample groups, unwaxed BPF and waxed BPF, the latter comprising fibers that were coated with a mixture of two parts coconut oil and one part candelilla wax. Both sample groups were tested for tensile strength and elongation at break. According to the mean and SD of both groups and one-way MANOVA, unwaxed BPF had significantly higher tensile strength and elongation at break than waxed BPF, revealing that the wax coating process diminished the physical properties of the BPF due to thermal degradation. Furthermore, the application of the coconut oil-candelilla wax coating was found to have a large effect on tensile strength and a small effect on elongation at break. Results show that there is potential in BPF to be an alternative material for dental floss in relation to the examined properties, although it may not be a substitute for synthetic dental floss material by itself. Modifying the fiber extraction and wax coating processes involved and assessing the chemical properties of the material are also recommended for further research

Benchmarking of cell type deconvolution pipelines for transcriptomics data

Many computational methods have been developed to infer cell type proportions from bulk transcriptomics data. However, an evaluation of the impact of data transformation, pre-processing, marker selection, cell type composition and choice of methodology on the deconvolution results is still lacking. Using five single-cell RNA-sequencing (scRNA-seq) datasets, we generate pseudo-bulk mixtures to evaluate the combined impact of these factors. Both bulk deconvolution methodologies and those that use scRNA-seq data as reference perform best when applied to data in linear scale and the choice of normalization has a dramatic impact on some, but not all methods. Overall, methods that use scRNA-seq data have comparable performance to the best performing bulk methods whereas semi-supervised approaches show higher error values. Moreover, failure to include cell types in the reference that are present in a mixture leads to substantially worse results, regardless of the previous choices. Altogether, we evaluate the combined impact of factors affecting the deconvolution task across different datasets and propose general guidelines to maximize its performance. Inferring cell type proportions from transcriptomics data is affected by data transformation, normalization, choice of method and the markers used. Here, the authors use single-cell RNAseq datasets to evaluate the impact of these factors and propose guidelines to maximise deconvolution performance

Label-free characterization of white blood cells using fluorescence lifetime imaging and flow-cytometry: molecular heterogeneity and erythrophagocytosis [Invited]

Article reporting the results of blood cell characterization using label-free fluorescence imaging techniques and flow-cytometry. Autofluorescence parameters of different cell types – white blood cells, red blood cells, erythrophagocytic cells – are assessed and analyzed in terms of molecular heterogeneity and possibilities of differentiation between different cell types in vitro and in vivo

Methylsulfonylmethane Suppresses Breast Cancer Growth by Down-Regulating STAT3 and STAT5b Pathways

Breast cancer is the most aggressive form of all cancers, with high incidence and mortality rates. The purpose of the present study was to investigate the molecular mechanism by which methylsulfonylmethane (MSM) inhibits breast cancer growth in mice xenografts. MSM is an organic sulfur-containing natural compound without any toxicity. In this study, we demonstrated that MSM substantially decreased the viability of human breast cancer cells in a dose-dependent manner. MSM also suppressed the phosphorylation of STAT3, STAT5b, expression of IGF-1R, HIF-1α, VEGF, BrK, and p-IGF-1R and inhibited triple-negative receptor expression in receptor-positive cell lines. Moreover, MSM decreased the DNA-binding activities of STAT5b and STAT3, to the target gene promoters in MDA-MB 231 or co-transfected COS-7 cells. We confirmed that MSM significantly decreased the relative luciferase activities indicating crosstalk between STAT5b/IGF-1R, STAT5b/HSP90α, and STAT3/VEGF. To confirm these findings in vivo, xenografts were established in Balb/c athymic nude mice with MDA-MB 231 cells and MSM was administered for 30 days. Concurring to our in vitro analysis, these xenografts showed decreased expression of STAT3, STAT5b, IGF-1R and VEGF. Through in vitro and in vivo analysis, we confirmed that MSM can effectively regulate multiple targets including STAT3/VEGF and STAT5b/IGF-1R. These are the major molecules involved in tumor development, progression, and metastasis. Thus, we strongly recommend the use of MSM as a trial drug for treating all types of breast cancers including triple-negative cancers