606 research outputs found
Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in chlamydomonas
Fatty acids are synthesized in the stroma of plant and algal chloroplasts by the fatty acid synthase complex. Newly synthesized fatty acids are then used to generate plastidial lipids that are essential for chloroplast structure and function. Here, we show that inhibition of fatty acid synthesis in the model alga Chlamydomonas reinhardtii activates autophagy, a highly conserved catabolic process by which cells degrade intracellular material under adverse conditions to maintain cell homeostasis. Treatment of Chlamydomonas cells with cerulenin, a specific fatty acid synthase inhibitor, stimulated lipidation of the autophagosome protein ATG8 and enhanced autophagic flux. We found that inhibition of fatty acid synthesis decreased monogalactosyldiacylglycerol abundance, increased lutein content, down-regulated photosynthesis, and increased the production of reactive oxygen species. Electron microscopy revealed a high degree of thylakoid membrane stacking in cerulenin-treated cells. Moreover, global transcriptomic analysis of these cells showed an up-regulation of genes encoding chloroplast proteins involved in protein folding and oxidative stress and the induction of major catabolic processes, including autophagy and proteasome pathways. Thus, our results uncovered a link between lipid metabolism, chloroplast integrity, and autophagy through a mechanism that involves the activation of a chloroplast quality control system.Ministerio de Economía y Competitividad BFU2015-68216-PJunta de Andalucía CVI-7336, BIO2015-74432-JI
Deciphering the function and evolution of the TOR signaling pathway in microalgae
Microalgae constitute a highly diverse group of photosynthetic microorganisms that are widely distributed on Earth. The rich diversity of microalgae arose from endosymbiotic events that took place early in the evolution of eukaryotes and gave rise to multiple lineages including green algae, the ancestors of land plants. In addition to their fundamental role as the primary source of marine and freshwater food chains, microalgae are essential producers of oxygen in the planet and a major biotechnological target for sustainable biofuel production and CO2 mitigation. Microalgae integrate light and nutrient signals to regulate cell growth. Recent studies identified the target of rapamycin (TOR) kinase as central regulator of cell growth and nutrient sensor in microalgae. TOR promotes protein synthesis and regulates processes that are induced under nutrient stress such as autophagy and the accumulation of triacylglycerol and starch. A detailed analysis of representative genomes from the entire microalgal lineage revealed the high conservation of central components of the TOR pathway likely present in the last eukaryotic common ancestor and the loss of specific TOR signaling elements at an early stage in the evolution of microalgae. Here we examine the evolutionary conservation of TOR signaling components in diverse microalgae and discuss recent progress on the study of this signaling pathway in these organisms.Ministerio de Ciencia y Tecnología PGC2018-099048-B-I00, PID2019-110080GB-I0
Incidencia de los dibujos animados de televisión en el aprendizaje sociocognitivo de los niños/as de primaria
We analize the influence of cartoons passed on T.V. in the sociocognitive learning development in Primary Education, as a potencially educational factor.
The phenomenon of cognitive schemas building up, general children¿s development, the social integration process in chilhood at early stages, and TV- learning interaction, are also studied.
Finally, TV and mainly cartoons programmnes are questioned as important learning elements; their possible cognitive and affective effects are also studie
Phosphorus Availability Regulates TORC1 Signaling via LST8 in Chlamydomonas
Target of rapamycin complex 1 (TORC1) is a central regulator of cell growth. It balances anabolic and catabolic processes in response to nutrients, growth factors, and energy availability. Nitrogen- and carbon-containing metabolites have been shown to activate TORC1 in yeast, animals, and plants. Here, we show that phosphorus (P) regulates TORC1 signaling in the model green alga Chlamydomonas (Chlamydomonas reinhardtii) via LST8, a conserved TORC1 subunit that interacts with the kinase domain of TOR. P starvation results in a sharp decrease in LST8 abundance and downregulation of TORC1 activity. A hypomorphic lst8 mutation resulted in decreased LST8 abundance, and it both reduced TORC1 signaling and altered the cellular response to P starvation. Additionally, we found that LST8 levels and TORC1 activity were not properly regulated in a mutant defective in the transcription factor PSR1, which is the major mediator of P deprivation responses in Chlamydomonas. Unlike wild-type cells, the psr1 mutant failed to downregulate LST8 abundance and TORC1 activity when under P limitation. These results identify PSR1 as an upstream regulator of TORC1 and demonstrate that TORC1 is a key component in P signaling in Chlamydomonas.España Ministerio de Economía y Competitividad (grants BFU2015-68216-P and PGC2018-099048- B-100 to J.L.C. and grant BIO2015-74432-JIN to M.E.P.-P.)National Science Foundation (CAREER award MCB-1552522 to L.M.H. and grant MCB-1616820 to J.G.U.)European Commission (grant number 750996
