11 research outputs found

    Crowdfunding and its use for specific purpose

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    Bakalářská práce na téma "Crowdfunding a jeho využití pro konkrétní záměr" zpracovává návrh crowdfundingové kampaně, zaměřené na spuštění webové stránky, která má za cíl propojit fotbalové kluby, hráče a trenéry na jednom místě. V teoretické části práce je popsán crowdfunding jako pojem, jeho historie a rozdělení. Dále pokračuje popis crowdfundingových platforem a jejich porovnání. V následujících kapitolách jsou detailně popsány fáze crowdfundingové kampaně a její náležitosti. V praktické části je navržena crowdfundingová kampaň dle poznatků z teoretické části a jsou popsány kroky, které by následovaly po ukončení kampaně. V závěru práce je zhodnocen potenciál a omezení navržené crowdfundingové kampaněObhájenoThe bachelor's thesis on "Crowdfunding and its use for a specific project" develops a proposal for a crowdfunding campaign aimed at launching a website that aims to connect football clubs, players and coaches in one place. The theoretical part of the thesis describes crowdfunding as a concept, its history and its classification. It then goes onto crowdfunding platforms description and their comparison. In the following chapters, the phases of a crowdfunding campaign and its essentials are described in detail. In the practical part, a crowdfunding campaign is projected according to the findings of the theoretical part and the steps that would follow after the campaign is completed are described. The thesis concludes by evaluating the potential and limitations of the proposed crowdfunding campaign

    A survey in natural forest ecosystems of Vietnam reveals high diversity of both new and described Phytophthora taxa including P. ramorum

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    In 2016 and 2017, surveys of Phytophthora diversity were performed in 25 natural and semi-natural forest stands and 16 rivers in temperate and subtropical montane and tropical lowland regions of Vietnam. Using baiting assays from soil samples and rivers and direct isolations from naturally fallen leaves, 13 described species, five informally designated taxa and 21 previously unknown taxa of Phytophthora were isolated from 58 of the 91 soil samples (63.7%) taken from the rhizosphere of 52 of the 64 woody plant species sampled (81.3%) in 20 forest stands (83.7%), and from all rivers: P. capensis, P. citricola VII, VIII, IX, X and XI, P. sp. botryosa-like 2, P. sp. meadii-like 1 and 2, P. sp. tropicalis-like 2 and P. sp. multivesiculata-like 1 from Phytophthora major phylogenetic Clade 2; P. castaneae and P. heveae from Clade 5; P. chlamydospora, P. gregata, P. sp. bitahaiensis-like and P. sp. sylvatica-like 1, 2 and 3 from Clade 6; P. cinnamomi (Pc), P. parvispora, P. attenuata, P. sp. attenuata-like 1, 2 and 3 and P. ×heterohybrida from Clade 7; P. drechsleri, P. pseudocryptogea, P. ramorum (Pr) and P. sp. kelmania from Clade 8, P. macrochlamydospora, P. sp. ×insolita-like, P. sp. ×kunnunara-like, P. sp. ×virginiana-like s.l. and three new taxa, P. sp. quininea-like, P. sp. ×Grenada 3-like and P. sp. ×Peru 4-like, from Clade 9; and P. sp. gallica-like 1 and 2 from Clade 10. The A1 and A2 mating types of both Pc and Pr co-occurred. The A2 mating type of Pc was associated with severe dieback of montane forests in northern Vietnam. Most other Phytophthora species, including Pr, were not associated with obvious disease symptoms. It is concluded that (1) Vietnam is within the center of origin of most Phytophthora taxa found including Pc and Pr, and (2) Phytophthora clades 2, 5, 6, 7, 8, 9, and 10 are native to Indochina.info:eu-repo/semantics/publishedVersio

    Population structure of Phytophthora cactorum in Europe

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    The Phytophthora cactorum species complex in Europe is composed of P. cactorum, Phytophthora hedraiandra, and a hybrid species Phytophthora x serendipita. Evolutionary analyses using the amplified fragment length polymorphism (AFLP) method were carried out on 133 isolates from 19 countries. The AFLP data were complemented by sequence analysis of three genes (ITS region of ribosomal RNA gene, phenolic acid decarboxylase Pheca I, and Cytochrome oxidase Cox I), morphometric analysis and cardinal temperature data. The high proportion of clonal genotypes, low gene flow among groups, which was defined by the structure analysis, and low Nei´s gene diversity confirms the homothallic life cycle of the groups. On the other hand, the ITS, Cox I and Pheca I sequence data support occasional hybridization between species. The structure K = 5 grouping revealed two groups of hybrid origin (C2 and F). While the C2 group resembles P. x serendipita, the F group includes Finnish isolates characterized by high oogonial abortion rates and slow growth. The morphological characters routinely used in identification of Phytophthora species are not useful for delimitation of species from the P. cactorum complex. Therefore, we discuss the status of P. hedraiandra as a separate species. The epitypification of P. cactorum is proposed. The reactions of isolates of Phytophthora cactorum, P. nicotianae and P. × pelgrandis to metalaxyl, mancozeb, dimethomorph, streptomycin and chloramphenicol were tested to obtain information about the variability of resistance in these pathogens. Distinct genetic groups showed significant differences in resistance to all tested substances except streptomycin. In response to streptomycin, the growth inhibition rates of distinct groups did not differ significantly. The most remarkable differences were detected in the reactions to chloramphenicol and metalaxyl. Discriminant analysis evaluating the effect of all substances confirmed the differences among the groups, which are in agreement with the differences revealed by earlier DNA analyses

