Efecto estacional sobre el perfil de ácidos grasos de lípidos totales, fosfolípidos y triglicéridos de Mastacembelus mastacembelus (Lago Atatürk Dam, Turquía)

The present study investigated the lipid content and FA profiles of total lipids, phospholipids (PL) and triacylglycerols (TAG) of Mastacembelus mastacembelus from the Atatürk Dam Lake (Turkey). The results showed that the total lipid content of the dorsal muscle varied seasonally from 0.50% to 3.59%. Wide ranges of saturated fatty acids (SFAs) (36.21 to 50.52%) and polyunsaturated fatty acids (PUFAs) (25.22 to 42.02%) were found in the PL fraction. However, higher monounsaturated fatty acids (MUFAs) (37.63 to 45.07%) and SFA (34.71 to 38.82%) were found in TAG (triacylglycerol). The ratios of ω-3 to ω-6 PUFA ranged from 0.65 to 1.32 and 1.07 to 3.48 in PL (phospholipid) and TAG fraction, respectively. The results also showed that the major components were palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1ω-9), palmitoleic acid (C16:1ω-7), docosahexaenoic acid (DHA, C22:6ω-3), arachidonic acid (AA, C20:4ω-6) and docosapentaenoic acid (C22:5ω-3) in total lipid, C16:0, C18:1ω-9, AA and DHA in PL, C16:0, C16:1ω-7, C18:1ω-9, linoleic acid (LA, C18:2ω-6), and DHA in TAG extracted from the muscle of M. mastacembelus in all seasons.En el presente estudio se investigó el contenido de lípidos y los perfiles de ácidos grasos (FA) de lípidos totales, fosfolípidos (PL) y trigliceridos (TAG) de Mastacembelus mastacembelus del lago Atatürk Dam (Turquía). Los resultados mostraron que el contenido lipídico total del músculo dorsal varió estacionalmente de 0,50% a 3,59%. Se encontraron amplios rangos de ácidos grasos saturados (SFA) (36,21 a 50,52%) y ácidos grasos poliinsaturados (PUFA) (25,22 a 42,02%) en la fracción PL. Sin embargo, se encontraron ácidos grasos monoinsaturados (MUFA) más altos (37,63 a 45,07%) y SFA (34,71 a 38,82%) en TAG (triacilglicerol). Las relaciones de AGPI ω-3 a ω-6 variaron de 0,65 a 1,32 y de 1,07 a 3,48 en PL (fosfolípido) y fracción de TAG, respectivamente. Los resultados también muestran que los principales componentes fueron ácido palmítico (C16:0), esteárico (C18:0), oleico (C18:1 ω-9), palmitoleico (C16:1 ω-7), ácido docosahexaenoico (DHA), C22:6 ω-3), ácido araquidónico (AA, C20: 4 ?-6) y ácido docosapentaenoico (C22: 5 ω-3) en lípidos totales, C16:0, C18:1 ω-9, AA y DHA en PL, C16:0, C16:1 ω-7, C18:1 ω-9, ácido linoleico (LA, C18:2 ω-6) y DHA en el TAG extraído del músculo de M. mastacembelus en todas las estaciones

Preliminary data on COVID-19 in patients with hemoglobinopathies : A multicentre ICET-A study