Activation of Autophagy by Metals in Chlamydomonas reinhardtii
Autophagy is an intracellular self-degradation pathway by which eukaryotic cells recycle their own material in response to specific stress conditions. Exposure to high concentrations of metals causes cell damage, although the effect of metal stress on autophagy has not been explored in photosynthetic organisms. In this study, we investigated the effect of metal excess on autophagy in the model unicellular green alga Chlamydomonas reinhardtii. We show in cells treated with nickel an upregulation of ATG8 that is independent of CRR1, a global regulator of copper signaling in Chlamydomonas. A similar effect on ATG8 was observed with copper and cobalt but not with cadmium or mercury ions. Transcriptome sequencing data revealed an increase in the abundance of the protein degradation machinery, including that responsible for autophagy, and a substantial overlap of that increased abundance with the hydrogen peroxide response in cells treated with nickel ions. Thus, our results indicate that metal stress triggers autophagy in Chlamydomonas and suggest that excess nickel may cause oxidative damage, which in turn activates degradative pathways, including autophagy, to clear impaired components and recover cellular homeostasisMinisterio de Economía y Competitividad BFU2012-35913Junta de Andalucía CVI-7336National Institutes of Health GM42143, R24 GM09247
“El gran dia de Girona”, la restauració d’una obra de gran format
La singularitat del procés de conservació-restauració del quadre “El gran dia de Girona”, de Ramon Martí Alsina, rau en l’excepcionalitat de les dimensions de l’obra, però també en què constitueix un referent quant a l’aplicació de criteris molt poc intervencionistes en una pintura d’aquesta envergadura. Aquests han permès preservar les característiques originals del suport malgrat els dos grans estrips que travessaven pràcticament la totalitat de la tela, i respectar l’originalitat de la tècnica pictòrica de l’artista durant un procés tan complex com la neteja de la policromia
J2 effect and the collision restricted three–body problem
The existence of a new class of inclined periodic orbits of the collision restricted three–body problem is shown. The symmetric periodic solutions found are perturbations of elliptic kepler orbits and they exist only for special values of the inclination and are related to the motion of a satellite around an oblate planet
Hip-hop solutions of the 2N-body problem
Hip–Hop solutions of the 2N–body problem with equal masses are shown to exist using an analytic continuation argument. These solutions are close to planar regular 2N–gon relative equilibria with small vertical oscillations. For fixed N, an infinity of these solutions are three–imensional choreographies, with all the in the inertial frame.Peer ReviewedPostprint (author’s final draft
Highly eccentric hip-hop solutions of the 2N-body problem
We show the existence of families of hip-hop solutions in the equal-mass 2N-body
problem which are close to highly eccentric planar elliptic homographic motions of 2N
bodies plus small perpendicular non-harmonic oscillations. By introducing a parameter Є, the homographic motion and the small amplitude oscillations can be uncoupled
into a purely Keplerian homographic motion of fixed period and a vertical oscillation described by a Hill type equation. Small changes in the eccentricity induce large
variations in the period of the perpendicular oscillation and give rise, via a Bolzano argument, to resonant periodic solutions of the uncoupled system in a rotating frame. For small Є ≠ 0, the topological transversality persists and Brouwer's fixed point theorem shows the existence of this kind of solutions in the full system.Postprint (author’s final draft
Proposal of a new indicator to define ductility applied to corroded steel reinforcement on concrete structures = Propuesta de un nuevo indicador para definir la ductilidad aplicada a la corrosión del acero de refuerzo en estructuras de hormigón
The carbonation of concrete or the chlorides ingress in such quantity to reach the level of bars is triggers of reinforcement corrosion. One of the most significant effects of reinforcing steel corrosion on reinforced concrete structures is the decline in the ductility-related properties of the steel. Reinforcement ductility has a decisive effect on the overall ductility of reinforced concrete structures. Different Codes classify the type of steel depending on their ductility defined by the minimum values of several parameters. Using indicators of ductility associating different properties can be advantageous on many occasions. It is considered necessary to define the ductility by means of a single parameter that considers strength values and deformation simultaneously. There are a number of criteria for defining steel ductility by a single parameter. The present experimental study addresses the variation in the ductility of concrete-embedded steel bars when exposed to accelerated corrosion. This paper analyzes the suitability of a new indicator of ductility used in corroded bars
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