Prolactin-RsaI gene polymorphism in East Anatolian Red cattle in Turkey

Prolactin (PRL) plays an important role in regulating mammary gland development, secreting milk, and expressing milk protein genes; making it a potential genetic marker and a candidate gene for production traits in dairy animals. The aim of the study was to determine by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method the gene and genotype frequencies of PRL gene in native East Anatolian Red (EAR) cattle, which are raised as a genetic resource in Turkey. PCR-RFLP analysis involved the use of the RsaI restriction enzyme. Three patterns of fragments were obtained. The AA, AG, and GG genotype frequencies were 0.07, 0.34, and 0.59 in the cattle population, respectively. For Prolactin-RsaI (PRL-RsaI) polymorphism, the population was in Hardy-Weinberg equilibrium. Heterozygosity was found at a medium rate as 0.338 and the calculated FIS value was 0.072.Keywords: genetic resource, mammary gland, prolactin gene, PCR-RFL

Energy absorption in lattice structures in dynamics: Nonlinear FE simulations

An experimental study of the stress–strain behaviour of titanium alloy (Ti6Al4V) lattice structures across a range of loading rates has been reported in a previous paper [1]. The present work develops simple numerical models of re-entrant and diamond lattice structures, for the first time, to accurately reproduce quasi-static and Hopkinson Pressure Bar (HPB) test results presented in the previous paper. Following the development of lattice models using implicit and explicit non-linear finite element (FE) codes, the numerical models are first validated against the experimental results and then utilised to explore further the phenomena associated with impact, the failure modes and strain-rate sensitivity of these materials. We have found that experimental results can be captured with good accuracy by using relatively simple numerical models with beam elements. Numerical HPB simulations demonstrate that intrinsic strain rate dependence of Ti6Al4V is not sufficient to explain the emergent rate dependence of the re-entrant cube samples. There is also evidence that, whilst re-entrant cube specimens made up of multiple layers of unit cells are load rate sensitive, the mechanical properties of individual lattice structure cell layers are relatively insensitive to load rate. These results imply that a rate-independent load-deflection model of the unit cell layers could be used in a simple multi degree of freedom (MDoF) model to represent the impact behaviour of a multi-layer specimen and capture the microscopic rate dependence

Tetrastyryl-BODIPY-Based Dendritic Light Harvester and Estimation of Energy Transfer Efficiency

Cataloged from PDF version of article.Versatile BODIPY dyes can be transformed into bright near-IR-emitting fluorophores by quadruple styryl substitutions. When clickable functionalities on the styryl moieties are inserted, an efficient synthesis of a light harvester is possible. In addition, clear spectral evidence is presented showing that, in dendritic light harvesters, calculations commonly based on quantum yield or emission lifetime changes of the donor are bound to yield large overestimations of energy transfer efficiency

Time domain homogenisation of elastic and viscoelastic metamaterials

The mechanical behaviour of metamaterials typically depends on their microstructural configuration and composition, in addition to their relative density. The design of these materials requires extensive experiments or complex finite element models which tend to be numerically demanding. In order to understand, control and optimise the macroscopic mechanical behaviour, in this paper numerical homogenisation is applied to a simple square unit cell with a single inclusion using a combination of elastic and viscoelastic responses on the micro level. Through a systematic analysis of unit cell behaviour with increasingly complex microstructural configurations, it is shown how certain macroscale constitutive laws can be obtained in a controlled and controllable manner

Probabilistic seismic hazard assessment framework for Uganda: a stochastic event-based modelling approach

Uganda lies between the eastern and western arms of the East African Rift System, the largest seismically active rift above sea level. With increasing population, urbanisation and rapid construction, seismic risk in the country is escalating fast and is compounded by the high vulnerability of the building stock and inadequate disaster prevention and mitigation strategies. Hence, there is an urgent need to assess Uganda’s resilience against seismic risks. This paper presents a Monte-Carlo based probabilistic seismic hazard model for Uganda, as the first step towards the development of a seismic risk and resilience assessment framework for the country. In addition to fault segment data, earthquake catalogues are compiled for the period between 1900 and 2022 to estimate recurrence parameters for source zones in the area of interest. Area source zones incorporating focal mechanisms are used to stochastically model a national hazard framework for Uganda. A logic tree approach is applied to implement four ground motion prediction equations for both stable continental and active shallow crust geologies. Mean hazard curves, uniform hazard spectra, earthquake disaggregation and spectral pseudo-accelerations for major Ugandan cities are derived in addition to hazard maps for the country. The findings are largely consistent with previous regional studies and confirm that western Uganda is exposed to the highest level of seismicity. The model presented herein can be used to kick-start the update and continuous improvement of Uganda Seismic Design Code and the National Policy for Disaster Preparedness and Management

Spatial and temporal averaging in the homogenisation of the elastodynamic response of periodic laminates

This paper suggests a methodology to obtain homogenised material properties from a transient dynamic numerical model. The standard Hill–Mandel Principle, based on spatial averages, is extended with time averaging. Thus, in addition to a sufficiently large Representative Volume Element (RVE) to carry out the averaging in space, a sufficiently large time window is required to carry out the time averaging. The space–time averaging procedure is validated for a periodic laminate bar subjected to a variety of boundary conditions, impedance contrasts and loading conditions. The homogenised results converge to the analytical solutions and confirm that having a higher impedance contrast between laminate components requires not only larger RVE sizes but also longer time averaging windows. The most efficient macroscopic approximation is obtained by a balanced increase in RVE size and time averaging window