Objectives: This study aims to investigate, retrospectively, the epidemiological and clinical characteristics, laboratory results, radiologic findings, and outcomes of COVID-19 in patients with transfusion-dependent β thalassemia major (TM), β-thalassemia intermedia (TI) and sickle cell disease (SCD). Design: A total of 17 Centers, from 10 countries, following 9,499 patients with hemoglobinopathies, participated in the survey. Main outcome data: Clinical, laboratory, and radiologic findings and outcomes of patients with COVID-19 were collected from medical records and summarized. Results: A total of 13 patients, 7 with TM, 3 with TI, and 3 with SCD, with confirmed COVID-19, were identified in 6 Centers from different countries. The overall mean age of patients was 33.7±12.3 years (range:13-66); 9/13 (69.2%) patients were females. Six patients had pneumonia, and 4 needed oxygen therapy. Increased C-reactive protein (6/10), high serum lactate dehydrogenase (LDH; 6/10), and erythrocyte sedimentation rate (ESR; 6/10) were the most common laboratory findings. 6/10 patients had an exacerbation of anemia (2 with SCD). In the majority of patients, the course of COVID-19 was moderate (6/10) and severe in 3/10 patients. A 30-year-old female with TM, developed a critical SARS-CoV-2 infection, followed by death in an Intensive Care Unit. In one Center (Oman), the majority of suspected cases were observed in patients with SCD between the age of 21 and 40 years. A rapid clinical improvement of tachypnea/dyspnea and oxygen saturation was observed, after red blood cell exchange transfusion, in a young girl with SCD and worsening of anemia (Hb level from 9.2 g/dl to 6.1g/dl). Conclusions: The data presented in this survey permit an early assessment of the clinical characteristics of COVID 19 in different countries. 70% of symptomatic patients with COVID-19 required hospitalization. The presence of associated co-morbidities can aggravate the severity of COVID- 19, leading to a poorer prognosis irrespective of age

Impaired lung epithelial permeability in hepatitis C virus antibody positive patients detected by 99mTc-DTPA aerosol scintigraphy

Chronic hepatitis C virus (HCV) infection has been recently identified as an aetiological agent in idiopathic pulmonary fibrosis. The present study was designed to determine the pulmonary clearance rate of 99mTc-diethylenetriaminepentaacetic acid (99mTc-DTPA) in asymptomatic HCV antibody positive (HCV Ab+) patients and the role of 99mTc-DTPA aerosol scintigraphy in the early detection of lung involvement. Twenty-six non-smoker HCV Ab+ and HCV-RNA (+) patients (20 female, six male; aged 43±11 years), with no clinical pulmonary symptoms, and normal radiological findings, were studied. Thirty-one healthy non-smoker volunteers (24 female, seven male; aged 40±10 years) were taken as a control group. 99mTc-DTPA aerosol inhalation scintigraphy and pulmonary function tests were performed in all patients and in controls. On the basis of the scintigrams the percentage decline in activity per minute (Kep) was evaluated, which represented an accurate parameter of lung membrane permeability. The mean Kep values of healthy controls (0.78±0.13 for left lung, 0.79±0.14 for right lung) were significantly lower than HCV Ab+ patients (1.10±0.31 for left lung, 1.11±0.34 for right lung, P0.05). We conclude that subclinical alveolitis and/or interstitial lung disease may be present in patients with HCV Ab+, since it is known that an increase in the epithelial permeability of the lung is an early manifestation of interstitial disease. © 2002 Lippincott Williams & Wilkins, Inc

Effects of pesticide use on respiration system of farmers: The case of Middle Anatolia

WOS: 000461384200102

Observational study comparing long-term safety and efficacy of Deferasirox with Desferrioxamine therapy in chelation-naïve children with transfusional iron overload

PubMed ID: 22335829Objectives: An observational study was conducted to explore postmarketing safety and efficacy of Deferasirox (DFX) in comparison with conventional Desferrioxamine (DFO) in chelation-naïve children with transfusional iron overload. Methods: Transfusion-dependent children (aged ?5yr) who had serum ferritin above 1000µg/L and had been prescribed either first-line DFX or DFO for at least 12months to maintain serum ferritin between 500 and 1000µg/L were included. Initial DFX dose was 20mg/kg/d for 7d a week, and DFO dose was 25-35mg/kg/d subcutaneously, given for 5d a week. Dose adjustments were based on serum ferritin changes and safety markers. The primary efficacy endpoint was change in serum ferritin from baseline. The effect of transfusional iron loading rate (ILR) and different doses of chelators on serum ferritin was also assessed. Results: A total of 111 patients were observed for a median of 2.29yr on DFX (n=71) and 2.75yr on DFO (n=40). Absolute change in serum ferritin from baseline to the last available observation was not significant with DFX (91µg/L, P=0.5) but significantly higher with DFO (385µg/L, P<0.005). ILR and DFX doses had a major impact on serum ferritin changes in DFX cohort. The height- and weight-standard deviation scores did not differ significantly in both cohorts during the study. Fluctuations in liver enzymes and non-progressive increase in serum creatinine were the most common adverse events (DFX; 9.8%, 18.0% and DFO; 5.0%, 7.5%, respectively). Conclusion: DFX is well tolerable and at least as effective as DFO to maintain safe serum ferritin levels and normal growth progression in chelation-naïve children. © 2012 John Wiley & Sons A/S