    Mixed-Mating Model of Reproduction Revealed in European Phytophthora cactorum by ddRADseq and Effector Gene Sequence Data

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    A population study of Phytophthora cactorum was performed using ddRADseq sequence variation analysis completed by the analysis of effector genes—RXLR6, RXLR7 and SCR113. The population structure was described by F-statistics, heterozygosity, nucleotide diversity, number of private alleles, number of polymorphic sites, kinship coefficient and structure analysis. The population of P. cactorum in Europe seems to be structured into host-associated groups. The isolates from woody hosts are structured into four groups described previously, while isolates from strawberry form another group. The groups are diverse in effector gene composition and the frequency of outbreeding. When populations from strawberry were analysed, both asexual reproduction and occasional outbreeding confirmed by gene flow among distinct populations were detected. Therefore, distinct P. cactorum populations differ in the level of heterozygosity. The data support the theory of the mixed-mating model for P. cactorum, comprising frequent asexual behaviour and inbreeding alternating with occasional outbreeding. Because P. cactorum is not indigenous to Europe, such variability is probably caused by multiple introductions of different lineages from the area of its original distribution, and the different histories of sexual recombination and host adaptation of particular populations

    Crowdfunding and its use for specific purpose

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    Bakalářská práce na téma "Crowdfunding a jeho využití pro konkrétní záměr" zpracovává návrh crowdfundingové kampaně, zaměřené na spuštění webové stránky, která má za cíl propojit fotbalové kluby, hráče a trenéry na jednom místě. V teoretické části práce je popsán crowdfunding jako pojem, jeho historie a rozdělení. Dále pokračuje popis crowdfundingových platforem a jejich porovnání. V následujících kapitolách jsou detailně popsány fáze crowdfundingové kampaně a její náležitosti. V praktické části je navržena crowdfundingová kampaň dle poznatků z teoretické části a jsou popsány kroky, které by následovaly po ukončení kampaně. V závěru práce je zhodnocen potenciál a omezení navržené crowdfundingové kampaněObhájenoThe bachelor's thesis on "Crowdfunding and its use for a specific project" develops a proposal for a crowdfunding campaign aimed at launching a website that aims to connect football clubs, players and coaches in one place. The theoretical part of the thesis describes crowdfunding as a concept, its history and its classification. It then goes onto crowdfunding platforms description and their comparison. In the following chapters, the phases of a crowdfunding campaign and its essentials are described in detail. In the practical part, a crowdfunding campaign is projected according to the findings of the theoretical part and the steps that would follow after the campaign is completed are described. The thesis concludes by evaluating the potential and limitations of the proposed crowdfunding campaign

    Peronosporales Species Associated with Strawberry Crown Rot in the Czech Republic

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    The symptoms of crown rot on strawberry plants are considered typical for the pathogen Phytophthora cactorum, which causes high losses of this crop. However, an unknown number of related species of pathogens of Peronosporales cause symptoms quite similar to those caused by P. cactorum. To determine their spectrum and importance, strawberry plants were sampled from 41 farms in the Czech Republic. The cultures were isolated from the symptomatic plants using the baiting method, with subsequent cultivation on a semiselective medium. Isolates were identified to the species level using nuclear ribosomal internal transcribed spacer (ITS) barcoding after preliminary morphological determination. In total, 175 isolates of 24 species of Phytophthora, Phytopythium, Pythium, and Globisporangium were detected. The most represented was Phytophthora cactorum, with 113 (65%) isolates, which was recorded in 61% of farms, and the Pythium dissotocum complex with 20 (11%) isolates, which was recorded in 27% of farms. Other species were represented in units of percent. Large differences between farms in the species spectra were ascertained. The differences between species in cardinal growth temperatures and different management of the farms are discussed as a main reason for such a diversification. Regarding the dissimilar sensitivity of various species of Peronosporales against fungicides, the proper determination of the cause of disease is of crucial significance in plant protection

    A Comparison of the Ability of Some Commercially Produced Biological Control Agents to Protect Strawberry Plants against the Plant Pathogen Phytophthora cactorum

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    A comparison of the ability of commercially produced biological control agents—Contans, Gliorex, Hirundo, Polyversum, Prometheus, Clonoplus, Integral Pro and Xilon GR, completed with an isolate of Clonostachys rosea and of Pseudomonas sp.—to protect strawberry plants against Phytophthora cactorum was performed. The experiment was performed on strawberry cultivars Sonata, Karmen, and Wendy—cultivated in a cultivating room and greenhouse. The health of plants was affected negatively by the pathogen in all variants of biological agents used, but differences were seen in the rates of this decrease. The results revealed the ability of some tested agents to improve the growth of plants in the absence of the pathogen; the preparation Polyversum (Pythium oligandrum) was the most beneficial, in both the presence and absence of the pathogen. Contrarily, some agents alone decreased the health of plants; Integral Pro (Bacillus subtillis) and a strain of Pseudomonas sp. caused a deterioration in the health of the plants, even in the absence of a pathogen. The results of our analysis demonstrate the varied usefulness of all agents under unified environmental conditions; their effect seems to be dependent on the conditions and on the combination of the genotypes of all three participants in the interaction: plant–pathogen–antagonist

    Mixed-Mating Model of Reproduction Revealed in European <i>Phytophthora cactorum</i> by ddRADseq and Effector Gene Sequence Data

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    A population study of Phytophthora cactorum was performed using ddRADseq sequence variation analysis completed by the analysis of effector genes—RXLR6, RXLR7 and SCR113. The population structure was described by F-statistics, heterozygosity, nucleotide diversity, number of private alleles, number of polymorphic sites, kinship coefficient and structure analysis. The population of P. cactorum in Europe seems to be structured into host-associated groups. The isolates from woody hosts are structured into four groups described previously, while isolates from strawberry form another group. The groups are diverse in effector gene composition and the frequency of outbreeding. When populations from strawberry were analysed, both asexual reproduction and occasional outbreeding confirmed by gene flow among distinct populations were detected. Therefore, distinct P. cactorum populations differ in the level of heterozygosity. The data support the theory of the mixed-mating model for P. cactorum, comprising frequent asexual behaviour and inbreeding alternating with occasional outbreeding. Because P. cactorum is not indigenous to Europe, such variability is probably caused by multiple introductions of different lineages from the area of its original distribution, and the different histories of sexual recombination and host adaptation of particular populations

    Evaluation of the Ability of Seven Active Ingredients of Fungicides to Suppress <i>Phytophthora cactorum</i> at Diverse Life Stages, and Variability in Resistance Found among Isolates

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    Phytophthora cactorum is considered an important plant pathogen which is causing major damage to strawberry plants worldwide. In the current study, the ability of the active ingredients of seven different fungicides, azoxystrobin, cymoxanil, dimethomorph, fenamidone, fluopicolide, metalaxyl and propamocarb, to suppress the mycelial growth, sporangial formation and zoospore release of P. cactorum isolates, was tested. The variation in resistance against various fungicides was found among the isolates. The active ingredients are also unequally efficient against different life stages of P. cactorum, which is probably associated with their different modes of action. A significant level of resistance was recorded against metalaxyl and dimethomorph; however, these were totally inefficient against the zoospore release, while azoxystrobin did not inhibit mycelial growth. The only fungicide efficient against all three P. cactorum life stages tested was fluopicolide, although the calculated resistance factor gives evidence of the rise of resistance in the majority of isolates even against this fungicide. Significant differences were found between responses to fungicides of isolates from strawberry and from other host species. Based on the Mahalanobis distances calculated in the discriminant analysis comprising all of the assays performed, the similarities among isolates were estimated

    Overview of the COMPASS results

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    COMPASS addressed several physical processes that may explain the behaviour of important phenomena. This paper presents results related to the main fields of COMPASS research obtained in the recent two years, including studies of turbulence, L–H transition, plasma material interaction, runaway electron, and disruption physics: • Tomographic reconstruction of the edge/SOL turbulence observed by a fast visible camera allowed to visualize turbulent structures without perturbing the plasma. • Dependence of the power threshold on the X-point height was studied and related role of radial electric field in the edge/SOL plasma was identified. • The effect of high-field-side error fields on the L–H transition was investigated in order to assess the influence of the central solenoid misalignment and the possibility to compensate these error fields by low-field-side coils. • Results of fast measurements of electron temperature during ELMs show the ELM peak values at the divertor are around 80% of the initial temperature at the pedestal. • Liquid metals were used for the first time as plasma facing material in ELMy H-mode in the tokamak divertor. Good power handling capability was observed for heat fluxes up to 12 MW m−2 and no direct droplet ejection was observed. • Partial detachment regime was achieved by impurity seeding in the divertor. The evolution of the heat flux footprint at the outer target was studied. • Runaway electrons were studied using new unique systems—impact calorimetry, carbon pellet injection technique, wide variety of magnetic perturbations. Radial feedback control was imposed on the beam. • Forces during plasma disruptions were monitored by a number of new diagnostics for vacuum vessel (VV) motion in order to contribute to the scaling laws of sideways disruption forces for ITER. • Current flows towards the divertor tiles, incl. possible short-circuiting through PFCs, were investigated during the VDE experiments. The results support ATEC model and improve understanding of disruption loads
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