Prevalence of Brucella in Raw Milk: An Example from Turkey

WOS: 000439047000015PubMed ID: 29984724Aim: Brucellosis is a highly contagious zoonotic infection affecting livestock and human beings. This study aimed to investigate the prevalence of Brucella in raw milk collected from a provincial center and central villages in the Central Anatolian region. Materials and Methods: This cross-sectional study was completed between March and September of 2016. The sample size for research was calculated as 263 milk samples with the Epi Info 2000 program. Samples were tested with the milk ring test, Rose Bengal test, and standard Brucella tube agglutination test. Suspicious samples according to these tests were seeded on medium for observation. Results: In this study, 202 cow's milk samples collected from 14 central villages were researched for the presence of Brucella abortus, a Brucella species bacterium. According to the medium seeding results, 35 of 202 raw cow's milk samples ( 17.32%) were identified as suspicious. Conclusion: The research investigated the prevalence of Brucella in milk samples collected from bovine farms used for consumption and production of raw milk products. The most significant infection route in our region is considered to be consumption of milk and milk products such as raw milk and fresh cheese. Especially in rural areas, households consuming their own produced milk are common. In regions with family-style milk and milk product production and consumption, interventional studies with the aim of improving knowledge, attitudes, and behavior related to zoonotic diseases should not be neglected

Y-chromosome polymorphisms in 12 native, Karagül, Karacabey merino breeds from Turkey and Anatolian mouflon (Ovis gmelinii anatolica) [Türkiye’den 12 yerli, karagül, karacabey merinosu ve anadolu yaban koyununda (Ovis gmelinii anatolica) Y-Kromozom polimorfizmleri]

In this study, 182 male animals from 12 native sheep breeds, as well as Karacabey Merino and Karagül breeds of Anatolia, wild sheep Anatolian Mouflon (Ovis gmelinii anatolica) were used as the study material. Based on SRY and SRYM18 regions on the Y-chromosome, haplotypes of the populations were analyzed using DNA sequence analyses. The SRY region, A-oY1 allele was observed in all of the individuals studied. On the other hand, four different alleles corresponding to four Y-chromosome haplotypes were detected at the SRYM18 microsatellite region. Among native Anatolian breeds (n=143), H6 haplotype (80.41%), H4 haplotype (9.09%), H8 haplotype (8.40%) and H12 haplotype (2.1%) were identified. H6 haplotype was observed in all 16 individuals of Ovis gmelinii anatolica. Pairwise FST values based on haplotype frequencies were calculated for domestic sheep, and the highest FST value was observed between Karagül and Kıvırcık along with Karagül and Ovis gmelinii anatolica with pairwise FST value of 0.43202 (P&lt;0.01). Y chromosome polymorphism of sheep from Turkey were examined comparatively with the accumulated data in the literature. Out of seven haplotypes (H4, H5, H6, H7, H8, H12, H19) observed in Europe and Asia, 4 haplotypes (H4, H6, H8 and H12) were observed in Anatolia. H12 was a private haplotype of Sakız, H6 seems to be the predominant haplotype of domestic sheep (79.51%) as well as being the only haplotype observed in Ovis gmelini anatolica. H4 haplotype seemed to be associated with fat tailed sheep migrating to Turkey, entering from south east of Turkey, which may be related with the arrival of nomadic Turks. © 2018, Veteriner Fakultesi Dergisi. All rights reserved.BAP-01-08-2012-014 1176 BAP-01-08-2012-014[1] The present study was supported by both Harran University [project number: 1176] and Middle East Technical University [project number: BAP-01-08-2012-014] -- The present study was supported by both Harran University Scientific Research Committee with the project number 1176 and Middle East Technical University project number BAP-01-08-2012-014. -